C Mirre's research while affiliated with Institut de France, Académie des sciences and other places
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Publications (36)
To understand the molecular mechanisms underlying sorting of apical and basolateral membrane components in human intestinal epithelial cells, we have cloned the human homolog of rat syntaxin 3 and looked for its subcellular localization. Endogenous human syntaxin 3 was found to be localized at the apical membrane of colon epithelial and Caco-2 cell...
In this study we analyzed the relationship between detergent-resistant microdomains and caveolae in Caco-2 cells. Caveolin was not detected on Western blots or Northern blots or by immunoprecipitation in these cells, in contrast to A 431 cells. Triton X-100-resistant membranes from Caco-2 and A 431 cells showed the same morphological aspect by elec...
In this study, we have investigated the possibility that glycosyl-phosphatidylinositol (GPI)-anchored proteins form insoluble membrane complexes in Caco-2 cells and that transmembrane proteins are associated with these complexes. GPI-anchored proteins were mainly resistant to Triton X-100 (TX-100) extraction at 4 degrees C but fully soluble in n-oc...
By means of immunocytochemistry with a battery of specific antibodies, we describe the expression of the collagen IV chain produced by the gene DCg1 during the two phases of Drosophila neurogenesis. DgC1 was not expressed in neuronal tissues as shown by in situ hybridization, but the onset of its expression in cells of mesodermal origin was concomi...
We have developed and characterized a battery of specific polyclonal antibodies directed against specific portions of the alpha-chain of collagen type IV synthesized in Drosophila by the gene DCg1. Here, we describe the use of these antibodies together with in situ hybridization experiments in an attempt to study the expression and localization of...
We report the purification and characterization of three sequence-specific polyclonal antibodies raised against specific portions of the Drosophila αIV collagen chain produced from the gene DCg1. These antibodies were used for immunolocalization experiments on tissue sections from embryonic organogenesis stages (13–17) and first larval stages. This...
We have examined directly the expression of one collagen gene (DCg1) during Drosophila melanogaster embryogenesis by means of in situ hybridization. Transcripts of this gene, which were demonstrated to encode a basement membrane type IV collagen chain, began to accumulate specifically in mesodermal derivatives at stages 12-13 of embryogenesis, and...
Type IV collagen, a major structural component of basement membrane, has been characterized only in vertebrates. It is unique among the collagenous proteins in that it forms specific lattice networks by end-to-end interactions. In particular, in mammals the C-terminal noncollagenous domain (NCl) of collagen IV was shown to be one of the major cross...
We report a direct examination of the expression of one collagen gene (DCg1) during Drosophila melanogaster metamorphosis, based on data from in situ hybridization. The transcripts of this gene, thought to encode a basement membrane type IV collagen, are mainly accumulated during ecdysis in wandering haemocytes. Our results demonstrate that haemocy...
Based on data from developmental RNA profiles and in situ hybridization, we report a direct examination of the expression of one collagen gene (Dcg1) during drosophila melanogaster life cycle. These studies show, for the first time, that the expression of a collagen gene is both differential and tissue-specific during the course of development. Mor...
We have used a cloned chicken collagen cDNA sequence to help identify hypothetic members of the collagen gene family from Drosophila melanogaster. Several experimental evidences have been obtained which indicate that the Drosophila genome contains numerous collagen-like sequences. We have characterized in more detail ten distinct DNA sequences that...
The localization, structure, and activity of the nucleolus-organizers (NORs) were studied during spermiogenesis in the mouse by light and electron microscopy procedures including NOR-silver-staining and actinomycin D treatment. After the two meiotic divisions the NORs resume their activity during the Golgi phase of spermatid differentiation (steps...
In embryonic cell-line derivative KCo of Drosophila melanogaster, the nucleolus, like most nucleoli, contains a small proportion of ribosomal DNA (1-2% of the total nucleolar DNA). The ribosomal DNA is virtually the only active gene set in the nucleolus and is found among long stretches of inactive supercoiled heterochromatic segments. We have demo...
In mouse oocyte during meiotic prophase I, rRNA synthesis increases from midpachytene (onset of nucleologenesis) to the diplotene stage (both 4C DNA containing). During these stages the nucleoli undergone morphological changes from the ring-shaped structure to the nucleolonemal type. The results we obtained by 3-D reconstruction after serial sectio...
In adult Sertoli cells of most strains of mice, all the centromeric heterochromatin is condensed in two chromocenters, one on each side of a single, large nucleolus. In a random-bred Swiss OF-1 strain, however, the nucleus has a different structural organization. Part of the heterochromatin is seen as chromocenters in contact with the nucleolus; th...
During meiotic prophase I the nucleolus of the mouse oocyte assumes a reticulate structure of 'nucleolonema' type. This change coincides with the appearance of several secondary fibrillar centres. The number of these centres at diplotene (97-113), largely exceeds that of nucleolar organizers (4c DNA = 20 NORs). The quantitative analysis of autoradi...
Classical electron-microscopic techniques (enzymic digestion, EDTA regressive staining) allied with autoradiographic studies after [3H]uridine incorporation or after RNA synthesis initiated by an exogeneous RNA polymerase in the presence of tritiated GTP, enabled us to describe the fine structure and activity of the nucleolus in an established Dros...
In mouse testis, the diploid Sertoli cell displays one large nucleolus flanked symmetrically by two heterochromatic masses. The hybridization in situ with [3H]rRNA confirmed that the ribosomal cistrons are localized with in the central nucleolar mass. At the ultrastructural level this nucleolar mass appears to be reticulated and contains numerous f...
The emergence of newly formed nucleoli and their development have been studied in mouse oocytes from pachytene to diplotene stages. At mid-pachytene, the nucleolus first appears as a fibrillar centre surrounded by a layer of electron-dense fibrils and penetrated by chromatin fibres emanating from the secondary constriction region of the nucleolar b...
Selective silver staining demonstrated that autosomal bivalents containing transcriptively active nucleolar organizers associated with the sex vesicle during pachytene of mouse spermatocytes. Later in pachytene, the nucleolar organizers covered the portion of the sex vesicle furthest from the attachment to the nuclear envelope. Hybridization in sit...
Prophase I meiosis was studied in the human oocyte obtained from 16- to 24-week-old fetuses. Electron microscopy and silver stainihg showed that, at pachytene, the ribosomal genes belonging to several chromosomes are gathered in the same nucleolar fibrillar center, where they are embedded in an argyrophilic protein. The nucleolus showed spontaneous...
The structure, localization and activity of ribosomal genes were studied in quail, mouse and human oocytes. Meiotic prophase I is a favourable stage for these studies since the connections between the nucleoli and the chromosomes are analyzable by light and electron microscopy. In the newly-formed nucleolus at pachytene in the quail and mouse, the...
Use of the silver-NOR method to study the nucleolar organizers in human oocytes demonstrates that topographic and quantitative variations occur during meiotic prophase. In the oogonia nucleolus the nucleolar organizers are dispersed, whereas beginning at leptotene and throughout the remaining stages of meiotic prophase they occupy a marginal positi...
Nuclear triiodothyronine was visualized by light and electron microscope autoradiography of liver nuclei isolated after intraperitoneal injection of [125I] triiodothyronine in rats. The nuclear hormone, essentially bound to the putative nuclear triiodothyronine receptor, was found mostly associated with the border of condensed chromatin.
In the quail, the somatic ovarian cell and oocyte nucleoli are composed of a fibrillar center which is constantly surrounded by a layer of electron-opaque fibrils and a fibrillo-granular region. Enzymatic digestion using Pronase, RNase, and DNase demonstrated that the fibrillar center contains DNA and proteins. Incorporation of tritiated actinomyci...
The mouse oocyte is the site of nucleolar synthesis during pachytene. The chromosomes containing a nucleolar organizer are attached to the nuclear envelope by their paracentromeric heterochromatin, either alone or by taking part in the formation of a chromocentre. The nucleolus appears at the junction of the paracentromeric heterochromatin with the...
The localization, structure and activity of nuclear organizers were studied in quail and mouse oocytes during prophase I of meiosis using electron microscopy and in situ hybridization with tritium labelled 18S and 28S RNA. The newly synthesized nucleolus consists of a fibrillar centre surrounded by a layer of electron-opaque fibrils. DNP fibres wit...
The nucleolar organizers have been localized in the Japanese quail oocyte at pachytene and diplotene stages using hybridization in situ with rRNA. Autoradiography reveals that 8 microchromosomes, forming 4 bivalents at pachytene, contain ribosomal cistrons. The silver grains are located on the microchromosomes euchrpmatic segment, near the centrome...
The localization of nucleolar organizers in the quail oocyte was studied during meiotic prophase I.
During midpachytene, the oocyte nucleus contains chromocenters formed by association of the heterochromatic centromeric region of the microchromosomes. During late pachytene, the nucleolus always develops in strict relation to a chromosomal structure...
The localization of nucleolar organizers has been studied in the nuclei of the somatic cells of one-day-old quail ovaries with in situ hybridization techniques. In these cells the nucleolus apparatus has a chromocentre formed by heterochromatin and small nucleoli typified by cytochemical and ultrastructural characteristics, associated at the periph...
Right and left gonads from chick embryo of various ages (4, 5, 6, 12 and 13 1/2 days of incubation) were fixed in 3% glutaraldehyde, post fixed in 2% osmium tetroxide and embedded in Epon. Aspects of apical portions of the somatic cells in the germinal epithelium at 5 days have been mainly studied and compared to these observed in gonads at 4, 6, 1...
Citations
... InXenopus vitellogenic oocyte, the reticular pattern of the active NOR is reminiscent of the skein-like NOR from both classical compact chromosomal nucleoli (Couve & Esponda, 1982) and the nucleolonemal-like nucleoli (Mirre & Stahl, 1981). However, in Xenopus oocytes, the NOR does not show any fibrillar electrontranslucent centre, as expected from chromosomal NOR studies (Mirre & Stahl, 1976;Mirre & Knibiehler, 1981). Apparently, the NOR consists only of a dense fibrillar core with emerging strings, known to be the site of active transcription (Miller, 1969;Miller, Miller & Beatty, 1969). ...
... C'est en fin de pachytène que sa réorganisation et sa croissance reprennent. L'organisation du nucléole et l'étude des synthèses d'ARN et d'ADN ont fait l'objet de nombreuses descriptions ultrastructurales (rat : Schuchner, 1975 ;chat : Morato, 1965 ;champignons : Stockert et al., 1970 ;plantes : La Cour, 1975 ;souris et cailie : Mirre et Stahl, 1976homme : Très, 1975 ;Stahl et al., 1978Stahl et al., , 1980Hartung et al., 1979 ;Mirre et al., 1980). Cette évolution des composés nucléolaires est en relation avec les synthèses nucléaires. ...
... In a previous study we showed that the oocytes of birds and mammals represent particularly interesting material for studying ribosomal cistrons (Stahl et al., 1975a). In various species, de novo synthesis of nucleolar material occurs during one of the phases of meiotic prophase I. Accordingly, newly synthesized nucleoli _are formed during pachytene in the quail and the mouse Stahl, 1976,1978 ;. ...
... Hemocytes express several extracellular matrix (ECM) components such as the enzyme Peroxidasin (Nelson et al., 1994), basement membrane associated dSPARC (Martinek et al., 2002), and the proteoglycan MDP-1 (Hortsch et al., 1998). Hemocytes also produce structural components of the basement membrane such as LamininA (Kusche-Gullberg et al., 1992), and the two Collagen IV molecules identified in Drosophila: Cg25C and Viking (Knibiehler et al., 1987;Le Parco et al., 1989;Mirre et al., 1988;Yasothornsrikul et al., 1997). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/273860322918406-1442304797341_Q64/Yannick-Le-Parco.jpg)
... Number of spermatids per Sertoli cell (SC), based on the available literature, for different vertebrate groups. [6,15,16,18,34,35,37,38,45] Euchromatic with heavy [ 17, 19, 3 7] indentation of the nuclear membrane Tripartite structure that is [2,11,14,17,23,37] used as a constant for counting Resorption of the residual [5,11,12,21,24,27,30] body of spermatid cytoplasm, within -24 hours Associated with nucleus, [36,[40][41][42] junctions and bundles of spermatids for epithelial transport Sertoli-Sertoli cell [8,9,29] junctions make up the unique barrier (BTB) Basal endoplasmic [9,13,29,33,39] reticulum aligned with actin bundles at the plasma membranes between Sertoli cells. ...
... Embedded cells, such as fibroblast-like cells and muscle, may be the source but seem too few and widely dispersed to cover the large surface area of the entire hemal space. It has been suggested, at least in insects [62][63][64][65][66][67], that these matrices are deposited by the circulating hemocytes, which could also be the evolutionary source of the endothelia seen in closed circulatory systems [4,68,69]. If this can be shown for crustaceans, then the role of the hemocytes will be greatly expanded from their primary research focus on innate immune responses and wound healing [7,10,70]. ...
... The number of FC does not depend on the number of chromosomes with active NOR. The sizes of FC depend on the functional condition of a cell, and on the intensity of transcription of rDNA (Mirre and Knibiehler, 1984). The data of Buhmeida et al. (2000) and Derenzini et al. (2000) demonstrate that the size and function of the nucleolus is related to the proliferation rate of cancer tissue. ...
... The main category of such sequences is rDNA and centromeres (Table 1). However, rDNA is expelled from the NPB mass upon termination of transcription by RNA polymerase I [11,24] and should therefore suffer only very limited damage in the transcriptionally silent oocytes. When analysed, metaphase II oocytes that had been previously enucleated show morphologically intact and lesion-free chromosomes with well-defined pericentric chromatin, centromeres and telomeres, suggesting that chromosomes remain intact after enucleolation [23]. ...
... The ovary sections were then incubated overnight with anti-SYCP3 antibody (dilution, 1:100) at 37 °C, followed by incubation with Alexa Fluor 488-conjugated secondary antibodies for 1 h at 37 °C and 5 μ g/mL Hoechst 33342 for 5 min. SYCP3 staining was performed to identify chromosomal axial elements during meiotic prophase I. Zygotene, pachytene, diplotene, and dictyate stages of meiotic prophase I were distinguished based on the appearance of axial elements 48,49 . In all, 300 oocytes from 2-3 ovaries were imaged for each slide and were counted using TCS SP8 STED downright microscope (Leica). ...
... The location of NORs on microchromosomes in quail is a conserved avian feature (to date) (Barbosa et al. 2013). Knibiehler et al. (1977) found four nucleolar organizers in quail, all Comparative telomeric array organization among the Galliformes species under investigation. Mitotic metaphase chromosomes were hybridized with telomere-PNA probe (green) and counterstained with DAPI (blue) in order to study the telomeric patterns. ...
