Barbara Mickowska's research while affiliated with University of Agriculture in Krakow and other places
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Publications (64)
The chemical composition, antioxidant properties, and sensory aspects of sponge cakes with the addition of flours from edible insects (buffalo worm, cricket, and mealworm) were evaluated. The addition of edible-insect flours increased the protein, fat, and dietary fiber content in all cases. The utilization of edible insects demonstrated a notable...
Brussels sprouts provide bioactive compounds with widely acknowledged health-promoting effects observed in various levels: single cells, organs and tissues, or the whole organism. However, the choice of the appropriate hydrothermal processing is critical to sustain the nutritional values and cytoprotective activities, as Brussels sprouts are rarely...
Six types of nut-based bars with the addition of edible insect flour were obtained. Flours made from three different insects (Tenebrio molitor L., Acheta domesticus L., Alphitobius diaperinus P.) were used at two different additive levels (15% and 30%) in relation to the weight of the nuts. The addition of insect flour significantly increased prote...
Solid-state fermentation (SSF) is widely recognised as a technique to increase the bioactive potential and nutritional value of plant materials. However, the effect of this biotreatment differs for individual substrates. This study aimed to evaluate the impact of SSF with filamentous fungi (Rhizopus, Aspergillus, and Neurospora) on a moringa leaf p...
The effect of replacement of wheat flour with buckwheat flour at levels of 10, 20, 30, 40, and 50% on nutritional, texture, and physicochemical characteristics of bread was studied. Among others, parameters such as amino acid profile, antioxidant properties, and inositol phosphate content were determined. Amino acid score was calculated in order to...
Effect of insect flour on bread supplementation was evaluated. Such parameters like chemical composition of flours including amino acids composition, fatty acid profile, nutritional value, dough rheology, texture, and color were determined. The protein content of the insect flours ranged from 49.89% to 62.51%, fat from 8.37% to 29.64%, and total di...
Wheat germ cake (WGC) is a nutritionally valuable by-product of oil production from wheat germ, of broad but not yet fully used potential for food applications. Solid-state fermentation with Rhizopus oligosporus and R. oryzae was tested as a new method of WGC bioprocessing aimed to obtain compact products overgrown with mycelium, characterised by a...
Most cells spend the majority of their life in the non-proliferating, quiescent state. Transition to this state is crucial for microorganisms to survive long starvation periods and restart divisions afterwards. Experimental evolution allowed us to identify several mutation in genes that are presumably important for such transition in yeast cells. M...
Abstract Yarrowia lipolytica is an oleaginous yeast species with the ability to grow on a number of substrates types, especially industrial wastes. This paper concerns the statistical optimization of fermentation parameters and media to ensure consistent and improved Y. lipolytica protein production. A strain of Y. lipolytica A-101 was observed to...
Increasing demand for food production requires improvements as well as openness to new sources of protein. Single Cell Protein derived from microbes has been intensively studied as a supplement for traditional sources of proteins, both for animal feed and direct human consumption. Food grade yeast, including Saccharomyces cerevisiae, has already be...
Spelt wheat (Triticum aestivum) is an eco-friendly low-input crop whose consumption is considered advantageous. In this paper, a procedure for obtaining tempe-type products from whole grains of spelt and green spelt (immature grains) is presented, and the effect of biotreatment on the bioactive and nutritional parameters is described. The soluble a...
Solid-state fermentation of plant materials with food grade moulds is considered a method of obtaining bioactive food products and additives. However, the use of quinoa seeds as a substrate in such a process has not been thoroughly studied to date. In this paper, extended fermentation with Rhizopus oligosporus, Aspergillus oryzae and Neurospora int...
The aim of this work was to characterise the biological and sensory profile of biscuits enriched with green (1 and 3%) and black tea (1 and 3%) powders. Biscuits without the addition of tea were used as a control. Phenolic concentration, flavonoid concentration, and antioxidant activity were determined spectrophotometrically. Amino acid composition...
Celem pracy było porównanie składu chemicznego i wartości wypiekowej mąki typu 500 z pszenicy zwyczajnej z mąką z pszenicy orkiszowej uzyskanych podczas przemiału laboratoryjnego ziarna uprawianego w gospodarstwach ekologicznych. Materiałem doświadczalnym były mąki i chleby pszenne wypieczone z tych mąk. Oceniono wartość technologiczną mąki, w tym...
Single Cell Protein (SCP) can be obtained from various microorganisms by growing them on a number of types of substrates, including industrial products waste. This work focuses on non-conventional yeast Yarrowia lipolytica, and its application in the process of production of valuable components like SCP and amino acids. It also analyses the possibi...
The intestinal epithelial cells reside in close proximity to myofibroblasts and microbiota, which are supposed to have an impact on intestinal stem cells fate and to influence processes of tissue maturation and regeneration. Mechanism underlying these phenomena and the diversity among vertebrates can be studied in 3D organoid cultures. We investiga...
Three freshwater fishes: common carp, rainbow trout and northern pike were chosen for the evaluation of amino acid and fatty acid composition—fresh and after smoking. Smoking led to the increase in almost all amino acids, with the highest amount of EAA in common carp. This process led also to the enhancement in the level of SFA and the decrease in...
In this research, coloured quinoa seeds were subjected to a modified oncom-type processing (pre-cooking followed by solid- state fermentation with Neurospora intermedia) to obtain convenient food products of improved parameters. As compared to raw material, the products contained a higher level of protein (on average by 44 %, of the advantageous es...
The effect of 40 h solid-state fermentation with Rhizopus oligosporus on selected parameters of white and coloured quinoa was studied, as compared to standard (30 h) product and cooked seeds. The reducing power (RP) and the activity against synthetic free radicals of standard tempe were higher by on average 140% (white) and 64% (coloured quinoa) th...
Background. The aim of this study was to compare the biochemical and immunochemical properties of avenins in some special oat raw materials and additionally the possibility of using them as a raw material for the gluten-free bakery products.
Materials and methods. The compared oat raw materials were – oat flakes, commercial oat flours (includ- ing g...
Celiac disease is one of the most prevalent food intolerances worldwide, with a gluten-free diet as the only effective therapy. Therefore, searching for new alternative approaches is a priority, and one of them is the idea of using proteinases for degradation of gluten proteins. The aim of the study was a trial of enzymatic cleavage of prolamins ex...
The identification and quantification of cereal storage proteins is of interest of many researchers. Their structural or functional properties are usually affected by the way how they are extracted. The efficiency of extraction process depends on the cereal source and working conditions. Here, we described various commonly used extraction protocols...
The research material consisted of grains of six genotypes of oats (Avena sativa). The grains of three of them had a brown glume (‘Gniady’, ‘CHD 2875', ‘CHD 2833') and the grains of other three genotypes had a yellow one (‘Bohun’, ‘Deresz’, ‘Cwał'). The impact was assessed of their genotype, interactions, orthogonal contrast (oat with yellow glume:...
The effect of fermentation with Rhizopus oligosporus, roasting and cooking on functional parameters and nutritional composition of unroasted buckwheat groats was studied. Tempe showed 57 and 124% higher activity against 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical cation (ABTS(filled circle)+) than cooked unroasted and roasted gro...
Gliadins represent alcohol-soluble fraction of wheat storage proteins which is responsible for development of celiac disease. The only and effective treatment for celiac disease is strict adherence to a gluten-free diet excluding any food made with wheat, as well as rye, barley and possibly oat flour. Enzymatic modification of wheat gliadins seems...
The aim of this study was to reduce the formation of acrylamide during roasting of chicory roots by soaking the fresh roots in a solution
of calcium chloride, by the use of different temperature and time of roasting of dried roots, as well as by the addition of the enzyme
(asparaginase) during roasting of dried roots.
It was shown, that with inc...
The increased interest in rational nutrition causes, that from many years is observed a growing consumption of bread, and novel food supplemented with health promoting components. For the bread production in Poland mainly wheat and rye cake flours are used, depleted of a many valuable nutrients such as protein, dietary fibre, minerals and vitamins....
Kvass based on traditional technology completely disappeared from polish market. It was replaced by drinks, prepared from malt concentrates, wrongly named kvass. The aim of this study was therefore to obtain traditional bread kvass (by fermentation), using the mash prepared from commercial wholemeal rye bread, produced by 5-phase dough fermentation...
The study analyzed the influence of the degree of apple pectin methylation and the concentration of xanthan gum on physicochemical properties of fresh wet egg white protein based foams. Molecular characterization of applied hydrocolloids was performed and amino acid composition of proteins was established. First, essential physicochemical propertie...
Commercial oat flour and certified gluten-free wholemeal oat flour were used to make muffins according to a recipe for gluten-free bakery wares. The use of oat flours instead of a mix of rice and maize flours with maize starch caused the content of the following components in the muffins produced to significantly (p ≤ 0.05) increase: total proteins...
Tempeh is a traditional Indonesian food of high nutritional quality, obtained due to fungal fermentation of dehulled, soaked and cooked legumes. The aim of the research was to study the effect of Lactobacillus plantarum DSM 20174 activity on selected parameters of tempeh made from unhulled seeds of common bean (Phaseolus vulgaris). L. plantarum cel...
Cold-pressed flaxseed oil-cake is rich in nutrients and bioactive compounds. The aim of the study was to examine to what extent the inclusion of this by-product as a fermentation co-substrate can influence the nutritional value of tempeh obtained from grass pea seeds. Flaxseed oil-cake introduction to grass pea seeds (5 - 35% w/w) resulted both in...
The nutritional value of the protein of carp from breeding technologies currently employed in Poland (semi-extensive, low-intensive and high-intensive ones) was evaluated. The total protein content was from 16.9% to 18.6% and did not diverge from the content of this nutrient in other fish species. The protein of the studied carps was characterised...
The aim of this study was to define the influence of microbiological activity on the safety (microflora composition, biogenic amine amount) and colour of "kumpia wieprzowa" during the 3-month ripening period. The study included the amount of aerobic bacteria, yeast, lactobacilli rods, coagulase-negative cocci, pH and colour parameters as well as th...
The aim of this study was to determine the content of protein, fat, and carbohydrates in
selected homogenised cheeses, available on Polish market. Results were compared with the
values declared by the manufacturers. The main protein content was equal to 6.6 g/100 g, fat
content – 4.7 g/100 g, and carbohydrates content – 11.3 g/100 g. However, conte...
The aim of this study was to assess the importance of validation and uncertainty estimation related to the results of amino acid analysis using the ion-exchange chromatography with post-column derivatization technique. The method was validated and the components of standard uncertainty were identified and quantified to recognize the major contribut...
2013): Nutritional value of the protein of consumer carp Cyprinus carpio L. Czech J. Food Sci., 31: 313–317. The nutritional value of the protein of carp from breeding technologies currently employed in Poland (semi-extensive, low-intensive and high-intensive ones) was evaluated. The total protein content was from 16.9% to 18.6% and did not diverge...
The nutritional value of the protein of carp from breeding technologies currently employed in Poland (semi-extensive,
low-intensive and high-intensive ones) was evaluated. The total protein content was from 16.9% to 18.6% and did not
diverge from the content of this nutrient in other fish species. The protein of the studied carps was characterised...
Prolamins are alcohol-soluble fraction of cereal proteins involved in immunological response
of patients with celiac disease. The aim of this study was to analyse the similar protein
complex of selected varieties of cereal, pseudocereal and legume grains by comparison of protein
fractions, amino acids composition and SDS-PAGE electrophoresis. The i...
It is widely accepted that oxidized low-density lipoproteins and local infections or endotoxins in circulation contribute to chronic inflammatory process at all stages of atherosclerosis. The hallmark cells of atherosclerotic lesions—monocytes and macrophages—are able to detect and integrate complex signals derived from lipoproteins and pathogens,...
Tempeh is a popular Indonesian product obtained from legume seeds by solid-state fermentation with Rhizopus sp. The aim of this research was to study the effect of simultaneous mixed-culture fermentation of grass pea seeds on selected parameters of products as compared to traditional tempeh. The inoculum contained different ratios of Rhizopus oligo...
The aim of this study was to compare the prolamin complex of several varieties of
cereals: 16 varieties of wheat (including common, durum and spelt wheat), 8 varieties of
barley, 3 varieties of triticale and 1 variety of rye. In amino acids composition the major part
represent glutamic acid in all type of prolamins (38 – 43 %) but there were some d...
The aim of this study was to investigate possibilities and advisability of the use of oats insoluble protein preparation for the production of wheat bread, in order to increase the amount of protein and biological value of protein in this kind of bakery. Research material consisted of the preparation of insoluble oats protein, wheat flour and wheat...
Jerusalem artichoke (Helianthus tuberosus L.) is grown primarily for its edible tubers, which were first cultivated by native Americans before the arrival of the Europeans. Unlike most tubers, but in common with other members of the Asteraceae, the tubers store fructans instead of starch. Fructans are non-digestible carbohydrates considered functio...
Background: High nutritional value of residual oat flour, which is a by-product in the production β-D-glucan concentration BETAVEN, was the reason to make a trial to apply it in the production of wheat and wheat-rye bread. The aim of the study was to establish a formulation for wheat and wheat-rye bread, in which part of wheat flour would be replac...
The aim of the work was to determine the effects of the freezing and canning processes, followed by a 12-month storage, on the amino acid content of Pleurotus ostreatus mushrooms. The pre-treatment involved blanching, or soaking and blanching, in mushrooms in water or in solutions containing sodium metabisulphite, citric acid, l-ascorbic acid and/o...
Celiac disease is an inflammatory condition of the small intestine in genetically susceptible individuals caused by ingestion of wheat gluten and corresponding proteins from barley and rye. Cereal storage proteins (prolamins) are responsible for immunological response of patients with celiac disease. Prolamins are alcohol soluble fractions, namely...
The CCZ1 gene is a member of the class B VPS (vacuolar protein sorting) genes and it is engaged in the last stage of delivery of multiple kinds of cargo to the yeast vacuole. In the process of fusion of the multivesicular body (MVB) with the vacuole, Ccz1p forms a complex with Ypt7p. Both genes are non-essential for vegetative growth, but their del...
A high nutritional value of oat flour constituting a by-product derived from manufacturing a BETAVEN β-D-glucan concentrate suggested that the oat flour could be used as an addition in wheat and wheat-rye breads. The objective of the research project was to develop a recipe of wheat and mixed breads, where a portion of wheat flour was substituted w...
The aim of this research was to study the antioxidant and nutritional (selected objects) properties of protein isolates obtained from grass pea seedlings as compared with soaked and raw seeds. Two percent extract of isolate from 5-day-old seedlings showed the highest total antioxidant activity (25%) and the ability to chelate Fe²+ (2.35 mg/g d.m.)...
The main serine proteinase inhibitors of rainbow trout (Oncorhynchuss mykiss) and common carp (Cyprinus carpio) blood plasma were isolated and purified. The investigated inhibitors, α1-proteinase inhibitor (α1-PI) and antithrombin III (AT III), act by forming stable complexes with target proteinases. The association rate constants k
on for the inte...
Gluten-free confectionery products were used as controls for comparison with the products, which included different supplements such as linseed meal, amaranth and/or buckwheat. The latter were expected to increase nutritional values of confectionery products. Cookies were analyzed in terms of volume, selected textural parameters (hardness, cohesive...
Nutritional parameters of grass pea tempeh in comparison with products obtained with the addition of sunflower seeds were evaluated. Mixed tempeh was characterised by significantly increased amount of lipids with higher polyunsaturated fatty acids (PUFA) (60%) content and improved n-6 to n-3 fatty acids ratio (16:1). The addition of sunflower seeds...
The highly efficient Capillary Electrophoresis (CE) is a method used for the separation, identification, and quantitative analysis of many compounds contained in food products. Owing to the diversity of techniques offered by CE, this method can be used to determine contents of both the high-molecular compounds, such as proteins or DNA fragments, an...
Neutrophil elastase (NE) and cathepsin G (CG), the proteolytic enzymes localized in azurophil granules of neutrophils (PMN), are involved in PMN responses to various stimuli. When released at sites of inflammation, they participate in the degradation of numerous proteins involved in the regulation of the immune response. In this study, we employed...
The horse apomyoglobin 56-131 peptide is a convenient object for studies on the recently discovered antimicrobial activities of haem-binding protein fragments called haemocidins. The purpose of this study was to determine the effect of this peptide on planar lipid bilayer membranes and on liposomes of different lipid compositions. Micromolar concen...
A fast, isotope-free method for the determination of parameters for the interactions of proteins with thiamine and related compounds was developed. The free and bound forms of a ligand (thiamine or a fluorogenic analogue) were separated by ultrafiltration using commercially available centrifugal protein microconcentrators (Nanosep, Pall Filtron). T...
Citations
... As already mentioned, edible insects are a valuable alternative source not only of vitamins, minerals, proteins, fats, and calories, but also bioactive compounds from the polyphenol group, as proven in just a few publications in the last few years [26][27][28]. Regarding the analyzed nut bars with the addition of flours from edible insects, the chemical composition (including protein content, amino acid composition, fatty acid profile, etc.) was described in an earlier publication [15]. The abovementioned data are available in Supplementary Materials (Tables S1-S3). ...
... The amino acid composition was determined following the method described by Starzyńska-Janiszewska et al. [18]. The samples were placed in a 6 mol/L hydrochloric acid solution containing 0.5% phenol and incubated at 110 • C in an oven for 24 h. ...
... Similar effects on color-parameter values were observed by Pauter et al. [34], who used cricket flour in muffin production. On the other hand, Kowalski et al. [6] observed an increase in the levels of red and yellow color components and total color change in sponge cakes containing mealworms. In that case, chicken eggs were substituted with a plant-based replacement, which may have contributed to the different content of individual color components [6]. ...
... Por otra parte, los valores de metionina se encuentran dentro de lo reportado por Diana et al. (2014), quienes examinaron el perfil de aminoácidos libres, en once panes comerciales y doce panes artesanales con y sin masa madre de Barcelona (España), los ingredientes fueron harina integral, harina de trigo blanco y harina de soya tostada, encontrando que los panes sin masa madre tenían menos contenido de aminoácidos (de 0,03 de metionina hasta 8,11 de ácido aspártico g/100 g) que los panes con masa madre (de 0,12 de metionina hasta 10,89 de ácido aspártico g/100 g). De manera similar Kowalski et al. (2022) encontraron aminoácidos esenciales (0,1672 a 0,7351 g/100 g) y aminoácidos no esenciales (0,1948 a 3,8660 g/100 g) en panes con trigo Cerraceno. Coţovanu et al. (2023) indicaron la presencia de solo seis aminoácidos esenciales (isoleucina, leucina, metionina, fenilalanina, treonina y valina) en pan de trigo, al adicionar 7,89; 9,39 y 9,41% de harina de kiwicha a la mezcla de pan. ...
... Cappelli et al. [11] and Khuenpet et al. [58] found that high levels of cricket or mealworm powder negatively affected the specific volume of wheat bread. On the other hand, Kowalski et al. [22] observed that the addition of mealworm powder flour improved the volume of wheat bread. It should be noted that the observed differences in the specific volume of the enriched bread with mealworm powder flour may be related to the different proportions of insect flour used for the preparation of the breads. ...
... When the applied electric field overcomes the surface tension of the solution, a charged jet is ejected toward the collector forming fine highly charged droplets commonly shaped in spherical morphologies (Castro Coelho et al., 2021;Librán et al., 2017;Moreno et al., 2022). WG, a main by-product of the wheat milling industry, provides concentrated nutrients of biological components such as essential amino acids, unsaturated fatty acids, minerals, vitamins (B and E), dietary fiber, and phytosterols (Jamdar et al., 2021;Liaqat et al., 2021;Mohammadi et al., 2021;Starzyńska-Janiszewska et al., 2021;Zhao et al., 2021), thus can be a potential candidate for prebiotics (Boukid et al., 2018). Live probiotics along with prebiotics have been suggested with innumerable health beneficial potentials (Rashidinejad et al., 2020). ...
... The Q/NQ population balance can be affected by many factors (22). It has been shown that the Q/NQ ratio can be modified by selection to some extent; however, both Q and NQ cells appear to always be present in stationary populations (14,20,24). This raises the important question of how the Q/NQ balance evolves in various ecological scenarios. ...
... The natural processes of yeast cells, such as complexation, extracellular precipitation, transformation, intracellular compartmentalization, efflux systems, crystallization, adsorption onto cell walls, and pigments, allow them to rapidly adapt to metal-contaminated environments, tolerate and detoxify them [36][37][38][39]. Additionally, yeasts have the ability to employ a range of renewable carbon sources, and their biomass has been utilized to produce single cell oil and single cell protein [40][41][42]. ...
... A hidrólise enzimática de polímeros em alimentos é um processo utilizado para melhorar propriedades físicas, químicas e funcionais dos alimentos, mantendo o valor nutritivo e facilitando a absorção das proteínas (Pacheco et al., 2005;Cândido et al., 2003), elevando o valor nutricional com propriedades funcionais adequadas a sua aplicação, além de obtenção de peptídeos com atividades biológicas específicas (Roslan et al., 2014b;Yarnpakdee et al., 2015;Vázquez et al., 2017;Giannetto et al., 2020). Hidrolisado proteico é um produto resultante da ação controlada de enzimas proteases sobre alimentos proteicos, regulando temperatura e o pH durante a reação enzimática para obter produto com peso molecular apropriado (Tkaczewska et al., 2020;Amiza et al., 2017;Roslan et al., 2015;Cândido 1998), sendo que a concentração de enzimas pode afetar a eficiência da reação enzimática (Finkler et al., 2022). A produção de hidrolisados proteicos pode incluir uma ampla variedade de produtos, sendo sua produção realizada a partir da hidrólise por meios químicos ou biológicos, sendo a hidrólise química usualmente a mais aplicada na prática industrial, no entanto os processos biológicos utilizando adição de enzimas propiciam produtos com maior valor nutritivo (Martins et al., 2009). ...
... In fermented rice bran, coumaric acid, sinapic acid, and ferulic acid elevated by 20, 24, and 63 folds, respectively, when compared to native rice bran [35]. Ferulic acid increased by 3.82-fold in fermented wholegrain wheat [38]. Some new phenolics were also produced in many plants under SSF such as apigenin and kaempferol that appeared in fermented chia seeds [15], caffeic acid in fermented rice bran [35], apigenin in fermented garden cress seeds [18], and rutin and chlorogenic in fermented chickpeas [39]. ...