Cristina Pignata's research while affiliated with Università degli Studi di Torino and other places

Publications (54)

Article
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This study investigates the antibiotic resistance fate in the urban water cycle, evaluating the dynamics of antibiotic-resistant bacteria (ARB) and antibiotic-resistant genes (ARGs) in three different full-scale wastewater treatment plants (WWTPs) and two drinking water treatment plants (DWTPs) located in the same geographical area (North-West of I...
Article
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Background Wastewater reuse represents a promising alternative source of water supply considering the water scarcity related to climate change. However, if not adequately treated, wastewater represents a source of microbiological health risk. The purpose of this work was to investigate the role of wastewater treatment on microbiological contaminati...
Article
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Since many waterborne diseases are caused by human pathogenic viruses, virus monitoring of drinking water (DW) and DW sources is crucial for public health. Therefore, the aim of this review was to describe the occurrence of human pathogenic viruses in DW and DW sources; the occurrence of two viruses proposed as novel indicators of human faecal cont...
Article
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One source of water contamination is the release of wastewater that has not undergone efficient treatment. The aim of this study was to evaluate the reduction obtained with sodium hypochlorite (NaClO), UV and peracetic acid disinfection treatment of Salmonella spp., pathogenic Campylobacter, STEC and bacterial indicators in three full-scale municip...
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Over the past decade, several outbreaks of cryptosporidiosis have been reported in many EU countries in association with the consumption of contaminated drinking water, recreational waters, food consumption and contact with animals. The aim of this study was to evaluate the occurrence of Cryptosporidium oocysts in a drinking water treatment plant (...
Article
Background Biosensor technology can potentially revolutionise the area of air pollution monitoring by providing data on early biological effect induction. Such data can be developed for supplementing routine pollution monitoring, improving exposure estimation, including a potential effect estimation and raising community awareness about air polluti...
Article
The global action plan on antimicrobial resistance reports the necessity to develop standards and guidance for the presence of antimicrobial agents in the environment, especially in wastewater, highlighting its possible role in the antibiotic resistance spreading. In addition, the New European One Health Action Plan against Antimicrobial Resistance...
Article
Particulate matter (PM) is considered an atmospheric pollutant that mostly affects human health. The finest fractions of PM (PM2.5 or less) play a major role in causing chronic diseases. The aim of this study was to investigate the genotoxic effects of PM0.5 collected in five Italian towns using different bioassays. The role of chemical composition...
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Air pollution in urban areas is a major concern as it negatively affects the health of a large number of people. The purpose of this study was to assess the inhalation health risk for exposure to PM10 and benzene of the populations living in three Italian cities. Data regarding PM10 and benzene daily measured by “traffic” stations and “background”...
Article
Disinfection of hot water systems is critical for reducing Legionnaires’ disease in high-risk buildings. The use of neutral electrolysed oxidising water (NEOW) is a promising method for the control of microorganisms in hot water systems. However, full-scale evaluations of the efficacy of NEOW devices to control Legionella pneumophila are currently...
Article
Background: Recent data support the hypothesis that genetic damage occurring early in life during childhood can play an important role in the development of chronic diseases in adulthood, including cancer. Objectives: The objective of this paper, part of the MAPEC_LIFE project, is to describe the frequency of micronuclei and meta-nuclear alterat...
Article
Anaerobic digestion is a consolidated biotechnology able to produce renewable energy from biomasses. In the European countries, quick growth of biogas production from different organic matrices including wastes has been observed. In relation to the characteristics and quantity of the anaerobic digestion of feedstock, there are different technologie...
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Several studies have noted that treated and untreated wastewaters are primary contributors of a variety of pathogenic microorganisms to the aquatic ecosystem. Conventional wastewater treatment may not be sufficient to achieve microbiologically safe effluent to be discharged into natural waters or reused, thus requiring wastewater effluents to be di...
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Legionella pneumophila is a ubiquitous microorganism widely distributed in aquatic environments and can cause Legionellosis in humans. A promising approach to detect viable cells in water samples involves the use of quantitative polymerase chain reaction (qPCR) in combination with photoactivatable DNA intercalator propidium monoazide (PMA). However...
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Background Lifestyles profoundly determine the quality of an individual’s health and life since his childhood. Many diseases in adulthood are avoidable if health-risk behaviors are identified and improved at an early stage of life. The aim of the present research was to characterize a cohort of children aged 6–8 years selected in order to perform a...
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Food safety is a critical public health issue for consumers and the food industry because microbiological contamination of food causes considerable social and economic burdens on health care. Most foodborne illness comes from animal production, but as of the mid-1990s in the United States and more recently in the European Union, the contribution of...
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Preparation of the value-added products from e-waste resources is an important step in the recycling process. The present paper aims to propose a methodology for the recovery of In from scrap LCD panel via preparation of InBO3 nanostructure. Discarded LCD panel was subjected to a recycling process through crushing, milling, and oxalic acid leaching...
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INTRODUCTION: The MAPEC-Life project aims to study the biological effects of early exposure to air pollutants on the oral mucosa cells of school-age children in five Italian cities. A questionnaire was created to evaluate the association between outdoor and indoor airborne pollutants, lifestyle, diet and biomarker effects. The feasibility and relia...
Article
This study describes a multidisciplinary approach that investigates the breakdown potential of a laccase mediated system from Trametes pubescens MUT 2400 against several micropollutants including already recognized endocrine disrupting chemicals at their natural residual concentrations (from μg/L up to ng/L).
Article
Commonly the atmospheric pollution research is focussed on particulate indicators especially when mutagenicity was studied. On the other hand the volatile and semi-volatile compounds no adsorbed on to the particles can be genotoxic and mutagenic. Moreover some mutagenic compounds, such as polycyclic aromatic hydrocarbons, are present both in the pa...
Article
The aim of this study was to investigate the effect of plasma-enhanced chemical vapour deposition (PECVD) treatment on selected bacteria and spores and to contribute to the understanding of the synergistic effect of UV-directed plasma. The experiments were conducted on pure cultures of Aspergillus brasiliensis and Escherichia coli and on naturally...
Article
This paper focuses on the application of various biomonitoring techniques in China. We report a study in the Pearl River Basin (Guangzhou) based on the application of diatom indices as well as a study on the waterways in Wuhan based on evaluation of toxicity (using phytotoxicity, Daphnia magna and Microtox™ tests) and the Extended Biotic Index (EBI...
Data
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Objective Aim of this study was to investigate the relationships between the concentration of formaldehyde in air and the alkylation of hemoglobin to form a terminal N-methylenvaline residue in three occupationally exposed groups: a) technicians of pathology wards, b) workers of the plastic laminates industry, and c) a control group. All subjects r...
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The public has recently raised concerns regarding potential human health and environmental risks associated with tire crumb constituents in the artificial turf of football fields. The aim of the present study was to develop an environmental analysis drawing a comparison between artificial turf football fields and urban areas relative to concentrati...
Article
Aim of this study was to investigate the relationships between the concentration of formaldehyde in air and the alkylation of hemoglobin to form a terminal N-methylenvaline residue in three occupationally exposed groups: a) technicians of pathology wards, b) workers of the plastic laminates industry, and c) a control group. All subjects recruited i...
Article
Fine particles can be active carriers of toxic compounds into the alveoli of the lungs. Among these compounds are numerous mutagens and carcinogens. The direct mutagenicity per unit mass of fine particulate matter (PM) is significantly higher than that of coarse particles, especially in urban areas. In this study, the mutagenic properties of urban...
Article
This study investigates the impact of wastewater treatment plant (WWTP) effluent on the toxicity of the recipient water body and the effectiveness of the disinfection treatment applied (sodium hypochloride) to assure the compliance of both microbiological and toxicological emission limits. No toxicity was found in the majority of samples collected...
Article
Formaldehyde is an ubiquitous pollutant to which humans are exposed. Pathologists can experience high formaldehyde exposure levels. Formaldehyde-among other properties-induce oxidative stress and free radicals, which react with DNA and lipids, leading to oxidative damage and lipid peroxidation, respectively. We measured the levels of air-formaldehy...
Article
Semipermeable membrane device (SPMD) is a passive sampler that sequesters lipophilic contaminants, mimicking the bioconcentration in the fatty tissue of organisms. This study was designed to assess the use of SPMD and biological tests (Comet assay and Ames test) for air monitoring. For this purpose an occupational environment with expected polycycl...
Article
PM2.5 is the breathable fraction of the particulate matter and some adverse health effects, such as respiratory functionality, cardiological diseases and cancer, can be in some measure attributable to this risk factor exposure. Some of the most carcinogen compounds transported by PM2.5 are nitro-compounds. In this study, a strengthened in vitro bio...
Article
The present study investigated the biological quantification of estrogenic activity in the effluent of a wastewater treatment plant (WWTP) and in the recipient river in north-western Italy. Samples of the WWTP effluent and those of river water upstream and downstream the WWTP were taken from September 2006 to May 2007. The effluent was evaluated in...
Article
Endocrine disrupting chemicals (EDCs) have become a major issue in the field of environmental science due to their ability to interfere with the endocrine system. Recent studies show that surface water is contaminated with EDCs, many released from wastewater treatment plants (WWTP). This pilot study used biological (E-screen assay) and chemical (st...
Article
PM2.5 is one of the most important aspects of environmental health. This air pollutant is breathable and it is implicated in several chronic adverse health effects such as the decrease of respiratory functionality and cancer. Several in vitro bioassays are able to predict the mutagenic/carcinogenic activity of the environmental pollutants and mixtu...
Article
A preliminary study was performed to evaluate the role of formaldehyde (F) deriving from aspartame intake in the production of the adduct F-human serum albumin (F-HSA) by mean of a sera-epidemiological investigation. A blood-donors population (68 subjects) was analysed for the presence of anti-F-HSA IgG by an indirect competitive immunoenzymatic as...
Article
Epidemiological studies of particulate matter (PM) have associated PM mass, as well as certain individual components of PM such as secondary particulate with adverse human health effects. For example genotoxic effects attributed to PM may relate to the content of organic compounds but also to the oxidative DNA damage generated by transition metals...
Article
Owing to the large number of natural and anthropogenic sources, particulate matter (PM) may present several physical and chemical patterns in different areas. The finer PM2.5 fraction, which is now widely but not routinely measured in Europe, is considered to be the alveolar fraction of the ambient particles. Annual and winter mean concentrations o...
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Epidemiological studies have provided evidences for an association between exposure to elevated levels of ambient particulate matter (PM) and increased mortality and morbidity. However, the exact physiochemical nature of the responsible component is not clear. Secondary airborne PM formed from gas-phase pollutants contributes significantly to the m...
Article
A simplified method to detect faecal sterols, as an alternative assessment of environmental faecal pollution is proposed. The aim of this study is the development of a method to determine sterols in water samples avoiding sample filtration through glass fibre filter. The method is based on a liquid-liquid extraction and a final GC-FID determination...
Article
Aim of this pilot study was to correlate the human exposure to formaldehyde (F) with N-methylenvaline, a molecular adduct formed by addiction of F to the N-terminal valine in hemoglobin. A group of 21 subjects employed in a plywood factory and a laminate factory, and occupationally exposed to F, together with a group of 30 controls, were recruited...
Article
Semipermeable membrane devices (SPMDs) mimic passive diffusive transport of bioavailable hydrophobic organic compounds through biological membranes and their partitioning between lipids and environmental levels. Our study was developed on a surface water treatment plant based in Turin, Northern Italy. The investigated plant treats Po River surface...
Article
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Large segments of populations, including children, are exposed to environmental tobacco smoke (ETS), a risk factor for lung cancer and heart, circulatory and respiratory diseases. Recently, ETS was classified as a class A carcinogen by USEPA, as carcinogenic to humans by IARC (group 1) and by the National Toxicology Program of the US National Insti...
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Many xenobiotics, widely diffused in the environment, have the potential to disrupt the delicate endocrine system balance of wildlife and humans. Substances showing hormone-like actions in living organisms are defined endocrine-disruptor chemicals and they may mimic, block, or interfere with the synthesis, release, transport, elimination, and bindi...
Article
Human exposure to ethylene oxide (EtO) occurs mainly through inhalation of polluted air in occupational workplaces and/or via tobacco smoke. A significant biochemical reaction of EtO converts the terminal valine of hemoglobin into N-(2-hydroxyethyl)valine (HOEtVal). In the present study, the extent of HOEtVal formation in 360 healthy adults who wer...
Article
In research on Giardia cysts in surface water for human consumption samples which have been previously analysed using the EPA - IGR Laboratory Manual method based on indirect immunofluorescence, have been examined under the electronic microscope. Morphologically complete cysts have been observed in raw water (AG), but not in treated water (AP). Oth...
Article
Human exposure to ethylene oxide (EtO) occurs mainly through inhalation of occupational polluted air and tobacco smoke. EtO is able to react with DNA and proteins producing some molecular adducts. One of these, resulting from reaction between EtO and valine in hemoglobin, is N-(2-hydroxyethyl) valine (HOEtVal). This adduct represents a biological e...
Article
Considering its well-know toxicity and the chronic human exposure to lead, international law-makers enforced some directives or laws calling for the reduction of lead content of gasoline. All of these legislative acts aimed to reduce health risks for the general population. The aim of this study was to consider the effectiveness of these laws on ai...

Citations

... In the laboratory, one of the most common methods is the determination of the contents of constituents in the collected sample and their comparison with normative standards, which serve as a diagnostic method for the report (Bonetta et al. 2022). There are different methods of analysis described in the literature, such as physical processes linked to the separation and quantification of constituents, such as spectrometry, spectroscopy, turbidimetry and others, chemical processes that use chemical transformations as the primary basis for separation and quantification, highlighting volumetry, titration, combustion , gravimetry, and others, and also processes through gas and liquid chromatography, which are separation techniques that make use of physical and chemical methods for quantification and detection (Fan et al. 2023). ...
... Results obtained in the present study evidenced a possible genotoxic effect of fine PM2.5 on human lymphocytes, at all tested concentrations. Showed data seem to be concordant with results obtained by other authors although these last used different assays (chromosomal aberration test or Comet assay) and observed a significant increase of genotoxicity at generally higher concentrations of PM (for example 33, 100, 300 µg/mL) 14,41,42 . The mechanism by which PM induces genotoxicity, could be linked to the production of ROS. ...
... Although there was no excess cancer risk for male and female students and technicians, there is no safe level of carcinogen exposure below which there is no likelihood of having carcinogenic health effects throughout an individual's lifetime.94 Therefore, it is recommended to implement control measures such as the wearing of masks while in the workshop since the cancer risk limits are for regulatory purposes.46,95 ...
... Legionella detection (spp. and pneumophila) was carried out using a quantitative molecular PCR method as previously described [19]. Briefly, 20 mL of influent and 250 mL of different effluents were concentrated by filtration; DNA was extracted from filter using the DNeasy PowerWater Kit (Qiagen) and quantified using commercial kits (iQ-Check Quanti Legionella spp. ...
... years), although reported without available information on sampling season [81], are in accordance with this study. Also, in the study by Villarini et al. [82], MNi frequencies in school children were associated with the winter season and air pollution in the town where sampling was performed. ...
... Following the same reasoning, Traversi et al., shows that by green jobs one may understand all occupational jobs-from agriculture to administration and services-which contribute significantly to preserving or restoring the quality of the environment in terms of eliminating, reducing or mitigating the impact of pollution. The scale of such jobs is driven by the need to adopt methods of producing goods and services in an environmentally friendly manner to limit global warming and irreversible climate change [39]. ...
... However, its mode of action could dictate that PAA may potentially provoke oxidative stress to beneficial organisms in waterbodies receiving PAA-treated WW (Chhetri et al. 2014). Most studies evaluating the efficacy of PAA have so far only focused on the removal of microorganisms, indicating that its potential to remove EDC like E2 remains poorly known (Bonetta et al. 2017;Rizzo et al. 2019). There is no evidence, however, of any endocrine disruption potential of PAA itself in human health and ecotoxicological studies (Henao et al. 2018). ...
... It has been reported that dead cells can be differentiated from the intact cells before analyzing the samples via state-of-theart molecular technologies (Singh et al. 2018b;Vaishampayan et al. 2013). In these reports, propidium monoazide (PMA) was used as a viability marker to measure intact microorganisms before DNA was extracted and subjected to downstream molecular analyses (Bonetta et al. 2017; Kibbee and Örmeci 2017;Vesper et al. 2008;Weinmaier et al. 2015). The metagenomic sequences generated after treatment with PMA Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00253-019-09813-z) ...
... Three additional articles were included from twenty-six potentially relevant records identified by scanning the reference lists of included articles, and one was added from a narrative review of park prescription schemes [88]. Based on EPHPP guidelines, 16 of the 55 included papers were considered randomized controlled trials (RCTs) or controlled trials [12,[37][38][39]44,45,47,53,57,59,60,64,65,67,77,78], 3 were quasi-experimental studies [36,54,58], 32 were prospective observational studies [34,35,[40][41][42][43]46,[48][49][50][51][52]55,56,62,63,66,[68][69][70][71][72][73][74][75][80][81][82][83][84][85][86], 3 were retrospective longitudinal studies [61,76,87], and 1 was an uncontrolled pilot study [79]. Some studies combined data from control and experimental groups [35,40,82] and were considered observational because exposure to the intervention was not of substantive interest in the analyses. ...
... Applied to food, these electrical discharges destabilize cells, causing membrane damage, protein denaturation, lipid peroxidation, and DNA and/or RNA damage, among others [111]. In addition, it is a technique that does not leave any type of residue and does not raise the temperature of the treated product; thus, it does not greatly affect its original properties [112]. For this reason, cold plasma is being widely investigated for its use as an antimicrobial agent in food, antigerminative, toxin inactivator and biofilm control. ...