B. Haslett's research while affiliated with Durham University and other places

Publications (4)

Article
Full-text available
The N-terminal amino acid sequence of the small subunit of ribulose-1,5=disphosphate carboxylase from the amphidiploidNicotiana tabacum contains two polymorphisms. From examination of the equivalent sequences in the putative parent speciesNicotiana sylvestris andtomentosiformis it is concluded that the amphidiploidNicotiana tabacum has inherited tw...
Article
Summary The N-terminal sequence of the small subunit of Fraction I protein isolated from tobacco was investigated, using an automatic protein sequencer. The amino acid sequence of the first 21 residues is presented.

Citations

... A statistical analysis of the amino acid compositions of the two subunits from a number of species indicated more variation for the small than the large subunit (38). Comparison of the primary structure of the 25 N-terminal amino acids in 6 species has revealed allelic polymorphisms in 13 positions (28,30,42). Four additional amino acids at the C-terminal end characterise the small subunit of the barley enzyme, when compared to that of spinach and tobacco (28,42). ...
... Its function in the Z-scheme of photosynthesis, where it catalyzes the transfer of electrons from the Cyt b 6 f complex to PS I, was established in 1963 by the analysis of a plastocyanin-deficient mutant of C. reinhardtii (Gorman and Levine, 1965). Plastocyanin was biochemically characterized from many land plants, and it was the first Cu protein to have its structure solved (Boulter et al., 1977;Guss and Freeman, 1983). When researchers analyzed algae and cyanobacteria on the other hand, they were unable to isolate plastocyanin from some of them (Wildner and Hauska, 1974;Kunert and Böger, 1975), which led to the finding that a soluble c-type cytochrome could cover its function in many green algae and cyanobacteria (Bohner et al., 1980a,b;Sandmann and Böger, 1980;Briggs et al., 1990;Zhang et al., 1992). ...
... The amino acid sequences obtained are summarized in Figure 5. All sequences were known for tobacco and allowed the unambiguous identification of single subunits: the PsaH protein of Photosystem I ( Obokata et al. 1993), the small subunit of RubisCO ( Gibbons et al. 1975), the-subunit of the ATP synthase ( Deno et al. 1983), cytochrome f, the FeS protein, cytochrome b 6 and the subunit IV of the b 6 f complex ( Madueno et al. 1992;Shinozaki et al. 1986) and the cytochrome b-559 subunit of Photosystem II ( Carrillo et al. 1986). ...
... Nicotiana tabacum which is likely to be the result of a fusion of Nicotiana sylvestris and Nicotiana tomentosiformis (Strobaek et al. 1976). This type of duplication however, can give rise only to dispersed multi-gene families. ...