Diana Olthuis's research while affiliated with Radboud University Medical Centre (Radboudumc) and other places

Publications (9)

Article
Normal human keratinocytes (NHK) are widely used in the field of basic research, and conventional monolayer cultures have been a modality for almost thirty years (1). One common trade in the standard protocols is the use of antibiotics in the culture medium to reduce infection rates. Protocols have been optimized over the last decades regarding typ...
Article
Cutaneous squamous (pre)malignancies can be classified according to the keratinocytic intraepidermal neoplasia (KIN) classification. Aneuploidy can be seen as the result of chromosomal aberrations leading to altered DNA content and has been strongly associated with malignancy. Hyperproliferation is also strongly associated with tumorigenesis. The a...
Article
Avarol, a marine sesquiterpenoid hydroquinone, and 14 avarol derivatives have shown interesting anti-inflammatory properties in previous studies. In this study, avarol and derivatives were evaluated in high-throughput keratinocyte culture models using cytokeratin 10 and SKALP/Elafin expression as markers for respectively normal and psoriatic differ...
Article
Full-text available
Epithelia are barrier-forming tissues that protect the organism against external noxious stimuli. Despite the similarity in function of epithelia, only few common protective mechanisms that are employed by these tissues have been systematically studied. Comparative analysis of genome-wide expression profiles generated by means of Serial Analysis of...
Data
A detailed description of the Tissue Preferential Algorithm has been provided as supplemental material in a Word-document entitled "Materials supplement.doc".
Article
Mucosal secretions contain a range of defense effector molecules including antimicrobial peptides and proteinase inhibitors. These molecules play a central role in host defense against infection, and in a variety of immune and inflammatory reactions. The aim of this study was to analyze the levels of neutrophil defensins, the cathelicidin hCAP-18/L...
Article
Full-text available
Homozygosity for Cst6 null alleles causes the phenotype of the ichq mouse, which is a model for human harlequin ichthyosis (OMIM 242500), a genetically heterogeneous group of keratinization disorders. Here we report evidence for the mechanism by which deficiency of the cysteine protease inhibitor cystatin M/E (the Cst6 gene product) leads to distur...

Citations

... Even long-term storage is possible, since after seven days in DMEM at room temperature, keratinocytes can be generated. We recommend adding 1% antibiotic antimycotic solution to the transportation medium to prevent contamination of the cell culture, and the subsequent culture of the primary keratinocytes should be created without any addition of antibiotics, as this decreases the viability of the keratinocytes [25]. ...
... They consist of three families and cystatin M/E is a type 2 cystatin that is involved in the regulation of epidermal barrier function and differentiation of epidermal keratinocytes (Zeeuwen et al., 2001). Activities of cystatin M/E are mediated by cathepsins D, L, and V and legumain, which are then involved in the regulation of activities of transglutaminases 1 and 3. Transglutaminases can cross-link proteins and are crucially involved in the formation of cornified cell envelopes in the epidermal stratum corneum (Zeeuwen et al., 2004(Zeeuwen et al., , 2010Brocklehurst and Philpott, 2013). None of the parents of the affected mother showed any signs of KFSD and the variant in CST6 was not found in their samples, indicating autosomal dominant inheritance with a causative de novo variant in the index patient. ...
... Innate immunity in the reproductive tract plays an important role in the defense against Gram-negative bacteria, which usually are responsible for uterine infections. Neutrophils and epithelial cells produce neutrophil activating proteins (NAPs) that act as important immune responses against bacterial pathogens [102,103]. An example of NAPs in the mucus are defensins that play an important role in antibacterial activity, cell proliferation, and production of cytokines, chemokines, and modulation of adaptive and innate immune cells, which are moderated by estradiol and progesterone [79,104]. ...
... Kim et al. reported that SPRR3 promoted the proliferation of breast cancer cells by enhancing p53 degradation via the AKT and MAPK pathways [58]. Vos et al. reported that a subset of genes, such as SPRR2A, KRT6A, S100A2, and others, which are related and form an effective barrier against external (chemical and physical) stimuli, were expressed in bronchial epithelial cells and keratinocytes [59]. Our microarray data revealed similar changes in these genes (Fig. 6f). ...
... The pathological process of psoriasis is complex and has not been fully elucidated. Genetic, immune and environmental factors are all associated with the pathogenesis of psoriasis (Amigo et al. 2006;Saelee et al. 2011). The number of T cells increases in psoriasis (Bos et al. 1983); IL-22, which is primarily produced by activated T helper 1 cells, has been shown to be highly expressed in diseased skin from patients suffering from psoriasis (Nickoloff et al. 2007;Wolk et al. 2004), and cell models of psoriasis established using IL-22-stimulated HaCaT cells (keratinocytes) are widely used in the in vitro study of psoriasis (Liu et al. 2021;Jiang et al. 2017;Qiao et al. 2018a). ...
... Generally, photosensitizers cannot penetrate through the limitation of 2-mm depth [6]. Some reports also demonstrated a negative correlation between the thickness of the stratum corneum and photosensitizer penetration, indicating hyperkeratosis to be an important factor in intra-and interpatient differences in ALA photosensitizer uptake [7][8][9]. Importantly, current clinical PDT guidelines recommend pretreatment of Dihui Liu and Shuang Zhao contributed equally to this work. ...