Camilo Ernesto López Carrascal's research while affiliated with National University of Colombia and other places

Publications (25)

Article
La bacteriosis vascular de la yuca, causada por la bacteria gram negativa Xanthomonas phaseoli pv. manihotis (Xpm), anteriormente conocida como Xanthomonas axonopodis pv. manihotis, es la principal enfermedad bacteriana que compromete su producción. Con la meta de generar una resistencia durable y de amplio espectro a la bacteriosis es posible expl...
Article
Uno de los retos que encara la humanidad es asegurar la alimentación y la adecuada nutrición para los cerca de ocho billones de habitantes del planeta. Las raíces de yuca constituyen la cuarta fuente más importante de calorías para la población humana siendo uno de los pilares de la seguridad alimentaria. Las raíces de yuca no poseen atributos nutr...
Article
Cassava (Manihot esculenta Crantz) is an essential crop in tropical regions providing food for about one billion people around the world. The most important bacterial disease is cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam). Recently, a resistance gene called RRXAM1 was identified, which codes for a protein tha...
Article
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La yuca (Manihot esculenta) representa el pilar de la seguridad alimentaria para cerca de mil millones de personas, principalmente en las zonas tropicales. Uno de los factores limitantes de la producción de yuca es la bacteriosis vascular causada por la bacteria Xanthomonas axonopodis pv. manihotis (Xam). Recientemente se identificó el gen RXam1 el...
Article
Once pathogens are perceived by plants a signal transduction pathway is activated leading to the induction of transcription factors, which in turn reprogram the host gene expression including the transcription of PR (Pathogenesis-Related) genes. The PR proteins are well known for their antimicrobial activity and for contributing to arrest the invas...
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Posterior al reconocimiento de agentes patógenos las plantas activan una serie de cascadas de señalización que culminan con la activación de factores de transcripción. Esto genera una concomitante reprogramación de la expresión génica que incluye la activación de la transcripción de los genes PR (relacionados con patogenicidad). Las proteínas PR so...
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La expresión transitoria es una métodología ampliamente utilizada para el estudio de genes. Sin embargo, hasta la fecha no existe un reporte en donde se utilice esta técnica en hojas de yuca de plantas adultas. Por esta razón este trabajo se centró en la determinación de algunos parámetros críticos para la expresión transitoria del gen GUS en yuca...
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Main conclusion: The overexpression of RXam1 leads to a reduction in bacterial growth of XamCIO136, suggesting that RXam1 might be implicated in strain-specific resistance. Cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam) is a prevalent disease in all regions, where cassava is cultivated. CBB is a foliar and vasc...
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One of the most challenging questions in plant breeding and molecular plant pathology research is what are the genetic and molecular bases of quantitative disease resistance (QDR)?. The scarce knowledge of how this type of resistance works has hindered plant breeders to fully take advantage of it. To overcome these obstacles new methodologies for t...
Article
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Cassava, Manihot esculenta Crantz, has been positioned as one of the most promising crops world-wide representing the staple security for more than one billion people mainly in poor countries. Cassava production is constantly threatened by several diseases, including cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam...
Article
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La yuca, Manihot esculenta Crantz, representa la principal fuente de alimento para cerca de 1000 millones de personas. La producción de yuca se ve afectada por diversas enfermedades, una de las más serias es la bacteriosis vascular (CBB) causada por Xanthomonas axonopodis pv. manihotis (Xam). En este estudio se realizó un análisis de loci de rasgos...
Article
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Las nuevas tecnologías para la edición de genomas, como los TALEN y el sistema CRISPR/Cas9, representan una gran oportunidad para mejorar características deseables en diferentes organismos. Los TALEN son el resultado del acoplamiento de nucleasas a los TALE (Transcription Activator-Like Effectors), los cuales son efectores naturales de gran importa...
Article
Infección de callo embriogénico friable de yuca con Xanthomonas axonopodis pv. manihotis (Xam) Cassava friable embryogenic calli infection with Xanthomonas axonopodis pv. manihotis (Xam) Resumen Las nuevas tecnologías para la edición de genomas, como los TALEN y el sistema CRISPR/Cas9, representan una gran oportunidad para mejorar características d...
Article
Full-text available
p class="ecxmsonormal"> Xanthomonas axonopodis pv. manihotis ( Xam ) is the causal agent of cassava bacterial blight (CBB), a major disease for cassava crops in South America and Africa. Until now the development of the disease is measured via AUDPC (Area Under Disease Progress Curve) but no reliable quantitative methods are available probably due...
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p>Las raíces almacenadoras de yuca representan una fuente importante de almidón. La ruta metabólica del almidón ha sido reconstruida recientemente en yuca gracias a la liberación de la secuencia completa de su genoma. En este estudio se evaluó la expresión de los genes que codifican para las enzimas Pululanasa, Isoamilasa, α-amilasa, Enzima Desprop...
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La yuca (Manihot esculenta Crantz) presenta un alto contenido de almidón en sus raíces almacenadoras, por lo que es una potencial materia prima para el mercado de agro-combustibles. En este proyecto se aprovecha la estrategia del silenciamiento génico mediado por ARN de interferencia (ARNi), para silenciar algunos de los genes de la ruta de biosínt...
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The evolutionist theory proposed by Darwin is one of the fundamental pillars in biology. Darwin's theory was solidified with the Modern synthesis of evolutionary biology thanks to the rediscovery of Mendel's work, which laid the genetic basis of heredity. In recent years, great progress has been acquired in the sequencing and analyses of complete g...
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La yuca (Manihot esculenta) constituye la base de la alimentación para más de 1.000 millones de personas en el mundo, consolidándose como el cuarto cultivo más importante en el mundo después del arroz, el maíz y el trigo. La yuca es considerada como un cultivo relativamente tolerante a condiciones de estrés abiótico y biótico, sin embargo estas car...
Article
Cassava is one of the most important crops for global food security and provides food and livelihood for 600 million people in the developing world. It is also good source of starch, with levels between 73.7 y 84.9% of total dry weight in roots (FAO, 2007). Cassava starch can be used in a wide range of industries (textile, cosmetic, nourishing, etc...
Article
Full-text available
Cassava is one of the most important crops for global food security and provides food and livelihood for 600 million people in the developing world. It is also good source of starch, with levels between 73.7 y 84.9% of total dry weight in roots (FAO, 2007). Cassava starch can be used in a wide range of industries (textile, cosmetic, nourishing, etc...
Article
Full-text available
Cassava (Manihot esculenta Crantz, Euphorbiaceae) is the fourth food crop used as an important energy source for human population worldwide. Cassava Bacterial Blight (CBB) is the most important disease of this crop. CBB is caused by the pathogenic bacterium Xanthomonas axonopodis pv. manihotis (Xam). Plants have developed sophisticated mechanisms t...

Citations

... 5th edition [6]). This method highly quantitative and can reveal subtle differences in virulence between related pathogen strains or mutants (Bart [7], Cohn and Bart [8], Diaz [9]). However, this assay probes only one part of the disease cycle and provides limited insight into pathogen spread, plant symptoms or defense responses. ...
... R-gene-mediated resistance results in distinguishable phenotypic classes (resistant or susceptible) following the molecular interaction of the host plant and pathogen genes. However, QR exhibits a spectrum of phenotypic values between these categories, which the two-tier plant immunity model does not account for (Vasquez et al. 2018). Recently, an alternative model to explain plant immunity that encompasses both R-gene and QR-mediated resistance, the invasion model of plant immunity, has been proposed (Cook et al. 2015). ...
... However, it has been observed that CBB can result in up to 100% loss in productivity during high humidity and moisture, conditions that allow maximum severity (López and Bernal 2012). Efforts to map the sources of CBB resistance in different cultivars have been carried out mainly through QTLs (López et al., 2007;López and Bernal, 2012;Soto Sedano et al., 2017). Due to high levels of environmental influence on CBB severity, no major QTLs have been mapped yet. ...
... Two new QTL associated with CIO151 and CIO121 strains explained 61.6% and 21%, respectively (López et al. 2007). More recently, two further QTL were detected, explaining 10.9% and 12.6% of phenotypic variance (Soto Sedano et al. 2017). ...
... Leaf inoculation may be questionable for resistance phenotyping because discrepancies were observed between leaf lesion measurements and scoring on stem inoculation (Muñoz-Bodnar et al., 2015;Restrepo et al., 2000a). However, others have successfully detected symptom differences when infiltrating leaves of susceptible and resistant cultivars at low densities (from 10 2 to 10 5 cfu/ml; Flood et al., 1995;Wydra et al., 2004Wydra et al., , 2007. ...