Chantal Gondran's research while affiliated with French National Centre for Scientific Research and other places

Publications (65)

Article
For four decades, the functionalization of electrodes by biomaterials based on electrogenerated polymers, carbon nanotubes and / or nano-objects, was widely used in the field of analytical chemistry and energy conversion. Recently, we redesigned enzymatic bioelectrodes in order to produce original objects. An innovative concept of reagentless biose...
Article
A biosensor based on the release of the enzyme substrate from its structure was developed for the inhibitive detection of benzoic acid. A polyurethane support comprising two perforated microcapsules (800 μm in diameter) filled with methylene blue as a model compound and covered with a conductive deposit of multiwalled carbon nanotubes, continuously...
Article
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A homemade gold electrode is modified with a carbon nanotubes/gold nanoparticles nanocomposite to perform selective and sensitive electrochemical detection of dengue toxin. This nanostructured composite offers a large specific surface and a reactive interface allowing the immobilization of biological material. Dengue antibodies are immobilized on g...
Article
WS2 nanotubes functionalized with carboxylic acid functions (WS2-COOH) were used for improved immobilization of the enzyme tyrosinase in order to form an electrochemical biosensor towards catechol and dopamine. The nanotubes were deposited on glassy carbon electrodes using a dispersion-filtration-transfer procedure to assure the reproducibility of...
Article
The natural biodegradabilty of porous silicon (pSi) in physiological media limits its wider usage for implantable systems. We report the stabilization of porous silicon (pSi) membranes by chemical surface oxidation using RCA1 and RCA2 protocols, which was followed by a PEGylation process using a silane-PEG. These surface modifications stabilized th...
Article
A high performance impedimetric immunosensor for the dengue virus antibody detection is presented. The setup profits from the formation of controlled and reproducible carbon nanotube (CNT) deposits on electrodes. Their easy functionalization via electrogeneration of a polypyrrole-NHS (N-hydroxysuccinimido 11-(pyrrol-1-yl) undecanoate) film enables...
Article
An original impedimetric immunosensor was developed based on carbon nanotube (CNT) deposits with controlled thicknesses for enhanced electroactive surface areas leading to improved sensor performances. Cholera monitoring was chosen as the model immune system for this setup. These CNT deposits were characterized using confocal laser microscopy and e...
Article
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Engineering bioelectronic components and set-ups that mimic natural systems is extremely challenging. Here we report the design of a protein-only redox film inspired by the architecture of bacterial electroactive biofilms. The nanowire scaffold is formed using a chimeric protein that results from the attachment of a prion domain to a rubredoxin (Rd...
Article
A photoelectrode was designed by electrodeposition of a pyrrole monomer modified with a polypyridyl Ru(II) complex bearing benzo[i]dipyrido-[3,2-a:2′.3′-c]phenazine (dppn) ligand. Owing to the intercalating properties of these immobilized complexes towards DNA double helix, cocaine aptamer was immobilized on the modified electrodes thanks to its st...
Article
An electrochemical highly sensitive aptasensor was developed based on electropolymerized poly(pyrrole-nitrilotriacetic) acid film and a new aptamer functionalized by a pentahistidine peptide for the quantification of bisphenol A. A surface coverage of anti-bisphenol A aptamer of 1.84×10-10 mol cm-2 was estimated from the electrochemical signal of t...
Patent
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A biofuel cell intended to be immersed into a liquid medium containing a sugar and oxygen, wherein the anode includes an enzyme capable of catalyzing the oxidation of the sugar and a redox mediator of low redox potential capable of exchanging electrons with the anode enzyme and the cathode includes an enzyme capable of catalyzing the reduction of o...
Patent
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A novel cell including first and second chambers containing a solvent and separated by a wall permeable to the solvent and impermeable to hydronium and/or hydroxyl ions; a first electrode in the first chamber; a second electrode in the second chamber; a first redox couple in the first chamber comprising a first oxidizer and a first reducer taking p...
Article
The design of photoactive functionalized electrodes for the sensitive transduction of double-stranded DNA hybridization is reported. Multifunctional complex [Ru(bpy-pyrrole)2(dppn)]2+ (bpy-pyrrole=4-methyl-4′-butylpyrrole-2,2′-bipyridine, dppn=benzo[i]dipyrido[3,2-a:2′,3′-c]phenazine) exhibiting photosensitive, DNA-intercalating, and electropolymer...
Article
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This paper reports on the impedimetric transduction of binding reaction between polymerized saccharides and target lectins. The controlled potential electro-oxidation of pyrrole-lactosyl and pyrrole-3'-sialyllactosyl at 0.95 V vs. Ag/AgCl, provides thin and reproducible poly(pyrrole-saccharide) films. The affinity binding of two lectins: Arachis hy...
Article
Full-text available
We describe the first implanted glucose biofuel cell (GBFC) that is capable of generating sufficient power from a mammal's body fluids to act as the sole power source for electronic devices. This GBFC is based on carbon nanotube/enzyme electrodes, which utilize glucose oxidase for glucose oxidation and laccase for dioxygen reduction. The GBFC, impl...
Article
Full-text available
We describe the first implanted glucose biofuel cell (GBFC) that is capable of generating sufficient power from a mammal's body fluids to act as the sole power source for electronic devices. This GBFC is based on carbon nanotube/enzyme electrodes, which utilize glucose oxidase for glucose oxidation and laccase for dioxygen reduction. The GBFC, impl...
Article
Full-text available
We report the design of a novel glucose/O2 biofuel cell (GBFC) integrating carbon nanotube-based 3D bioelectrodes and using naphthoquinone-mediated oxidation of glucose by glucose oxidase and direct oxygen reduction by laccase. The GBFCs exhibit high open circuit voltages of 0.76 V, high current densities of 4.47 mA cm-2, and maximum power output o...
Article
We describe herein the fabrication of bioelectrodes by compression of a mixture of graphite particles, enzyme and redox mediator that leads to the immobilization and electrical connection of enzymes. Bioanodes were elaborated by combining graphite with glucose oxidase and ferrocene while biocathodes resulted from the association of graphite, laccas...
Article
A label-free and highly sensitive impedimetric aptasensor was developed based on electropolymerized film for the determination of thrombin. The first step is the electrogeneration of a poly(pyrrole-nitrilotriacetic acid) (poly(pyrrole-NTA)) film onto the surface of electrodes followed by complexation of Cu(2+) ions. Then, the histidine labeled thro...
Article
Full-text available
Reaction of dimeric [Rh(II)(2)(phen)(2)(μ-OAc)(2)(MeCN)(2)](BF(4))(2) (phen =1,10-phenanthroline) with pyrazine (pz) in a 1:2 ratio leads to the new 1-D metal-metal-bonded coordination oligomer {[Rh(II)(2)(phen)(2)(μ-OAc)(2)(pz)](BF(4))(2)}(n) (Rh-Rhpz)(n) (1), where each Rh atom of the dimeric unit (Rh-Rh) is coordinated in the equatorial plane to...
Article
Full-text available
Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and determination of the affinity constant.
Article
Tethered bilayer lipid membranes (tBLMs) are designed on mixed self-assembled monolayers (SAMs) of a novel synthetic anchoring thiol, 2,3-di-o-palmitoylglycerol-1-tetraethylene glycol mercaptopropanoic acid ester (TEG-DP), and a new short dilution thiol molecule, tetraethylene glycol mercaptopropanoic acid ester (TEG). tBLM formation was accomplish...
Data
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Supplementary Figures S1-S2 and Supplementary Notes 1-3.
Article
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Enzymatic fuel cells use enzymes to produce energy from bioavailable substrates. However, such biofuel cells are limited by the difficult electrical wiring of enzymes to the electrode. Here we show the efficient wiring of enzymes in a conductive pure carbon nanotube matrix for the fabrication of a glucose biofuel cell (GBFC). Glucose oxidase and la...
Article
A novel and simple immobilization strategy for biotinylated biological macromolecules onto electro-polymerized poly(pyrrole-nitrilotriacetic acid)(NTA)–Cu 2+ films without avidin as connecting bridge is reported. After complexation of Cu 2+ by the polymerized NTA chelator, biotinylated biomolecules were immobilized by coordination of the biotin gro...
Article
Impedance spectroscopy transduction combined with the immunosensor technology has been used for the determination of atrazine, a herbicide The sensor electrode was based on the immobilization of anti-atrazine antibody by affinity binding onto a polypyrrole film N-substituted by nitrilotriacetic acid NTA electrogenerated on a gold electrode. The pol...
Article
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In this thesis work, we were interested in the development of electrochemical biosensors by using iron rich clays and Layered Doubles Hydroxides (LDH), as matrices for immobilizing hemoglobin (Hb). The aim of this work was to study the contribution of iron structural materials in improving the performance of biosensors by a phenomenon of redox cata...
Article
Full-text available
Powering future generations of implanted medical devices will require cumbersome transcutaneous energy transfer or harvesting energy from the human body. No functional solution that harvests power from the body is currently available, despite attempts to use the Seebeck thermoelectric effect, vibrations or body movements. Glucose fuel cells appear...
Data
Supplementary Materials and Methods for A Glucose BioFuel Cell Implanted in Rats. (0.07 MB DOC)
Article
We describe herein the synthesis and electrochemical polymerization of a viologen monomer functionalized by a biotin group and the use of its redox properties for the impedimetric sensing of protein binding. The electrochemical oxidation of the pyrrole group at 0.85 V in CH3CN led to a biotinylated polypyrrole film allowing the successive anchoring...
Article
An ultrahigh performance impedimetric DNA sensor is presented showing detection limits in the femtomolar range. This electrochemical setup was constructed initially by electrogeneration of poly(11-pyrrol-1-yl-undecanoic acid N(alpha'),N(alpha)-bis(carboxymethyl)-L-lysine amide) (poly(pyrrole-NTA)) film. The latter was then modified by the coordinat...
Article
This paper describes the construction of an impedimetric immunosensor for the label-free detection of ciprofloxacin, an antibiotic belonging to synthetic fluoroquinolones. A poly(pyrrole-N-hydroxysuccinimide) film was electrogenerated onto electrodes and then used for the reagentless covalent binding of a fluoroquinolone model bearing an amino grou...
Article
The electrochemical properties of natural or synthetic ferruginous clays, nontronite, montmorillonite and saponite, were studied by cyclic voltammetry and impedancemetry. Clay modified electrodes were then tested for the reduction of hydrogen peroxide under different conditions: in acidic medium without redox mediator, in phosphate buffer solutions...
Article
The paper reports on the investigation of the electrochemical behavior of a thin gold film electrode coated with silicon dioxide (SiOx) layers of increasing thickness. Stable thin films of amorphous silica (SiOx) were deposited on glass slides coated with a 5nm adhesion layer of titanium and 50nm of gold, using plasma-enhanced chemical vapor deposi...
Article
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This paper reports on the use of electrochemical surface plasmon resonance (E-SPR) for the detection of carbohydrate-binding proteins. The generation of an SPR sensor specific to lectins Arachis hypogaea (PNA) and Maackia amurensis (MAA) is based on the electrochemical polymerization of oligosaccharide derivatives functionalized by pyrrole groups....
Article
This chapter presents a procedure for electrochemical synthesis of a biotinylated polypyrrole co-polymer film and electrochemical investigations for the determination of the most appropriate enzyme marker. Preparation of the electropolymerized biotinylated copolymer film include following steps: (1) electrolysis of a mixture of pyrrole-lactobioamid...
Article
Impedance spectroscopy approaches combined with the immunosensor technology have been used for the determination of trace amounts of ciprofloxacin antibiotic belonging to the fluoroquinolone family. The sensor electrode was based on the immobilization of anti-ciprofloxacin antibodies by chemical binding onto a poly(pyrrole-NHS) film electrogenerate...
Article
Full-text available
We demonstrate herein a newly developed photoelectrochemical immunosensor for the determination of anti-cholera toxin antibody by using a photosensitive biotinylated polypyrrole film. The latter was generated by electro-oxidation of a biotinylated tris(bipyridyl) ruthenium(II) complex bearing pyrrole groups. The photoexcitation of this modified ele...
Article
In order to determine the best kind of matrix that enables preservation of the enzymatic and electro-enzymatic activity of immobilized enzyme and provides accessibility towards substrate, various host materials (electropolymerized polypyrrole films, alginate polysaccharide, biocompatible synthetic latex and inorganic clays (laponite and layered dou...
Article
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This article explores the possibilities of using Sb doped SnO2 thin films as potential electrode substrates for biosensors. Two series of electrodes were modified with electrogenerated functionalised polymeric films. The polymers investigated were biotinylated poly(ruthenium pyrrole) and poly(pyrrole benzophenone). AFM studies show polymer morpholo...
Article
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Direct detection of peanut agglutinin/lactose interactions was realized by an electrochemical approach based on a polypyrrole coated electrode displaying pendant carbohydrates.
Article
This contribution reports, for the first time, the synthesis and electropolymerization of a pyrrole N-substituted by a nitrilotriacetic acid acting as a chelating center of Cu2+. A step-by-step approach for protein immobilization was developed via the successive coordination of Cu2+ and histidine-tagged proteins. The self-assembly of histidine-tagg...
Article
We developed a novel copolymer modified amperometric immunosensor for the detection of cholera antitoxin (anti-CT), by the electropolymerization of pyrrole-biotin and pyrrole-lactitobionamide monomers on platinum or glassy carbon electrodes. In the detection of cholera antitoxin we have used three enzymatic marker detection systems based on HRP-lab...
Article
The synthesis of a ruthenium (II) tris(bipyridyl) complex and its electropolymerization providing a biotinylated and photosensitive polymer were reported. The anchoring of the Cholera toxin onto this polypyrrolic film was carried out via affinity interactions based on avidin-biotin bridges between the polymer and the toxin. The photoelectrochemical...
Article
A biotinylated photosensitive polymer was electrogenerated from on a ruthenium complex bearing biotin and pyrrole groups; the resulting polypyrrolic film allowed the bioaffine immobilisation of avidin and biotinylated cholera toxin and the photoelectrochemical detection of the corresponding antibody.
Article
The construction of amperometric immunosensors to cholera antitoxin immunoglobulins were shown to have improved sensitivity when the cholera toxin B subunit biorecognition entity was linked to an electrogenerated biotinylated polypyrrole film copolymerized with pyrrole-lactobionamide monomer. The copolymer exhibits greater film permeability than bi...
Article
The functionalization of an oligonucleotide by flavin and deazaflavin derivatives allowed an amperometric determination of the hybridization process through the disappearance of the electroactivity of the free oligonucleotide and the appearance of a new electrochemical signal characteristic of the resulting duplex.
Article
The bioaffine immobilization of several avidin layers on an electrode modified by a biotinylated polymer was accomplished by the first biotinylated redox bridge consisted of a tris(bipyridyl)iron(II) complex bearing six pre-oriented biotin groups.
Article
We describe herein, the electrochemical properties of an hydroporphyrin of Ni(II) substituted by two pyrrole groups in organic media (THF and CH2Cl2). In the anodic region it has been shown that the electrochemical behaviour of this complex investigated by cyclic voltammetry and coulometry coupled with UV–visible spectroscopy is strongly dependent...
Article
The electrochemical immobilization of salicylate hydroxylase (SH) within poly(amphiphilic pyrrole ammonium) films electrogenerated on carbon electrode is described. The amperometric determination of salicylate is carried out at 0.4 V (vs. Ag/AgCl) via the oxidation of the enzymically generated catechol. The detection limit and the sensitivity of th...
Article
The functionalization of microelectrodes has been carried out by oxidative polymerization of a biotinyl pyrrole monomer in acetonitrile. The resulting biotinylated polymer was used for the simple and reagentless anchoring of avidin and biotinylated enzymes via avidin-biotin bridges. Thus, the immobilization of five layers of glucose oxidase was per...
Article
New Cu (II), Mn (III) and Fe (III) deuteroporphyrins functionalized by two electropolymerizable pyrrole groups have been synthesized. The electrooxidation of these metallodeuteroporphyrins has allowed the formation of conducting polypyrrole films exhibiting electrochemical behavior identical to that of the corresponding monomers. In addition, the e...
Article
The concept and potentialities of two original electrochemical procedures of enzyme immobilization based on functionalized polypyrroles are described. A two-step method consists of the adsorption of aqueous amphiphilic pyrrole monomerenzyme mixtures on an electrode surface followed by electropolymerization of the adsorbed amphiphilic monomers. Exam...
Article
A new biotin derivative functionalized by a pyrrole group has been synthesized. This functionalization has allowed the preparation of the first example of a poly(pyrrole-biotin) film by oxidative polymerization. In addition, the successful copolymerization of a polypyridinyl complex of ruthenium(II) containing pyrrole groups with the biotin derivat...
Article
The electropolymerization of an alkaline phosphatase (AP)−amphiphilic pyrrole ammonium mixture previously adsorbed on the electrode surface provides efficient entrapment of AP in a polypyrrole film. The resulting biosensor was applied to the detection of phenyl phosphate via amperometric oxidation at 0.6 V vs SCE of the enzymically generated phenol...
Article
A novel catalytic material (mesoporous TiO2 films) for the cathodic detection of hydrogen peroxide in air-saturated aqueous solution is described. The immobilization of glucose oxidase (GOD) as enzyme model onto the TiO2 electrode provides an efficient biosensor for the amperometric detection of glucose at -0.15 V (vs. SCE). GOD molecules were immo...
Article
Full-text available
Ce travail de thèse est consacré au développement de biocapteurs électrochimiques en utilisant des argiles ou des hydroxydes doubles lamellaires (HDL), riches en fer, comme matrices d'immobilisation de l'hémoglobine (Hb). Le but était de mettre en évidence la contribution des propriétés redox de ces matériaux dans l'amélioration des performances de...

Citations

... Recently, we have demonstrated that a deposit of a thin layer of carbon nanotube (CNT) on a non-conductive support could play the role of an electrode while offering good permeation to low molecular weight compounds in an aqueous medium [7]. Taking into account this property, we report here the original creation of a hollow planar bioelectrode of very low thickness and large surface area containing the enzyme in powder form. ...
... Several studies have reported the use of voltammetric methods for virus detection, including Palomar et al, who prepared homemade electrodes modified with a multiwalled carbon nanotube composite material for the detection of DENV 2 NS1. The DPV mode was chosen to analyze faradaic currents originating from the transfer electrons between the redox probe and the electrodes with and without the presence of 2 NS1 [124]. Liv et al also developed a voltammetric biosensor to detect SARS-CoV-2). ...
... At present, a multitude of different techniques have been successfully employed for the determination of DA, for example, high-performance liquid chromatography, fluorescence spectrophotometry, colorimetry, chemiluminescence analysis, capillary electrophoresis, mass-spectroscopy, surface-enhanced Raman scattering spectroscopy, electrochemical method, molecularly imprinted, photoelectrochemical analysis, and enzyme linkage [5][6][7][8][9][10][11][12][13][14][15]. ...
... Other processes first anodise the silicon wafer over a certain thickness and area, before opening the membranes from the backside. This can be achieved using dry etching techniques like Deep Reactive Ion Etching (DRIE) [88,17,19,79,36,89], as shown in Fig. 6,(c). For this type of process, the oxidation of the PSi prior to the etching is desirable, as this may reduce the etch rate of the porous matrix and create a more uniform membrane. ...
... When using an immunosensor to detect antibodies in plasma, the sensitivity tends to be slightly lower than using buffer due to a large number of interferers. Despite this, the use of the EIS technique makes it possible to achieve greater sensitivity than other techniques, also presenting greater simplicity and speed of analysis [110]. ...
... These systems were modified with polypyrrole-nitrilotriacetic acid (poly(pyrrole-NTA)) and Cu (II) complex to produce sensor devices. With great sensitivity and easy reproducibility, the cholera sensor showed a promising linear detection range from 10 −13 -10 −5 g mL −1 with a LOD of 10 −13 g mL −1 , which could be a potential sensing platform to detect cholera in the environment [229]. ...
... The cryo-electron microscopy (EM) results for pili fibers demonstrated that the hexaheme cytochrome OmcS formed stacks within distances of ~3.5-6 Å for the neighboring molecules, where close proximity was appropriate for electron hopping . The in vitro attempt created conductive protein nanowires through the integration of a prion domain HET-s to assemble into fibers and a redox protein rubredoxin (Rd) for electron hopping between the reduced and oxidized states (Altamura et al., 2017). The recombinant Rd-HET-s proteins were found to form the fiber structures and facilitate electron transfer through the Fe 2+ /Fe 3+ center in Rd. ...
... Siqueira de Oliveira et al. 14 reported on platinum and glassy carbon electrodes modified with uranyl Schiff base films 14 or carbon paste electrodes modified with salen complexes 15 that allowed the detection of cocaine in the low µM range with no interferences from lidocaine or pro-2 caine. Modification of the electrode surface with aptamers has also been widely applied in order to provide selectivity towards cocaine detection [16][17][18][19] . However, these modifications can be timeconsuming and costly. ...
... The electrolysis charge was limited to 2.5 mC cm −2 to form a thin and compact polymer layer. [26] The cyclic voltammogram of the resulting electrode, transferred in a solution exempt of monomer, F 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 2105880 (3 of 6) www.advancedsciencenews.com © 2021 Wiley-VCH GmbH www.small-journal.com exhibits a reversible peak system at E 1/2 = 0.27 V, reflecting the electroactivity of the polypyrrolic skeleton (Figure 3). ...
... In the voltammetric mode, a static potential is applied continuously to the sensing electrode and the current response measured by adding target analyte; the direction of the flow of electrons depends upon the property of aptamer towards the target [121][122][123][124]. The main drawback with the voltammetric aptasensors is the regeneration of the activity of the electrode between measurements, so researchers are focusing on lowcost mass production of single-time-use disposable screenprinted electrodes (SPEs) to overcome this problem [125][126][127]. ...