Earl Taliercio's research while affiliated with Agricultural Research Service and other places

Publications (45)

Article
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Key message Software for high imputation accuracy in soybean was identified. Imputed dataset could significantly reduce the interval of genomic regions controlling traits, thus greatly improve the efficiency of candidate gene identification. Abstract Genotype imputation is a strategy to increase marker density of existing datasets without addition...
Article
Crop wild relatives (CWRs) have high levels of genetic diversity compared to their domesticated descendants. Soybean (Glycine max) has over 20 species of CWRs, most of which are in secondary and tertiary gene pools. Glycine soja, hereafter ‘soja,’ is the only wild relative in the primary gene pool, i.e., species that readily cross with soybean. Soj...
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The soybean aphid (Aphis glycines) is an important pest of soybean (Glycine max). Wild soybean (Glycine soja; also known as ‘soja’) has many useful traits, including some accessions with aphid resistance that may be incorporated into modern soybean cultivars. The objective of this work was to evaluate 76 selected soja accessions and 14 associated s...
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Composition of fatty acids (FAs) in soybean seed is important for the quality and uses of soybean oil. Using gas chromatography, we have measured soybean FAs profiles of 621 soybean accessions (maturity groups I through IV) grown in five different environments; Columbus, OH (2015), Wooster, OH (2014 and 2015), Plymouth, NC (2015), and Urbana, IL (2...
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Two seed storage proteins, glycinin and β-conglycinin, account for most of the proteins in soymeal, while a valuable source of protein, soybean, has also been reported to be allergenic in some animals. Glycinin and conglycinin have been reported as allergens in multiple species, including humans. Allergic responses to soy proteins are due to hypers...
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USDA-Max × Soja Core Set-1 (USDA-MxS-CS1-1 to USDA-MxS-CS1-17 [Reg. No. GP-417 to GP-433, PI 689053 to PI 689069]) is a group of 17 interspecific breeding lines developed from the hybridization of lodging-resistant soybean cultivar N7103 [Glycine max (L.) Merr.] with wild soybean plant introduction PI 366122 [G. soja Siebold & Zucc.]. These materia...
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The relatively low genetic variation of current US soybean [Glycine max (L.) Merr.] cultivars constrains the improvement of grain yield and other agronomic and seed composition traits. Recently, a substantial effort has been undertaken to introduce novel genetic diversity present in wild soybean (Glycine soja Siebold & Zucc.) into elite cultivars,...
Article
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Background Ozone is an air pollutant widely known to cause a decrease in productivity in many plant species, including soybean (Glycine max (L.) Merr). While the response of cultivated soybean to ozone has been studied, very little information is available regarding the ozone response of its wild relatives. Results Ozone-resistant wild soybean acc...
Data
Allele specific bias of soybean gene models. (DOCX)
Data
Raw data used to calculate agronomic information. (XLSX)
Article
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Soybean [Glycine max (L.) Merr.] genetic diversity is limited because domesticated soybean has undergone multiple genetic bottlenecks. Its progenitor, the wild soybean [Glycine soja Siebold & Zucc], has not undergone the same intense selection and is much more genetically diverse than domesticated soybean. However, the agronomic importance of diver...
Article
Soybean genotype PI 416937 has previously been found to have low leaf hydraulic conductivity, which was hypothesized to be the basis for limited transpiration (TRlim) rates under high vapor pressure deficit. Previous studies with de-rooted shoots showed there was a consistency in the expression of TRlim and plant response to exposure to silver, an...
Article
Limiting the transpiration rate (TR) of a plant under high vapor pressure deficit (VPD) has the potential to improve crop yield under drought conditions. The effects of elevated VPD on the expression of genes in the leaves of three soybean accessions, Plant Introduction (PI) 416937, PI 471938 and Hutcheson (PI 518664) were investigated because thes...
Article
Vapour pressure deficit (VPD) is considered an important environmental factor that might affect leaf expansion and transpiration rate (TR) in plants. Two slow-wilting soybean (Glycine max (L.) Merr.) genotypes PI 416937 and PI 471938 along with commercial cultivar Hutcheson were subjected to low (1.2-1.6 kPa) and high VPD (2.8-3 kPa) environments t...
Article
β-conglycinin (conglycinin) is one of the major seed storage proteins of soybean. Conglycinin is a 7S trimer composed of different combinations of β, α and α' subunits. All of the subunits of conglycinin have been reported to be allergenic in humans. The goal of this research is to identify epitopes of the β-subunit of conglycinin that are antigeni...
Article
Soybean meal is commonly added to a variety of animal feeds to supplement protein sources and to optimise growth. While soybean protein is a valuable food supplement it has been recognised as an important food allergen. The soybean seed storage protein, glycinin, has been identified as an allergen. A tiled peptide array of the A1aBx and A5A4B3 subu...
Article
Background: Glycinin is a seed storage protein in soybean (Glycine max) that is allergenic in pigs. Glycinin is a hexamer composed of subunits consisting of basic and acidic portions joined by disulfide bridges. There are five glycinin subunit isoforms designated Gy1-Gy5. The purpose of this study is to identify epitopes from selected glycinin sub...
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While inositol has key roles in phytic acid and raffinosaccharide synthesis, its concentration in developing seed of low phytic acid soybean [Glycine max (L.) Merr.] lines derived from CX1834 (Wilcox et al., 2000) has not been compared to that of normal lines. Concentrations of metabolites in the phytic acid and raffinosaccharide biosynthesis have...
Article
Two cotton vacuolar-invertase genes were identified and sequenced. Both genes had seven exons, including an unusually small second exon typical of acid invertases. These genes encode peptides with many features shared by acid invertases from other species including, leader sequences that probably target the peptide to the vacuole, active site motif...
Article
ADP-glucose pyrophosphorylase (ADPGp, EC 2.7.7.27) is a tetrameric protein composed of two small and two large subunits that catalyzes the biosynthesis of ADP-glucose from glucose-phosphate which is used to provide the glucose subunits for starch biosynthesis. A second cotton gene encoding an ADPGp small subunit has been cloned and characterized. T...
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‘N6202’ soybean [Glycine max (L.) Merr.] (Reg. No. GP-366, PI 658498) was cooperatively developed and released by the USDA–ARS and the North Carolina Agricultural Research Service in October 2009 as a mid–Maturity Group VI germplasm with high-protein seed, favorable yield potential, large seed size, and diverse pedigree. The unusual combination of...
Article
Cotton fiber cells elongate without dividing to form economically valuable spinnable fiber. Reports of the ploidy level of fiber cells are variable. Early reports indicated an increase in nuclear DNA content in young fibers; however, subsequent reports failed to observe such a significant increase in ploidy level. Evaluation and analysis of genes i...
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Cotton (Gossypium hirsutum L) is an important crop worldwide that provides fiber for the textile industry. Cotton is a perennial plant that stores starch in stems and roots to provide carbohydrates for growth in subsequent seasons. Domesticated cotton makes these reserves available to developing seeds which impacts seed yield. The goals of these an...
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'N8101' soybean [Glycine max (L.) Merr.] (Reg. No. CV-498, PI 654355) was cooperatively developed and released by the USDA-ARS and the North Carolina Agricultural Research Service in February 2008 as a small-seeded Maturity Group VIII conventional cultivar. N8101 is the first publicly released small-seeded soybean cultivar in its maturity group and...
Article
Benzoquinone reductase (BR; EC 1.6.5.7) is an enzyme which catalyzes the bivalent redox reactions of quinones without the production of free radical intermediates. Using 2D-PAGE comparisons, two proteins were found to be up-regulated in wild-type cotton ovules during the fiber initiation stage but not in the fiberless line SL 1-7-1. These proteins...
Data
Gene expression in fiber initials. These data show expression and tentative identification of the sequences represented on the microarray.
Article
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Cotton (Gossypium hirsutum L.) fibers are trichomes that initiate from the ovule epidermis. Little is known about the developmental pathway causing fiber to differentiate from ovular epidermal cells even though limits on the number of cells that differentiate into fiber will limit yield. A method was developed to isolate RNA from fiber initials 1 d...
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Broadening the genetic base of upland cotton (Gossypium hirsutum L.) is essential for continuous genetic improvement of yield and fiber quality through breeding. The objectives of this study were to evaluate a species polycross (SP) population for phenotypic and genotypic variations in yield and fiber quality, investigate morphological variations a...
Article
A genomic clone representing a putative retinoblastoma binding (RBB) protein was isolated from a Gossypium hirsutum BAC library. Alignment of the gene sequence with the cDNA sequence indicated the gene consists of six exons that have standard eukaryotic splice junctions. The conceptual spliced transcript was 98% identical to TC37171 in the TIGR gen...
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In an effort to expand the Gossypium hirsutum L. (cotton) expressed sequence tag (EST) database, ESTs representing a variety of tissues and treatments were sequenced. Assembly of these sequences with ESTs already in the EST database (dbEST, GenBank) identified 9675 cotton sequences not present in GenBank. Statistical analysis of a subset of these E...
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Rhodamine B, a dye commonly used in a variety of biological studies was incorporated into the bodies of male tobacco budworm moths, Heliothis virescens (Lepidoptera: Noctuidae), by allowing them to feed freely on 0.1% rhodamine dissolved in a 10% sucrose solution. After exposing males for one to three days to this pigment, rhodamine was clearly det...
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Approximately 185,000 Gossypium EST sequences comprising >94,800,000 nucleotides were amassed from 30 cDNA libraries constructed from a variety of tissues and organs under a range of conditions, including drought stress and pathogen challenges. These libraries were derived from allopolyploid cotton (Gossypium hirsutum; A(T) and D(T) genomes) as wel...
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The cell cycle in cotton (Gossypium hirsutum) fibers is poorly understood. The objective of this study was to evaluate the cell cycle status and DNA content in developing cotton fibers. The DNA content and cell cycle distribution in fiber and hypocotyl cells were determined by flow cytometry. Expression levels of minichrosomal maintenance protein (...
Article
UDP-glucose is the primary substrate for cellulose synthase, the enzyme that produces the main chemical constituent of cotton fiber. Two enzymes, UDP-glucose pyrophosphorylase (UDPGp, EC 2.7.7.9) and sucrose synthase (SuSy, EC 2.4.1.13) catalyze the synthesis of UDP-glucose. SuSy plays an important role in cellulose metabolism during rapid secondar...
Article
UDP-glucose is the primary substrate for cellulose synthase, the enzyme that produces the main chemical constituent of cotton fiber. Two enzymes, UDP-glucose pyrophosphorylase (UD- PGp, EC 2.7.7.9) and sucrose synthase (SuSy, EC 2.4.1.13) catalyze the synthesis of UDP-glucose. SuSy plays an important role in cellulose metabo- lism during rapid seco...
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Differential display has been widely and successfully used to identify differentially expressed genes based on physiological treatments or genetic variation. We used differential display to identify genes based on their site of translation. Identification was made based on the fractionation of RNA from soybean leaves into total RNA, free polyriboso...

Citations

... Faba bean and rapeseed/canola proteins are newcomers that are raising interest ( Boukid and Castellari, 2022 ). Glycinin and -conglycinin are considered the major allergens of soy proteins with around 42 identified epi-topes ( Taliercio and Loveless, 2021 ;Zhao et al., 2021 ). Soy allergies can lead skin reactions and gastrointestinal symptoms ranging from mild to severe (enterocolitis atopic eczema and immediate IgE -mediated reactions) ( Labrosse et al., 2020 ;Onyimba et al., 2021 ). ...
... Additionally, SNPs can be categorized as either transitions (C/T or G/A) or transversions (C/G, A/T, C/A, or T/G), according to the nucleotide substitution. Moreover, SNP markers have been identified in numerous crop species, such as rice [13], maize [14], wheat [15], and soybean [16]. A few thousand markers are sufficient for quantitative trait locus mapping. ...
... Due to limited seed stocks, the no-choice tests were performed once for each colony. In addition, a nonparametric, marginal effect ANOVA-type test was performed to determine whether infestations ratings differed by accession Phytoparasitica (Hesler et al. 2019). When rankings varied significantly (α = 0.05) by accession, the LSMEANS option was used to separate mean rankings among accessions. ...
... In order to advance the soybean industry, the sustainable utilization of wild soybean resources will provide the foundation for soybean innovation and production in this area. The comprehensive evaluation of agronomic traits of wild soybean will enrich the genetic basis of soybean cultivation, make breakthrough in soybean breeding, enhance the ability of soybean stress resistance, ensure the sustainable development of the soybean industry, and protect and effective use of wild soybean resources [10][11][12]. In this review, we investigated the agronomic traits of 242 wild soybean samples, providing a strong foundation for future application of wild soybeans, to improve the quality of cultivated soybeans. ...
... Unfortunately, crushed soybean seeds cause unpleasant grassy and off-flavors especially in the production of soybean-based beverages which ultimately reduce the soybean-derived product quality (Mellor et al., 2010). In addition, the soybean-derived product soy meal, which is considered a valuable source of protein, also contains allergenic properties (Taliercio and Loveless, 2020). Therefore, regulating undesirable characteristics like offflavor and, allergenic properties of soy protein is also a big challenge for soybean quality improvement strategies. ...
... Thus, improving seed yield and germination is the major breeding target while developing low phytate grain crops. High and low phytic acid maize populations isolated after three cycles of recurrent selection differed significantly for phytic acid To date a few low seed phytate cultivars such as barley 'Herald', 'Clearwater', and 'Sawtooth' [64][65][66] and soybean 'USDA-N6003LP' and 'N6202' [67,68] have been released for cultivation in USA. These cultivars were developed by crossbreeding, and combine moderate reduction in seed phytate, multiple fold increase in Pi, adaptation, productivity (yielding at par or up to 90% of the control), seed quality, and good field emergence. ...
... The utility of wild soybean in soybean breeding is well understood and continues to help breeders to improve cultivated soybean. For example, recently, 17 agronomic interspecific (USDA-Max 9 Soja core set-1) germplasm lines were released (Eickholt et al. 2019). Similarly, genome-wide association analyses of yield and domestication related traits has helped in the discovery of important genes (Hu et al. 2020;La et al. 2019;Prince et al. 2020). ...
... Diers et al. (1992) reported similar heritability for oil at 0.92 and protein at 0.74. A core collection evaluation of G. max and G. soja seed composition reported 36-40% for G. max check lines and 39-48% for G. soja accessions for protein concentration and a variation of 21-25% for G. max check lines and 15-17% for G. soja accessions for oil concentration (La et al., 2019). Based on this and other studies, G. soja accessions tend to have more protein content and less oil content than G. max lines. ...
... Pisum sativum, Glycine max and Phaseolus vulgaris (Yendrek et al., 2015). Glycine max has also been extensively studied for transcriptome annotation under ozone stress for leaf tissue, seed coat, flower and pod (Leisner et al., 2014;Whaley et al., 2015;Leisner et al., 2017;Waldeck et al., 2017). Transcriptome studies have also been done on Medicago truncatula and Pak choi using DNA microarray and RNA-seq respectively (Iyer et al., 2013;Zhang et al., 2017). ...
... Insufficient soybean production in China and many developing countries is a big challenge; yield of soybean needs to be improved rapidly [24]. Existing studies have demonstrated significant yield of soybean hybrids over their parents [25][26][27][28], but our knowledge of soybean heterosis is very superficial; in addition, the effects of epigenetic mechanisms have rarely been reported. In this work, we investigated DNA methylation differences between three reciprocal hybrids and their parental lines in the middle developmental period of contemporary seed by MethylRAD-seq (methylation-dependent restriction-site associated DNA sequencing) that can identify CCGG and CCWGG (where W = A or T) methylated sites in the whole genome [29]. ...