David P. Lotshaw's research while affiliated with Northern Illinois University and other places
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Publications (8)
The mammalian family of two-pore domain K+ (K2P) channel proteins are encoded by 15 KCNK genes and subdivided into six subfamilies on the basis of sequence similarities: TWIK, TREK, TASK, TALK, THIK, and TRESK. K2P channels are expressed in cells throughout the body and have been implicated in diverse cellular functions including maintenance of the...
Multiple genes of the TASK subfamily of two-pore domain K(+) channels are reported to be expressed in rat glomerulosa cells. To determine which TASK isoforms contribute to native leak channels controlling resting membrane potential, patch-clamp studies were performed to identify biophysical and pharmacological characteristics of macroscopic and uni...
The hypothesis that Ca2+ influx necessary for angiotensin II (AngII) and K+ stimulation of aldosterone secretion is primarily mediated by membrane depolarization and activation of T-type Ca2+ channels was examined in isolated rat adrenal glomerulosa cells. Perforated-patch clamp recordings of membrane potential (Vm) demonstrated that AngII and K+ i...
1. The effects of the divalent cations Ca2+, Mg2+ and Ni2+ on unitary Na+ currents through receptor-regulated non-selective cation channels were studied in inside-out and cell-attached patches from rat adrenal zona glomerulosa cells. 2. External Ca2+ caused a concentration-dependent and voltage-independent inhibition of inward Na+ current, exhibiti...
1. The effects of the divalent cations Ca¥, Mg¥ and Ni¥ on unitary Na¤ currents through
receptor-regulated non-selective cation channels were studied in inside-out and cell-attached
patches from rat adrenal zona glomerulosa cells.
2. External Ca¥ caused a concentration-dependent and voltage-independent inhibition of
inward Na¤ current, exhibiting a...
The hypothesis that angiotensin II (ANG II)-induced aldosterone secretion is mediated through inhibition of plasma membrane K+ channels was examined by measuring the effects of K+ channel blockers on K+ currents, membrane potential, and aldosterone secretion in rat adrenal glomerulosa cells. Effective K+ channel blockers were identified and studied...
Nystatin perforated-patch clamp and single-channel recording methods were used to characterize macroscopic and single-channel K+ currents and the effects of angiotensin II (AngII) in cultured rat adrenal glomerulosa cells. Two basic patterns of macroscopic current-voltage relationships were observed: type 1 exhibited a rapidly activating, noninacti...
A Ca(2+)-permeant, nonselective cation channel was observed in cell-attached and inside-out membrane patches from rat adrenal glomerulosa cells maintained in primary cell culture. In cell-attached patches under near physiological ionic conditions, single-channel currents exhibited a reversal potential near -10 mV, inward rectification, a nearly lin...
Citations
... Other ion channel classes may also contribute to Ang II-induced depolarization. Lotshaw and Li (443) reported that Ang II activates Ca 2+ -permeant, nonselective cation channels, which may contribute to the depolarizing response in rat glomerulosa cells. ...
... Key Words: adrenal gland, primary aldosteronism, voltage-activated T-type calcium channel, CaV3. 2 ...
... maintain a negative resting membrane potential (−80 mV) close to the Nernst potential for potassium, which suggests that the membrane potential is determined mainly by the membrane potassium permeability. The potassium channels involved include the two pore-domain potassium channels, TWIK-related-acid-sensitive potassium family (TASK-1, TASK-3) and TWIK-related potassium channel 1, and the G protein-coupled, inwardly rectifying potassium channel Kir3.4 (23)(24)(25)(26)(27)(28). Elevated extracellular potassium levels depolarize the plasma membrane and activate the voltage-dependent T-type and L-type calcium channels (29)(30)(31)(32)(33)(34), leading to calcium influx and triggering signaling mechanisms described below for Ang II, including the activation of phospholipase D (PLD) (35). ...
... To verify that GRF2 and AnxA6 are mutually regulated in TNBC, we speculated that inhibition of Ca 2+ influx may differentially affect the expression of these proteins in AnxA6-high mesenchymal-like and AnxA6-low basal-like TNBC cells. To test this, we treated TNBC cells that express relatively high or low AnxA6 [17,22] with Ni 2+ , a non-selective Ca 2+ channel blocker (CCB) [23] or high Ca 2+ (3 mM). Treatment of AnxA6-high BT-549 TNBC cells with Ni 2+ led to a decrease in both AnxA6 and GRF2 protein levels, while Ca 2+ treatment had little or no effect (Fig 1A and 1B). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/567535972372480-1512322528111_Q64/Katherine-Sheehan-2.jpg)
... The potassium channels involved include the two pore-domain potassium channels, TWIK-related-acid-sensitive potassium family (TASK-1, TASK-3) and TWIK-related potassium channel 1, and the G protein-coupled, inwardly rectifying potassium channel Kir3.4 (23)(24)(25)(26)(27)(28). Elevated extracellular potassium levels depolarize the plasma membrane and activate the voltage-dependent T-type and L-type calcium channels (29)(30)(31)(32)(33)(34), leading to calcium influx and triggering signaling mechanisms described below for Ang II, including the activation of phospholipase D (PLD) (35). ...
... Several channel activators and deactivators have been identified so far that may be useful in the mitigation of diseases caused by K 2 P channel related malfunctions (Lesage and Lazdunski 2000;Lotshaw 2006;Veale et al. 2014). The identification, characterization and testing of new chemicals with antagonistic potential is time-consuming and involves sophisticated and expensive equipment. ...
... Macroscopic currents obtained from CGNs were studied using the whole-cell patchclamp configuration with a PC-501A amplifier (Warner Instruments, Hamden, CT, USA), as described previously [27]. The pCLAMP10 with an acquisition card (DigiData 1440, Molecular Devices, San Jose, CA, USA) was used for voltage protocols and data acquisition, Glass microelectrodes (3)(4)(5) were made from borosilicate capillaries using P97 Flaming/Brown Micropipette Puller (Sutter Instruments, Novato, CA, USA). The intracellular solution was composed of: 140 mM KCl, 10 mM HEPES, 5 mM EGTA, 2 mM K 2 -ATP, 1 mM MgCl 2 , and 0.5 mM CaCl 2 and adjusted to pH 7.4 with KOH. ...
