July 1998
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1,375 Reads
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1 Citation
Resonance
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July 1998
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1,375 Reads
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1 Citation
Resonance
January 1989
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135 Reads
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31 Citations
January 1989
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67 Reads
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115 Citations
January 1989
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123 Reads
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67 Citations
January 1989
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56 Reads
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94 Citations
January 1989
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41 Reads
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28 Citations
January 1989
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67 Reads
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30 Citations
January 1989
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85 Reads
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3 Citations
January 1989
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154 Reads
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606 Citations
January 1989
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21 Reads
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11 Citations
... Parentage analysis was performed on the foal produced by SCNT to confirm genetic identity with the somatic donor cells. The DNA from blood and cells was extracted by the standard proteinase K/phenol-chloroform procedure [21]. The extracted genomic DNA samples were subjected to microsatellite assay using a 17-plex Applied Biosystems (USA) horse genotyping kit (VHL20, HTG4, AHT4, HMS7, HTG6, HMS6, HTG7, HMS3, AHT5, ASB2, HTG10, HMS2, ASB17, LEX3, HMS1, CA425, and ASB23) labeled with one of the fluorescent dyes, FAM, NED, PET or VIC. ...
January 1989
... M formaldehyde gel. (26) Even loading of the RNA was verified by ethidium bromide staining. After electrophoresis, the gel was washed in deionized water to remove excess formaldehyde. ...
January 1989
... While the overall number of MKRH alive today is unknown, it is estimated to be less than 100. DNA was isolated using a proteinase K-SDS chloroform phenol extraction protocol (Sambrook et al. 1989). A previously reported dataset of 1346 dogs genotyped at 150 112 single-nucleotide polymorphisms (SNPs) using the Canine HD Whole-Genome Genotyping BeadChip (173 662 total SNPs) from Illumina (San Diego, CA) was utilized (Parker et al. 2017). ...
January 1989
... Agarose gel was visualized using a gel documentation system. The DNA ladder from (SolGent™ 1 Kb Plus DNA Ladder) was used as a molecular mass marker and the fragment size of approximately 886 bp and 527bp was verified as positive for 16SrDNA gene of Staphylococcus sp., and mecup gene, respectively (Sambrook et al., 1989). ...
... We also compared the Y haplogroup frequencies estimated from the STR haplotypes of 255 individuals from the State of Rio Grande do Sul in southern Brazil (Schwengber et al., 2009), 200 individuals from the State of São Paulo in southeastern Brazil (Góis et al., 2007), 200 individuals from the city of Belém in the State of Pará, northern Brazil (Palha et al., 2007) and 48 individuals from the city of Brasília, Federal District in central-western Brazil (Grattapaglia et al., 2005). DNA was extracted from peripheral blood mononuclear cells using the phenol-chloroform procedure (Sambrook et al., 1989) and was quantified spectrophotometrically (NANODROP 1000 spectrophotometer, Thermo Scientific, Wilmington, DE, USA). Polymerase chain reactions (PCR) were done with 1-2 ng of template DNA using commercial kits, according to the manufacturer's instructions (AmpFLSTR ® Y-filer PCR amplification kit, Applied Biosystems). ...
January 1989
... (U.S.A.). Cytological preparations, aged 2-7 days at 4°C, were incubated at 37°C for 16 hrs in 100 j.d of the enzyme solution (10-20 units of one enzyme in a Tris-saline buffer (Maniatis et a!., 1982)). The enzyme solution was evenly dispersed on the preparations by use of a coverslip. ...
January 1982
... Cultures were maintained overnight at 37°C in a humidified atmosphere of 5% CO 2 . Non-adherent cells were removed by washing with RPMI medium, and adherent cells were digested with lysis buffer 18 and stored at −80°C for PCR analysis. ...
January 1989
... Total cellular RNA was separated by electrophoresis on horizontal 1% agarose gels containing 2.2 M formaldehyde and transferred to nylon filters (NEN Research Products, Boston, MA, USA) (22). Quantity, integrity and efficiency of RNAs transfer were checked by ethidium bromide staining of the 18s and 28s ribosomal RNA bands before and after transferring the RNA from the gels to membranes. ...
Reference:
Miranda J Hep mdr2 1997
January 1989
... RNases are present in all cells and found in most secretions/excretions from living organisms. For that reason, RNases are ubiquitous in the laboratory environment, i.e., on human skin, laboratory glassware, metalware, and in laboratory working solutions [11][12][13][14]. RNases are heat-tolerant, stable over a wide range of pH, and resistant to many denaturing agents [15,16]. ...
January 1989
... RBCs were separated from plasma using low-speed centrifugation (1,000 g) and washed twice in ice-cold 120 mM NaCl before addition of lysis buffer, while other Amphiuma tissues were homogenized with a polytron. Poly(A) ϩ RNA was isolated from total cellular RNA following two successive enrichments using oligo(dT)-cellulose (39). ...
January 1989