Ben Adlam's research while affiliated with Harvard University and other places

Publications (4)

Preprint
We study a general setting of neutral evolution in which the population is of finite, constant size and can have spatial structure. Mutation leads to different genetic types ("traits"), which can be discrete or continuous. Under minimal assumptions, we show that the marginal trait distributions of the evolutionary process, which specify the probabi...
Article
Full-text available
Recent reports have suggested that self-propagating CRISPR-based gene drive systems are unlikely to efficiently invade wild populations due to drive-resistant alleles that prevent cutting. Here we develop mathematical models based on existing empirical data to explicitly test this assumption for population alteration drives. Our models show that al...
Preprint
Full-text available
Recent reports have suggested that CRISPR-based gene drives are unlikely to invade wild populations due to drive-resistant alleles that prevent cutting. Here we develop mathematical models based on existing empirical data to explicitly test this assumption. We show that although resistance prevents drive systems from spreading to fixation in large...

Citations

... These efforts include rational target-site design, regulation of the expression and timing of the nuclease, multiplexing drive targets, improved regulatory elements, and targeting ultra-conserved regions in essential genes that are recorded in the drive, enabling the selective elimination of nonfunctional NHEJ-repaired alleles (21,52,60,(66)(67)(68). While these future drive designs are expected to be more efficient than current systems, it should be noted that even imperfect drives with modest homing efficiencies are still predicted to be quite invasive (69), and will likely be useful for field implementation which could confer long-lasting entomological and epidemiological impacts. ...
... If the introduction frequency is lower than the threshold, it's expected that the drive allele will be lost from the population. Zero-threshold drives such as CRISPR homing drives and Y-linked Xshredders are fast, powerful drives that can be considered "global" drive systems due to their ability to invade from small starting populations [16][17][18][19][20][21] (though high fitness costs can sometimes move these into more confined categories). An intermediate category consists of "regional" drives that lack an introduction threshold when they have ideal performance but gain one if there are any imperfections in the drive that lead to even a small fitness cost (and occasionally other types of imperfections for some drive types). ...