Anna J. Zelaya’s scientific contributions

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Figure 1. Simplified graphic showing the steps of the bean beetle microbiome CURE. Students begin with an introduction on bean beetles, and through guided inquiry generate a research question and hypotheses. Students design and conduct experiments and collect DNA for amplification and sequencing. Once the sequence data are received, they use free web-based tools to analyze their data (in a shaded box to denote that it is the topic being presented here). After analysis is complete, students interpret their data and write a final report, followed by presentations of their findings. For courses that are not conducting a CURE, the process could be simulated via freely downloadable datasets from various sources (S1. Analysis of Microbiomes -Table of next-generation sequence datasets). The lesson presented here will work with a variety of datasets, allowing for flexibility in the curriculum. Students may still go through the steps of the scientific process, such as hypotheses formation, data analysis, and communications of findings.
Analysis of Microbiomes Using Free Web-Based Tools in Online and In-Person Undergraduate Science Courses
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January 2022

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Anna J. Zelaya

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Christopher W. Beck
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