Anna Szpitter's research while affiliated with University of Gdansk and other places

... Therefore, we can obtain a big amount of homogenous plant material under well defined in vitro conditions. In vitro cultivated plants of this species produce wide range of secondary metabolites, such as flavonoid compounds (glycosides of kaempferol and quercetin), anthocyanins, tannins (derivates of ellagic acid and its glycosides) and naphthoquinones (plumbagin is the major naphthoquinone followed by minor ones such as chloroplumbagin and 8,8 0 -biplumbagin) (Miyoishi et al. 1984;Kreher et al. 1990;Hook 2001;Babula et al. 2006a, c;Krolicka et al. 2006). Synthesis of different secondary metabolites depends on cultivation media composition, most of all, on the content of macro-and microelements. ...

... Therefore, we can obtain a big amount of homogenous plant material under well defined in vitro conditions. In vitro cultivated plants of this species produce wide range of secondary metabolites, such as flavonoid compounds (glycosides of kaempferol and quercetin), anthocyanins, tannins (derivates of ellagic acid and its glycosides) and naphthoquinones (plumbagin is the major naphthoquinone followed by minor ones such as chloroplumbagin and 8,8 0 -biplumbagin) (Miyoishi et al. 1984; Kreher et al. 1990; Hook 2001; Babula et al. 2006a, c; Krolicka et al. 2006). Synthesis of different secondary metabolites depends on cultivation media composition, most of all, on the content of macro-and microelements. ...

... glucosides, flavonoids, phenolic acids) that are used as substrates in the production of pharmaceuticals (Kreher et al., 1990, Finnie andvan Staden, 1993). Our previous studies have shown that chloroform and methanol extracts from Drosera sp. and Dionaea tissues have a great antimicrobial potential (Krolicka et al., 2008(Krolicka et al., , 2009(Krolicka et al., , 2010Szpitter et al., 2014;Taraszkiewicz et al., 2012). This study answers the question whether secondary metabolites from carnivorous plants are a good basis for AgNPs synthesis and whether the resulting AgNPs would possess higher antimicrobial activity than the extracts that were used for their preparation. ...

... According to previous reports, P. aeruginosa cells survived at 400 µ g mL −1 of the naphthoquinone [13], whereas its cytotoxic effect on eukaryotic cell lines is observed at a definitely lower concentration, with an IC50 ranging from ca. 0.6 to ca. 9.6 µ g mL −1 [40]. Our study confirms the weak susceptibility of P. aeruginosa to carnivorous plant extracts, previously reported only for Drosera aliciae [13,41], as well as its intrinsic resistance to a broad range of secondary metabolites, i.e., naphthoquinones, flavonoids and phenolic acids. ...

... Carnivorous plants from Nepentheceae and Droseraceae have been used in folk medicine for a long time in the treatment of various disorders [17,27], and recent investigations have confirmed a wide range of activities of both crude extracts and isolated compounds [20,37,51,86].For example, it has been evidenced that Dr. rotundifolia and Dr. tokaiensis extracts have anti-inflammatory potential [45] and N. bicalcarata shows antimicrobial and antidiabetic activity [87]. Antibacterial activity was also evidenced for N. cultivar 'Miranda' against Klebsiella pneumonia [88], for N. gracilis against Bacillus subtilis and Escherichia coli [89], and for Di. ...

... Elicitation was employed to increase the secondary metabolite content in Drosera and Dionaea cultures, and was performed by the application of biotic and abiotic elicitors. Biotic elicitation of Drosera and Dionaea cultures with Agrobacterium rhizogenes lysates increased PL content by 2.6-fold from 1.1 mg/g fresh weight (FW) in Dionaea muscipula and RAM content by 1.9-fold from 1.2 mg/g FW in Drosera capensis (Krolicka et al., 2008). ...

... alba transformation formed teratomas without hairy root formation. e growth index increased 3.7-fold and the RAM level in the transformed teratomas increased by 1.6-fold to 410 μg/g DW (Krolicka et al., 2010). e biological activity of the NQs derived form in vitro cultured plants have been evaluated. ...

... Similarly, an increase in oil yield and emission of leaf volatiles (up to 21 times greater than control) was observed in Ocimum basilicum exposed to 860-910 MHz and 2.4 GHz (Lung et al. 2016). Changes in secondary metabolite production using EMF-r of different frequencies has also been reported by many other researchers (Orsák et al. 2001;Ye et al. 2004;Królicka et al. 2006;Ramezani et al. 2012). ...