Chaminda Jayampath Seneviratne's research while affiliated with The University of Queensland and other places

Publications (35)

Article
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Objective: This prospective clinical study aim was to analyze the effect of the probiotic Lactobacillus reuteri Prodentis lozenges on salivary microbiome of subjects wearing fixed orthodontic appliances. Methods: Saliva samples were collected prior to consumption and 14th-day post probiotic lozenges consumption (n=40, age 18-23). Oral hygiene in...
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Periodontal diseases are initiated by the shift from microbe-host symbiosis to dysbiosis, and the disrupted host response predominantly contributes to tissue destruction. This study investigated whether and to what extent human oral keratinocytes (HOKs) challenged by a periodontal commensal or pathogen could differentially affect the chemotactic ac...
Article
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Objectives The objectives of the study were to investigate the in vitro and in vivo effects of various removable orthodontic retainer materials on clinical and microbiological parameters, specifically on saliva characteristics, oral hygiene, and salivary levels of Streptococcus mutans ( S. mutans ). Materials and Methods This study consists of two...
Article
Aim The present study aimed to investigate the salivary proteome profiles of pregnant women with gingivitis (PG) or without (HP) gingivitis and non-pregnant healthy controls (HC) by employing iTRAQ-based proteomics. Materials and methods Saliva samples were collected from 30 Chinese women comprising 10 subjects in each of the three groups (PG, HP,...
Article
Probiotics are thought to be beneficial microbes that influence health-related outcomes through host immunomodulation and modulation of the bacteriome. Its reported success in the treatment of gastrointestinal disorders has led to further research on its potential applicability within the dental field due to similarities such as a polymicrobial aet...
Article
The development of a successful vaccine against severe acute respiratory syndrome coronavirus‐2 (SARS‐CoV‐2), the agent of coronavirus disease 2019 (COVID‐19), in an unmatched period of ten months, is a tribute to human ingenuity in the face of a vicious pandemic. A return to pre‐pandemic `normalcy` depends on the successful delivery of the vaccine...
Article
Purpose Early pregnancy loss (EPL) is one of the most common complications encountered in clinical practice. As most of EPLs occur relatively early on during pregnancy, they are often misunderstood as an expected menstrual cycle. Thus, it is essential to investigate the diagnostic biomarkers for monitoring pregnancy loss for continuous non‐invasive...
Article
Pregnancy gingivitis peaks during mid-pregnancy and resolves transiently towards the postpartum period. However, the role of maternal immune response in orchestrating gingival inflammation has not yet been fully understood. Hence, in this study, we examined the salivary protein profile during the three trimesters of pregnancy, in context to pregnan...
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Halitosis or oral malodor is one of the most common reasons for the patients' visit to the dental clinic, ranking behind only dental caries and periodontitis. In the present times, where social and professional communications are becoming unavoidable, halitosis has become a concern of growing importance. Oral malodor mostly develops due to the putr...
Article
Objectives The dysbiotic oral microbiome plays a key role in the pathogenesis of caries in children. Topical application of casein phosphopeptide–amorphous calcium phosphate containing fluoride (CPP-ACP/F) is an effective treatment modality for children with caries (CC). Hitherto the mechanism by which CPP-ACP/F modules the oral microbiome in CC ha...
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Objectives Candida albicanscolonizes biomaterial surfaces and are highly resistant to therapeutics. Graphene nanocoating on titanium compromises initial biofilm formation. However, its sustained antibiofilm potential is unknown. The objective of this study was to investigate the potential of graphene nanocoating to decrease long-term fungal biofilm...
Article
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Candida albicans is a commensal polymorphic and opportunistic fungus, which usually resides as a small community in the oral cavities of a majority of humans. The latter eco-system presents this yeast varied opportunities for mutualistic interactions with other cohabitant oral bacteria, that synergizes its persistence and pathogenicity. Collectivel...
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The emergence of the highly infectious novel coronavirus SARS-CoV-2 has led to a global COVID-19 pandemic. Since the outbreak of COVID-19, worldwide healthcare systems have been severely challenged. The rapid and explosive surge of positive cases has significantly increased the demand for medical care. Herein we provide a perspective on the role de...
Article
Introduction Previous studies have largely explored the microbial composition and pathogenesis of pregnancy gingivitis. However, the patterns of microbial colonization during pregnancy in the absence of pregnancy gingivitis have rarely been studied. Characterization of the oral microbiome in pregnant women with healthy gingiva is an important initi...
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Streptococcus mutans is the principal biofilm forming oral pathogen associated with dental caries. Studies have shown that Candida albicans, a commensal oral fungus is capable of forming pathogenic mixed-species biofilms with S. mutans. The treatment of bacterial and fungal infections using conventional antimicrobial agents has become challenging d...
Article
Objective Among the preventive and therapeutic options available for dental caries, sugar alcohols (xylitol and sorbitol) have been widely promoted as oral healthcare products due to its perceived anticariogenic effect. However, the therapeutic efficacy of these sugar alcohols against Streptococcus mutans and Candida albicans in a sucrose supplemen...
Article
In the past decade, there has been a tremendous increase in studies on the link between oral microbiome and systemic diseases. However, variations in study design and confounding variables across studies often lead to inconsistent observations. In this narrative review, we have discussed the potential influence of study design and confounding varia...
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Candida albicans is a major fungal pathogen, accounting for approximately 15% of healthcare infections with associated mortality as high as 40% in the case of systemic candidiasis. Antifungal agents for C. albicans infections are limited, and rising resistance is an inevitable problem. Therefore, understanding the mechanism behind antifungal respon...
Article
Background Machine learning (ML) is powerful tool that can identify and classify patterns from large quantities of cancer genomic data that may lead to the discovery of new biomarkers, new drug targets, and a better understanding of important cancer genes. The aim of this systematic review was to evaluate the existing literature and assess the appl...
Article
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It is well known that pregnancy is under the constant influence of hormonal, metabolic and immunological factors and this may impact the oral microbiota toward pregnancy gingivitis. However, it is still not clear how the oral microbial dysbiosis can modulate oral diseases as oral microbiome during pregnancy is very poorly characterized. In addition...
Presentation
TITLE: Comparative in vivo nephrotoxicity of a novel antifungal agent Objectives: Candida albicans is the major fungal species associated with superficial mucosal infections such as oral candidiasis as well as systemic mycoses with high morbidity and mortality. On top of the rising drug resistance, currently available antifungal agents have signifi...
Article
Full-text available
Objective: To examine the correlations among oral health knowledge, attitude, practices and oral disease among pregnant Chinese women in Singapore. Methods: A descriptive correlational study was conducted in pregnant Chinese women in Singapore. A questionnaire was used to collect data of oral health knowledge, attitude and practices. Plaque index...

Citations

... Probiotic supplementation inhibits Treponema_2 in pig caecal digesta [12]. L. reuteri substantially reduced the abundance of Treponema sp. in the human mouth [58]. Similar to Xu et al., the abundance of Prevotellaceae_NK3B31_group was substantially decreased in pigs administered compound probiotic diets [59]. ...
... Probiotics are a potential means of managing halitosis due to its mechanism of action. These include competitive exclusion of bacteria, immune modulation, and production of antimicrobial substances [8][9][10]. After the first case series on the effect of probiotics on reducing halitosis was reported only as case series [11,12], an increasing number of publications have been observed, but there are currently no specific recommendations on the administration regimen of probiotics. ...
... So far, several vaccines for COVID-19 have been developed by various pharmaceutical companies. Some of them have been authorized by the US Food and Drug Administration (FDA) and are widely used in many countries which had a major impact on reducing mortality from the disease [4][5][6]. However, the epidemic will probably continue until the global launch of safe and effective vaccines to provide herd immunity. ...
... This is because biofilms can delay or prevent the penetration of antibiotics [39], acquire resistance via horizontal gene transfer [40], and use multidrug efflux pumps to pump antibiotic agents out of the maturing biofilms and into the extracellular matrix [41]. In addition, biofilms can activate the innate immune system via secretion of C-di-NMPs, which induce an immune response through STING and subsequently activate type 1 IFNs [42]. ...
... Halitozisle ilgili çalışmaların sonuçları incelendiğinde; halitozise sebep olan uçucu bileşiklerin üretiminin tek bir (Gram negatif anaerobik) bakteri ''varlığı''ndan ziyade birden fazla türün var olduğu bir bakteriyel ağ ve onun içerisinde gerçekleşen aktivitelerden kaynaklı olduğu düşünülebilir. (67,70,71,76,79). Gram pozitif bakterilerden Streptococcus salivarius'un IOH'li bireylerde düşük miktarda bulunmasına rağmen, tükürük proteinlerini (glikoproteinler) deglikozide ederek, onları Gram negatif bakterilerce (P. ...
... Microbiome profiling of the saliva samples was performed using a previously established protocol with some modification [10]. In brief, DNA was extracted from the samples using the QIAamp® DNA Mini Kit (Qiagen, Hilden, Germany) per the manufacturer's protocol. ...
... When coated on titanium, inhibition by scattering of cells led to inability of the microorganism to form mature biological films (Banerjee et al., 2010), thus giving prospects as a potential coating on implants and medical devices. Coatings of this nature can prevent microbial proliferation without the administration of antibiotics (Agarwalla et al., 2021). Surface roughness varies with the duration of deposition but studies have shown that it has negligible correlation to biofilm formation. ...
... In contrary, in the same work, the downregulation of BCR1 and EFG1 was noticed under the same conditions for C. albicans ATCC 18804 interaction with S. sanguinis (do Rosaŕio Palma et al., 2019). Moreover, the analyses of changes in the proteome of mature 48h biofilm formed by C. albicans standard laboratory strain SC5314 after its 2-h exposure to 10 8 heat-killed bacteria revealed the reduction in the expression of Efg1 protein upon the contact with S. mitis and S. sanguinis, as well as with P. gingivalis, F. nucleatum, and A. actinomycetemcomitans (Truong et al., 2020). ...
... The role of dentists and dental students in the fight against COVID-19 is of utmost value [12,13], not only in terms of safe provision of oral/dental care during the pandemic, but also in educating and motivating the public to accept COVID-19 vaccines and obeying preventive measures. The current survey was carried out in this context to shed light on dentists and dental students' acceptance of COVID-19 vaccines and related determinants of vaccine acceptance. ...
... Several studies have conclusively established that sugar alcohols, such as xylitol, inhibit the growth of pathogenic oral bacteria, including cariogenic microorganisms in humans [17,18,19]. Sugar alcohols have bacteriostatic effects on a variety of bacteria [20,21], and they are effectively and widely used in daily oral care in humans [22,23]. ...