Claudia Böhland's research while affiliated with OncoRay- Center for Radiation Research in Oncology and other places

Publications (22)

Article
Full-text available
Background Invasiveness is a major factor contributing to metastasis of tumour cells. Given the broad variety and plasticity of invasion mechanisms, assessing potential metastasis-promoting effects of irradiation for specific mechanisms is important for further understanding of potential adverse effects of radiotherapy. In fibroblast-led invasion m...
Article
Although monosodium urate (MSU) crystals are known to trigger inflammation, published data on soluble uric acid (sUA) in this context are discrepant. We hypothesized that diverse sUA preparation methods account for this discrepancy and that an animal model with clinically relevant levels of asymptomatic hyperuricemia and gouty arthritis can ultimat...
Article
Full-text available
Crystallopathies are a heterogeneous group of diseases caused by intrinsic or environmental microparticles or crystals, promoting tissue inflammation and scarring. Certain proteins interfere with crystal formation and growth, e.g., with intrarenal calcium oxalate (CaOx) crystal formation, a common cause of kidney stone disease or nephrocalcinosis-r...
Data
Anti-TGFβ IgG treatment decreases oxaluria but has no effect on calcinuria. C57BL/6N mice were either fed a control diet (control), a high-oxalate diet (oxalate only) or an oxalate-rich diet combined with preemptive IgG1 or anti-TGFβ antibody treatment (oxalate + IgG1 and oxalate + anti-TGFβ) for up to 14 days. Serum and urine oxalate levels (A) an...
Data
Delayed anti-TGFβ IgG treatment does not improve renal function in chronic oxalate nephropathy. (A) Flow diagram of experimental design: C57BL/6N mice were fed an oxalate-rich diet combined with delayed IgG1 or anti-TGFβ treatment (total of 4 i.p. injections) (oxalate + IgG1 or oxalate + anti-TGFβ) compared to control diet (control) for 14 days. (B...
Data
Depletion of Jarid1A does not lead to strong induction of p21. Expression of p21 after depletion of Jarid1A in HeLa, MCF-7 and U2OS cells, 72 h after transfection with scr or Jarid1A siRNA. A representative sample and its quantitative evaluation are shown. Cont = untransfected control. (TIF)
Article
Full-text available
Histone demethylases have recently gained interest as potential targets in cancer treatment and several histone demethylases have been implicated in the DNA damage response. We investigated the effects of siRNA-mediated depletion of histone demethylase Jarid1A (KDM5A, RBP2), which demethylates transcription activating tri- and dimethylated lysine 4...
Data
Depletion of Jarid1A does not affect anti-correlation of γH2AX and H3K4me3 or active RNA Pol II, respectively, after ion irradiation. HeLa cell transfected with scr or Jarid1A A1+A3 siRNAs were subject to ion microirradiation with single carbon ions applied in line patterns (lateral distance between single ion hits 1 μm, distance between “lines” 5...
Data
Comparison of relative band intensities corresponding to Jarid1A and Jarid1B proteins. Band intensities were normalized with respect to Tubulin loading control. Intensities in HeLa cells were set as 100%. All bands to be compared were on the same blot. Data are from 2 Western Blots. (DOCX)
Data
Background and residual 53BP1 and Rad51 foci are not affected by depletion of Jarid1A. Mean background frequency and number of residual 53BP1 and Rad51 foci (+/- SEM) were determined in at least 20 cells after 5 Gy X-irradiation. Cells were fixed before irradiation or at 24 h and 48 h after irradiation and indirect immunofluorescence was performed....
Data
MNase accessibility is not affected by Jarid1A depletion. Analysis of chromatin accessibility by MNase digestion of isolated nuclei. After different incubation periods with 0.5 u MNase, comparable amounts of partially digested DNA were loaded onto an agarose gel. The emerging ladder of mono- and oligonucleosomes is comparable in both samples indica...
Data
Efficient down-regulation of Jarid1A is associated with a global increase of H3K4me3. (A) Decreased levels of Jarid1A in whole cell protein extracts of HeLa, MCF-7 and U2OS cells after siRNA transfection. Western blot images show levels of Jarid1A in untransfected cells (cont), in cells transfected with scrambled siRNA (scr) and in cells transfecte...
Data
Depletion of Jarid1A enhances radiosensitivity. Colony formation experiment with Hela cells transfected with JaridA1 siRNAs A1 or A3 or a combination thereof, as well as untransfected controls and cells transfected with scr siRNA. Cells were irradiated 72 h after siRNA transfection with 0 Gy, 2 Gy, 5 Gy or 10 Gy X-rays. Cells were incubated for 10...
Data
Early formation of γH2AX foci is not affected by depletion of Jarid1A. 72 h after transfection with scr or Jarid1A A1+A3 siRNA, HeLa cells were irradiated in a small angle configuration with 55 MeV carbon ions and fixed after 5 min (A) and 2 min (B), or irradiated with 5 Gy X-rays and fixed after 15 min (C). Indirect immunofluorescence was performe...

Citations

... Similarly, Miyazaki et al. successfully built a model using a threedimensional collagen matrix, demonstrating the important role of CAFs in cancer invasion [9]. In addition, a kind of ultralow adhesion plate has been developed and used in research on the effects of radiation on invasiveness [10]. The formation of cellular aggregates is similar to their behavior in vivo [11], which demonstrates the advantage of threedimensional coculture in monitoring the crosstalk of CAFs/ NFs and cancer cells. ...
... This negative impact of hyperuricemia on neutrophil function was a direct result of increased soluble urate uptake into neutrophils through urate transporters, resulting in changes to intracellular pH and cytoskeletal dynamics [45]. Soluble urate has also been shown to negatively regulate monocyte activation in MSU crystal-induced tissue inflammation [46]. Collectively, these results suggest that soluble urate can both promote and suppress inflammation. ...
... Data were analyzed using MPD Lab software (MediBeacon, Mannheim, Germany). The GFR [μl/min] was calculated from the decrease of fluorescence intensity over time using a two-compartment model, the animals' body weight, and an empirical conversion factor [76]. ...
... Through binding to HDAC1, KDM5B binds to the repressive LSD1/NURD deacetylase complex and shows co-occupancy with the NuRD complex on chromatin [159,168]. KDM5A and KDM5C also bind to HDAC-containing complexes [169,170] and the activities of demethylation and acetylation are interlinked [171]. The combination of LSD1 and KDM5 proteins ensures the complete demethylation of H3K4 and, coupled with HDACs, this provides a very strong repressive signal. ...