Daniel Silberman's scientific contributions

Publications (8)

Data
After transduction with the DObWT chain, T cells from mice expressing DOb48A react with cells bearing the MHC allele that selected them. CD4 T cells were isolated from individual DOb48A mice and transduced with retorviruses expressing DObWT or DOb48A as described in the Methods section. The cells were vultured with spoleen cells from mice expressin...
Data
Data from individual mice show that both CD4 and CD8 T cells appear in mice expressing a single TCRb chain regardless of the MHC allele expressed. The numbers of thymus and lymph node cells were counted and analyzed for their expression of CD4 and CD8 and T cell receptor.
Data
In normal mice, a significant number of TCRα sequences appear on naïve CD4 T cells regardless of the selecting MHCII allele. Naïve CD4 T cells were isolated from the lymph nodes of normal mice of the indicated strains and their TCRα sequences identified as described in the Materials and methods section. Shown are the %s of unique sequences and the...
Article
Full-text available
Mature T cells bearing αβ T cell receptors react with foreign antigens bound to alleles of major histocompatibility complex proteins (MHC) that they were exposed to during their development in the thymus, a phenomenon known as positive selection. The structural basis for positive selection has long been debated. Here, using mice expressing one of t...
Preprint
Full-text available
Mature T cells bearing αβ T cell receptors react with foreign antigens bound to alleles of major histocompatibility complex proteins (MHC) that they were exposed to during their development in the thymus, a phenomenon known as positive selection. The structural basis for positive selection has long been debated. Here, using mice expressing one of t...
Article
Full-text available
Significance The evolutionary hypothesis for T-cell antigen receptor–peptide major histocompatibility complex (TCR–pMHC) interaction posits the existence of germ-line–encoded rules by which the TCR is biased toward recognition of the MHC. Understanding these rules is important for our knowledge of how to manipulate this important interaction at the...

Citations

... Majority of scTCR mutations are centred around the somatic driven CDR3, as germline fixed CDR1 and CDR2 loops are assumed to be invariant in a given individual. Even so, polymorphisms between individuals have been reported as evident from sequence data analysis (Marrack et al., 2017). The common belief is that germ line-encoded CDR1 and CDR2 loops are directed to increase the affinity of a TCR as they mainly interact with the MHC. ...
... For example, the associations between the genetic variability of MHC-I and the expression profiles of V-genes of TCRs of CD8 + lymphocytes are more significant in comparison with similar associations for CD4 + cells [124]. This is explained not only by the processes of coevolution [40], but also by closer contact between the V regions of the β-chain of TCRs of CD8 + cells and complementary regions of MHC-I molecules, which is determined by the difference in the spatial organization of TCRs of CD4 + and CD8 + lymphocytes [125][126][127]. This difference is established during maturation in the thymus, when the choice between CD4 and CD8 is determined by the TCR affinity for the corresponding class of MHC molecules [128]. ...