Dijon Henrique Salomé de Campos's research while affiliated with São Paulo State University and other places

Publications (56)

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Purpose This study aimed to evaluate the effect of rice bran (RB) supplementation to a high-sugar fat (HSF) diet on cardiac dysfunction in an experimental obesity model. Methods Male Wistar rats were distributed into three groups: control, high-sugar fat, and high-sugar fat supplemented with 11% RB for 20 weeks. Results HSF diet promoted obesity...
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Background: Maladaptive cardiac remodelling is characterized by diastolic and systolic dysfunction, culminating in heart failure. In this context, the dysfunctional scenario of cardiac calcium (Ca2+) handling has been poorly studied. An experimental model of aortic stenosis has been extensively used to improve knowledge about the key mechanisms of...
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Mechanisms involved in cardiac function and calcium (Ca2+) handling in obese-resistant (OR) rats are still poorly determined. We tested the hypothesis that unsaturated high-fat diet (HFD) promotes myocardial dysfunction in OR rats, which it is related to Ca2+ handling. In addition, we questioned whether exercise training (ET) becomes a therapeutic...
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Background: As some individuals present resistance to obesity development, experiments have been trying to understand their susceptibility to cardiometabolic diseases. Objective: To evaluate if the cardiac remodeling was related to collagen protein expression change. Methods: Male Wistar rats were randomized into two experimental groups: control di...
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Aim To evaluate the influence of physical training on myocardial function, oxidative stress, energy metabolism, and MAPKs and NF-κB signaling pathways in spontaneously hypertensive rats (SHR), at advanced stage of arterial hypertension, which precedes heart failure development. Methods We studied four experimental groups: normotensive Wistar rats...
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Metabolic syndrome (MetS) include obesity as a critical feature and is strongly associated with risk of cardiovascular disease (CVD). Insights into mechanisms involved in the pathophysiology of these clinical manifestations are essential for the development of therapeutic strategies. Thus, Western diets (WD) have been widely employed in diet-induce...
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The aim of this study is to evaluate whether the alterations in glucose metabolism and insulin resistance are mechanisms presented in cardiac remodelling induced by the toxicity of cigarette smoke. Male Wistar rats were assigned to the control group (C; n = 12) and the cigarette smoke‐exposed group (exposed to cigarette smoke over 2 months) (CS; n...
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Background/aims: The beneficial effect of aerobic exercise training (ET) on cardiac remodeling caused by supravalvar aortic stenosis (AS) has been demonstrated in experimental studies; however, the mechanisms responsible for improving cardiac function are not entirely understood. We evaluated whether ET-generated cardioprotection in pressure-overl...
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Background/aims: Aortic stenosis-induced chronic pressure overload leads to cardiac dysfunction and congestive heart failure. The pathophysiological mechanisms of the myocardial impairment are multifactorial and include maladaptive β-adrenergic signaling. Exercise training (ET) has been used as a non-pharmacological therapy for heart failure manag...
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Fundamento A obesidade tem sido associada com ativação crônica do sistema renina-angiotensina-aldosterona e importantes alterações no desempenho cardíaco. Objetivo Avaliar a influência do bloqueio de receptores de angiotensina-II do tipo 1 (AT1) sobre a morfologia e desempenho cardíaco de ratos obesos por dieta Métodos Ratos Wistar (n=48) foram s...
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Background: Obesity is a risk factor for medical complications, including the cardiovascular system. There is limited information on collagen in the heart in obesity. Our previous study showed decreased protein levels of myocardial collagen type I in obese rats fed a high-fat diet for 34 weeks. However, the mechanisms responsible for low levels are...
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Obesity is a pandemic associated with a high incidence of cardiovascular disease; however, the mechanisms are not fully elucidated. Proteomics may provide a more in-depth understanding of the pathophysiological mechanisms and contribute to the identification of potential therapeutic targets. Thus, our study evaluated myocardial protein expression i...
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Background Interstitial collagen matrix increase and left ventricular (LV) diastolic dysfunction are the most frequent cardiac alterations during aging. Whether changes are part of the aging process or induced by a sedentary life style is not clear. Purpose To evaluate the effects of aerobic exercise on functional capacity, cardiac and myocardial...
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Introduction Physical exercise reduces systemic arterial blood pressure and improves cardiac remodeling. However, the effects of exercise during uncontrolled arterial hypertension remains poorly understood. This study evaluated the influence of physical training on cardiac remodeling in untreated spontaneously hypertensive rats (SHR). Methods Four...
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Severe food restriction (FR) impairs cardiac performance, although the causative mechanisms remain elusive. Since proteins associated with calcium handling may contribute to cardiac dysfunction, this study aimed to evaluate whether severe FR results in alterations in the expression and activity of Ca2+-handling proteins that contribute to impaired...
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Background: The higher consumption of fat and sugar are associated with obesity development and its related diseases such as non-alcoholic fatty liver disease (NAFLD). Lycopene is an antioxidant whose protective potential on fatty liver degeneration has been investigated. The aim of this study was to present the therapeutic effects of lycopene on N...
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Obesity is often associated with changes in cardiac function; however, the mechanisms responsible for functional abnormalities have not yet been fully clarified. Considering the lack of information regarding high-saturated-fat diet-induced obesity, heart function, and the proteins involved in myocardial calcium (Ca2+) handling, the aim of this stud...
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Palatable hypercaloric feeding has been associated with angiotensin‐II type 1 receptor (AT1R) stimulation and cardiac remodeling. This study analyzed whether AT1R antagonism attenuates cardiac remodeling in rats subjected to a palatable hypercaloric diet. Male Wistar‐Kyoto rats were subjected to a commercial standard rat chow (CD) or a palatable hy...
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Cardiac remodeling (CR) is a structural change of the heart due to chronic hemodynamic overload related to changes in both myocyte and extracellular matrix (ECM). We investigated that the imbalance of collagen V promotes cardiomyocyte apoptosis that contributes to heart failure and cell death. Aortic stenosis was induced surgically and male Wistar...
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Background Increased reactive oxygen species (ROS) generation in diabetes mellitus (DM) is an important mechanism leading to diabetic cardiomyopathy. Apocynin, a drug isolated from the herb Picrorhiza kurroa, is considered an antioxidant agent by inhibiting NADPH oxidase activity and improving ROS scavenging. This study analyzed the influence of ap...
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The aim of this study is to test the hypothesis that obesity induced by a diet rich in saturated fats and balanced in carbohydrates is associated with the development of systemic complications and comorbidities. Thirty-seven 60-day-old male Wistar rats were randomized into two groups: control (C, n = 18, standard diet) and obese (OB, n = 19, high-s...
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Background: The high consumption of fat and sugar contributes to the development of obesity and co-morbidities, such as diabetes, and cardiovascular and kidney diseases. Different strategies have been used to prevent these diseases associated with obesity, such as changes in eating habits and/or the addition of dietary components with anti-inflamm...
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Background: Different types of high-fat and/or high-energy diets have been used to induce obesity in rodents. However, few studies have reported on the effects observed at the initial stage of obesity induced by high-fat feeding on cardiac functional and structural remodelling. Objective: To characterize the initial moment of obesity and investiga...
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Authors have showed that obesity implicates cardiac dysfunction associated with myocardial L-type calcium channels (LTCCs) activity impairments, as well as moderate exercise training (MET) seems to be an important therapeutic tool. We tested the hypothesis that MET promotes improvements on LTCCS activity and protein expression at obesity induced by...
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Cardiac remodeling is defined as changes in shape and function of the heart in response to aggression (pressure overload). The sarcoplasmic reticulum calcium ATPase cardiac isoform 2a (SERCA2a) is a known factor that influences function. A wide spectrum of studies report a decrease in SERCA2a in heart failure, but none evaluate it's the role in ear...
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Objective: The current study was aimed at analyzing sarcoplasmic reticulum Ca2+ ATPase (Serca2) and ryanodine receptor type 2 (Ryr2) gene expression in rats subjected to surgery that induced HF and were subsequently treated with T4 using physiological doses. Materials and methods: HF was induced in 18 male Wistar rats by clipping the ascending t...
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Obesity is a worldwide pandemic associated with high incidence of cardiovascular disease. The mechanisms by which the obesity leads cardiac dysfunction are not fully elucidated and few studies have evaluated the relationship between obesity and proteins involved in myocardial β-adrenergic (βA) system. The purpose of this study was to evaluate the c...
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PurposeAlthough increased oxidative stress is a major component of diabetic hypertensive cardiomyopathy, research into the effects of antioxidants on cardiac remodeling remains scarce. The actions of antioxidant apocynin include inhibiting reactive oxygen species (ROS) generation by nicotinamide adenine dinucleotide phosphate (NADPH) oxidases and R...
Article
Methods: Three days after inducing AS, weaning rats were randomized to receive spironolactone (AS-SPR, 20mg/kg/day) or no drug (AS) for 18weeks, and compared with sham-operated rats. Myocardial function was studied in isolated left ventricular (LV) papillary muscles. Statistical analyses: ANOVA or Kruskal-Wallis tests. Results: Echocardiogram...
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Introduction: Obesity is a disease in which inflammation is directly involved and can lead to impaired renal function. Objective: To evaluate the influence of short term exposure to cafeteria diet on kidney tissue inflammation and advanced glycation end products (AGEs) in the rat plasma. Methods: Male Wistar rats (10 weeks of age, weighing 350...
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In experimental studies, several parameters, such as body weight, body mass index, adiposity index, and dual-energy X-ray absorptiometry, have commonly been used to demonstrate increased adiposity and investigate the mechanisms underlying obesity and sedentary lifestyles. However, these investigations have not classified the degree of adiposity nor...
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Exercise training (ET) has beneficial effects on the myocardium in heart failure (HF) patients and in animal models of induced cardiac hypertrophy and failure. We hypothesized that if microRNAs (miRNAs) respond to changes following cardiac stress, then myocardial profiling of these miRNAs may reveal cardio-protective mechanisms of aerobic ET in HF....
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Obesity has been shown to impair myocardial performance. Some factors have been suggested as responsible for possible cardiac abnormalities in models of obesity, among them beta-adrenergic (βA) system, an important mechanism of regulation of myocardial contraction and relaxation. The objective of present study was to evaluate the involvement of βA...
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Physical exercise is a strategy to control hypertension and attenuate pressure overload-induced cardiac remodeling. The influence of exercise on cardiac remodeling during uncontrolled hypertension is not established. We evaluated the effects of a long-term low intensity aerobic exercise protocol on heart failure (HF) development and cardiac remodel...
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Background: Several researchers seek methods for the selection of homogeneous groups of animals in experimental studies, a fact justified because homogeneity is an indispensable prerequisite for casualization of treatments. The lack of robust methods that comply with statistical and biological principles is the reason why researchers use empirical...
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Background: Obesity is defined by excessive accumulation of body fat relative to lean tissue. Studies during the last few years indicate that cardiac function in obese animals may be preserved, increased or diminished. Objective: Study the energy balance of the myocardium with the hypothesis that the increase in fatty acid oxidation and reduced gl...
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Few studies have evaluated the relationship between the duration of obesity, cardiac function and the proteins involved in myocardial calcium (Ca(2+)) handling. We hypothesized that long-term obesity promotes cardiac dysfunction due to a reduction of expression and/or phosphorylation of myocardial Ca(2+)-handling proteins. Thirty-day-old male Wista...
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Obesity is a risk factor for many medical complications; medical research has shown that hemodynamic, morphological and functional abnormalities are correlated with the duration and severity of obesity. Present study determined the influence of term of exposure to high-fat diet-induced obesity on myocardial collagen type I and III. Thirty-day-old m...
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The aim of this study was to test the hypothesis that physical training (PT) attenuates cardiac remodeling and the severity of heart failure in rats with aortic stenosis. Wistar male rats were divided in sham (S, n=10), sham training (TR, n=4), aortic stenosis (AS, n=5) and aortic stenosis training (ASTR, n=5). Aortic constriction was created by pl...
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Cited By :1, Export Date: 21 July 2015, CODEN: ABCAA, Correspondence Address: Cordellini, S.; Departamento de Farmacologia, Instituto de Biociências-UNESP, Rua João Carmelo, 145 - Jardim Paraiso - 18610-290 - Botucatu, Sao Paulo, Brazil; email: bruderthiago@yahoo.com.br, Chemicals/CAS: arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; insulin, 9...
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Cited By :11, Export Date: 21 July 2015, Correspondence Address: Okoshi, M. P.; Departamento de Clínica Médica, Faculdade de Medicina de Botucatu, UNESP, Rubiao Junior, S/N, CEP: 18618-000, Botucatu, Sao Paulo, Brazil; email: mpoliti@fmb.unesp.br, Molecular Sequence Numbers: GENBANK: NM_013172, NM_017101, NM_017115, NM_176079;, Chemicals/CAS: myoge...
Article
Cited By :3, Export Date: 21 July 2015, CODEN: MSMOF, Correspondence Address: Okoshi, M. P.; Departamento de Clinica Medica, Faculdade de Medicina de Botucatu, UNESP, Rubiao Junior, S/N, CEP: 18618-000, Botucatu, Sao Paulo, Brazil; email: mpoliti@fmb.unesp.br, Chemicals/CAS: myogenin, 127385-49-3; Interleukin-6; Myogenic Regulatory Factors; Myosin...
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Cited By :13, Export Date: 21 July 2015, CODEN: MSMOF, Correspondence Address: Oliveira, S. A.; Departamento de Clínica Médica, Faculdade de Medicina de Botucatu, UNESP, Distrito de Rubião Júnior, s/n, 18618-000; Botucatu, SP, Brazil; email: oliveirajr.ufms@gmail.com, Chemicals/CAS: glucose, 50-99-7, 84778-64-3; insulin, 9004-10-8; interstitial col...

Citations

... Resistance to Obesity (OR) is a growing research topic in the scientific community with a considerable increase in the number of publications in recent years [1][2][3]. However, the etiology In this sense, the characterization of morphological and metabolic parameters, as well as the levels of physical activity and the lipid utilization pathways, as energy substrates for ATP generation, apparently predominant in OR animals, may be the key to understanding possible interventions and/or scientific and technological development that enable the creation and improvement of strategies to combat obesity and increase human performance. ...
... Lin et al indicated that PI3K activation protects the heart against myocardial infarction-induced heart failure, an event that takes place through positive effect of PI3K/Akt pathway activation on the gene expression of growth factor receptor-bound protein 14 in the heart (11). The ERK signaling pathway, belonging to the MAPK family, also represents a cascade containing a sequence of successive kinases that ultimately leads to phosphorylation and activation of terminal kinases, such as p38, JNK, and ERK (12,13). Furthermore, the ERK signaling pathway is initiated by several receptor families containing tyrosine kinase receptors (insulin growth factor receptors and fibroblast receptors), G protein receptors (angiotensin II, adrenergic receptors and endothelin-1), and cardiotrophin-1 (130 gp receptors) in cardiac myocytes; and the ERK signaling pathway is also caused by various stresses and tensile stimuli (14,15). ...
... Due to their susceptibility to oxidation, erythrocytes have been used as a cell model to investigate damage to biological membranes. Lipid peroxidation promoted by REs leads to the generation of MDA, a marker associated with a variety of diseases [28]. EBSp did not promote erythrocyte hemolysis, suggesting any toxic effects. ...
... AoS was surgically induced as previously described. [14][15][16][17] Rats were anaesthetized using a mixture of ketamine (50 mg/kg, IM) and xylazine (1 mg/kg, IM), and the heart was exposed via a median thoracotomy. A silver clip (0.62 mm internal diameter) was then placed on the ascending aorta approximately 3 mm from its root, constituting the AoS group (n= 17). ...
... AoS was surgically induced as previously described. [14][15][16][17] Rats were anaesthetized using a mixture of ketamine (50 mg/kg, IM) and xylazine (1 mg/kg, IM), and the heart was exposed via a median thoracotomy. A silver clip (0.62 mm internal diameter) was then placed on the ascending aorta approximately 3 mm from its root, constituting the AoS group (n= 17). ...
... 8 Classically, ectopic fat deposition around visceral vessels and organs has contributed to low-grade chronic inflammatory conditions and insulin resistance onset, which is also common in obesity. 9,10 In this aspect, perivascular adipose tissue has an essential paracrine function sustained by substantial protein activation and vascular morphological changes; these mechanisms contribute to the endothelial dysfunction during MS. 8,11 Consequently, clarifying the potential efficiency of therapeutic interventions is essential to manage MS conditions. ...