Aurawan Kringkasemsee Kettawan’s research while affiliated with Mahidol University and other places

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Publications (7)


Schematic diagram of experimental design
Body weight gain throughout the experiment (A), analysed by one-way ANOVA with Tukey’s post-hoc correction (mean ± SD, n = 18). Disease Activity Index (DAI) scores at different time points (B), average DAI score (C), and total DAI score (D) for each group, analysed by Kruskal-Wallis tests followed by Dunn’s post-hoc correction (mean ± SEM, n = 18). Superscripts indicate significant comparisons: a = PC vs. NC, b = PC vs. LC, c = PC vs. MC, and d = PC vs. HC. Clinical manifestations of gastrointestinal discomfort, including diarrhoea and rectal haemorrhage, along with faecal pellets from AOM/DSS-induced mice (E, F).
Gross mucosal lesions in mouse colons (A), with black arrows and dotted lines indicating areas occupied by elevated lesions on the colon mucosa. Alcian blue-stained colonic mucosa micrographs showing tumour multiplicity at 12.5X magnification (B), where white dotted lines represent the dentate lines (anorectal junction) and yellow dotted lines indicate adenomas and microadenomas on the colon mucosa. Tumour burden analysis (C) and tumour multiplicity (D) are expressed as mean ± SEM, and were analysed using Kruskal-Wallis tests followed by Dunn’s multiple comparisons test (n = 6).
Topographic micrographs of colon mucosa stained with alcian blue and methylene blue at 100X magnification, with a 100 µm scale bar (A). Black dotted lines indicate MDF, characterised by a lack or scarcity of mucus-producing cells, as visualized by reduced mucin staining. Yellow dotted lines represent ACF, characterised by elevated and enlarged crypts compared to adjacent cells, slit-like crypt openings, and an increased pericryptal area, indicated by darker blue-stained crypts. Aberrant crypt counts (B), MDF counts (C), and ACF counts (D) were analysed by Kruskal-Wallis test followed by Dunn’s multiple comparisons test (n = 6), presented as mean ± SEM. ACF were categorized by size: small (E), medium (F), and large (G), with analysis performed using one-way ANOVA followed by Dunnett T3 multiple comparisons test (n = 6), presented as mean ± SD.
Micrographs of mouse colon mucosal cells stained with H&E and corresponding IHC serial sections at 200X magnification, with a 100 µm scale bar (A). Dysplastic tissue shows poor cell differentiation, characterized by reduced goblet cells, distorted crypt architecture, and an increased nucleus-to-cytoplasm (N/C) ratio. β-catenin (CTNNB1) positive cells exhibit both cytoplasmic and nuclear IHC staining, while PCNA and cyclin D1 (CCND1) show only nuclear IHC staining. H-scores for CTNNB1 (B), PCNA (C), and CCND1 (D) immunopositive cells, with a maximum value of 300. Dysplasia grading in mouse colon mucosal cells for each group (E). All data were analysed using One-way ANOVA followed by Dunnett T3 test for multiple comparisons correction, presented as mean ± SEM (n = 6).

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Cold-pressed extraction of perilla seed oil enriched with alpha-linolenic acid mitigates tumour progression and restores gut microbial homeostasis in the AOM/DSS mice model of colitis-associated colorectal cancer
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  • Full-text available

December 2024

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18 Reads

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Peerapa Techaniyom

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Aurawan Kringkasemsee Kettawan

The present investigation explores into the influence of dietary nutrients, particularly alpha-linolenic acid (ALA), a plant-derived omega-3 fatty acid abundant in perilla seed oil (PSO), on the development of colitis-associated colorectal cancer (CRC). The study employs a mouse model to scrutinize the effects of ALA-rich PSO in the context of inflammation-driven CRC. Perilla seeds were subjected to oil extraction, and the nutritional composition of the obtained oil was analysed. Male ICR mice, initiated at four weeks of age, were subjected to diets comprising 5%, 10%, or 20% PSO, 10% fish oil, or 5% soybean oil. All groups, with the exception of the control group (5% soybean oil), underwent induction with azoxymethane (AOM) and dextran sulphate sodium (DSS) to instigate CRC. Disease development, colon samples, preneoplastic lesions, dysplasia, and biomarkers were meticulously evaluated. Furthermore, gut microbiota composition was elucidated through 16S rRNA sequencing. The analysis revealed that PSO contained 61.32% ALA and 783.90 mg/kg tocopherols. Mice subjected to diets comprising 5% soybean or 10% fish oil exhibited higher tumour incidence, burden, multiplicity, and aberrant crypt counts. Remarkably, these parameters were significantly reduced in mice fed a 5% PSO diet. Additionally, 5% PSO-fed mice displayed reduced proliferative and pro-inflammatory markers in colon tissues, coupled with an alleviation of AOM/DSS-induced gut dysbiosis. Notably, PSO demonstrated inhibitory effects on colitis-associated CRC in the AOM/DSS mice model, achieved through the suppression of proliferative and pro-inflammatory protein levels, and mitigation of gut dysbiosis, with discernible efficacy observed at a 5% dietary concentration.

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Antioxidant and Anti-Inflammatory Activities of the Extract and Bioaccessible Fraction of Mango Peel in Muffins

July 2024

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67 Reads

Food Technology and Biotechnology

Research background. Mango peel is a production waste and can cause environmental problems, but its nutritional value consists of bioactive compounds that could be beneficial for human health. The aim of this study is to determine the bioactive compounds, antioxidant and anti-inflammatory activities of mango peels and their use in muffins. Experimental approach. The content of polyphenols, carotenoids and total phenols as well as the antioxidant activity of mango peel extract were evaluated. The anti-inflammatory activity of the extract was investigated using Caco-2 cell assay. The mango peel powder was then incorporated into muffin formulations. The sensory properties of these fortified muffins were evaluated. The total phenolic content, antioxidant activity and anti-inflammatory properties of the muffin extracts and their bioaccessible fractions were also analysed. Results and conclusions. The mango peel contained quercetin, phenolic compounds, α-carotene, β-carotene and lutein, which have antioxidant potential. In Caco-2 cells exposed to induced inflammation, the mango peel powder extract (γ=10, 50 and 100 μg/mL) attenuated the production of reactive oxygen species (ROS), tumour necrosis factor-alpha (TNF-α) and interleukin-8 (IL-8), while maintaining cell viability. Muffins supplemented with 5 % mango peel powder showed good sensory properties, but not as good as those of plain muffins without mango peel powder. The total phenolic content and antioxidant activities in both the extract and the bioaccessible fraction of the supplemented muffins were higher than those observed in the standard muffins. Moreover, the extract and bioaccessible fraction of the supplemented muffins resulted in a higher reduction of ROS, IL-8 and TNF-α production in Caco-2 cells than those obtained from the standard muffins. Novelty and scientific contribution. This study is the first to investigate the protective effects of mango peel and muffins supplemented with mango peel powder against IL-1β-induced oxidative damage in Caco-2 cells. The results confirm that both mango peel and the supplemented muffins inhibited the production of inflammatory markers, including ROS and cytokines. These findings suggest that mango peel could be a valuable component of functional food formulations including dietary supplements.


Cold‐pressed perilla seed oil: Investigating its protective influence on the gut–brain axis in mice with rotenone‐induced Parkinson's disease

June 2024

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105 Reads

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1 Citation

Perilla seed oil, derived from a regional plant native to northern Thailand, undergoes cold‐pressing to analyze its bioactive components, notably alpha‐linolenic acid (ALA). ALA, constituting approximately 61% of the oil, serves as a precursor for therapeutic omega‐3 fatty acids, EPA and DHA, with neurodegenerative disease benefits and anti‐inflammatory responses. This study administered different concentrations of perilla seed oil to male C57BL/6 mice, categorized as low dose (LP 5% w/w), middle dose (MP 10% w/w), and high dose (HP 20% w/w), along with a fish oil (FP 10% w/w) diet. An experimental group received soybean oil (5% w/w). Over 42 days, these diets were administered while inducing Parkinson's disease (PD) with rotenone injections. Mice on a high perilla seed oil dose exhibited decreased Cox‐2 expression in the colon, suppressed Iba‐1 microglia activation, reduced alpha‐synuclein accumulation in the colon and hippocampus, prevented dopaminergic cell death in the substantia nigra, and improved motor and non‐motor symptoms. Mice on a middle dose showed maintenance of diverse gut microbiota, with an increased abundance of short‐chain fatty acid (SCFA)‐producing bacteria (Bifidobacteria, Lactobacillus, and Faecalibacteria). A reduction in bacteria correlated with PD (Turicibacter, Ruminococcus, and Akkermansia) was observed. Results suggest the potential therapeutic efficacy of high perilla seed oil doses in mitigating both intestinal and neurological aspects linked to the gut–brain axis in PD.


Rice Bran Oil Improves Emphysema in Cigarette Smoke Extract-Induced Mice through Anti-Inflammatory and Antioxidative Effects

January 2024

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56 Reads

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3 Citations

Citation: Kettawan, A.; Ruangklai, S.; Rungruang, T.; Thongam, J.; Kettawan, A.K.; Nirmal, N.; Srisuma, S. Rice Bran Oil Improves Emphysema in Cigarette Smoke Extract-Induced Mice through Anti-Inflammatory and Antioxidative Effects. Nutrients 2024, 16, 433. Abstract: Lung inflammation and alveolar enlargement are the major pathological conditions of chronic obstructive pulmonary disease (COPD) patients. Rice bran oil (RBO), a natural anti-inflammatory and antioxidative agent, has been used for therapeutic purposes in several inflam-matory diseases. This study aimed to investigate the anti-inflammatory and antioxidative effect of RBO on a cigarette smoke extract (CSE)-induced emphysema model in mice. The results indicated that CSE significantly induced airspace enlargement in mouse lung. Increased inflammatory cells, macrophage, and TNF-alpha levels in bronchoalveolar lavage fluid (BALF) were noticed in CSE-treated mice. RBO (low and high dose)-supplemented mice showed decreased total BALF inflam-matory cell, macrophage, and neutrophil numbers and TNF-alpha levels (p < 0.05). Additionally, the administration of RBO decreased the mean linear alveolar intercept (MLI) in the CSE-treated group. Additionally, RBO treatment significantly increased the total antioxidant capacity in both mouse BALF and serum. However, RBO did not have an effect on the malondialdehyde (MDA) level. These findings suggested that RBO treatment ameliorates lung inflammation in a CSE-induced emphysema mice model through anti-inflammatory and antioxidant pathways. Therefore, the supplementation of RBO could be a new potential therapeutic to relieve the severity of COPD.


Modification of Physiochemical and Techno-Functional Properties of Stink Bean (Parkia speciosa) by Germination and Hydrothermal Cooking Treatment

December 2023

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115 Reads

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1 Citation

Foods

Stink bean, Parkia speciosa, is recognized as a significantly underutilized legume with versatile utility and diverse benefits. However, information on the impact of different processing methods, such as germination and hydrothermal cooking, is scarce on stink beans (SBs). Therefore, the current research aimed to explore the efficacy of germination (G) and hydrothermal cooking (HTC) on the physiochemical properties, proximate composition, techno-functional properties, and antioxidant potential of SB flour. Furthermore, Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscopy (FESEM) were employed to assess structural and morphological changes. The results revealed that the physiochemical properties of SB were significantly enhanced through processing, with more pronounced improvements observed during germination. Additionally, SBG exhibited a significantly higher protein content and lower fat content compared to SBHTC and stink bean raw (SBR). Moreover, techno-functional properties such as color intensity, least gelation concentration, and pasting properties were significantly improved in SBG compared to SBHTC and SBR. FTIR analysis of SBG and SBHTC indicated structural modifications in the lipid, protein, and carbohydrate molecules. FESEM examination revealed morphological changes in SBG and SBHTC when compared to SBR. Importantly, SBG exhibited higher antioxidant activity and total phenolic content in comparison to SBHTC and SBR. Therefore, processed SB flour can be incorporated and utilized in product development, highlighting its potential as a plant-based protein source for protein-rich breakfast bars and cookies.


Inhibitory Effect of Dietary Defatted Rice Bran in an AOM/DSS-Induced Colitis-Associated Colorectal Cancer Experimental Animal Model

November 2022

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131 Reads

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9 Citations

Foods

Defatted rice bran (DRB) is gaining immense popularity worldwide because of its nutritional and functional aspects. Emerging evidence suggests that DRB is a potential source of dietary fiber and phenolic compounds with numerous purported health benefits. However, less is known about its chemoprotective efficacy. In the present study, we determined and examined the nutrient composition of DRB and its chemopreventive effect on azoxymethane and dextran sulphate sodium (AOM/DSS)-induced colitis-associated colorectal cancer (CRC) in rats. The results showed the presence of several bioactive compounds, such as dietary fiber, phytic acid, and phenolic acids, in DRB. In addition, DRB supplementation reduced the progression of CRC symptoms, such as colonic shortening, disease activity index (DAI), and histopathological changes. Interestingly, a significant decrease was observed in total numbers of aberrant crypt foci (ACFs) and tumors with DRB supplementation. Furthermore, DRB supplementation suppressed the expression of pro-inflammatory cytokines (IL-6) and inflammatory mediators (NF-κB and COX-2) through the inactivation of the NF-κB signaling pathway. The administration of DRB revealed a negative effect on cancer cell proliferation by repressing the expression of nuclear β-catenin, cyclin D1, and c-Myc. These findings suggest that DRB supplementation mitigates chronic inflammation and cancer cell proliferation and delays tumorigenesis in rat AOM/DSS-induced colitis-associated CRC. Therefore, the establishment of DRB as a natural dietary food-derived chemopreventive agent has the potential to have a significant impact on cancer prevention in the global population.


Effect of Hydrothermal Cooking and Germination Treatment on Functional and Physicochemical Properties of Parkia timoriana Bean Flours: An Underexplored Legume Species of Parkia Genera

June 2022

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72 Reads

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13 Citations

Foods

The present study was undertaken to analyze the impact of germination (NBG) and hydrothermal cooking (NBHTC) on the nutritional profile and physicochemical, functional and microstructural properties of Nitta bean (Parkia timoriana) (NBR) seeds. Results demonstrated that the highest crude protein and fat content could be found in NBG and NBHTC, whereas the ash content was significantly higher in NBG. Compared to NBHTC and NBR, NBG has higher emulsion capacity and stability, with values determined to be 58.33 ± 1.67 and 63.89 ± 2.67, respectively. In addition, the highest color intensity was also reported for NBG, followed by NBHTC and NBR. Likewise, NBG showed complete gel formation at a lower concentration (12 g/100 mL) than NBR flour (18 g/100 mL). Furthermore, structural changes in the lipid, protein, and carbohydrate molecules of NBG and NBHTC were evidenced by FTIR studies. Morphological changes were noticed in dif-ferent samples during microscopic observations subjected to germination and hydrothermal treatment. In contrast to NBR and NBHTC, NBG showed the highest total polyphenol content, ORAC antioxidant, and DPPH radical scavenging activity, which demonstrated the potential utilization of Nitta bean flour as a natural plant-based protein source in food security product formulations.

Citations (5)


... This could potentially be achieved through dietary ingredients that promote the growth of beneficial bacteria), or probiotics (live microorganisms that confer health benefits when administered in adequate amounts). Conversely, the positive correlation between Enterobacteriaceae abundance and disease severity suggests that strategies to decrease the abundance of these potentially harmful bacteria may be warranted.This could potentially be achieved through dietary modifications, antibiotics, or other approaches to modulate the gut microbiome.[16][17][18] The mechanisms by which gut dysbiosis maycontribute to the pathogenesis of Parkinson's Disease (PD) are complex and multifaceted, involving a complex interplay of factors and pathways. ...

Reference:

Gut-Brain Axis Dysfunction in Parkinson's Disease: Early Biomarkers and Therapeutic Potential in Jakarta, Indonesia
Cold‐pressed perilla seed oil: Investigating its protective influence on the gut–brain axis in mice with rotenone‐induced Parkinson's disease

... (Benxi, Liaoning Province, China) and were randomly allocated into four groups: control, model, bexarotene, and model + bexarotene (Fig. 1A). The COPD mouse model was generated according to a previous study with some modifications [11,12]. In the model and model + bexarotene groups, the mice were subjected to passive exposure to mainstream CS for 30 min twice daily, with an interval of 6-8 h, for 30 consecutive days. ...

Rice Bran Oil Improves Emphysema in Cigarette Smoke Extract-Induced Mice through Anti-Inflammatory and Antioxidative Effects

... This connection underscored the substantial impact of fermentation on the composition of traditionally fermented pickled ginger and pickled shallot vegetable products. Pickled stink beans and bud rieng had a high protein content, comparable to stink bean flour with higher protein levels and a lower carbohydrate content [48,49]. Both pickled stink beans and bud rieng are used as traditional vegetable materials and have a high lipid content. ...

Modification of Physiochemical and Techno-Functional Properties of Stink Bean (Parkia speciosa) by Germination and Hydrothermal Cooking Treatment

Foods

... Supplementary Table S2 shows that these anti-cancer compounds were concentrated in the rbNPs, which may explain why rbNPs are cytotoxic to cancer cells. Rice bran induces an anti-proliferative effect linked to β-catenin-mediated cell proliferation [62,63]. β-catenin is a protein related to the Wnt signaling pathway, which is known to contribute to cell proliferation and cancer progression [64]. ...

Inhibitory Effect of Dietary Defatted Rice Bran in an AOM/DSS-Induced Colitis-Associated Colorectal Cancer Experimental Animal Model

Foods

... Vibrational analysis using infrared spectroscopy (Figure 4) showed subtle differences between quinoa varieties. In the ungerminated samples, variations were observed in the intensities of the bands associated with functional groups of starch (2927 cm −1 , 2851 cm −1 , 1025 cm −1 , and 855 cm −1 ; -CH-, -CH 2 -, C-OH, and -OH, respectively) and proteins (1651 cm −1 and 1543 cm −1 ), reflecting differences in the biochemical composition and molecular organization of each variety [26,[71][72][73][74]. During germination, a decrease in the intensity of the starch-related bands is observed, suggesting a partial depolymerization caused by enzymatic activity [56]. ...

Effect of Hydrothermal Cooking and Germination Treatment on Functional and Physicochemical Properties of Parkia timoriana Bean Flours: An Underexplored Legume Species of Parkia Genera

Foods