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May 2015
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1,452 Reads
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9 Citations
Next-generation whole genome sequencing of microbes demands rapid, robust, and scalable library construction workflows, capable of generating high-quality sequence data across a wide range of genome sizes, complexities and genomic GC content. In this Application Note, we describe a streamlined library preparation method that results in minimal bias, high uniform coverage, and facilitates de novo assembly of microbial genomes.
... The total RNA was extracted and sequenced as described above. The total DNA was extracted and sequenced as described elsewhere 10,48,[58][59][60][61][62] . The Illumina HiSeq 2000 with 100 paired-end chemistry was used for MFMB-03 and MFMB-08. ...
May 2015
