Elizabeth L. Bell's research while affiliated with Manchester Institute of Studies and other places

Publications (4)

Preprint
The recent discovery of a hydrolytic enzyme, Is PETase, that can deconstruct poly(ethylene) terephthalate (PET), has sparked great interest in biocatalytic approaches to recycle plastics. Realisation of commercial utility will require the development of robust engineered enzymes that meet the demands of industrial processes. Although rationally eng...
Preprint
Full-text available
The recent discovery of a hydrolytic enzyme, IsPETase, that can deconstruct poly(ethylene) terephthalate (PET), has sparked great interest in biocatalytic approaches to recycle plastics. Realisation of commercial utility will require the development of robust engineered enzymes that meet the demands of industrial processes. Although rationally engi...
Article
Biocatalysis has become an important aspect of modern organic synthesis, both in academia and across the chemical and pharmaceutical industries. Its success has been largely due to a rapid expansion of the range of chemical reactions accessible, made possible by advanced tools for enzyme discovery coupled with high-throughput laboratory evolution t...
Article
Full-text available
Directed evolution requires the creation of genetic diversity and subsequent screening or selection for improved variants. For DNA mutagenesis, conventional site-directed methods implicitly utilise the Boolean AND operator (creating all mutations simultaneously), producing a combinatorial explosion in the number of genetic variants as the number of...

Citations

... This directed evolution procedure afforded a HotPETase variant with 21 mutations compared to wild-type IsPETase and a T m of 82.5°C. 89 Therefore, the thermostability of IsPETase and other homologous enzymes depends on the interplay of many effects, necessitating further comprehensive research. ...
... This can be accomplished through a combination of enzyme discovery and protein engineering. In fact, future developments in protein engineering are likely to have the most profound impact on industrial biocatalysis (Bell et al., 2021). ...
... In an attempt to modulate the protein's affinity, it was decided that a smaller library with mutations in specific residues would be most prudent. One method to create a smaller library that still screens a wide array of potentially beneficial mutants is to use a Boolean OR-type library 31 . ...