Bruce Budowle’s research while affiliated with Radford University and other places

Tissue identification can be instrumental in reconstructing a crime scene but remains a challenging task in forensic investigations. Conventionally, identifying the presence of certain tissue from tissue mixture by predefined cell type markers in bulk fashion is challenging due to limitations in sensitivity and accuracy. In contrast, single-cell RNA sequencing (scRNA-Seq) is a promising technology that has the potential to enhance or even revolutionize tissue and cell type identification. In this study, we developed a high sensitive general purpose single cell annotation pipeline, scTissueID, to accurately evaluate the single cell profile quality and precisely determine the cell and tissue types based on scRNA profiles. By incorporating a crucial and unique reference cell quality differentiation phase of targeting only high confident cells as reference, scTissueID achieved better and consistent performance in determining cell and tissue types compared to 8 state-of-art single cell annotation pipelines and 6 widely adopted machine learning algorithms, as demonstrated through a large-scale and comprehensive comparison study using both forensic-relevant and Human Cell Atlas (HCA) data. We highlighted the significance of cell quality differentiation, a previously undervalued factor. Thus, this study offers a tool capable of accurately and efficiently identifying cell and tissue types, with broad applicability to forensic investigations and other biomedical research endeavors.

The U.S. Federal Aviation Administration (FAA) recently provided detailed instructions on how Aviation Medical Examiners (AMEs) should assess and evaluate pilots for post-traumatic stress disorder (PTSD). The European, Australian and International Civil Aviation Organization guidelines for the assessment of PTSD in aviation are general guidelines and do not address the unique and specific circumstances of a flight crew per se. The starting point of the U.S. FAA’s guidance is an already-established clinical PTSD diagnosis since it is known that PTSD compromises aviation safety and has been related to fatal aviation accidents. According to the FAA’s guidance, a PTSD assessment is undertaken based on whether the condition is symptomatic and medicated, or whether more than 2 years have elapsed since showing symptoms and receiving medication. The International Classification of Diseases (ICD) criteria for stress disorders have changed between versions ICD-10 and the soon-to-be-released ICD-11. The new ICD-11 criteria are discussed in this article in the context of aviation health. Additionally, PTSD, potentially caused by an incident of turbulence, is discussed in the context of aviation mental health. There are currently no published studies on turbulence-caused mental trauma. We have identified in this article potential factors which are related to pilots’ and cabin crew’s stressors in incidents of severe and extreme turbulence. Three recommendations are provided: (1) harmonize assessment practices of PTSD internationally; (2) healthcare professionals taking care of traumatized flight crew should have a follow-up guide that takes specific and local conditions into account, and ensures the identification of patients who require follow-up treatment as early as possible; and (3) aviation health care professionals should consider ICD-11 diagnostic criteria as the information may be more useful in the assessment and diagnosis of aviation-related trauma.

Investigations of aviation accidents are based on provisions of the International Civil Aviation Organization (ICAO) Annex 13 agreement. A safety recommendation may be addressed to international aviation organizations, such as the European Aviation Safety Agency (EASA) and the ICAO, by the Accident Investigation Agency of any country, following a safety investigation into an occurrence. This process also promotes learning from smaller aviation accidents internationally. This article highlights two relatively small aviation accidents in Finland from 2016 and 2021, respectively, both of which fall under general aviation and have led to significant aeromedical safety recommendations for the EASA and the ICAO. In the 2016 accident, a general aviation pilot, who had previously suffered heart attacks, suffered another heart attack and died shortly after landing. This incident led to a recommendation issued to EASA calling for additional training of aeromedical officers in aeromedical risk assessment on pre-existing health conditions. The 2021 accident involved an elderly general aviation pilot who became incapacitated due to health issues. The recommendations resulting from this investigation were brought further to the ICAO and EASA in order for them to emphasize the importance of the age of a pilot in current aviation health assessment guidelines.

KinSNP® v1.0, a software tool for human identification, has been widely used to measure IBD segment sharing between individuals using dense SNP data. Herein, the tool was validated using simulated pedigree data (up to 9th degree relationships) from five diverse populations from the 1000 Genomes Project. Performance was further tested under conditions of simulated genotyping errors and allele or locus dropout. KinSNP data were benchmarked with IBIS, Ped-sim, and known ranges of centimorgan sharing. The calculated values from KinSNP aligned closely with IBIS and Ped-sim benchmarks, and accuracy was maintained with up to 75% simulated missing data. However, even slight increases in simulated sequence error rates negatively impacted performance. This study supports that KinSNP is a reliable solution for IBD-based analyses in forensic contexts.

The 4th Forensic DNA Symposium in Africa underscored the critical role of regional collaboration in advancing forensic sciences, with a particular focus on forensic DNA examinations, databases, and humanitarian initiatives. The symposium aimed to assess the current forensic DNA capabilities across African countries and develop strategies to expand and better utilize DNA platforms. Key findings from the symposium highlight the necessity for enhanced cooperation among African nations to build robust forensic DNA databases and improve data-sharing mechanisms. The symposium also identified significant gaps in current capabilities and the need to develop legal frameworks, infrastructure, and expertise to support forensic initiatives. Moving forward, these findings suggest a strategic focus on capacity building, establishing standardized procedures, and implementing sustainable forensic practices across the continent. Champions were nominated by attending delegates to lead their respective countries in the implementation of these strategies, marking a critical step towards strengthening forensic science in Africa and addressing the pressing challenges related to crime and humanitarian efforts.

There is growing concern that the severe respiratory disease in birds (avian influenza or ‘bird flu’) caused by the H5N1 influenza virus, might potentially spread more widely to humans and cause a pandemic. Here we discuss clinical issues related to human infections by the highly pathogenic H5N1 subtype of the avian influenza A virus and make a clinical comparison with recent information obtained from studies of SARS-CoV-2 infection. Firstly, we consider the potential increase in cardiovascular events in humans infected with the H5N1 virus. Like SARS-CoV-2 infection, H5N1 infection may result in endothelial dysfunction and the associated procoagulant and prothrombotic state, and via this mechanism, the infection can potentially increase cardiovascular morbidity, especially in vulnerable individuals with pre-existing cardiovascular disease. Secondly, we discuss the potential beneficial role of statin use, both in the prophylaxis and the treatment of individuals with influenza A(H5N1), as was found favorable for the treatment of COVID-19 caused by SARS-CoV-2 infection.