Armin Kramer's research while affiliated with Max Planck Institute for Molecular Genetics and other places
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Publications (4)
Microarray technology plays an increasing role in proteomic research. We give an overview about recent
developments in this technology focusing on molecular interaction studies using protein and antibody microarrays. We
report about technical aspects in the development of protein microarrays and describe different surfaces and detection
modes. Furt...
We have successfully established a novel protein microarray-based kinase assay, which we applied to identify target proteins of the barley protein kinase CK2alpha. As a source of recombinant barley proteins we cloned cDNAs specific for filial tissues of developing barley seeds into an E. coli expression vector. By using robot technology, 21,500 lib...
Protein array technology has emerged as a new tool to enable ordered screening of proteins for expression and molecular interactions in high throughput. Besides classical solid-phase substrates, such as micro-titre plates and membrane filters, protein arrays have recently been devised with chip-sized supports. Several applications on protein chips...
Citations
... Proteins are immobilized on solid surface e.g. N-hydroxyl succinimide (NHS) derivatized slides or epoxy-and aldehyde-derivatized glass attachment through nitrocellulose (Petralia et al., 2017;Kramer et al., 2004), amines (Gerdtsson et al., 2016) or gel-coated slides (Charles et al., 2004) for attachment through absorption/adsorption, diffusion or nickel coated slides for affinity attachment of His-tagged proteins. The function of surface is not only the physical support but also should also demonstrate highest biding and maintain native conformation of proteins. ...
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... Conventional detection methods for microarrays often use fluorescent dyes, which were detected by irradiation from a laser scanner. Detection methods are discussed intensively elsewhere (Espina et al. 2004;Feilner et al. 2004), while in this study, only new approaches should be pointed out to reach much higher sensitivities. With a special method called MIST (multiple spotting technique) (Angenendt et al. 2003a, b), multiple spotting steps are performed successively to one position. ...
... Therefore, protein microarrays were also employed as a tool for phosphoproteomic analysis, as this allows detection of phosphorylation targets as well as molecular interactions using thousands of immobilized probable protein targets [32][33][34][35]. This technique was first employed for A. thaliana proteins [36]. Before designing the high-throughput microarrays with A. thaliana kinases [37], low throughput microarrays were designed to understand the number of microarrays analyzed for one phosphorylation on one microarray [38,39] followed by medium throughput with barley kinases [40]. ...
