Alexander S. Shashkov's research while affiliated with N. D. Zelinsky Institute of Organic Chemistry and other places
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Publications (19)
Two closely related Proteus mirabilis smooth strains, Kr1 and Ks20, were isolated from wound and skin samples, respectively, of two infected patients in central Poland. Serological tests, using the rabbit Kr1-specific antiserum, revealed that both strains presented the same O serotype. Their O antigens are unique among the Proteus O serotypes, whic...
A glycopolymer of novel structure was found in the cell wall of plant pathogen Clavibacter phaseoli VKM Ac-2641T (family Microbacteriaceae, class Actinomycetes). The glycopolymer was (1 → 6)-linked β-d-galactofuranan with side branched trisaccharide, α-D-Manp-(1 → 2)-[α-D-Manp-(1 → 3)]-α-D-Ribf-(1→ at O-2 on every second galactofuranose residue. Th...
Lytic bacteriophage have potential for the treatment of otherwise untreatable extensively antibiotic-resistant bacteria. For Acinetobacter baumannii , most phage exhibit specificity for the type of capsular polysaccharide (CPS) produced on the cell surface.
A structurally diverse capsular polysaccharide (CPS) in the outer cell envelope plays an important role in the virulence of the important bacterial pathogen, Acinetobacter baumannii. More than 75 different CPS structures have been determined for the species to date, and many CPSs include isomers of a higher sugar, namely 5,7-diamino-3,5,7,9-tetrade...
The above-ground part of the Salsola passerine was found to contain ~13% (w/w) of polysaccharides extractable with water and aqueous solutions of ammonium oxalate and sodium carbonate. The fractions extracted with aqueous sodium carbonate solutions had the highest yield. The polysaccharides of majority fractions are characterized by similar monosac...
Novel, closely related phages Possum and Horatius infect Pectobacterium versatile, a phytopathogen causing soft rot in potatoes and other essential plants. Their properties and genomic composition define them as N4-like bacteriophages of the genus Cbunavirus, a part of a recently formed family Schitoviridae. It is proposed that the adsorption appar...
The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies. In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth. Epinephrine...
Bacteriophages and phage polysaccharide-degrading enzymes (depolymerases) are garnering attention as possible alternatives to antibiotics. Here, we describe the antimicrobial properties of bacteriophage KpV74 and phage depolymerase Dep_kpv74 specific to the hypervirulent Klebsiella pneumoniae of the K2 capsular type. The depolymerase Dep_kpv74 was...
A halotolerant hydrocarbon-oxidizing bacterium Halomonas titanicae strain TAT1 was isolated from a petroleum reservoir. The O-polysaccharide (O-antigen) was isolated from the lipopolysaccharide of H. titanicae TAT1 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the repeating linear pentasaccharide O-uni...
Two acylated forms of the higher sugar, 5,7-diamino-3,5,7,9-tetradeoxy-l-glycero-l-manno-non-2-ulosonic acid called pseudaminic acid, Pse5Ac7Ac and Pse5Ac7RHb where R indicates (R)-3-hydroxybutanoyl, have been found to occur in many capsular polysaccharide (CPS) types produced by isolates of an important human pathogen, Acinetobacter baumannii. The...
The K98 capsular polysaccharide (CPS) from the Acinetobacter baumannii clinical isolate, REV-1184, was studied by sugar analysis and Smith degradation along with one- and two-dimensional ¹H and ¹³C NMR spectroscopy and high-resolution electrospray ionization mass spectrometry. The CPS was found to consist of linear tetrasaccharide repeats (K-units)...
Acinetobacter baumannii isolate LUH5552 carries the KL89 capsule biosynthesis gene cluster. Capsular polysaccharide (CPS) isolated from LUH5552 was analyzed by sugar analysis, Smith degradation, and one- and two-dimensional ¹H and ¹³C NMR spectroscopy. The K89 CPS structure has not been seen before in A. baumannii CPS structures resolved to date an...
Fucosylated chondroitin sulfates (FCSs) FCS-BA and FCS-HS, as well as fucan sulfates (FSs) FS-BA-AT and FS-HS-AT were isolated from the sea cucumbers Bohadschia argus and Holothuria (Theelothuria) spinifera, respectively. Purification of the polysaccharides was carried out by anion-exchange chromatography on DEAE-Sephacel column. Structural charact...
The O-polysaccharide (OPS) was isolated by mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G2559 and studied by sugar analysis along with 1D and 2D ¹H and ¹³C NMR spectroscopy. The following structure of the branched pentasaccharide repeating unit was established.
α-d-Glcp
1
↓
4
→6)-α-d-Manp-(1 → 2)-α-d-Manp-(1 → 2)-β-d-Manp...
In this study, several different depolymerases encoded in the prophage regions of Acinetobacter baumannii genomes have been bioinformatically predicted and recombinantly produced. The identified depolymerases possessed multi-domain structures and were identical or closely homologous to various proteins encoded in other A. baumannii genomes. This me...
A comprehensive understanding of capsular polysaccharide (CPS) diversity is critical to implementation of phage therapy to treat panresistant Acinetobacter baumannii infections. Predictions from genome sequences can assist identification of the CPS type but can be complicated if genes outside the K locus (CPS biosynthesis gene cluster) are involved...
Citations
... Phages are recognize with host surface receptors by receptor-binding proteins (RBPs) typically located at th end of phage tail fiber [73]. The capsule of A. baumannii is the most common receptor fo phage [79,166,172,173]. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/997561955528708-1614848712949_Q64/Olga-Timoshina.jpg)
... Equal concentrations of SCCP were prepared and measured in the same manner; the same concentration of ascorbic acid served as a positive control [52]. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/356099929722881-1461912247901_Q64/Vasily-Smirnov.jpg)
... It is worth pointing out that the Kl-dep depolymerase formed a halo even on strains on which the vB_KpnP_Klyazma phage showed lysis from without. This is quite significant since the specificity of the depolymerase is more often the same or even lower than the host range of the phage [48][49][50]. However, the specificity of this depolymerase was quite high, because it had no effect on control strains even with capsule types containing linkages between monosugars similar to KL20 (KL19(Gal β(1-3)GlcA) and KL62 (Man α(1-3)Gal)). ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/277784053207055-1443240287604_Q64/Nikolay-Volozhantsev.jpg)
... In previous decades, a novel area of study has started to develop in microbiology-microbial endocrinology [6]. Human humoral factors such as hormones could now be considered regulators of microbial metabolism and behavior [6][7][8][9]. However, too little is known about the mechanisms of such interkingdom interactions. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/605573980647431-1521391496791_Q64/Andrei-Gannesen.jpg)
... The article by Ustyuzhanina et al. concerns the structural characteristics and blood anticoagulated activity of a series of fucose-rich sulfated polysaccharides from two Indo-West Pacific sea cucumbers Bohadschia argus and Holothuria spinifera collected from Vietnamese shallow waters [10], including fucosylated chondroitin sulfates FCS-BA and FCS-HS, along with fucan sulfates FS-BA-AT and FS-HS-AT. Extensive NMR analysis was used for the structural characterization of the polysaccharides. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/272300146688036-1441932822389_Q64/Nikolay-Nifantiev.jpg)
... Removing the capsules in the context of natural environments may alter interactions of the bacteria to various external factors. For example, capsule-degrading enzymes were shown to lift the protection of microbial cells against immunity factors mediating the in vivo therapeutical effect of phage-derived tail spikes which lack any direct bactericidal activity in vitro [31,32]. The enzymes produced by the phage-infected cells in the course of phage therapy (PT) may thus contribute to the elimination of the non-infected pathogen cells by the immune system. ...
![[object Object]](https://i1.rgstatic.net/ii/profile.image/1160386564898821-1653669124724_Q64/Peter-Evseev.jpg)
![[object Object]](https://i1.rgstatic.net/ii/profile.image/272949420752897-1442087621348_Q64/Anastasia-Popova-7.jpg)
... Phages are recognize with host surface receptors by receptor-binding proteins (RBPs) typically located at th end of phage tail fiber [73]. The capsule of A. baumannii is the most common receptor fo phage [79,166,172,173]. ...
