Elvin A. Kabat's research while affiliated with Memorial Sloan Kettering Cancer Center and other places

Publications (316)

Article
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Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A(4)(GS I-A(4)), which is cytotoxic to the human colon cancer cell lines, is one of two lectin families derived from its seed extract. It contains only a homo-oligomer of subunit A, and is most specific for GalNAcalpha1-->. In order to elucidate the GS I-A(4)-glycoconjugate interactions in gr...
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The affinity of a lectin from the sponge Geodia cydonium (GCL-I) for multi-antennary Gal beta1-->4GlcNAc and Gal beta1-->3GalNAc ligands was studied by both the biotin/avidin-based microtiter plate lectin binding assay and the inhibition of lectin-glycoform interaction. Among the glycoforms tested for binding, GCL-I reacted strongly with three mult...
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Previous study on the binding properties of a lectin isolated from Codium fragile subspecies tomentosoides (CFT) indicates that this lectin recognizes the GalNAcα1→ sequence at both reducing and nonreducing ends. In this study, the carbohydrate specificity of CFT was further characterized by quantitative precipitin (QPA) and inhibition of lectin-en...
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Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A4(GS I-A4) reacting with the Tn(GalNAc alpha1 --> Ser/Thr) sequence or human blood group Pk active disaccharide (E, Gal alpha1 --> 4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P1 or Tn active glycoproteins were tested...
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Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A4(GS I-A4) reacting with the Tn(GalNAcα1 → Ser/Thr) sequence or human blood group Pk active dissacharide (E, Galα1 → 4Gal, galabiose) was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group P1 or Tn active glycoproteins were tested by QPA, GS IA4...
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The affinity of Bandeiraea (Griffonia) simplicifolia lectin-I isolectin B4 (BSI-B4) for the isomer of human blood group B active disaccharide (B, Gal alpha 1-->3Gal), the Gal alpha 1-->4Gal galabiose ligand, was studied by quantitative precipitin (QPA) and precipitin-inhibition assays. When human blood group B, P1 and H active glycoproteins were te...
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The affinity of Bandeiraea (Griffonia) simplicifolia lectin-I isolectin B-4 (BSI-B-4) for the isomer of human blood group B active disaccharide (B, Galα1→3Gal), the Galα1→4Gal galabiose ligand, was studied by quantitative precipitin(QPA) and precipitin-inhibition assays. When human blood group B, P1 and H active glycoproteins were tested by QPA. BS...
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A human monoclonal antibody (HuA) specific for blood group A substance with two fucose groups was found to be immunochemically almost identical with that of a previously characterized mouse monoclonal anti-A, AC-1001. The VH and VL chain cDNAs of HuA were sequenced and compared with those of AC-1001. The human and mouse antibodies used VH and Vk ge...
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We have been collecting nucleotide and amino acid sequences of proteins of immunological interest, and aligning them in order to understand the structure and function relations of these proteins (1). To aid in organizing and analyzing this collection, a computer program, called SEQHUNT, was written.
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Mouse antidextran monoclonal antibodies showed microheterogeneity which was analyzed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Not only the heavy (H) chains but also the light (L) chains were heterogeneous in terms of isoelectric point (pI). The higher the pI, the more prominent the H chain spots. To demonstrate the cause of...
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We have characterized a human monoclonal IgM kappa, designated IgMDON, from a blood group B individual. IgMDON is specific for alpha-galactosyl residues on blood group B substance; its fine specificity as defined by hemagglutination, quantitative precipitin, and inhibition ELISA assays was for the defucosylated terminal Gal(alpha 1-3)Gal epitope. G...
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Sequences of the third complementarity determining region of antibody heavy chains (CDRH3s) are listed according to their length. Human sequences vary from 2 to 26 amino acids residues, but less extensively in other species. When combined with the other five complementarity determining regions, this enormous length variation of CDRH3, together with...
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The specificity of the anti A+N lectin of Moluccella laevis (MLL) was examined by hemagglutination experiments with enzyme-modified human erythrocytes and by inhibition of hemagglutination. In addition, binding to various glycoproteins and inhibition by different sugars and glycoproteins were examined by enzyme immunoassay with antibodies to the le...
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We have previously noted that a specific amino acid sequence could form the second framework region of human, mouse and rabbit immunoglobulin light chains, suggesting that this sequence has been preserved for 80 million years. Through divergent evolution, each species has acquired a different set of framework region sequences; however, these sets s...
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T-cell receptor (Tcr) alpha chains are classified into four subgroups (I, II, III, and miscellaneous) based on the amino acid residues at positions 61 and 62. Subgroup I has Gly Phe at these positions, subgroup II has Arg Phe, subgroup III has Arg Leu, and subgroup miscellaneous has several other combinations. Variability plots for subgroups I, II,...
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We have characterized a monoclonal isogeneic antiidiotype, IdB5.7, from a BALB/c mouse immunized with the anti-alpha(1----6)dextran C57BL/6 45.21.1. It defined a hapten-inhibitable idiotope expressed on four of the 2 myeloma and 37 hybridoma anti-alpha(1----6)dextrans tested. Sequence comparison of Id+ and Id- anti-alpha(1----6)dextrans suggested t...
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Publisher Summary This chapter describes the modeling of the antigen-binding sites of antibodies. The chapter reviews the various modeling procedures that have been applied to antibodies, evaluates the success of these procedures in predicting combining site structures, and discusses potential improvements and problems. The specificity of antibody–...
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A cDNA clone that encodes the heavy chain variable region (VH) of an IgM M-protein with anti-myelin-associated glycoprotein (MAG) activity secreted by chronic lymphocytic leukemia cells (B-C11) from a patient with peripheral neuropathy was cloned and sequenced. The JH region was identical to the germline JH4 sequence except for deletion of a thymid...
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Two monoclonal IgG3 syngeneic anti-idiotypes are described which form soluble and insoluble complexes with anti-alpha(1----6)dextran hybridoma and myeloma proteins. Specific precipitation was seen when purified anti-alpha(1----6)dextrans were added to ascitic fluid containing IgG3 kappa anti-idiotype. Analysis of the supernatants of the idiotype-an...
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Bacteria were engineered for the expression of mouse immunoglobulin light chain variable region (VL) and heavy chain variable region (VH) fusion proteins. cDNAs encoding the VL and VH of anti-alpha(1----6)dextran hybridoma protein 19.22.1 were inserted into the pATH 10 prokaryotic expression vector downstream of trp operon sequences. V domains join...
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The carbohydrate binding specificity of the basic lectin from winged bean (Psophocarpus tetragonolobus) was investigated by quantitative precipitin analysis using blood group A, B, H, Le and I substances and by precipitation inhibition with various mono- and oligosaccharides. The lectin precipitated best with A1 substances and moderately with B and...
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We have observed that antidextran hybridomas with potential N-linked glycosylation sites in VH have higher affinity for polymeric dextran and for isomaltoheptaose than those lacking potential glycosylation sites. In these studies we have used gene transfection and expression techniques to verify that the carbohydrate addition sites in VH were used....
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The combining site of Ricinus communis agglutinin (RCA1) was studied by quantitative precipitin and precipitin inhibition assays. Of 31 complex carbohydrates tested, all except active and inactive antifreeze glycoproteins, Streptococcus group C polysaccharide, and native rat salivary glycoprotein, reacted strongly, and 22 completely precipitated th...
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The specificities of polyclonal and monoclonal anti-idiotypes to three anti-alpha(1----6)dextrans-10.16.1, QUPC52, and W3129--were examined by competition ELISA. A major idiotype was defined by two polyclonal and two monoclonal anti-idiotypes to 10.16.1, and a polyclonal anti-idiotype to QUPC52. Another monoclonal anti-idiotype to 10.16.1 defines a...
Article
Knowledge of monoclonal antibody combining sites and of their specificities and three dimensional structures has been obtained using a variety of materials and sources: These include (1) collections of monoclonal immunoglobulins of various classes from patients with multiple myeloma and Waldenstrom macroglobulinemia (Eisen et al., 1967; Seligmann a...
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Six hybridomas, five from C58/J and one from C57BL/10 nu/nu, immunized with stearyl-isomaltotetraose (S-IM4) were established. One produced IgG3, one IgM and four IgA. The specificities and sizes of the antibody combining sites were determined by quantitative precipitin and ELISA quantitative inhibition assays. All cross-react with alpha(1----6)dex...
Chapter
Despite the enormous progress of molecular biology in bringing understanding of the generation of antibody complementarity and diversity to the gene level, we do not yet have a comprehensive picture of which individual amino acid side chains are functionally important, e.g., involved in determining antibody complementarity either by contacting the...
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The combining site of the lima bean (Phaseolus lunatus) lectin (LBL) was studied by quantitative precipitin and precipitin-inhibition assays. The lectin precipitated best with hog gastric mucosa and human ovarian cyst blood group A1 substances and moderately with A2 substances. B substances precipitated very poorly and H, Lea, Leb, and precursor I...
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A dot assay on nitrocellulose was developed for screening hybridoma fusions for the presence of anti-idiotopes. The technique involves spotting of hybridoma supernatants onto nitrocellulose filters, and detection of anti-idiotope with an alkaline phosphatase-coupled idiotype.
Article
Human blood-group A active glycoproteins from ovarian-cyst fluid were subjected to Smith degradation and subsequent beta-elimination. The resulting oligosaccharide-alditols represent the core and backbone domains of the O-linked carbohydrate chains. Nine of these, ranging in size from disaccharides to hexasaccharides, were investigated by 1H-NMR sp...
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The specificities of the combining sites of 19 mouse monoclonal antibodies to dextran B1355S have been characterized immunochemically by quantitative precipitin and precipitin inhibition assays; association constants for B1355S were determined by affinity gel electrophoresis. Cross-reactive and individual idiotypes related to the BALB/c B1355S-bind...
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Examination of the gel electrophoresis patterns of 14C-biosynthetically labeled immunoglobulin from C57BL/6 × BALB/c IgA hybridomas reveals that each of the monoclonal cell populations produces two different forms of IgA: molecules with heavy chains (H) and light chains (L) joined by disulfide bonds, as well as molecules with H and L being noncoval...
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Twelve C57BL/6J hybridoma clones, 9, 2 and 1 from mice immunized with stearyl-isomaltotetraose, stearyl-isomaltopentaose and stearyl-isomaltohexaose respectively were characterized. Seven produced IgA and 5 IgM. The specificities and sizes of their combining sites were determined by quantitative precipitin and precipitin inhibition assays. All 12 h...
Chapter
Editorial note (G.M.W.C.). — During the 9th International Subcellular Methodology Forum, Dr. Kabat, as well as delivering the keynote address, celebrated his 70th birthday. Forum participants were privileged to listen to a retrospective look at the way in which our concepts of antibody combining site structure have developed over the past 50 years,...
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Evidence is provided that the trisaccharide beta DGal(1----4)beta DGlcNAc(1----6)beta DGal is bound by the monoclonal anti-I Ma antibody beginning with a basically nonpolar cleft at the surface of the protein which comes into contact with a weakly polar region of the trisaccharide that extends from the C-5 methylene group of the reducing unit about...
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We have studied the specificities of two human monoclonal, IgM containing sera, s/IgMMAC and s/IgMFIS, from patients with polyneuropathy. s/IgMMAC precipitates only with chondroitin sulfate C and not with A and B whereas s/IgMFIS is precipitated by chondroitins A, B (dermatan sulfate), and C. Inhibition assays using 2-acetamido-2-deoxy-3-O-(4-deoxy...
Chapter
Idiotypic determinants, idiotopes, are best defined as antigenic determinants located on the Fv fragment of an antibody molecule. Since the demonstration that myeloma antibodies from different individuals showed characteristic distinct antigenic specificities(1,2) and the subsequent findings that such antigenic determinants were present on induced...
Chapter
A major problem of the present decade is the elucidation of the structural basis of antibody complementarity, or, in other words, what do antibody combining sites of a given specificity look like and how do the various amino acid side chains make for different kinds of combining sites (1–3). Intimately related to this is the question of how the cap...
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The tetrasaccharide-alditol, RL 0.8, from one stage of Smith degradation of Klebsiella K33, was subjected to Smith degradation to yield a disaccharide-alditol. The purified disaccharide-alditol was characterized by sugar analysis, methylation analysis, and mass spectrometry. The following structure was found. (formula see text)
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The specificity of the Griffonia simplicifolia IV (GS-IV) lectin was studied by quantitative precipitin and quantitative precipitin inhibition assays. The lectin precipitated most strongly with a human H,Leb blood group substance and reacted strongly with an Lea and an A2 blood group substance with Leb activity. Because of the heterogeneity of the...
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The capsular polysaccharide of Klebsiella K33 was subjected to Smith degradation, and an oligosaccharide-alditol, RL 0.8, was isolated. The purified oligosaccharide-alditol was characterized by sugar and methylation analyses, and by mass spectrometry as the permethylated derivative, and found to have the following structure.
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The lectin of Dolichos biflorus, a hemagglutinin previously considered to be blood group A specific, is now found to react much more strongly with the terminal disaccharide unit [alpha DGalNAc(1 leads to 3) beta DGalNAc] of the Forssman antigenic determinant. In contrast, the relative reactions of the lectins of Helix pomatia (which also agglutinat...
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The combining site of the Erythrina cristagalli lectin was studied by quantitative precipitin and precipitin inhibition assays. The lectin precipitated best with two fractions of a precursor human ovarian cyst blood group substance with I and i activities. A1, A2, B, H, Lea, and Leb blood group substances precipitated poorly to moderately and subst...
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Article
Carbohydrate structures in the interior of a blood group A active substance (MSS) were exposed by one and by two Smith degradations. Reactivities of the original glycoprotein and its Smith degraded products with 13 different lectins and with anti-I Ma were studied by quantitative precipitin assay. MSS and its first Smith degraded product completely...
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Precipitin reactions of two human monoclonal macroglobulins specific for Klebsiella capsular (K) polysaccharides containing 3,4-pyruvylated d-galactose (K30) and 4,6-pyruvylated d-galactose (K21) have been studied over a pH range of 4.0–7.0. One macroglobulin, IgMWEA, that does not precipitate at pH 7.0 with Klebsiella K21 polysaccharide containing...
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Binding constants of monomers of seven BALB/c IgM, four BALB/c IgA, and one C57BL/6 IgA anti-alpha (1 leads to 6) dextran hybridoma antibodies with dextran B512 and with isomaltoheptaose were determined by affinity electrophoresis. Bindings constants to dextran range from 1.52 X 10(5) to 4.43 X 10(5) ml/g for the five IgA monomers and from 1.70 X 1...
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The combining sites of seven BALB/c IgM, four BALB/c IgA and one C57BL/6 IgA hybridoma antibodies specific for alpha (1 leads to 6) linked dextran were probed by precipitin and precipitin inhibition assays. The 12 antibodies are able to bind to linear determinants in the interior of the dextran molecule; some have sites complementary to six alpha (...
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The four snail galactans studied are polysaccharides of high molecular weight that are composed entirely of d- and l-galactosyl residues. AaG and CnG, which had not previously been studied, are highly branched galactans composed mainly of (1→3)- and (1→6)-linked galactosyl residues, as shown by the results of periodate oxidation and permethylation...
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The combining site of purified Vicia villosa lectin was studied by quantitative precipitin and precipitin inhibition assays. The lectin, which specifically hemagglutinated blood group A erythrocytes nevertheless precipitated to different extents with blood group A1, A2, H, B and precursor I substances from saliva and ovarian cysts, and with differe...
Article
An antiserum to stearyl-IM5 liposomes, R-856, with a high proportion of IgM and IgG antibodies was separated into IgM and IgG fractions by column chromatography. The IgM and IgG antibody binding sites were studied by quantitative precipitin, precipitin inhibition and competitive binding assays. Both were similar to unfractionated antibodies with si...
Article
The specificities and the sizes and shapes of the antibody combining sites of the 15 antisera raised against various stearyl-isomaltosyl oligosaccharides were studied by quantitative precipitin and precipitin inhibition. The antibodies precipitated well with dextrans B512 and B1424 but less well with B1299S and B1355S. Only 3 of the 15 antisera rea...
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Further studies of the inhibition of the monoclonal anti-I Ma (group 1) antibody, in quantitative precipitin assays, by synthetic oligosaccharides derived from N-acetyllactosamine (LacNAc) are reported. The results confirm that the antibody binds about the βLacNAc-OCH2CHO-portions of such structures as βLacNAc(1→6)βdGal or βLacNAc(1→6)αdGalNAc. The...
Article
Twelve mouse hybridomas secreting antibodies to dextran B512, identified by replica immunoadsorption screening of 100,000 immobilized hybridoma clones, were obtained. Among 11 hybridomas of BALB/c origin seven produce IgM and four produce IgA. One hybridoma of C57BL/6 origin synthesizes IgA. A κ light chain is synthesized by each of the 12 hybridom...
Article
Maclura pomifera lectin was purified from crude extracts of seeds by adsorption onto soluble polyleucyl hog A + H blood group substance and elution with either melibiose or N-actyl-d-galactosamine. The purified lectin formed a single band in immunodiffusion and immunoelectrophoresis against rabbit antiserum to the crude extract. It migrated as a br...
Article
Sophora japonica lectin agglutinates human B erythrocytes strongly and A1 erythrocytes weakly. Bivalent metal ions such as Ca2+, Mn2+, or Mg2+ were shown to be essential for hemagglutinating and precipitating activities. At optimal concentrations of bivalent metal ions, hemagglutinating activity was highest between pH 8.5 and 9.0 and decreased shar...
Chapter
This chapter discusses the hypothesis that the nucleotides coding for the FR segments are minigenes, a minigene being defined as a gene coding for a segment of a polypeptide chain, and that complete V regions are assembled somatically by the assortment of FR and CDR minigenes. The studies on mouse λ clones confirmed the hypothesis of the somatic as...
Article
The association constants for the interaction of BALB/c (UPC 10, Y5476, W3082, and UPC 61) and NZB (PC 3660) myeloma anti-D-fructans in pure form, or in ascitic fluids, with high-molecular-weight levans (Ka) and with such low-molecular-weight compounds as rye-grass levan, inulin, sucrose, and D-fructo-oligosaccharides (Kia) were determined by affin...
Article
Nylon fibers coated with various lectins were used for the specific selection from mixed populations of erythrocytes or tissue culture cells with lectin receptors. Binding of human group O red blood cells to fibers treated with Ulex europaeus lectin I (H-specific) or of human group A red cells to fibers treated with Helix pomatia lectin (A-specific...
Article
Guidelines for submitting commentsPolicy: Comments that contribute to the discussion of the article will be posted within approximately three business days. We do not accept anonymous comments. Please include your email address; the address will not be displayed in the posted comment. Cell Press Editors will screen the comments to ensure that they...
Article
The specificity of Wistaria floribunda hemagglutinin, purified by adsorption on insoluble polyleucyl hog gastric mucin blood group A + H (PL-hog A + H) substance, elution with lactose, and gel filtration on Sephadex G-200, was studied immunochemically by quantitative precipitin and precipitin inhibition assays. The purified hemagglutinin, with a mo...
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This chapter discusses the basic concepts related to antigen–antibody reactions. An antigen is any substance that, when introduced parenterally into an animal, will induce the formation of antibodies. The antibody formed is generally found in serum or other biological fluids and should react with the antigen used to induce its formation. The term i...
Article
The specificity of two isolectins, A4 and B4, of Bandeiraea simplicifolia lectin I (BS-I) was studied by quantitative precipitin, precipitin inhibition, as well as by competitive binding assays using various blood group substances and tritium-labeled human B substance. A4 precipitated well with A1, A2, B, and precursor substances, with A2 precipita...
Article
The secondary structure of the lac repressor protein proposed by Chou et al. has been modified to include the recent revisions in sequence. In addition to the Chou and Fasman method, five other methods were used; they include those of (1) Lim, (2) Ptitsyn and Finkelstein, (3) Burgess et al., (4) Bunting et al., and (5) Wu and Kabat. Any two individ...
Article
A blood-group-I determinant reacting specifically with anti-I Ma serum (group 1) has been produced biosynthetically by the action of a beta-galactosyl transferase isolated from human milk on a precursor glycoprotein produced by formic acid hydrolysis and beta-D-galactosidase action on blood-group H substance prepared from hog gastric mucosa.
Article
The lectin II from Ulex europaeus seeds was purified by adsorption on insoluble polyleucyl hog A + H blood group substance and elution with 35% ethylene glycol, and by chromatography on ϵ-aminocaproyl-fucosyl-amine-agarose. In immunodiffusion against rabbit antiserum to the crude extract, the isolated lectin formed one line which fused with one of...
Article
Immunochemical and chemical studies were used to monitor and evaluate the structural changes produced by an enzyme from Turbo cornutus in periodate-oxidized and Smith-degraded, human blood-group substances from ovarian cysts. After the first step of periodate oxidation and Smith degradation, two blood-group substances, JS (HLeb and N-1 (Lea), were...
Article
The specificity of Bandeiraea simplicifolia lectin I (BS I) has been studied by competitive-binding assays (CBA) using tritium-labeled human B and hog A substances. Blood-group B substances isolated from horse gastric mucosae and from human ovarian-cyst fluids were much better inhibitors of binding of tritiated blood-group B substance to insoluble...
Article
Two dextran-specific (PC 3858 and PC 3936) and one levan-specific (PC 3660) NZB myeloma proteins were studied by quantitative precipitin and precipitin-inhibition assays. Both myeloma antidextrans were alphaD-(1 leads to 6) specific and precipitated strongly with a synthetic, linear dextran, molecular weight 35,500, and with other dextrans. The two...
Article
When washed cells from human semen samples were plated out, epithelial cultures were obtained. The human ejaculates used as starting material contained, in addition to spermatazoa, 10(3) to 10(7) cells of other types, including granulocytes, macrophages, lymphocytes, spermatocytes and epithelial cells. Although no fractionation of cell types was at...
Article
Sequences of each of the four framework segments FR1, FR2, FR3, and FR4 of the variable regions (V-regions) of light and heavy chains of immunoglobulins were grouped into sets with identical sequences. Sets contained from 1 to 18 members. When each V-region was traced from one FR to the next, it was seen that members of the same set in FR1 could be...
Article
The extraordinarily large number of immunoglobulins renders them an intriguing class of molecules for attempts to predict their conformations. The predictive method applied, using a 20 × 20 table of the observed effects of nearest-neighboring amino acids on the conformation (Φ,Ψ angles) of the middle residue in known proteins, indicates positions o...
Article
The specificity of purified Ulex lectin I has been studied by quantitative precipitin, quantitative precipitin inhibition, and competitive binding assays using tritium-labeled hog mucin H substance. The lectin is precipitated by human and hog H substances, by human A2 substances, by a cow substance with A activity, and by B substances of human and...
Article
The most important problem in structural immunology and immunochemistry is an understanding of antibody complementarity in terms of three-dimensional structure as this should provide new insights leading to the genetic basis for the generation of diversity and will open new perspectives for research in cellular immunology. Antibody complementarity...
Article
A comparison is made of the specific combining sites of a number of lectins and of antibodies with emphasis on those reacting with blood group A, B, and H determinants. The ranges of site sizes and specificities of both groups are similar both from immunochemical studies and from the limited x-ray diffraction data available.
Article
The lectin from Euonymus europeus seeds was purified by adsorption onto insoluble polyleucyl hog A + H blood group substance and subsequent elution with lactose. The isolated lectin formed three lines in immunoelectrophoresis against rabbit antisera to the crude seed extract and showed three components on electrophoresis in acrylamide gel at pH 9.4...
Article
The lectins from the sponge Aaptos papillata were isolated by affinity chromatography using polyleucyl blood group A + H substances from hog stomach linings as an absorbent and eluting with 3 M MgCl2. Further separation on diethylaminoethylcellulose and preparative disc electrophoresis on polyacrylamide gave the three fractions, Aaptos lectins I, I...
Article
The specificity of purified, peanut agglutinin has been studied immunochemically by quantitative precipitin and inhibition assays. The lectin showed substantial differences in precipitating with blood-group substances of the same specificity. Of the B substances tested, horse 4 25% completely precipitated the lectin, Beach phenol insoluble failed t...
Article
Purification of the oligosaccharides obtained by degradation with alkaline borohydride of two fractions of blood group substance from ovarian cyst fluid differing in B, I, and i activities and reactivity toward concanavalin A, was achieved by Bio-Gel P-2, charcoal-Celite, paper and high pressure liquid chromatography. Characterization of twelve oli...
Article
The hemagglutinins from the sponge Axinella polypoides were isolated by affinity chromatography using Sepharose 4B as an absorbent and eluting with DGal. Further separation on DEAE-cellulose and preparative disc electrophoresis on polyacrylamide and agarose gave three fractions. The physicochemical properties and binding specificities of the two ma...
Article
Blood group substance was isolated from ovarian cyst fluid of a Nigerian, Tij, by digestion with pepsin, precipitation by ethanol, solution in 90% phenol, and fractional precipitation with ethanol from phenol. There was a progressive decrease in fucose and galactose and in B activity and an increase in I activity of group 3 (anti-I Step) and i acti...
Article
Oligosaccharides from base-borohydride-treated B-active and non-B-active glycoproteins of horse stomach mucosae were purified chromatographically on Bio-Gel P-2, charcoal-Celite, paper and high pressure liquid chromatography. From colorimetric and gas-liquid Chromatographic analyses, methylation, quantitative periodate oxidation and Smith degradati...