Clifford E Soll's research while affiliated with CUNY Graduate Center and other places

Publications (20)

Article
Full-text available
Phenolic glycolipids (PGLs) are polyketide synthase-derived glycolipids unique to pathogenic mycobacteria. PGLs are found in several clinically-relevant species, including various Mycobacterium tuberculosis strains, Mycobacterium leprae, and several nontuberculous mycobacterial pathogens, such as Mycobacterium marinum. Multiple lines of investigati...
Article
Juzen-taiho-to (JTT) is an immune-boosting formulation of ten medicinal herbs. It is used clinically in East Asia to boost the human immune functions. The active factors in JTT have not been clarified. But, existing evidence suggests that lipopolysaccharide (LPS)-like factors contribute to the activity. To examine this possibility, JTT was subjecte...
Article
Phenolic glycolipids (PGLs) are non-covalently bound components of the outer membrane of many clinically relevant mycobacterial pathogens, and play important roles in pathogen biology. We report a mutational analysis that conclusively demonstrates that the conserved acyltransferase-encoding gene papA5 is essential for PGL production. In addition, w...
Article
Full-text available
Melanoma is a malignancy with increasing incidence. Although primary tumors that are localized to the skin can be successfully treated by surgical removal, there is no satisfactory treatment for metastatic melanoma, a condition that has currently an estimated 5-year survival of just 6%. During the last decade, β- or α-emitter-radiolabeled peptides...
Article
Full-text available
The mycobactin siderophore system is present in many Mycobacterium species, including M. tuberculosis and other clinically relevant mycobacteria. This siderophore system is believed to be utilized by both pathogenic and nonpathogenic mycobacteria for iron acquisition in both in vivo and ex vivo iron-limiting environments, respectively. Several M. t...
Article
Full-text available
Phthiocerol dimycocerosates (PDIMs) and phenolic glycolipids (PGLs) are structurally related lipids noncovalently bound to the outer cell wall layer of Mycobacterium tuberculosis, Mycobacterium leprae, and several opportunistic mycobacterial human pathogens. PDIMs and PGLs are important effectors of virulence. Elucidation of the biosynthesis of the...
Article
A new diterpene with a fused 5-8-5 ring system was isolated from the fermentation broth of a soil actinomycete. The stereochemistry at C-15 was determined in an unusual manner using a decomposition product.
Article
Several Mycobacterium tuberculosis strains, Mycobacterium leprae, and other mycobacterial pathogens produce a group of small-molecule virulence factors called phenolic glycolipids (PGLs). PGLs play key roles in pathogenicity and host-pathogen interaction. Thus, elucidation of the PGL biosynthetic pathway will not only expand our understanding of na...
Article
The catalytic oxidation of alkenes by most iron porphyrins using a variety of oxygen sources, but generally not dioxygen, yields the epoxide with minor quantities of other products. The turnover numbers for these catalysts are modest, ranging from a few hundred to a few thousand depending on the porphyrin structure, axial ligands, and other reactio...
Article
Phenolic glycolipids (PGLs) are polyketide-derived virulence factors produced by Mycobacterium tuberculosis, M. leprae, and other mycobacterial pathogens. We have combined bioinformatic, genetic, biochemical, and chemical biology approaches to illuminate the mechanism of chain initiation required for assembly of the p-hydroxyphenyl-polyketide moiet...
Article
From Toki-shakuyaku-san, an herbal formulation for 'cleansing stagnated blood,' a key gene regulatory compound was purified and identified through a screening based on DNA microarray and real-time PCR.
Article
Ensuring biosecurity for pathogen-free rodents generally requires processing all materials that come in direct contact with the animals, including feed, to reduce or eliminate unwanted adventitious agents. A common method of processing animal feed is gamma irradiation. Irradiation is performed offsite and requires transport of feed from the irradia...
Article
We report an efficient and rapid means for the synthesis of tetrakis(pentafluorophenyl)porphyrin (TPPF(20)) derivatives by microwave irradiation in an environmentally acceptable solvent. The selective displacement of the para-fluorine groups in TPPF(20) by primary amines occurs in yields between 70 and 95%. This method demonstrates that TPPF(20) is...
Article
A screening methodology called 'genomic screening' was established to identify natural products that can regulate cellular gene expression. Application of genomic screening to Keishi-bukuryo-gan (KBG), a Japanese herbal medicine formulation, identified a previously unnoticed transcriptional effect by linoleic acid, a known KBG component. The approa...
Article
Full-text available
This commentary discusses the recent reports in RNA by Yan and colleagues and Westman and colleagues of the apparent failure of ribavirin to bind to recombinant eIF4E and inhibit 7-methyl guanosine cap-dependent exogenous mRNA translation of cell extracts in vitro. Measuring binding by using affinity chromatography of matrix-immobilized proteins an...
Article
Full-text available
Diacyl phthiocerol esters and their congeners are mycobacterial virulence factors. The biosynthesis of these complex lipids remains poorly understood. Insight into their biosynthesis will aid the development of rationally designed drugs that inhibit their production. In this study, we investigate a biosynthetic step required for diacyl (phenol)phth...

Citations

... However, the genes contained in Subnet 4, such as ppsa and ppsb, were significantly altered in drug-resistant bacteria (Cantrell et al., 2013). We believe that ppsa changes the expression of PDIM in the cell wall by changing the approach of the multi-subunit non-iterated polyketide synthase system (Vergnolle et al., 2015). This makes the bacterial cell wall thicker and causes bacterial drug efflux. ...
... However, while vegetable leaves are well populated with bacteria including potential probiotic acting Lactobacillus strains [34][35][36][37], so far, plant-derived produce has only been considered as vehicles of probiotic cultures [38,39]. A detailed understanding of how probiotic bacteria colonize leafy vegetables or herbs would enable future approaches to enrich these tissues with the respective bacterial strains to elevate vegetable leaves beyond their basic nutritional values. ...
... Phthiocerol dimycocerosate (PDIM) is one of the cell wall lipids of mycobacteria. The genes tesA, fadD26, ppsABCDE, mas, fadD28 could involve in the synthesis of PDIM (Chavadi et al., 2011(Chavadi et al., , 2012Vergnolle et al., 2015). Phthiocerol dimycocerosates (DIMs) and phenolic glycolipids (PGLs) are two structurally related families of the surface-exposed lipids, which have been proved contribute to the cell envelope permeability barrier and virulence (Astarie-Dequeker et al., 2009;Cox et al., 1999). ...
... The melanin pigment itself may also be a specific target for antibodies or peptides, as demonstrated in melanomas (Norain & Dadachova, 2016 (Dadachova et al., 2008;Klein et al., 2013;Revskaya et al., 2009;Thompson et al., 2014) (Ballard et al., 2011;Dadachova et al., 2006). [ 188 Re]6D2 mAb was also tested in one phase I clinical trial (Klein et al., 2013) and has shown good tolerance, localization in melanoma metastases and antitumor activity. ...
... Moreover, the mutant failed to grow in the macrophage cell line as well as in THP-1 cells. 107 Chavadi et al. 94 (2011) reported that ΔmbtA, ΔmbtB, ΔmbtC, ΔmbtD, ΔmbtE, ΔmbtF, ΔmbtG, and ΔmbtT mutant Msmeg strains were not able to produce an appreciable amount of mycobactin, indicating the essentiality of Mbt ABCDEFGT NRPS-PKS enzymatic machinery in the biosynthesis of the core scaffold of the mycobactin. Bythrow et al. 108 constructed Msmeg ΔEM (deficient of both exochelin and mycobactin gene) which failed to show growth in an iron-limiting environment (GAS media, pH 6.6). ...
... We therefore focused on the 11 prioritized targets identified at pH 6.6 as those most likely to be relevant to the antibacterial activity of AA692. Four have known or predicted functions in mycomembrane lipid biosynthesis: the mycolyltransferases FbpA and FbpB (Belisle et al., 1997); the thioesterase TesA (Alibaud et al., 2011;Chavadi et al., 2011); and the lipase Rv3802c (Parker et al., 2009). In addition, the predicted penicillin-binding protein Rv1730c likely maintains peptidoglycan and thus sustains cell wall integrity. ...
... They were first isolated as glycosides from the phytopathogenic fungus Fusicoccum amygdali, in 1964 [81]. Substances exhibiting this structural motif have been isolated from a variety of sources including fungi such as Talaromyces purpureogenus, Alternaria brassicicola XXC, and Trichoderma citrinoviride cf-27 ( Figure 3), and rarely from liverworts, algae, ferns, streptomycetes, and higher plants, some of which showed remarkable biological effects relevant for drug discovery, such as antibacterial, antitumor, anti-inflammatory, and antifungal activities [82][83][84][85][86][87][88][89]. A new diterpenoid roussoellol C 26 that exhibited moderate antiproliferative activities against human breast adenocarcinoma (MCF-7) cell line with an IC50 value of 6.5 μM has been isolated from an extract of laboratory cultures of the marine-derived fungus Talaromyces purpurogenus [90]. ...
... The rv2931/ppsA gene was the most-highly upregulated (9.3fold) in Erdman relative to ∆sigC ( Figure 4). It encodes a phenol-phthiocerol polyketide synthase that functions in phthiocerol dimycocerosates (PDIM) biosynthesis [32]. The elevated expression of ppsA in Erdman cultured in SMT medium but not when sigC was artificially induced in 7H9 medium suggests an indirect effect of sigC on ppsA expression, which will be discussed later. ...
... [1,2] As such, it is useful across the board, from bulk chemicals anda grochemicals [3,4] all the way to fine chemicals and fragrances. [5][6][7] In theory,a llylic oxidation is as traightforward exothermic reaction. It requires only O 2 ,afree, eco-friendly and widely available reagent. ...
... It has been demonstrated that metalloporphyrins easily bind to nucleic acids, leading to fracture in DNA strands (Pitie et al., 1992). Cationic porphyrins are known as functional compounds that attach to DNA and photodynamically modify the intended site of a DNA molecule by a mechanism similar to that of anti-tumor drugs (Drain et al., 1999). Phthalocyanines (Pcs), having a very similar structure with that of porphyrins, also have been widely studied. ...