Diego A. Fabila-Bustos’s research while affiliated with National Polytechnic Institute and other places

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Publications (19)


Research Advances in Nanosciences, Micro and Nanotechnologies Vol. V. Prototype hydroponic system with LED lighting control and parameter measurement for various horticulture crops.
  • Chapter
  • Full-text available

March 2025

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65 Reads

Brian Emmanuel Ascención-Martínez

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Diego Adrián Fabila-Bustos

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Luis Felipe de Jesús Hernández Quintanar

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Among the main challenges that future generations will face is the scarcity of food and the lack of space for its cultivation. Hydroponics is an agricultural technique that is based on the independence of plants from the soil, since it uses only water as a means of transporting the substances and nutrients that crops need for their proper development, increasing efficiency and reducing the time in which crops are ready for harvest. In addition, the implementation of alternative lighting systems that allow the growth of plants without depending on solar illumination, being the use of LEDs one of the most popular and with the best results. This work describes the design process of a prototype hydroponic system with LED lighting control and measurement of parameters (temperature, pH, conductivity and dissolved oxygen) for various horticulture crops.

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General schematic view of the system.
Light source arrangement. (A) Phantom tissue. (B) LED with heatsink. (C) Homogeneous irradiation area.
Physical structure of the system. (A) Booth structure. (B) Arrangement of the LED supports. (C) Disk-type support base.
Image capture and electronic modules fixed on disk-type support base.
Electrical system connections.

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A Low-Cost Optomechatronic Diffuse Optical Mammography System for 3D Image Reconstruction: Proof of Concept

February 2025

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31 Reads

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2 Citations

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Alfredo Hernández-Mendoza

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Diego A. Fabila-Bustos

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[...]

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Background: The development and initial testing of an optomechatronic system for the reconstruction of three-dimensional (3D) images to identify abnormalities in breast tissue and assist in the diagnosis of breast cancer is presented. Methods: This system combines 3D reconstruction technology with diffuse optical mammography (DOM) to offer a detecting tool that complements and assists medical diagnosis. DOM analyzes tissue properties with light, detecting density and composition variations. Integrating 3D reconstruction enables detailed visualization for precise tumor localization and sizing, offering more information than traditional methods. This technological combination enables more accurate, earlier diagnoses and helps plan effective treatments by understanding the patient’s anatomy and tumor location. Results: Using Chinese ink, it was possible to identify simulated abnormalities of 10, 15, and 20 mm in diameter in breast tissue phantoms from cosmetic surgery. Conclusions: Data can be processed using algorithms to generate three-dimensional images, providing a non-invasive and safe approach for detecting anomalies. Currently, the system is in a pilot testing phase using breast tissue phantoms, enabling the evaluation of its accuracy and functionality before application in clinical studies.


Figura 10. Efecto de la adsorción del VM sobre caolín y la irradiación con luz UV.
Adsorción y fotólisis de verde de malaquita sobre arcilla caolinítica

December 2024

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52 Reads

PÄDI Boletín Científico de Ciencias Básicas e Ingenierías del ICBI

En este trabajo se investigó la adsorción y fotólisis del colorante verde de malaquita (VM) sobre una arcilla caolinítica de la región de Agua Blanca, Hidalgo. El estudio exploró el impacto de diversas variables del proceso, como la concentración de la arcilla, la concentración de VM, la radiación solar, la velocidad de agitación y la combinación de adsorción con luz UV. Los resultados mostraron que cada variable afecta de manera significativa la eficiencia de la degradación del colorante. Se llevaron a cabo experimentos con 100 mg de la arcilla los cuales fueron expuestos a 100 mL de soluciones de VM a diferentes concentraciones en un reactor de vidrio. Cabe mencionar que con la irradiación solar se alcanza una eficiencia del 42% en la remoción del VM, además de que existe una relación directa entre la velocidad de agitación y la eficiencia de remoción del colorante. La combinación de adsorción con la arcilla caolinítica y luz UV se identificó como un método más eficiente para la eliminación del VM en solución.


Análisis de un control crucero adaptable en condiciones de poca visibilidad

November 2023

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150 Reads

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1 Citation

PÄDI Boletín Científico de Ciencias Básicas e Ingenierías del ICBI

Este trabajo se presenta el análisis de un sistema de control crucero adaptable para vehículos robots. El sistema de control crucero adaptable propuesto ayuda a evitar choques por alcance en escenarios de poca visibilidad. La colisión se previene con el ajuste de velocidad, que está en función de la distancia del vehículo que se encuentre enfrente del vehículo robot. Se implementa con el uso de la visión artificial, el control adaptable, los sistemas embebidos, y el diseño asistido por computadora. Se analiza el funcionamiento del sistema propuesto mediante pruebas en terrenos a distintas inclinaciones, así como en escenarios controlados con visión favorable, y con visión deficiente. La visión deficiente es escenario clave para este trabajo, ya que, en caso de poca visibilidad en el ambiente, el sistema de visión artificial dejara de detectar vehículos. Entonces los sensores LiDAR y radar pasarán a medir distancia y velocidad del vehículo circulando al frente, y con estos datos, reducir o mantener la velocidad del vehículo.


Augmented reality to the creation of hybrid maps applied in soil sciences: a study case in Ixmiquilpan Hidalgo, Mexico

November 2023

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251 Reads

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1 Citation

The combination or mixture of methodologies offers so-called hybrid results, whose benefits can be focused on all areas of knowledge. In the present study, the creation of an Interactive Hybrid Maps Augmented Reality (AR) Application focused on teaching earth sciences was combined with edaphological, geographical, and cartographic information as a complement to online teaching due to the pandemic caused by COVID-19. Information was collected by studies carried out in the municipalities of Ixmiquilpan, Tasquillo, and Cardonal located at Mezquital Valley in Hidalgo, Mexico. The application was developed under evolutionary development models in combination with 2D maps, and Geographic Information Systems through the software Unity 3D graphics engine and AR Foundation SDK. In addition, laboratory results, information from historical databases, as well as field information, and pictures showing soil profiles of the study region were incorporated. The Interactive Hybrid Maps AR Application was installed and evaluated by Bachelor´s students of Biology with a specialty in soil sciences from the National Autonomous University of Mexico through a survey quiz that included items such as a learning tool, graphic 3D maps quality in augmented reality, easy handling, interaction and recommendable. Our results obtained a value of 0.90 Cronbach Alpha Coefficient indicating excellent reliability of the instrument. Moreover, an analysis of the last 10 years shows an incipient development of applications based on Augmented Reality for teaching earth sciences for university education. However, it is shown a wide development of AR applications in several fields since mathematics to museums.



Design of a Low-Cost Diffuse Optical Mammography System for Biomedical Image Processing in Breast Cancer Diagnosis

April 2023

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176 Reads

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5 Citations

Worldwide, breast cancer is the most common type of cancer that mainly affects women. Several diagnosis techniques based on optical instrumentation and image analysis have been developed, and these are commonly used in conjunction with conventional diagnostic devices such as mammographs, ultrasound, and magnetic resonance imaging of the breast. The cost of using these instruments is increasing, and developing countries, whose deaths indices due to breast cancer are high, cannot access conventional diagnostic methods and have even less access to newer techniques. Other studies, based on the analysis of images acquired by traditional methods, require high resolutions and knowledge of the origin of the captures in order to avoid errors. For this reason, the design of a low-cost diffuse optical mammography system for biomedical image processing in breast cancer diagnosis is presented. The system combines the acquisition of breast tissue photographs, diffuse optical reflectance (as a biophotonics technique), and the processing of digital images for the study and diagnosis of breast cancer. The system was developed in the form of a medical examination table with a 638 nm red-light source, using light-emitted diode technology (LED) and a low-cost web camera for the acquisition of breast tissue images. The system is automatic, and its control, through a graphical user interface (GUI), saves costs and allows for the subsequent analysis of images using a digital image-processing algorithm. The results obtained allow for the possibility of planning in vivo measurements. In addition, the acquisition of images every 30° around the breast tissue could be used in future research in order to perform a three-dimensional (3D) reconstruction and an analysis of the captures through deep learning techniques. These could be combined with virtual, augmented, or mixed reality environments to predict the position of tumors, increase the likelihood of a correct medical diagnosis, and develop a training system for specialists. Furthermore, the system allows for the possibility to develop analysis of optical characterization for new phantom studies in breast cancer diagnosis through bioimaging techniques.


Chemical structures of cantharidin (A) and its demethylated analog Norcantharidin (B)
A Survival plot for mice exposed to different doses of norcantharidin; and probability graphical representation of the estimated probit regression equation with 95% confident intervals, and prediction band. B Male mice. C Female mice
Graphical representation of reactive oxygen species (ROS) content in all tissues exposed to different norcantharidin doses. A Superoxide (O2.⁻). B Hydrogen peroxide content.* p < 0.05; ** p < 0.01
Hematoxylin and eosin micrographs multispectral object- and spatial-level quantitative analysis. A Contrasted nuclei by max entropy threshold and particle analysis. B Vein and adjacent sinusoidal capillaries analysis by Otsu´threshold. Pixel area analysis of multispectral data showed as pixel means ± standard deviation. * p ≤ 0.05; ** p ≤ 0.01 significant different compared to the control group
Norcantharidin toxicity profile: an in vivo murine study

October 2022

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60 Reads

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13 Citations

Naunyn-Schmiedeberg's Archives of Pharmacology

Norcantharidin (NCTD) is the demethylated analog of cantharidin, with allegedly reduced toxicity. However, there is still limited information regarding its posology and potential risk in its use in cancer treatment. Healthy BDF1 mice were intraperitoneally administered with norcantharidin (0, 3, 6, 12, and 25 mg/kg) every 24 h for 6 days. Survivor mice were euthanized, and the brain, lungs, kidneys, spleen, and liver were procured for enzymatic and histopathological analysis in the liver and kidney. DL50 were 8.86 mg/kg for females and 11.77 mg/kg for males. The treatments with 3.0 mg/kg and 6.0 mg/kg significantly modified the phosphorylase, alanine transaminase, and γ-glutamyl transferase activities; however, an organ-specific response was detected. A significant dose-dependent decrease was observed in the kidney for ROS, while the liver had the opposite effect. Histopathological analysis revealed a significant elevation in hepatocytes’ nuclei average size and total area (3 mg/kg), as well as centrilobular vein and adjacent sinusoidal capillaries showed a significant difference. The portal triad presented a significant difference in veins and capillarity count in 6 mg/kg. Renal samples showed cortex convoluted tubules’ average size significantly augmented in both doses’ groups, and tubule count was found augmented in 6 mg/kg. These physiological effects of NCTD can be exploited as treatment strategies if able to operate in an established posology and proper testing.


Comparison of development and characteristics of several educational tools in augmented reality for visualization of 3D models difficult to understand. Chemistry application case

August 2022

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80 Reads

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4 Citations

This paper shows the development of different scenarios in augmented reality (AR) using several programming tools, these applications were focused on visualization of 3D Bohr atomic models of the chemical elements from periodic table, organic and inorganic chemical molecules. The development tools were a) Unity3D in conjunction with the EasyAR SDK, b) HTML 5, CSS and the Google ARCore software development kit, c) Spark AR Studio and d) Adobe Aero with Glitch and HTML 5. The different scenarios created and described in this work were designed and developed to improve the learning of topics in an area of chemistry that requires three-dimensional models. The developments allowed 3D visualization and the interaction with the models to achieve several views of them, since the representations of these models are traditionally projected in books in two dimensions, making their understanding and interpretation in a not easy form.


Development of Virtual Reality Automotive Lab for Training in Engineering Students

August 2021

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1,101 Reads

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55 Citations

A Virtual Reality application was developed to be used as an immersive virtual learning strategy for Oculus Rift S Virtual Reality glasses and through Leap Motion Controller™ infrared sensors, focused on students of the Automotive Systems Engineering academic program, as a practical teaching-learning tool in the context of Education 4.0 and the pandemic caused by COVID-19 that has kept schools closed since March 2020. The technological pillars of Industry 4.0 were used to profile students so that they can meet the demands of their professional performance at the industrial level. Virtual Reality (VR) plays a very important role for the production-engineering sector in areas such as design and autonomous cars, as well as in training and driving courses. The VR application provides the student with a more immersive and interactive experience, supported by 3D models of both the main parts that make up the four-stroke combustion engine and the mechanical workshop scenario; it allows the student to manipulate the main parts of the four-stroke combustion engine through the Oculus Rift S controls and the Leap Motion Controller™ infrared sensors, and relate them to the operation of the engine, through the animation of its operation and the additional information shown for each part that makes it up in the application.


Citations (13)


... For geographical knowledge of soils to impact agriculture and environmental applications, it is necessary to create mobile technologies, such as apps for smartphones so the agronomists, engineers, producers, and students can use soil information expeditiously, quickly, cheaply, and efficiently (Lee et al., 2018;Ayala-Niño et al., 2023). On the other hand, it is necessary to improve communication between agricultural engineers and Maya farmers, some of whom have knowledge, and others have ancient wisdom about soils. ...

Reference:

A mobile application for smartphones with soil information: improving connectivity in terms of soil security
Augmented reality to the creation of hybrid maps applied in soil sciences: a study case in Ixmiquilpan Hidalgo, Mexico

... This study presents an optomechatronic system that integrates optical techniques, namely diffuse optical mammography (DOM) and three-dimensional (3D) reconstruction, to enhance the visualization and detection of abnormalities in breast tissue. The system builds upon the methodology proposed in [48], advancing it by incorporating a low-cost design capable of generating 3D reconstructions to provide detailed spatial information about abnormalities. This innovation addresses a critical need in medical diagnostics, particularly for conditions such as breast cancer, where the precise localization of anomalies significantly impacts patient outcomes. ...

Design of a Low-Cost Diffuse Optical Mammography System for Biomedical Image Processing in Breast Cancer Diagnosis

... It is an experimental drug, and its use in humans has not been well established, as it has not been approved by any regulatory entity. In a preliminary toxicological study in BDF1 mice, we established a lethal dose 50 (LD 50 ) of 8.8 mg/Kg and a therapeutic safe dose of 3 mg/Kg [31]. Notably, the concentration of NCTD found in serum samples was significantly lower than the safe dose proposed, suggesting that our formulation could support longer therapeutic schemes without exceeding toxicity thresholds. ...

Norcantharidin toxicity profile: an in vivo murine study

Naunyn-Schmiedeberg's Archives of Pharmacology

... In the context of assembly tasks, Offline authoring is the process in which the designer designs AR contents based on the assembly process. Designers utilize static or dynamic AR models, texts, images, and videos to pre-generate a series of operation sequences, operation positions, orientation, relevant constraints, etc. [177]. These AR contents are projected onto the real-world environment, allowing operators to directly observe virtual guidance within their physical surroundings. ...

Comparison of development and characteristics of several educational tools in augmented reality for visualization of 3D models difficult to understand. Chemistry application case
  • Citing Conference Paper
  • August 2022

... Oleh karena itu, pendekatan pembelajaran inovatif berbasis proyek dan teknologi, seperti Flexibility-Project Based Learning dan simulasi interaktif, menjadi sangat penting. Metode ini telah terbukti meningkatkan pemahaman dan keterampilan analitis murid dalam sistem kelistrikan otomotif (Hernández-Chávez et al., 2021;Muslim et al., 2021). ...

Development of Virtual Reality Automotive Lab for Training in Engineering Students

... By optimizing non-equidistant spacing demonstrated in this work, the uniformity could be significantly improved. In the work of Rojas Villafane et al. the uniformity of a LED based illumination system for medical imaging applications was improved to variations less of 6% by varying the position and angle of the LEDs [45]. Optimizing mounting heights and luminaire wattage resulted in an optimal design of plant lighting systems in the work Feerentinos and Albright [46]. ...

Design of a uniform irradiance source based on light emitting diodes
  • Citing Article
  • March 2021

Lighting Research and Technology

... [6] Fluorescence spectroscopy is appropriate for detection of the samples which contain fluorescent compounds. [7,8] Near-infrared spectroscopy is not applicable to analyze the samples with high water content (>80%), as a result of the strongly domination of sample spectra by the water signal. [9] Furthermore, these methods cannot detect the region of far-infrared spectral, but some of physical and chemical information were contained in far-infrared spectral, which concerned the materials. ...

Fluorescence spectroscopy on paraffin-preserved human liver samples to classify several grades of fibrosis
  • Citing Article
  • July 2020

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy

... Another example is the evaluation of oximetry measurements on intestinal walls for chronic mesenteric ischemia [13], where T-Stat 303 probes from Spectros were employed. Most fiber optic oximeters use a bundle of glass optical fibers to collect enough light from the remote tissue location [14]. These bundles have limitations for certain applications because direct contact between the fibers and tissue can cause injuries due to the stiffness of the glass. ...

Fiber-optic pulseoximeter for local oxygen saturation determination using a Monte Carlo multi-layer model for calibration
  • Citing Article
  • November 2019

Computer Methods and Programs in Biomedicine

... However, the disadvantage of DRS is that it possesses a very weak z resolution, and the measurement result represents the average property of a certain skin volume depending on the separation of the light source and detector as well as the individual's skin optical properties. By using appropriate algorithms, DRS allows identifying the absorption and scattering characteristics of biological tissue, and thus various tissue contents such as hemoglobin and water concentrations can be deduced [27][28][29][30][31][32] . Traditionally, photon diffusion theory is used for deep tissue studies where reflectance measurement setup is configured with large source detector separations (SDS), such as in breast cancer research 33 , and brain research 34 . ...

“Diffuse reflectance spectroscopy accurately discriminates early and advanced grades of fatty liver in mice,”
  • Citing Article
  • November 2018

Journal of Biomedical Optics

... Cai et al. developed a cost-effective multispectral imaging endoscope with an aim of studying the absorption of haemoglobin in the muscle tissue [13]. Josué et al. developed a light source unit with four different wavelengths operated through an optomechanical approach and were able to observe changes in the hepatic tissue for quantifying lipid levels [14]. ...

Multispectral light source for endoscopic procedures
  • Citing Conference Paper
  • February 2018