Birger Lindberg Møller's research while affiliated with IT University of Copenhagen and other places

Publications (35)

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Acacia sensu lato (Fabaceae: Mimosoideae) was recently retypified and divided into five genera worldwide: Acacia, Acaciella, Mariosousa, Senegalia and Vachellia. Acacia sensu stricto is now considered to be a large genus of predominantly Australian origin comprising seven sections: Acacia, Alatae, Botrycephalae, Juliflorae, Lycopodiifoliae, Plurine...
Article
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Mass spectrometry based imaging is a powerful tool to investigate the spatial distribution of a broad range of metabolites across a variety of sample types. The recent developments in instrumentation and computing capabilities have increased the mass range, sensitivity and resolution and rendered sample preparation the limiting step for further imp...
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Chemical defences are key components in insect–plant interactions, as insects continuously learn to overcome plant defence systems by, e.g., detoxification, excretion or sequestration. Cyanogenic glucosides are natural products widespread in the plant kingdom, and also known to be present in arthropods. They are stabilised by a glucoside linkage, w...
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Cytochrome P450 oxidoreductase (POR) is the primary electron donor in eukaryotic cytochrome P450 (CYP) containing systems. A wealth of ensemble biophysical studies of Cytochrome P450 oxidoreductase (POR) has reported a binary model of the conformational equilibrium directing its catalytic efficiency and biomolecular recognition. In this study, full...
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Cyanogenic glucosides are nitrogen‐containing specialized metabolites that provide chemical defense against herbivores and pathogens via release of toxic hydrogen cyanide. It has been suggested that cyanogenic glucosides are also a store of nitrogen that can be remobilized for general metabolism via a previously unknown pathway. Here we reveal a re...
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Localisation of metabolites in sorghum coleoptiles using Raman hyperspectral imaging analysis was compared in wild type plants and mutants that lack cyanogenic glucosides. This novel method allows high spatial resolution in situ localization by detecting functional groups associated with cyanogenic glucosides using vibrational spectroscopy. Raman h...
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Cyanogenic glucosides are present in many plants, including eudicots, monocots, and ferns and function as defence compounds based on their ability to release hydrogen cyanide. In this study, the diurnal rhythm of cyanogenic glucoside content and of transcripts and enzymes involved in their biosynthesis was monitored in cassava plants grown in a gla...
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Gene-editing technology, such as CRISPR/Cas9, holds great promise for the advancement of science and many useful applications technology. This foundational technology enables modification of the genetic structure of any living organisms with unprecedented precision. Yet, in order to enhance its potential for societal benefit, it is necessary to ada...
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Vitex agnus-castus L. (Lamiaceae) is a medicinal plant historically used throughout the Mediterranean region to treat menstrual cycle disorders and is still used today as a clinically effective treatment for premenstrual syndrome. The pharmaceutical activity of the plant extract is linked to its ability to lower prolactin levels. This feature has b...
Chapter
Vanillin is the world’s most popular flavour compound. It is the key constituent of the natural vanilla flavour obtained from cured vanilla pods. The isolation of vanillin from vanilla pods is a laborious and costly process. Currently, less than 1% of the globally produced vanillin is derived from vanilla pods, while the greater part is produced sy...
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Carminic acid, a glucosylated anthraquinone found in scale insects like Dactylopius coccus, has since ancient times been used as a red colorant in various applications. Here we show that a membrane-bound C-glucosyltransferase, isolated from D. coccus and designated DcUGT2, catalyzes the glucosylation of flavokermesic acid and kermesic acid into the...
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Oximes (R1R2C = NOH) are nitrogen containing chemical constituents which are formed in species representing all kingdoms of life. In plants, oximes are positioned at important metabolic bifurcation points between general and specialized metabolism. The majority of plant oximes are amino acid-derived metabolites, formed by the action of a cytochrome...
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Almond bitterness is the most important trait for breeding programs since bitter-kernelled seedlings are usually discarded. Amygdalin and its precursor prunasin are degraded by specific enzymes called β-glucosidases. In order to better understand the genetic control of almond bitterness, some studies have shown differences in the location of prunas...
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Diterpenoids are high value compounds characterized by high structural complexity. They constitute the largest class of specialized metabolites produced by plants. Diterpenoids are flexible molecules able to engage in specific binding to drug targets like receptors and transporters. In this review we provide an account on how the complex pathways f...
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Vanillin is the most important flavour compound in the vanilla pod. VpVAN catalyzes the conversion of ferulic acid and ferulic acid glucoside into vanillin and vanillin glucoside, respectively. Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI) of vanilla (Vanilla planifolia) pod sections demonstrate that vanillin glucoside is...
Article
The cover feature shows the partial assembly of a carmine puzzle. Co-expression of genes from Aloe arborescens (top left), Streptomyces (bottom left) and Dactylopius coccus (bottom right) in Nicotiana benthamiana (background) lead to an accumulation of dcII, a glucosylated octaketide anthraquinone and a precursor of the popular natural colorant car...
Article
Carminic acid is a C-glucosylated octaketide anthraquinone and the main constituent of the natural dye carmine (E120) possessing unique coloring, stability and solubility properties. Despite being used since ancient times, longstanding efforts to elucidate its route of biosynthesis have been unsuccessful. Herein is reported a novel combination of e...
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Low molecular weight compounds are typically used by insects and plants for defence against predators. They are often stored as inactive β-glucosides and kept separate from activating β-glucosidases. When the two components are mixed, the β-glucosides are hydrolysed releasing toxic aglucones. Cyanogenic plants contain cyanogenic glucosides and rele...
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Background The glucosyltransferase UGT76G1 from Stevia rebaudiana is a chameleon enzyme in the targeted biosynthesis of the next-generation premium stevia sweeteners, rebaudioside D (Reb D) and rebaudioside M (Reb M). These steviol glucosides carry five and six glucose units, respectively, and have low sweetness thresholds, high maximum sweet inten...

Citations

... In fact, the recent refining of the Acacia s.l. classification and the review of their phytochemistry indicate that Senegalia genus likely harbor less toxic compounds compared to their relatives, namely Acacia s.s., Vachellia, and Faidherbia (Jaeger et al., 2019;Seigler, 2003). It could be asserted from this inventory that Senegalia feathery shoots and seeds are promising sources of food for humans. ...
... Ice can also be used for embedding but the delocalisation of water-soluble chemicals can be an issue. OCT is not recommended for MSI methods due to the interference with analytes and ion suppression [45]. The preparation of hydrated tissue samples is often done by cryo-sectioning, wherein the samples are flash-frozen and sectioned at around −20 • C. For dry samples such as those obtained from certain plant stems, sectioning can also be done at room temperature conditions [46]. ...
... Prunus, Locus, and Sedum), de novo biosynthesized, or both. 11,80 Cyanogens derive from the amino acids Val, Ile, Leu, Phe, and Tyr, and the non-proteinogenic amino acid cyclopentenyl glycine. 19 In arthropods, the ability to synthesize CNglcs showed a scattered phylogenetic distribution, indicating that it probably evolved independently several times. ...
... In addition, alkaloid biosynthetic pathways usually involve enzymes from the large cytochrome P450 superfamily that catalyze a wide range of chemical modifications. Eukaryotic cytochrome P450s are typically membrane-bound enzymes located at the endoplasmic reticulum that depend on colocalized cytochrome P450 reductases for electron transfer from NADPH and whose activity is modulated by the membrane and cytosolic environment [11][12][13] . These features often hinder the production of functional plant cytochrome P450s in conventional microbial hosts and as a consequence, only about 200 functionally distinct plant cytochrome P450 genes have been identified to date, of which about one tenth are involved in alkaloid biosynthesis 14 . ...
... Gene-editing technology, such as CRISPR-Cas9, holds great promise for the progression of science and applied technologies. This foundational technology enables the modification of the genetic structure of any living organism with unprecedented precision [136]. The recent development and scope of the CRISPR-Cas system have raised new regulatory challenges worldwide due to moral, ethical, safety, and technical concerns associated with its applications in pre-clinical and clinical research, biomedicine, and agriculture [137]. ...
... Also, the supplementation of nitrogen fertilizers to mature plants enhanced CYP79A1 and CYP71E1 action, ultimately leading to higher dhurrin content. Glutathione-S-Transferase (GST) are the predominant enzymes in the metabolic plasticity scheme allowing Sorghum to overturn the resource flow between specialized and general metabolism of growing tissues (Bjarnholt et al., 2018). Dhurrin content can be regulated at the transcriptional level by modulating the activity of biosynthetic and catabolic enzymes; thus, extensive understanding of the metabolism of dhurrin would be helpful in enhancing the yield and pest resilience of plants . ...
... PPD is characterized by initial blue/black discoloration and organoleptic changes in the storage roots within 24-72 hr of harvest, which severely renders the storage roots unpalatable and unmarketable [6]. Furthermore, cassava usage is impeded by potentially toxic quantities of cyanogenic glycosides, linamarin, and lotaustralin in all plant organs with exception of the seeds [7] and this demands the need for cassava storage roots to be processed before eating to remove or reduce their levels. ...
... Genomic co-localisation of dhurrin biosynthetic genes and its transporter gene are likely to protect from self-toxicity of chemical defence (Darbani et al., 2016). However, dhurrin localisation in vacuoles could not be corrobated by Raman microspectroscopy, which demonstrated dhurrin localisation within the cytoplasm and apoplast, in molar concentrations (Heraud et al., 2018). This lack of certainty regarding the locations of dhurrin in Sorghum plants needs to be addressed, which would benefit genetic engineering to produce dhurrin modulated transgenic plants. ...
... Sample preparation for MSI does not require whole-tissue homogenate, furthermore, within-tissue spatial distribution profiles of metabolites in plants can be acquired through MSI (Bong et al., 2016). Recently, MSI has been employed to visualize certain target molecules in various plant organs, such as Hypericum perfortum roots (Tocci et al., 2018), Ginkgo biloba leaves (Li et al., 2018), Vitex agnus-castus fruits/leaves (Heskes et al., 2018) and strawberry fruits (Enomoto et al., 2018). Meanwhile, it has been used to visualize the spatial distribution of some important medicinal compositions in medicinal plants Catharanthus roseusm (Dutkiewicz et al., 2021) and Camellia sinensis (Liao et al., 2019). ...
... Birger Lindberg Møller (ORCID: 0000-0002-3252-3119), Oliver Gericke (ORCID: 0000-0002-8638-6797), Allison Maree Heskes (ORCID: 0000-0002-2732-5185), Nikolaj Lervad Hansen (ORCID: 0000-0002-5938-16591), Gustav Blichfeldt Pedersen (ORCID: 0000-0002-7830-718X), Plant Biochemistry Laboratory, University of Copenhagen: Birger Lindberg Møller is a plant biochemist specializing in the biosynthesis and function of plant natural products, metabolomics, metabolic engineering and environmentally benign production of natural products using heterologous hosts (Luo et al., 2017;Knudsen et al., 2018). A special focus has been on pathways in which the coveted steps are catalyzed by membrane bound cytochrome P450 enzymes (Knudsen et al., 2018;Jensen et al., 2021). ...