Darya S. Dubnyak's research while affiliated with National Research Center for Hematology and other places

Publications (21)

Article
Full-text available
Introduction. Chronic graft versus host disease (GVHD) is a frequently occurring complication after transplantation of allogeneic hematopoietic cells associated with a decrease in the quality of life and long-term administration of immunosuppressive drugs. Extracorporeal photopheresis (ECP) is a second line of therapy after treatment failure with g...
Article
Objective: Acute graft-versus-host-disease (aGVHD) develops in 10-80% of allo-HSCT patients. More than half of all aGVHD cases are refractory to first-line therapy with steroids. We hypothesized that bowel wall thickness at the time of aGVHD diagnosis could be an early sign of steroid-refractory aGVHD with gut involvement. Method: Our prospectiv...
Article
The graft-versus-host disease (GVHD) is among the most common complications after hematopoietic stem cell transplantation (allo-HSCT). The main tools for GVHD prevention remain calcineurin inhibitors (cyclosporin A, tacrolimus), methotrexate, mycophenolate mofetil. Upon implementation of reduced-intensity conditioning regimens, antithymocyte globul...
Article
Introduction T-regulatory cells (Treg) prevent the development of graft-versus-host disease (GVHD) after allo-HSCT (Beres & Drobyski, 2013). At the same time, this cell population can limit the anti-tumor response (graft-versus-leukemia, GVL), which can cause relapses. We report data of chimerism in a Treg population and the frequency of relapses i...
Article
Full-text available
The case report of botulinum toxin treatment of gastroparesis in a patient following allogenic bone marrow transplantation is described. The causes of gastroparesis and methods of prevention and treatment are discussed. It was noted that pyloric injection of botulinum toxin can improve symptoms and gastric emptying.
Article
Incidence, severity, and risk factors for hemorrhagic cystitis (HC) were assessed in 267 patients undergoing allogeneic hematopoietic stem cell transplantation (allo-HSCT). HC was diagnosed in 14.6% (39 patients) within 1-139 days after allo-HSCT (median duration – 39 days). Chemotherapy-related HC was diagnosed in 4 patients only. The majority (19...
Article
The aim of the study was to assess the efficacy of the phototherapy (UVA1 therapy and PUVA therapy) for the resistance to glucocorticosteroid and immunosuppressive treatment of cutaneous form of chronic graft-versus-host disease (chGVHD). The analysis of results of the phototherapy was performed. Twelve patients were included ih the study (2 patien...
Article
Introduction. Granzyme B is a serine protease commonly found in the granules of cytotoxic lymphocytes and natural killer cells. It is secreted with the pore forming protein perforin and mediates apoptosis in target cells. Granzyme B mediated cytolysis is one of the regulatory mechanisms (together with IL-2 receptors (CD25)) by which T-regulatory ce...
Poster
Abstract from X Raisa Gorbacheva Memorial Meeting “Hematopoietic Stem Cell Transplantation in Children and Adults”. For extended information please see original article at ”Leukemia Research” journal.
Poster
Abstract from X Raisa Gorbacheva Memorial Meeting “Hematopoietic Stem Cell Transplantation in Children and Adults”.
Poster
X Raisa Gorbacheva Memorial Meeting “Hematopoietic Stem Cell Transplantation in Children and Adults”.
Article
For many decades the main myeloablative conditioning regimen prior to allogeneic hematopoietic stem cell transplant (allo-HCT) consists of busulfan (16 mg/kg) and cyclophosphamide (120 mg/kg). The rate of transplanted-related mortality as a result of toxicity is still high. However alternative regimens are now avail-able. These regimens allow to re...
Article
Full-text available
The role of regulatory T-cells (T-reg) in the development of autoimmune complications after allogeneic bone marrow transplantation (allo-BMT) is to control the augmented response of donor effector cells. The main mechanisms of immune regulation are the secretion of cytokines, induction of metabolic disturbances in target cells, modification of dend...

Citations

... Extracorporeal photochemotherapy has been evaluated in small cohorts of patients with both acute and chronic GVHD. Responses were observed in skin, liver, gastrointestinal (GI) tract, mouth, eye, and lung11121314151617. Unfortunately, the literature is difficult to interpret because of the heterogeneity of treatment schedules and diagnostic and response assessment criteria . ...
... The association between granzyme B and immunosuppressive effects in Tregs was initially established based on the frequent presence of GrB + Tregs in malignant tumor lesions (17). Also, high levels of GrB + Treg was found to negatively correlated with the occurrence of acute graft-versus-host disease after hematopoietic stem cell transplantation, implying a regulatory effect on active participants of adaptive immunity (18). The expression and secretion of granzyme B by Tregs seem to be context-dependent, as evidenced by the disproportionate level of granzyme B in naturally occurring Tregs (nTregs) from thymus comparing to stimuli-inducible Tregs (iTregs) in TME (19). ...