Andréa Haruko Arakaki’s research while affiliated with Federal University of Mato Grosso do Sul and other places

Unconventional approaches can be used control populations of disease-carrying insect to improve health, economic, social, and environmental standards. There is a need for prolonged reduction of vector populations, using low-toxicity and low-cost products in domestic settings. Metals such copper and iron have shown to be toxic to insect larvae, with a delay in reproduction, damage to the cells of the digestive system and the production of free radicals and oxidant species, resulting in tissue damage and death. To control insect breeding, we synthesized and characterized Fe (III) acetate via carbonate. Fe (III) acetate showed no toxic effect on mosquito larvae up to 1.000 mg L⁻¹, but was bactericidal for Gram-positive and Gram-negative bacteria, mainly due to the action of Fe (III) acetate. © 2018, Universidade Federal de Mato Grosso do Sul, Departamento de Quimica. All rights reserved.

Os ativos inseticidas são propostos há décadas a partir de fontes vegetais, toxinas de microorganismos, moléculas sintéticas e, até, utilizando novos complexos metálicos. Este trabalho teve por objetivo a síntese de novos ativos inseticidas que considerem os conceitos da química de coordenação, organização molecular e alterações de rotas bioquímicas no inseto através da atividade de complexos metálicos. Esta síntese foi realizada utilizando complexos baseados em aminopolicarboxilatos (APCs) com metais de transição para atividade abrangente (ovicida, larvicida e biológica), visando à redução populacional do mosquito atingindo suas formas imaturas e impondo restrições na sua cadeia alimentar, pois inseticidas com Cu(II) e Fe(III), inviabilizam os ovos e são tóxicos para larvas de Aedes aegypti (cepa Rockfeller), além de apresentarem intensa atividade biológica para bactérias Gram negativa e Gram positiva e outros microrganismos. Os produtos foram obtidos por duas rotas sintéticas: via sódica e via carbonato. Os resultados mostraram que somente os complexos APCs Na[Cu(NTA)], [Fe(NTA)], Na2[Cu(EDTA)] através dos íons complexos [Cu(NTA)]-1, [Fe(NTA)] e [Cu(EDTA)]-2 possuem atividade inseticida contra as formas imaturas do A. aegypti. Os complexos metálicos de Cu(II) e Fe(III) de MGDA, HEDTA, EDTA e DTPA, apesar de não possuírem atividade inseticida até 1000 ppm, possuem intensa atividade biológica para bactérias Gram positiva e Gram negativa.

The objective of this work was the synthesis of Cu(II) acetate route carbonate for analysis of biological activity in larvae 3rd instar of Aedes aegypti (Rockefeller strain) and bacteries gram-negative and gram-positive. The characterization the sample commercial and synthesized via carbonate was accomplished by FTIR and indicated the formation of Cu(II) acetate by the bands 1425-1625 cm-1. Analyses by UV-Vis showed bands at 244 nm attributed to acetate and 704 nm to the Cu(II). The melting point for commercial sample and route carbonate was 117 ºC. The results of the bioassays for toxicity of Cu(II) acetate CL10 showed 14.48; CL50 CL90 22.81 and 50.00 mg L-1. The antibiograms showed bactericidal activity for grams and concentration-dependent in the range of 10-1 to 10-4 mol L-1. The values of the inhibition zones for bacteria found were 16 to 10 mm of Cu(II) acetate. Results demonstrated significant biological activity for of Cu(II) acetate and the need for continuing studies for investigation of Cu(II) acetate as an alternative to the comprehensive control of immature in breeding, micro / food chain and even the attractiveness of insects females for breeding

The objective of this work was to study the production of biomass with copper bioaccumulation in submerged fermentation using sugarcane molasses. Candida pelliculosa BARU 05 isolated from Baru (Dipteryx alata) was selected for its good capacity to accumulate the copper. Fermentation was carried out using the medium composed by sugarcane molasses at 5 °Brix enriched with (g/L) CuSO4.5H2O 0.1; yeast extract, 10.0; (NH4)2SO4, 5.0 ; KH2PO4, 5.0 MgSO4, 0.5, inoculum 10 % of total volume (100 ml), pH 6.0, and incubation at 30 °C, 120 rpm for 120 h. After three steps of optimization an uptake of 95.04% and 13.397 g/L biomass were obtained. The kinetics of copper bioaccumulation and biomass production was followed in a 10- liter bioreactor in a batch and fed-batch fermentation which showed copper accumulation of 91.98 and 100 %, respectively, and biomass production of 38.85 g/L (24 h) and 57.54 g/L (48 h), respectively.

This work aimed to evaluate the toxicity of some insecticides compounds on Aedes aegypti and Artemia salina larvae. Bioassays were carried out to evaluate the toxic effect after of 24 and 72 h using the compounds or associations. The LC10, LC50 and LC90 values were obtained and utilized for toxicity comparations. For Ae. aegypti, LC50 were 32.65 mg L-1 in 24 h for Na2[EDTA-Cu(II)] and total mortality in 72 h for SAP-Na2[EDTA-Cu(II)].

Aedes aegypti é inseto de grande interesse na Saúde Pública por transmitir o vírus da Dengue, Febre Amarela e Febre Hemorrágica da Dengue. No controle do mosquito utilizam-se inseticidas organoclorados, organofosforados, carbamatos e piretróides que guiam à seleção de insetos resistentes. A combinação de íons metálicos de transição, EDTA, STI e SAP são propostos como estratégia efetiva e persistente para o controle de larvas de Ae. aegypti através de danos ao sistema digestório por radicais livres. A toxicidade dos compostos foi avaliada para Ae. aegypti e A. salina (organismo não alvo). Os bioensaios foram conduzidos para avaliar o efeito tóxico dos compostos e associações após 24 e 72 horas. As CL10, CL50 e CL90 foram obtidas para comparações de toxicidade. Para Ae. aegypti a CL50 foi 32,65 mg L-1 em 24 horas para Na2[EDTA-Cu(II)] e mortalidade total a 62,5 mg.L-1para quelato em polímero acrílico de liberação lenta SAP-Na2[EDTA-Cu(II)] em até 72 horas.

The research for new techniques of in vitro cultiva tion is being object of many studies around the wor ld, in order to optimize and decrease production costs of seedlings with agronomical interest. The main goal of this work was to compare different systems of in vitro cultivations using Ananas comosus L. Merril. So, the in vitro gr owth of the plantlets was promoted in two different bioreactors : Bioreactor of Immersion by Bubbles (B.I.B. ® ) and the Reactor of Temporary Immersion (R.I.T.A. ® ) with immersion cycle every 2 hours for 15 minutes and the traditional system in flasks with 200 mL. All cultivation systems used th e MS liquid nutritive solution, supplemented with B AP (1 mgL -1 ), ANA (0.25 mgL -1 ), sucrose (30 gL -1 ) and Tween 20 ® (0.5 µL). The pH was adjusted to 5.8 and sterilize d at 120°C for 15 minutes. The cultures were kept into a growth ro om during 30 days, with controlled temperature of 2 5±2°C, under white cold light (46.8 μmol.m -2 .s -1 ), with photoperiod of 16 hours. The experimental d esign used was randomized, with three treatments, three repetition s and ten plants each stage. Among the evaluated sy stems, the BIB ® presented the best results for the tested variable s, mainly the total number of shoots, being 2.3 e 3 .1 times superior when compared with the system R.I.T.A. ® and the traditional consecutively. So the system o f immersion by

This work is based on the action of communities, with the example Seating Andalúcia in valuing the importance of natural resource management linked to their own desires, seeking to nature conservation and maintenance of biodiversity. Thus, enhance the collection of native fruit in areas of natural occurrence and provide technical assistance is qualified some of the economic alternatives for local people can create.

The objective of this work was to compare different systems on in vitro multiplication of Oncidium leucochilum. The treatments were two types of bioreactors: RITA ® and BIB ® , both with temporary immersion (30 min each 2 h) and the conventional culture in liquid medium. Culture medium was MS supplemented with 1 mg.L -1 BAP, 0.25 mg.L -1 NAA and 5 µL.L -1 Tween ® 20, the pH was adjusted to 5.8. Cultures were kept in a growth room with 25±2°C, under cool white light (35 M.m -2 .s -1 ), with 16 hours of photoperiod. The experimental design was complete randomized with 3 replicates of 20 explants. After 45 days of in vitro culture was observed that explants cultivated in bioreactor BIB ® showed higher shoot number per explant (6.56), higher fresh mass increment (5.7g), larger dry mass (0.59g) and aerial part height (1.87cm) compared to explants cultured at bioreactor

The objective of this work was to compare different systems on in vitro multiplication of Oncidium leucochilum. The treatments were two types of bioreactors: RITA® and BIB®, both with temporary immersion (30 min each 2 h) and the conventional culture in liquid medium. Culture medium was MS supplemented with 1 mg.L-1 BAP, 0.25 mg.L-1 NAA and 5 μL.L-1 Tween® 20, the pH was adjusted to 5.8. Cultures were kept in a growth room with 25±2°C, under cool white light (35 M.m-2.s-1), with 16 hours of photoperiod. The experimental design was complete randomized with 3 replicates of 20 explants. After 45 days of in vitro culture was observed that explants cultivated in bioreactor BIB® showed higher shoot number per explant (6.56), higher fresh mass increment (5.7g), larger dry mass (0.59g) and aerial part height (1.87cm) compared to explants cultured at bioreactor RITA® and conventional culture. The bioreactor BIB® is a viable alternative, efficient and had lowest cost for in vitro multiplication of O. leucochilum.