Elisabeth Bock's research while affiliated with Medical University of Graz and other places

Publications (20)

Article
The cover image is based on the Research Article The complex synaptic pathways onto a looming-detector neuron revealed using serial block-face scanning electron microscopy by Stefan Wernitznig et al., https://doi.org/10.1002/cne.25227.
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The ability of locusts to detect looming stimuli and avoid collisions or predators depends on a neuronal circuit in the locust's optic lobe. Although comprehensively studied for over three decades there are still major questions about the computational steps of this circuit. We used 4th instar larvae of Locusta migratoria to describe the connection...
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Whilst the significance of substrate topography as a regulator of cell function is well established, a systematic analysis of the principles underlying this is still unavailable. Here we evaluate the hypothesis that surface energy plays a decisive role in substrate-mediated modulation of cell phenotype by evaluation of cell behaviour on synthetic m...
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Investigations of the ultrastructural features of neurons and their synapses are only possible with electron microscopy. Especially for comparative studies of the changes in densities and distributions of such features, an unbiased sampling protocol is vital for reliable results. Here, we present a workflow for the image acquisition of brain sample...
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Histological processing of thermosensitive electrospun poly(ε-caprolactone)/poly(l-lactide) (PCL/PLA) scaffolds fails, as poly(ε-caprolactone) (PCL) is characterized by its low-melting temperature (Tm = 60 °C). Here, we present an optimized low-temperature preparation method for the histological processing of un-/cellularized thermosensitive PCL/PL...
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Environmental enrichment (EE) refers to the provision of a complex and stimulating housing condition which improves well-being, behaviour and brain function of laboratory animals. The mechanisms behind these beneficial effects of EE are only partially understood. In the current report, we describe a link between EE and neuropeptide Y (NPY), based o...
Article
The small size of some insects, and the crystalline regularity of their eyes have made them ideal for large-scale reconstructions of visual circuits. In phylogenetically recent muscomorph flies, like Drosophila, precisely coordinated output to different motion-processing pathways is delivered by photoreceptors (R cells), targeting four different po...
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Background: The numerical density of synapses and their ultrastructural features are best assessed with electron microscopy. Counting is done within counting frames placed on a pair of sections (disector technique). But this requires that the thin sections are taken from comparable brain regions and the disectors are placed in a uniform random fas...
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Morphology and behaviour of third instar larvae of the Holomediterranean antlion species Myrmecaelurus trigrammus (Pallas) are described. Larvae are facultative pit-builders, they either ambush their prey at the surface, or dig pitfall traps that prey fall in to. Dark brown spots on dorsal and ventral sides of the head and on dorsal side of the tho...
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Transient receptor potential ankyrin 1 (TRPA1) channels are expressed by primary afferent neurones and activated by irritant chemicals including allyl isothiocyanate (AITC). Here we investigated whether intracolonic AITC causes afferent input to the spinal cord and whether this response is modified by mild colitis, morphine or a TRPA1 channel block...
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The Tyrolean iceman, a 5300-year-old mummy, presents tattoos on different parts of his body. Skin samples of several line tattoos and a cross tattoo were investigated by optical microscopy and various electron microscopy techniques (TEM, EFETEM, EELS, EDXS, electron diffraction). The epidermis of the investigated skin areas was mostly lacking. The...
Article
There is a gender-related comorbidity of pain-related and inflammatory bowel diseases with psychiatric diseases. Since the impact of experimental gastrointestinal inflammation on the emotional-affective behavior is little known, we examined whether experimental gastritis modifies anxiety, stress coping and circulating corticosterone in male and fem...
Article
Acid challenge of the gastric mucosa is signaled to the brainstem. This study examined whether mild gastritis due to dextrane sulfate sodium (DSS) or iodoacetamide (IAA) enhances gastric acid-evoked input to the brainstem and whether this effect is related to gastric myeloperoxidase activity, gastric histology, gastric volume retention or cyclooxyg...
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Vagal afferents play a role in gut-brain signaling of physiological and pathological stimuli. Here, we investigated how backdiffusion of luminal HCl or NH(4)OH and pentagastrin-stimulated acid secretion interact in the communication between rat stomach and brain stem. Rats were pretreated intraperitoneally with vehicle or appropriate doses of cimet...

Citations

... The feedback mechanism has been proven to effectively improve model performance for many computer vision tasks, such as visual masking [8], small object detection [9], figure-ground segregation [10], contour integration [11], object recognition [12]. It has been found that feedback loops may exist in the locust vision systems [13,14]. However, the corresponding feedback neural computation has never been investigated. ...
... This decrease was mainly related to a reduction of the dispersive component of the surface energy, since the polar component was not significantly changed. The similar data on reducing surface energy for micropatterned polymer materials were also observed by [42]. FL immobilization on the patterned PDMS substrates resulted in the slight increase in total surface energy, but its polar component remained in the range of 0.1-3.7 mJ/m 2 ( Table 2). ...
... An unbiased sampling protocol described earlier (JoVE Video Dataset; Wernitznig et al. 2019) automatically selects 60 random locations on each sample determined using a random number generator. The elemental maps were accompanied by overview micrographs taken from the same locations using the bright field mode at 3500× magnification. ...
... Radial and circumferential sections stained with hematoxylin and eosin stain (H&E) showed that the PCL tissue-engineered constructs had an evident trilayer structure, with distinct regions of aligned and randomly oriented cells and collagen fibrils (figures 8(a) and (b)). Due to the temperature sensitivity of PCL polymer, the sample embedding (in paraffin) process partially deformed the PCL substrate in the PCL tissue constructs [52]. Similarly, H&E stained PLLA tissue-engineered constructs showed trilayer, oriented structure, with distinct regions of aligned and randomly oriented cells and collagen fibrils (figures 8(c) and (d)). ...
... Endocrine and other signaling molecules likely play a role. Studies of individual differences of stress-coping indicate that region-specific glucocorticoid and neuropeptide expression influence active versus passive strategies 47,48 . ...
... Conventional transmission electron microscopy allows the counting of profiles of synapses on the sections, and this was recently used to show that potassium 2-(l-hydroxypentyl)-benzoate has a protective effect on synapse numbers in APP/PS1 mice [48]. Moreover, using a dissector as a counting frame on pairs of sections [49] allows unbiased quantification of the numbers of structures in a certain volume without the need for 3D reconstructions (reviewed in [50]), as has been performed for synapse numbers (e.g., [51][52][53][54][55][56][57]), or even immunolabelled cell types [58] within the period covered by this review. ...
... Regarding invertebrates, there has also been significant progress on crabs (Carbone, Yabo and Oliva, 2018), insects including various dipterans (blowfly (Nordström, 2012), hoverfly Nordström, Barnett and O'Carroll, 2006), dragonfly (Dunbier, Wiederman, Shoemaker and O'Carroll, 2012), fruit fly Drosophila (Joesch, Schnell, Raghu, Reiff and Borst, 2010;Strother, Wu, Wong, Nern, Rogers, Le, Rubin and Reiser, 2017;Arenz, Drews, Richter, Ammer and Borst, 2017;Fisher, Leong, Sporar, Ketkar, Gohl, Clandinin and Silies, 2015;Drews, Leonhardt, Pirogova, Richter, Schuetzenberger, Braun, Serbe and Borst, 2020;Borst, 2018), locusts (Wernitznig, Rind, Polt, Zankel, Pritz, Kolb, Bock and Leitinger, 2015;Simmons, Sztarker and Rind, 2013;Peron, Jones and Gabbiani, 2009;Jones and Gabbiani, 2010)). Here the progress on ON/OFF channels in motion perception will be highlighted upon fruit fly Drosophila and locust as prevailing system paradigms. ...
... The study was carried out with third instar larvae. The same protocol of Devetak et al. (2010aDevetak et al. ( , 2010bDevetak et al. ( , 2013 was implemented. All specimens were fixed in glutaraldehyde and paraformaldehyde in PBS and washed in a buffer of 1% osmium in water. ...
... These aesthetical and medical applications require different types of inks and colors. This is why tattoos are currently not limited to black ink (carbon black), and the use of different inks, even though it has increased recently, go back a long way (9). Thus, tattoos are generally based on a wide range of colored substances in suspension and these mixtures of substances have evolved over time to improve the range of colors or because of national and international regulations (10,11). ...
... Thus, styles of tattooing are often distinguished by adjectives describing the places they originated, such as 'Tahitian' or 'Japanese' " (2005, p. 19). In Bolivia, however, most tattoo artists describe the styles they tattoo as traditional/ old school American [tradicional, tradi, old school], neo-traditional [neo-tradicional, neo-tradi] Though some of the oldest tattoos in the world have been found in the Andes among the Chinchorro civilization (Pabst et al., 2009;Allison, 1996), tattooing fell out of practice when the Spanish colonized the Inca. It did not resurface with any consistency in the Andes until the late-1800s, when tattooed sailors began docking in Chilean ports. ...
Reference: Inking Identity