Bo Lv’s research while affiliated with Beijing Institute of Technology and other places

... pathway 23 . Since the acteoside biosynthesis shares a common upstream metabolic pathway with many other natural products, its upstream steps have been extensively studied and are nearly elucidated 24 . In the downstream pathway involving acyltransferase and glycosyltransferase catalytic, several enzyme genes associated with acteoside biosynthesis, such as HCT and UGT, have been identified through transcriptome analysis 25,26 . ...

Reference:

Chromosome-level genome assembly of an important ethnic medicinal plant Callicarpa nudiflora

... modifications, the introduction of hydroxyl functional groups through hydroxylation serves as a prerequisite for glycosylation and methylation of certain aromatic compounds and plays a crucial role in their pharmacological activities [9,10]. Numerous studies have highlighted the exceptional bioactivities of hydroxylated aromatic compounds. ...

Reference:

Exploring the role of flavin-dependent monooxygenases in the biosynthesis of aromatic compounds

... A total of 51 fungal endophytes were isolated from four PhGproducing plants: Echinacea purpurea, Rehmannia glutinosa, Ligustrum lucidum, and Cistanche deserticola. The endophyte Simplicillium sinense EFF1, derived from Echinacea purpurea, demonstrated the capacity to de-rhamnose isoacteoside, resulting in the production of calceorioside B, a multifunctional PhG derivative [45]. Li et al. investigated the relationship between endophytic diversity and the metabolites produced across different tissues of Panax quinquefolius [46]. ...

Reference:

Diversity and Correlation Analysis of Endophytes and Top Metabolites in Phlomoides rotata Roots from High-Altitude Habitats

... Then, heterologous terpenoid synthases catalyze the conversion of GPP, FPP, GGPP, 2,3-epoxy-squalene into diverse terpenoids. Various microorganisms have been successfully employed in the production of terpenoids, including Cyanobacteria [33], Escherichia coli [34], Saccharomyces cerevisiae [35], Kluyveromyces lactis [36], and Bacillus subtilis [37]. Among these, S. cerevisiae serves as model organisms for eukaryotic cells, with a fully sequenced genome and a wealth of genetic tools available for manipulation. ...

Reference:

Heterologous Biosynthesis of Terpenoids in Saccharomyces cerevisiae

... 14 It is a common host for synthesizing various plant-derived triterpenoids including βamyrin, 15 ginsenosides 16 and oleanolic acid. 17 S. cerevisiae possesses a well-established pathway for the synthesis of 2,3-oxidosqualene, a key precursor for cucurbitadienol production. 18 Typically, overexpression of ERG genes (ERGs) in the pre squalene pathway effectively increases the supply of precursors. ...

Reference:

Overproduction of Cucurbitadienol through Modular Metabolic Engineering and Fermentation Optimization in Saccharomyces cerevisiae

... The efficiency and yield of microbial fermentation for nutraceutical production can be significantly improved through process optimization. Critical parameters such as pH, temperature, oxygen levels, and nutrient supply must be precisely controlled to ensure the optimal growth of microorganisms and the production of desired metabolites [158]. Modern fermentation processes now integrate advanced monitoring and control systems, such as Process Analytical Technology (PAT), which provides real-time monitoring of key fermentation parameters, allowing for dynamic adjustments to optimize the fermentation process [159]. ...

Reference:

Where Biology Meets Engineering: Scaling Up Microbial Nutraceuticals to Bridge Nutrition, Therapeutics, and Global Impact

... Compartmentalization, a characteristic feature of eukaryotic cells, establishes microenvironments with distinct pH, ionic strength, or environmental polarity that promote optimal conditions for cellular biochemical reactions. [18] The membranebound organelles are formed with specific functions and biological processes. [19] The membrane-bound compartments such as nucleus, mitochondria, lysosome, vacuole, and peroxisome are formed with a boundary of phospholipid layers ( Figure 2A). ...

Reference:

Front Cover: Artificial Compartments Encapsulating Enzymatic Reactions: Towards the Construction of Artificial Organelles (ChemPlusChem 2/2025)

... Metabolic pathways interact with each other, forming a complex metabolic network. Many studies have sought to elucidate regulation mechanisms and enhance production using advanced omics-based technologies [12][13][14][15][16]. ...

Reference:

Addition of vegetable oil to enhance the biosynthesis of butenyl-spinosyn in a high production strain Saccharopolyspora pogona

... The extensive use of organic solvents and reagents in chemical synthesis exacerbates environmental challenges, including issues with waste disposal due to hazardous substances and safety risks. Given the growing emphasis on green and sustainable development, there is a great interest to develop more environmentally friendly and economically viable production methods [12]. The adoption of eco-friendly and effective synthetic biology techniques for synthesizing and increasing the production of isoflavones can enhance their utilization in various fields, including medicine [11]. ...

Reference:

Advances in synthesizing plant-derived isoflavones and their precursors with multiple pharmacological activities using engineered yeasts

... These cells are classified into different types according to their functions and morphologies, such as type I (dark cells), type II (light cells), type III (intermediate cells), and type IV (basal cells) [33][34][35]. Taste receptors on cell membranes are a class of large transmembrane proteins, including the transmembrane heptahelical domain, the Venus Flytrap module (the ligand-binding locus), and the carboxy-terminal tail [36,37]. When these receptors interact with taste substances, they trigger an intracellular signaling cascade, causing the cell to release neurotransmitters. ...

Reference:

Research Progress of Taste Biosensors in Simulating Taste Transduction Mechanism