Ciarra Almeria's research while affiliated with University of Natural Resources and Life Sciences Vienna and other places

Publications (5)

Article
Full-text available
Extracellular vesicles (EVs) are cell-derived membrane structures exerting major effects in physiological as well as pathological processes by functioning as vehicles for the delivery of biomolecules to their target cells. An increasing number of effects previously attributed to cell-based therapies have been recognized to be actually mediated by E...
Chapter
This chapter will provide an overview on scaffold-free 3D models focusing on aggregates, spheroids, and organoids. While often used synonymously, the differences are presented as well as the origin and advancement until the current date. Moreover, the advantages that scaffold-free systems offer and their limitations are discussed as well as fields...
Chapter
When starting to work with 3D cell cultures it is essential to be aware of the differences to common 2D cultures in terms of expenses and equipment as well as the type and dimension of 3D you intend to work with. The various culture methods require different equipment, efforts, and awareness on handling and analytics. Therefore, it is crucial to ch...
Article
Full-text available
Mesenchymal stem/stromal cells (MSCs) display a variety of therapeutically relevant effects, such as the induction of angiogenesis, particularly under hypoxic conditions. It is generally recognized that MSCs exert their effects by secretion of paracrine factors and by stimulation of host cells. Furthermore, there is increasing evidence that some th...

Citations

... While traditional bioreactor systems are ideal for cells culture in suspension, attached cell monolayers require different solutions. For these cell types, advanced bench top culture systems providing Table S1), which are also expandable in terms of replication, will likely provide the best capacity for adherent cell types (see, Kreß et al., 2021). We provide considerations as well as suggestions for improved environmental control for culture systems ranging in complexity from batch cultures to bioreactors (Table 1 and Supplementary Table S2). ...
... Therefore, either medium with a complex chemical composition must be produced to allow for scale expansion, or the initial number of cells must be increased to critical levels in another medium before the cells are transferred to this serumfree medium for EVs production [21]. Additionally, studies have been reported on the effect of environmental conditions on the media, with oxygen content (reduced from the normal 21 to 2-7%), shear stress, and stimulation with proinflammatory cytokines (IFN-γ and TNF-α) all reported to enhance the quality or quantity of secreted EVs [27,28]. Environmental conditions are difficult to standardize for the industrial-scale production of EVs because EVs production varies by cell. ...