Attila Ványolós's research while affiliated with Semmelweis University and other places

Publications (29)

Article
Full-text available
Pholiols L-S (1–8), eight undescribed triterpenes were isolated from the sporocarps of the mushroom Pholiota populnea. Various chromatographic techniques, such as open column chromatography, flash chromatography, gel filtration, preparative thin layer chromatography, and HPLC, were applied to purify the compounds. The structure elucidation was carr...
Article
Full-text available
Investigation of the chloroform and ethyl acetate extracts of the edible mushroom Pholiota populnea led to the isolation of eight triterpenes, the undescribed natural products pholiols E–K and the known (+)-clavaric acid. HRESIMS and 1D and 2D NMR spectroscopy were employed to determine the structures of the undescribed compounds. The NOESY spectra...
Article
Full-text available
The detailed mycochemical analysis of the n-hexane extract of Pholiota populnea led to the isolation of four new lanostane diesters, named pholiols A-D (1-4), together with an acyclic triterpene, (3S,6E,10E,14E,18E,22S)-2,3,22,23-tetrahydroxy-2,6,10,15,19,23-hexamethyl-6,10,14,18-tetracosatetraene (5), ergosterol (6), and 3β-hydroxyergosta-7,22-die...
Article
Full-text available
Inonotus nidus-pici is a sterile conk which produces macrofungus, a neglected Central-Eastern European relative of the prized Inonotus obliquus, also known as chaga. Investigation of the methanol extract of the poroid fungus I. nidus-pici resulted in the isolation of citropremide (1), 3,4-dihydroxybenzalacetone (2) , lanosterol (3), ergost-6,8,22-t...
Article
Full-text available
Investigation of the methanol extract of the poroid fungus Fuscoporia torulosa resulted in the isolation of a novel triterpene, fuscoporic acid (1), together with inoscavin A and its previously undescribed Z isomer (2 and 3), 3,4-dihydroxy-benzaldehide (4), osmundacetone (5), senexdiolic acid (6), natalic acid (7), and ergosta-7,22-diene-3-one (8)....
Preprint
Investigation of the methanol extract of the poroid fungus Fuscoporia torulosa resulted in the isolation of a novel triterpene, fuscoporic acid (1) together with inoscavin A and its previously undescribed Z isomer (2 and 3), 3,4-dihydroxy-benzaldehide (4), osmundacetone (5), senexdiolic acid (6), natalic acid (7), and ergosta-7,22-diene-3-one (8)....
Article
Full-text available
In the course of our mycochemical studies the extract of Clitocybe nebularis was investigated with the aim to identify its bioactive secondary metabolites. Multistep chromatographic purification of the MeOH extract of C. nebularis resulted in the isolation of two steroids and an organic acid from the CHCl3 and ethyl acetate soluble fractions. The s...
Article
Hypholoma lateritium is an edible macrofungus with a common distribution in Europe, North America, and the Far East. The aim of this study was to investigate the potential anti‐inflammatory effects of H. lateritium extracts and its isolated steroids: fasciculic acid B (1), fasciculol E (2), fasciculol C (3), lanosta‐7,9(11)‐diene‐12β,21α‐epoxy‐2α,3...
Article
Full-text available
The detailed chemical analysis of the methanol extract of Meripilus giganteus (Pers.) P. Karst. led to the isolation of two new cerebrosides, mericeramides A (1) and B (2) together with cerebroside B (3), ergosterol (4), 3β-hydroxyergosta-7,22-diene (5), cerevisterol (6), 3β-hydroxyergosta-6,8(14),22-triene (7), 3β-O-glucopyranosyl-5,8-epidioxyergo...
Article
The chemical analysis of the methanol extract of Porodaedalea chrysoloma (Fr.) Fiasson & Niemelä afforded the isolation of five compounds (1-5). The first two are phenolic derivatives: methyl (E)-3-(4-methoxycarbonylphenoxy)-acrylate (1) is a new natural product, while methyl 3-(4-methoxycarbonylphenoxy)-propionate (2) was isolated from a natural s...
Poster
Full-text available
Cordyceps militaris and its close relative, Cordyceps sinesnis are well-known medicinal mushrooms in the oriental traditional medicine. Mostly due to its atypical, butterfly pulpa-parasitic lifestyle and rarity, it is considered as the key of longevity in China, Japan and Korea. C. sinensis is also native to Hungary. The aim of our work was the myc...
Article
In the current study effects of fungal extracts on the G-protein-activated inwardly rectifying potassium channel (GIRK1/4) were screened using the automated patch-clamp method. 40 organic (n-hexane, chloroform, and 50% methanol) and aqueous extracts were prepared from 10 mushroom species native to Hungary. Among the examined fungal fractions of dif...
Article
Full-text available
Twelve compounds (1–12) were isolated from the methanol extract of brick cap mushroom (Hypholoma lateritium (Schaeff.) P. Kumm.). The structures of the compounds were elucidated using extensive spectroscopic analyses, including NMR and MS measurements. Lanosta-7,9(11)-diene-12β,21α-epoxy-2α,3β,24β,25-tetraol (1) and 8-hydroxy-13-oxo-9E,11E-octa-dec...
Article
Mycochemical examination of a methanol extract of Scleroderma bovista Fr. (Agaricomycetes) led to the isolation of 7 compounds, which were, to our knowledge, identified for the first time in this species. The chemical structures of these compounds were determined through extensive spectroscopic methods (nuclear magnetic resonance and mass spectrome...
Article
Ten compounds have been identified in the methanol extract of cottonwood mushroom (Tricholoma populinum J.E. Lange), 9 of them for the first time in this species. Besides adenosine (8) and nicotinamide (7) the isolated compounds were ergostane type steroids (1-6) and rare sulfinyladenosine constituents (9 and 10). The chemical structures of these c...
Article
Ten representative Central European phellinoid Hymenochaetaceae species (Phellinus sensu lato) were selected to examine their potential pharmacological activity. In this study 40 organic (n-hexane, chloroform, 50% methanol) and aqueous extracts with different polarities were analyzed for their antimicrobial, antioxidant, and xanthine oxidase (XO)−i...
Article
Mycochemical study of the mushroom Gymnopus fusipes led to the discovery of two new cyclopeptides. The two compounds, named as gymnopeptides A and B, are unprecedented highly N-methylated cyclic octadecapeptides. Detailed spectroscopic studies, Marfey's analysis, and a preliminary molecular modeling study suggested that both are natural cyclic β ha...
Article
In the present work, aqueous and organic extracts of 16 Basidiomycetes mushrooms and 1 Ascomycetes mushroom were investigated in vitro for their antiproliferative activity against HeLa (cervix epithelial adenocarcinoma), A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma), and MCF7 (breast epithelial adenocarcinoma) cells, using the 3-(4,5-...
Article
Mushrooms represent a remarkable and yet largely unexplored source of new, biologically active natural products. In this work, we report on the xanthine oxidase (XO) inhibitory activity of 47 wild-growing mushrooms native to Hungary. Aqueous and organic (n-hexane, chloroform, and 50% methanol) extracts of selected mushrooms from different families...
Article
Multidrug resistance (MDR) is a major cause of failure of cancer chemotherapy. Fifty-eight ecdysteroids, herbal analogues of the insect molting hormone and their semisynthetic derivatives, were tested for their activity against L5178 mouse T-cell lymphoma cells (non-MDR) and their subcell line transfected with pHa MDR1/A retrovirus overexpressing t...
Article
Full-text available
Two new and one known ecdysteroids were identified in the methanolic extract of the roots of Serratula wolffii. The new compounds isolated were ponasterone A-22-apioside (1) and 3-epi-shidasterone (3), together with the known 3-epi-22-deoxy-20-hydroxyecdysone (2). The structures of compounds 1-3 were determined by extensive spectroscopic techniques...
Article
Three new compounds (3, 7, and 11) together with eight known phytoecdysteroids (1, 2, 4-6, and 8-10) were isolated from the rhizomes of common polypody, Polypodium vulgare L. The structures of compounds were elucidated by spectroscopic methods including 1D and 2D NMR measurements. The (1)H and (13)C NMR assignments of compounds 1, 6, 9 and 10 are i...
Article
Investigation of the ecdysteroid constituents of the herb Ajuga reptans var. reptans resulted in the isolation of three new ecdysteroids, named reptanslactone A (2), reptanslactone B (3), and sendreisterone (5), and the known 24-dehydroprecyasterone (1) and breviflorasterone (4). The structures of compounds 1-5 were determined by spectroscopic meth...
Article
The plant kingdom has remained an inexhaustible reservoir of compounds with a potential of further therapeutic use. Ecdysteroids are such biologically active compounds which were discovered in insects, and later their presence was confirmed in many plant species. They have a tremendous potential in the most modern therapy (gene-switch systems). Ecd...

Citations

... Further mycochemical investigations led to the isolation of pholiols E-K and (+)-clavaric acid, some of the triterpenes exerted moderate COX-2 and 5-LOX inhibitory activities. Pholiol F (IC 50 194.5 µM against 5-LOX, and 439.8 µM against COX-2) was found to be the most effective among the investigated compounds [12]. ...
... The p-glycoprotein (ABCB1) efflux pump inhibitory activity of the compounds was also tested against resistant Colo 320 cells and inhibitory activity was found for pholiols A and B and the squalene derivative. In addition, the drug interactions of triterpenes with doxorubicin were also studied by the checkerboard method on Colo 320 cells, and it was observed that pholiols B and D interacted in a synergistic manner, while the acyclic triterpene acted in a very strong synergistic manner [11]. Further mycochemical investigations led to the isolation of pholiols E-K and (+)-clavaric acid, some of the triterpenes exerted moderate COX-2 and 5-LOX inhibitory activities. ...
... A phenolic derivative, osmundacetone 123, and a triterpene steroid ergost-6,8,22-trien-3β-ol 124 were identified from the sterile conk mushroom I. nidus-pici Hymenochaetaceae, Basidiomycota colonizing the living Qercus cerris tree trunk in Hungary. Both compounds manifested moderate antibacterial effects against all tested strains (Pseudomonas syringae, Rhodococcus fascians) except B. subtilis and Aliivibrio fischer, respectively (Garádi et al., 2021). ...
... Neuroprotective, antioxidant, antimicrobial, antiproliferative and cytotoxic activities, immunostimulation of human dendritic cells and strong T helper cell type 1 response ( Yazdani et al., 2020 ) Coprinus comatus trehalose, mannitol, fructose, amentoflavone, daidzein, genistein, syringic acid, ferulic acid, esculetin, scopoletin, umbelliferon Immunomodulatory ( Nowakowski et al., 2020 ) Cordyceps spp ciclosporin, cordymin, cordycepin, cordymin (peptide) Anti-inflammatory, anti-angiogenic, anti-cancer, antioxidant, immunosuppressive ( Sánchez, 2017 ) Cyathus africanus neocyathins K-R, neosarcodonin, allocyathin B2, cyathatriol, ...
... Triterpenes of Hypholoma lateritium exerted a significant inhibitory effect on COX-2 and were able to promote the Nrf2 pathway. Fasciculol C, the hexahydroxylated lanostane, synthesized the highest level of Nrf2 (Ványolós et al., 2020). ...
... The oxygen radical antioxidant capacity (ORAC) assay of components of the edible mushroom Meripilus giganteus revealed the highest antioxidant activity (4.94 mmol TE/g) for stellasterol (8) [91]. ...
... The members of Hymenochaetaceae family (Phellinus s. lato) are considered to be an abundant source of diverse bioactive metabolites, and the pharmacological potential of wild species is intensely studied around the world [1][2][3][4]. The morphologically circumscribed Phellinus s. lato represents a phylogenetically polyphyletic assemblage that includes smaller and more natural genera [5]. ...
... It was shown that 60 suppressed the LPS-induced production of NO and PGE2 and decreased the expression of pro-inflammatory cytokines. 50 [198], DPPH free radical-scavenging assay (IC 50 11.38 µM) [222], GIRK channel inhibitory assay (13% inhibition at 10 µM) [224], lipoxygenase inhibitory assay (IC 50 5.46 µM) [218], NO production inhibition assay (IC 50 > 40 µM) [196], (IC 50 > 30 µM) [6], ORAC assay (antioxidant activity 1.94 mmol TE/g) [91], PTP1B inhibitory activity assay (IC 50 7.5 µg/mL) [77], toxicity to Pinus armandi seedlings assay (lethal rate 95% at 30 µg/mL) [197], trap activity assay (reduction to 28.1% from 332% in control cells) [223] 61a ...
... Hypholoma lateritium (= H. sublateritium), già indicato commestibile da Herbich & al. (1966), probabilmente non è tossico, essendovi utilizzo alimentare in diversi Paesi del mondo (Chuluunbaatar & al. 2019). Si presume che le poche segnalazioni di intossicazioni gastrointestinali siano da ricondurre a H. fasciculare. ...
... Ergosterol peroxide (EP) is a common fungal steroid that has been isolated from several medicinal mushrooms such as Ganoderma lucidum, 1 Sarcodon aspratus, 2 Scleroderma bovista, 3 and G. oerstedii, 4 and has shown interesting biological in vitro activities including anti-inflammatory, 5 immunosuppressive, and antitumor. 6,7 The notable cytotoxicity of EP was shown against various cancer types such as ovarian cancer, 8 liver cancer, 9 prostate cancer, 10 and breast cancer. ...