Bing Huang's research while affiliated with Guangdong Pharmaceutical University and other places

Publications (6)

Article
Full-text available
Camptotheca acuminata Decne (Nyssaceae) is a major natural source of the anticancer drug camptothecin (CPT) and its derivatives. The leaves and stems are two of the main regions that accumulate CPT in C. acuminata. In this study, we successfully isolated cambial meristematic cells (CMCs) and dedifferentiated cells (DDCs) from C. acuminata stems and...
Article
Full-text available
Angelica sinensis (Oliv.) Diels is an important traditional Chinese medicine, and the medicinal position is its root. This perennial herb grows vigorously only in specific areas and the environment. Tissue culture induction of callus and plant regeneration is an important and effective way to obtain large scale cultures of A. sinensis. The objectiv...
Article
Licochalcone A, as main constituent of Chinese Gancao (Glycyrrhiza inflate Bat), is responsible for the chemopreventive effect of the herbal. In order to get the bioactive modified molecules of Licochalcone A, it was biotransformed using transgenic crown galls of Panax quinquefolium to afford 9 products including a new alkaloid together with 8 know...
Article
Polygonum multiflorum is an important medicinal plant. Hairy roots systems obtained by transforming plant tissues with the natural genetic engineer Agrobacterium rhizogenes can produce valuable biological active substances, which have immense potential in the pharmaceutical industry. To optimize the inductive and cultural conditions of P. multiflor...
Article
The crude polysaccharides were extracted from fruits of Psidium guajava Linn. by hot water. After removal of proteins, isolation and purification by DEAE-52 Cellulose chromatography and Sephadex G-75 gel filtration, a polysaccharide (GP70-2) was obtained and structurally characterized. GP70-2 has a relative molecular weight of 74 kDa and was compos...
Article
To optimize the culture medium of transgenic Polygonium multiflorum hairy root and the effect of different elicitors on its growth. Measured the growth curve of hairy root in four media, including MS, 1/2 MS, B5 and White and estimated the effects of elicitors on the hairy root biomass. MS medium was the most suitable of the four media for hairy ro...

Citations

... Camptothecin showed broad-spectrum anti-tumor activity by inhibiting topoisomerase 1, an enzyme responsible for unwinding DNA during replica-tion [202]. However, camptothecin was highly toxic and less potent in showing anticancer activity compared to its natural derivative, 10-hydroxy camptothecin [203]. The anticancer potential of 10-hydroxy camptothecin was further enhanced through conjugation using gold nanoparticles [204]. ...
... Callus relative growth rate (CRGR) was evaluated by plotting natural logarithm of callus growth versus time with the equation below (Huang et al., 2015): ...
... Some of the molecules have high bioavailability after being ingested and exert biological effects [13]. The effective compounds are further categorized into eight subtypes as shown in Fig. 1a, flavones (compounds [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20], flavonols (compounds [21][22][23][24][25], isoflavones (compounds [26][27][28][29][30], flavanones (compounds [31][32][33][34][35], chalcones (compounds [36][37][38][39][40][41][42][43][44][45], anthocyanidins (compounds [46][47], and flavanonols (compounds [48][49][50][51][52][53][54][55][56], xanthones (compounds 57 and 58) [13,[16][17][18][19][20][21][22][23][24][25][26][27][28][31][32][33][34][35][36][37][38][39][40][41]. ...
... Meanwhile, the hairy roots growth kinetics and nutrient consumption findings revealed that they had a sigmoidal curve and that the best inoculation period for them was 18-21 days. The analysis of the anthraquinone component shown that the hairy roots had a rhein concentration of 2.495 g/g, which was 2.55 times greater than that of natural plants [91]. ...
... The molecular weights of the SLPs were determined using a published method with some modifications (Hua et al., 2014). The Mw of the SLPs was calculated by high-performance gelpermeation chromatography (HPGPC) with 0.02 M monopotassium phosphate as eluent at a flow rate of 0.5 mL/min. ...