Anna Veselá's research while affiliated with Comenius University Bratislava and other places

... Several adverse factors during intrauterine life have been demonstrated to contribute to inadequate foetal development and foetal programming. The most important ones are malnutrition [9,11], oxygen deprivation [12][13][14], placental insufficiency [15], exposure to excess of glucocorticoids [16][17][18], toxic substances (alcohol, tobacco) [19][20][21] and environmental pollutants [22]. The mechanisms underlying an offspring's predisposition to develop hypertension in adulthood have not been completely addressed. ...

... Восстановление АД у крыс Wistar и спонтанно гипертензивных крыс происходит через 2 недели после имплантации устройства [45]. В литературе описаны следующие варианты анестезиологического пособия у крыс: внутримышечное введение кетамина и ксилазина в дозе 100 мг/кг и 10 мг/кг соответственно, цефуроксим 20 мг/кг/день, кетопрофен 5 мг/кг/ день [46]; ингаляционная анестезия изофлюраном с использованием следующих параметров: индукция 4 % изофлюраном в смеси с 100 % кислородом, поддерживающая анестезия -1,5-2,0 % изофлюран в смеси с 100 % кислородом [47]; описано использование 5 % изофлюрана для индукции и 3 % изофлюрана для поддерживающей анестезии (бупренорфин 0,005 мг/кг до 48 часов после операции, триметоприм 30 мг/кг в течение семи дней) [48]; медетомидин + мидазолам + фентанил в количестве 0,15 мг/кг -1 + 2,0 мг/кг -1 + 0,005 мг/кг -1 [49]. Для крупных животных целесообразно использовать комбинированный внутривенный и эндотрахеальный наркоз. ...

... Also similarly to humans, cortisol, neurohormones, renin, and aldosterone cycle in a way to raise HR and blood in the active phase. 4,9,[26][27][28][29] Created with biorender.com. ...

... For instance, Herichova et al. reported that the ACE/ACE2 mRNA ratio showed a clear daily rhythm in the aorta of rats, and that subcutaneous infusion of Ang II modulated the expression of circadian clock genes period circadian regulator 2 (Per2) and neuronal PAS domain protein 2 (Npas2) which diminished the daily rhythm in ACE/ACE2 mRNA ratio. 112 Another study conducted by the same research team also showed significant changes in Per2, Rev-erbα and clock-controlled gene albumin D-box binding protein (Dbp) expression in the heart of rats after Ang II infusion. 113 Nonaka et al. observed a clear circadian rhythm in Per2, Dbp and Bmal1 expression of the mice aorta and showed that treatment with Ang II resulted in a robust upregulation of Per2 gene expression, followed by a marked downregulation that was subsequently followed by synchronous cycling of Per2, Dbp and Bmal1 mRNAs. ...

... The salt resistance in SJL and BALB/c mice may be related to the increase in renal dopamine production and normal D 1 R and D 5 R function with the increase in Na + intake [77][78][79]. In the salt-resistant Wistar rat [80], D 3 R and AT 2 R interact to increase Na + excretion [76]. We propose that a similar mechanism occurs in SR mice normally expressing the wild-type D 2 R. When Na + intake is high, circulating norepinephrine [81][82][83], systemic norepinephrine spillover [84], urinary norepinephrine [85], and renal norepinephrine overflow [86] are decreased, relative to when Na + intake is low, keeping BP in the normal range [87]. ...

... However, HR exhibits circadian fluctuations, and values measured at specific times of the day should reasonably be accepted. The reason for such an approach is the fact that telemetry studies in awake rats indicate the existence of a circadian rhythm in HR, not only in males (Hashimoto et al., 1999(Hashimoto et al., , 2001Koresh et al., 2016;Molcan et al., 2013Molcan et al., , 2014Wessel et al., 2007) but also in females (Koresh et al., 2016;Schlatter & Zbinden, 1982). Therefore, in this regard, the use of the HRV method can be an effective and non-invasive tool for the assessment of autonomic control of the heart, as well as autonomic modulation of HR (Akselrod, 1988;Lunqvist, 1990;Malik & Camm, 1993;Mansier et al., 1996), for which changes are a useful indicator of tendencies F I G U R E 1 Graphical representation of sex differences in individual heart rate variability (HRV) parameters depending on the light-dark (LD) cycle in rats under zoletil anaesthesia. ...

... For GLUT1 and MCT1, the existing data point to the possibility that these transporters are not rhythmically expressed in the BBB [76,166]. However, we need to take into account that circadian rhythms are influenced by factors like sex and species, as well as the fact that GLUT1 and MCT1 show rhythms in other tissues or cell lines, and even the glut1 gene has been shown to be regulated by the neuronal PAS domain protein 2 NAPS2 in a human cell line [167][168][169][170]. These data, together with the point that these receptors and transporters are common targets for nanoformulations, makes the nanocarriers which contain the receptors and transporters addressed here targets with the potential to be tested according to biological rhythm. ...

... However, HR exhibits circadian fluctuations, and values measured at specific times of the day should reasonably be accepted. The reason for such an approach is the fact that telemetry studies in awake rats indicate the existence of a circadian rhythm in HR, not only in males (Hashimoto et al., 1999(Hashimoto et al., , 2001Koresh et al., 2016;Molcan et al., 2013Molcan et al., , 2014Wessel et al., 2007) but also in females (Koresh et al., 2016;Schlatter & Zbinden, 1982). Therefore, in this regard, the use of the HRV method can be an effective and non-invasive tool for the assessment of autonomic control of the heart, as well as autonomic modulation of HR (Akselrod, 1988;Lunqvist, 1990;Malik & Camm, 1993;Mansier et al., 1996), for which changes are a useful indicator of tendencies F I G U R E 1 Graphical representation of sex differences in individual heart rate variability (HRV) parameters depending on the light-dark (LD) cycle in rats under zoletil anaesthesia. ...

... The rhythm of per2 mRNA expression with a peak at the beginning of the dark phase of the LD cycle has been previously described in the PFC of rats (Chun et al., 2015;Herichov a et al., 2017;Otsuka et al., 2020;Soltésov a et al., 2013;Wang et al., 2019). On the other hand, clock and npas2 did not show a rhythmic pattern in the PFC of control animals during the 24 h cycle (Herichov a et al., 2017;Otsuka et al., 2020;Soltésov a et al., 2013;Wang et al., 2019). ...

... The cardiomyocyte clock is also influenced by multiple hormonal factors such as angiotensin II and aldosterone, 2 important therapeutic targets in heart failure. 30,31 The effect of aldosterone on clock gene expression in cultured rat cardiomyoblasts was attenuated but not completely inhibited by spironolactone, an aldosterone receptor inhibitor. 31 Angiotensin II induces the expression of clock genes in vitro, and blocking its receptor abolishes this induction. ...