Drew Kuraitis's research while affiliated with Tulane University and other places

Publications (63)

Article
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The field of macrophage biology is rapidly growing. Recent studies have shifted focus from classic wound healing roles to newly identified roles in dermatologic pathology. These studies have identified pathogenic roles of macrophages in relatively common conditions, such as psoriasis, skin cancer, and cutaneous T-cell lymphoma. Selective depletion...
Article
Background: Surgical excision is the paradigm treatment option for non-melanoma skin cancer (NMSC), however intralesional fluorouracil (IL 5-FU) is an efficacious alternative and superior to other chemotherapy agents in NMSC. Yet, little summative data exists on the topic. Objective: To assess the efficacy of IL 5-FU in the treatment of NMSC. M...
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Physician gender may impact their exposure to genital dermatoses during residency. The purpose of this study was to survey current dermatology residents regarding their comfort in diagnosing and managing lichen sclerosus. As residents progress through training, confidence improves in diagnosing and managing both male and female lichen sclerosus. Ho...
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We present a 32-year old woman with a 9-year history of upper facial swelling. A workup by the ophthalmology department led to the diagnosis of Melkersson-Rosenthal syndrome. Re-evaluation in our dermatology clinic confirmed a diagnosis of Morbihan disease. Herein, we review case reports and case series of upper facial swelling in the dermatologic...
Article
Coronary artery disease is a leading cause of death in developed nations. As the disease progresses, myocardial infarction can occur leaving areas of dead tissue in the heart. To compensate, the body initiates its own repair/regenerative response in an attempt to restore function to the heart. These efforts serve as inspiration to researchers who a...
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The bacterium Staphylococcus aureus is responsible for significant morbidity, mortality, and financial burden in healthcare. It easily colonizes susceptible patients and can cause recurrent infections, especially in populations at risk. In addition to treating sequelae of infections, there is a growing body of literature aimed at decolonizing susce...
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The use of biomaterials and tracking the long-term fate of the transplanted cells is expected to help improve the clinical translation of cell therapies for cardiac regeneration. To this end, reporter gene strategies are promising for monitoring the fate of cells transplanted with or without a delivery biomaterial; however, their application with p...
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Injectable hydrogel biomaterials are promising therapies to promote repair and regeneration post-myocardial infarction (MI). However, the timing of delivery and the mechanisms through which biomaterial treatments confer their benefits are translational issues that remain to be addressed. We assessed the efficacy of an injectable collagen matrix at...
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Background: Injectable hydrogel biomaterials have emerged as promising therapies for treating myocardial infarction (MI). We developed a collagen type I based injectable hydrogel matrix that can prevent the deterioration of cardiac function when delivered soon post-MI and found that the effects may be mediated through a microRNA (miRNA) mechanism....
Article
The extracellular matrix (ECM) is an essential feature of development, tissue homeostasis and recovery from injury. How the ECM responds dynamically to cellular and soluble components to support the faithful repair of damaged tissues in some animals but leads to the formation of acellular fibrotic scar tissue in others has important clinical implic...
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Injectable delivery matrices hold promise in enhancing engraftment and the overall efficacy of cardiac cell therapies; however, the mechanisms responsible remain largely unknown. Here we studied the interaction of a collagen matrix with circulating angiogenic cells (CACs) in a mouse myocardial infarction model. CACs + matrix treatment enhanced CAC...
Article
Ischemic heart disease persists as the leading cause of death in the western world. The goal of cell therapy is to supply the ischemic heart with therapeutic cells that are able to restore vasculature and cardiomyocytes, or at least to an extent that improves heart function and patient quality-of-life. This chapter will discuss the potential of cel...
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Methylglyoxal accumulates in diabetes and impairs neovascularization. This study assessed whether over-expressing the methylglyoxal-metabolizing enzyme glyoxalase-1 (GLO1) in only bone marrow cells (BMCs) could restore neovascularization in ischemic tissue of streptozotocin-induced diabetic mice.Methods and ResultsAfter 24 h of hyperglycemic and hy...
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-Vasculogenic cell-based therapy combined with tissue engineering is a promising revascularization approach destined at patients with advanced coronary artery disease, many of whom exhibit myocardial hibernation. However, so far no experimental data have been available in this context; we therefore examined the biopolymer-supported delivery of circ...
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Blood-derived circulatory angiogenic cells (CACs) and resident cardiac stem cells (CSCs) have both been shown to improve cardiac function after myocardial infarction. The superiority of either cell type has long been an area of speculation with no definitive head-to-head trial. In this study, we compared the effect of human CACs and CSCs, alone or...
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Biomaterials that are similar to skeletal muscle extracellular matrix have been shown to augment regeneration in ischemic muscle. In this study, treatment with a collagen-based matrix stimulated molecular myogenesis in an mdx murine model of necrosis. Matrix-treated animals ran ≥40% further, demonstrating functional regeneration, and expressed incr...
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Introduction: Coronary artery disease commonly leads to myocardial ischaemia and hibernation. Relevant preclinical models of these conditions are essential to evaluate new therapeutic options such as cell-based vasculogenic therapies. Areas covered: In this article, the authors first review basic concepts of myocardial ischaemia/hibernation and...
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Cell transplantation for regenerative medicine has become an appealing therapeutic method; however, stem and progenitor cells are not always freshly available. Cryopreservation offers a way to freeze cells as they are generated, for storage and transport until required for therapy. This study was performed to assess the feasibility of cryopreservin...
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Biomaterial-guided regeneration represents a novel approach for the treatment of myopathies. Revascularisation and the intramuscular extracellular matrix are important factors in stimulating myogenesis and regenerating muscle damaged by ischaemia. In this study, we used an injectable collagen matrix, enhanced with sialyl LewisX (sLeX), to guide ske...
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Cell therapy is expected to restore perfusion and improve function in the ischemic/infarcted myocardium; however, the biological mechanisms and local effects of transplanted cells remain unclear. To assess cell fate in vivo, hexadecyl-4-[¹⁸F]fluorobenzoate (¹⁸F-HFB) cell labeling was evaluated for tracking human circulating progenitor cells (CPCs)...
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Myopathies of skeletal muscle are prevalent diseases worldwide. To address this, regenerative therapies are being developed to restore perfusion to ischemic muscle and to reverse muscle wasting. There are adult stem cell populations that inherently possess these therapeutic properties; however, cell transplantation trials in the clinic have shown m...
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Potential benefits of co-culturing monocytes (MC) with vascular smooth muscle cells have been reported on for tissue engineering applications with a degradable, polar, hydrophobic, and ionic polyurethane (D-PHI). Since the interaction of MC and endothelial cells (EC) within the blood vessel endothelium is also a process of wound repair it was of in...
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Although many regenerative cell therapies are being developed to replace or regenerate ischaemic muscle, the lack of vasculature and poor persistence of the therapeutic cells represent major limiting factors to successful tissue restoration. In response to ischaemia, stromal cell-derived factor-1 (SDF-1) is up-regulated by the affected tissue to st...
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Injectable hydrogels are increasingly being developed for biomedical applications due to their ability to be delivered in a minimally invasive manner. One potential use for such materials is in cell delivery for cardiac regeneration. While the materials' properties are often characterized, how these properties (and in particular gelation) are affec...
Article
Despite recent studies suggesting that the heart has instrinsic mechanisms of self-regeneration following myocardial infarction, it cannot regenerate itself to an optimal level. Mesenchymal stem cells (MSCs) are currently being investigated for regeneration of mesenchyme-derived tissues, such as bone, cartilage and tendon. In vitro evidence suggest...
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Biomaterials that have the ability to augment angiogenesis are highly sought-after for applications in regenerative medicine, particularly for revascularization of ischemic and infarcted tissue. We evaluated the culture of human circulating angiogenic cells (CAC) on collagen type I-based matrices, and compared this to traditional selective-adhesion...
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We sought to identify an essential component of the TEAD4/VGLL4 transcription factor complex that controls vascular endothelial growth factor A (VEGFA) expression in muscle. A yeast 2-hybrid screen was used to clone a novel component of the TEAD4 complex from a human heart cDNA library. We identified interferon response factor 2 binding protein 2 (...
Article
Stem cell therapy for cardiac disease may be facing two major problems nowadays: although vasculogenesis likely occurs as a result of cell therapy, its clinical applications are limited and significant, integrated cardiomyogenesis has not demonstratively been shown to occur, even in the experimental setting, with any other source than embryonic or...
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Cell therapy for the treatment of cardiovascular disease has been hindered by low cell engraftment, poor survival, and inadequate phenotype and function. In this study, we added chitosan to a previously developed injectable collagen matrix, with the aim of improving its properties for cell therapy and neovascularization. Different ratios of collage...
Article
Circulating progenitor cells home to and engraft to sites of ischemia, mediated in part by the adhesion molecule L-selectin; however, accumulation in tissues such as the heart is low. In this study, an acellular collagen-based matrix containing sialyl Lewis(X) (sLe(X)), which binds L-selectin, was developed in order to enhance the endogenous progen...
Article
Cardiac cell therapy has not yet resulted in long-term clinical benefits or major recovery of myocardial function in humans. To date, most of the cardiac effects of cell-based therapy are believed to be mediated by a local angiogenic response rather than by the formation of neosyncytial contractile units such as had initially been hoped for. Theref...

Citations

... Murine studies demonstrated that depletion of macrophages improved psoriasis inflammation (87)(88)(89) and reduced the levels of Th1 cytokines, including IL-1a, IL-6, IL-23 and TNFa to normal levels (90). These results underscore that macrophages contribute to the development and maintenance of psoriatic lesions (86). Psoriasis patients have an increased level of circulating monocytes in peripheral blood (91,92), and they favored the M1 phenotype (93). ...
... Es un antimetabolito que inhibe la timidilato sintasa, interfiriendo en la síntesis del ADN y en la división celular. El 5-FU intralesional ha sido utilizado con un alto porcentaje de respuestas completas en el tratamiento del CCNM 3,4 . A continuación, presentamos una paciente añosa con un carcinoma escamoso cutáneo (CEC) con resolución completa tras el tratamiento con 5-FU intralesional. ...
... [17,18] 5-FU is a pyrimidine analogue that exerts a potent antimitotic activity and has been widely used in the treatment of actinic keratoses, as well as some basal and squamous cell skin cancers. [19] However, relatively few studies have focused on the extra-genotoxic actions of this chemotherapeutic agent. A previous study by Tsuji et al [20] demonstrated that 5-FU exerts a selective cytotoxicity according to the type of epidermal cells. ...
... Moreover, when immunohistochemical analysis was added, 61 of 67 patients (91%) met the criteria for the diagnosis of early MF. Similarly, the other group also showed that the sensitivity of the algorithm excluding biological molecular criteria was 93%, while the algorithm including the criteria achieved 100% sensitivity [31]. Collectively, the algorithm is highly sensitive and most early MF cases can be diagnosed accurately, but the specificity has not yet been validated sufficiently. ...
... Nevertheless, the interplay among genetics, lifestyle, hormonal status, microbiome, and innate and adaptive immune system remains unclear. Furthermore, multicenter and large-scale (racially diverse [265]) OMICs studies are needed to identify novel actors (and possible new therapeutic targets) in HS etiopathogenesis and to improve tailored therapy [266]. In the near future, artificial intelligence (AI) might play a role in HS, firstly in clinical trials, and then becoming useful (for clinicians and patients) in daily clinical practice [267,268]. ...
... Cyclophosphamide Bone marrow suppression [43] Hydroxychloroquine Bone marrow suppression and hemolysis (rare; primarily a theoretical concern in patients with glucose-6-phosphate deficiency) [44] Mycophenolate Bone marrow suppression (reversible) [45] Azathioprine Bone marrow suppression (dose-dependent) [46] NSAIDs Gastrointestinal blood loss with iron deficiency [47] ...
... Induction of interferon regulatory factor 1 (IRF1) plays a key role in the activation of the inflammatory response to LPS (7) by binding to cis-regulatory DNA sequences of interferon-responsive genes. Under basal conditions, these interferon-responsive genes are suppressed by competitive binding of the related factor IRF2 that is constitutively expressed (8). IRF2 owes its repressor function to its interaction with IRF2 binding protein 2 (IRF2BP2) (9) that recruits the corepressors NCOR1 (10) and VGLL4 (11). ...
... Despite the benefits and promising results of the biomaterial-based hydrogel therapies for heart disease in preclinical models, a number of obstacles and limitations still hinder their clinical translation. One disadvantage of the biomaterials, in particular natural ones, is that they may retain some surface antigens to exert unignorable foreign body reaction in the host [64,118,119]. As controlling with this immune response is essential for successful functionality and sustainability of implanted hydrogels, there are a lot of attempts to develop biomaterials that can modulate these immunologic reactions, which involve functionalization with appropriate bioactive molecules of immunomodulatory factors (e.g., interleukin-10 [IL-10], tissue inhibitor of metalloproteinase-3 [TIMP-3]) [120][121][122]. ...
... S. aureus has long been recognized as an important player in the pathogenesis of AD; therefore, decolonization of S. aureus is considered essential in AD treatment [23]. Our results revealed that systemic administration of ASCs not only prevented apoptosis of PBMCs, but also enhanced the phagocytic activity of PBMCs in AD rats. ...
... Cells were seeded on fibronectin-coated plates (20 μg/plate) in EBM-2 (Clonetics) supplemented with EGM-2-MV SingleQuots (Clonetics). After 4 days in culture, an adherent heterogeneous CAC population was generated, which was previously characterized (Sofrenovic et al., 2012). CACs were lifted from the plates using PBS, and count and viability were assessed using a Vi-Cell counter (Beckman Coulter) via the Trypan blue exclusion method, prior to embedding them in matrices. ...