Abha Agnihotri’s research while affiliated with Amity University and other places

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Publications (9)


Genomic and Transcriptomic Approaches for Quality Improvement in Oilseed Brassicas
  • Chapter

May 2017

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47 Reads

Abha Agnihotri

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Manoj Kumar

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Divya Kilam

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Microbial Symbiosis and Bioactive Ingredients of Medicinal Plants

May 2017

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99 Reads

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8 Citations

Divya Kilam

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Abha Agnihotri

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[...]

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Medicinal plants have been used widely for their bioactive ingredients as they are highly potent and have least side effects. This has led to a surge in demand for medicinal plants for producing higher quantity and good quality bioactive compounds. Symbiotic association of microorganisms with plants has been shown to affect the production and quality of active ingredients. However, the effect is not consistent and is seen to vary under different microbial associations. This chapter elucidates the studies on microbial symbiosis with medicinal plants and the effect of this interaction on medicinally important bioactive ingredients. The role of both nutritional and non-nutritional pathways in this interaction has also been discussed.


Development of an efficient high-performance thin layer chromatography method for determination of jasmonic acid in leaf tissue of Stevia rebaudiana (Bertoni) Bertoni

February 2017

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61 Reads

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5 Citations

Natural Product Research

Determination of endogenous levels of jasmonic acid (JA) is essential, as it plays a pivotal role in the physiological processes during a plant’s life cycle. A high performance thin layer chromatography (HPTLC) method was developed for the detection and quantification of JA in leaf extracts of medicinal plant, Stevia rebaudiana (Bertoni) Bertoni. The separation was achieved using the solvents ethyl acetate–benzene (1:1, v/v) as the mobile phase, followed by scanning and quantification at 295 nm. Densitometric analysis of leaf extract resulted in compact spots for JA at Rf = 0.45 ± 0.02. The linear regression analysis showed good relationship with r value. The recovery rate of JA indicated good reproducibility and repeatability of the assay. The statistical analysis proved the reproducibility of the method; therefore, it can be employed for routine quantification of JA in different tissue samples of S. rebaudiana and may also be extrapolated to other biological samples.


Endophytic root fungus Piriformospora indica affects transcription of steviol biosynthesis genes and enhances production of steviol glycosides in Stevia rebaudiana

December 2016

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175 Reads

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19 Citations

Physiological and Molecular Plant Pathology

Stevia rebaudiana (Bertoni) produces low calorie sweeteners, steviol glycosides (SGs) - stevioside and rebaudioside-A. Varying spore concentrations of P. indica were applied to S. rebaudiana grown in vitro and in the greenhouse to examine the effect on plant growth and synthesis of SGs. Symbiotic association of P. indica showed plant growth promotion and higher SGs content. The transcription profiles of eight key genes of the SGs biosynthetic pathway also showed strong upregulation. The results show that the symbiotic effect of P. indica can be utilized to achieve plant growth enhancement in S. rebaudiana along with increased production of SGs.



Chlamydospores of P. indica in root segments of S. rebaudiana
Effect of inoculation with P. indica (Pi) and A. chroococcum (Az) and co-inoculation on (a) stevioside and (b) rebaudioside-A content (mg/g dry weight) in leaf extracts of S. rebaudiana. The same lower case letters within each column are not significantly different at p ≤ 0.05 as determined by one-way ANOVA and Tukey’s range test. Values are mean ± SD of N = 3
Combined effects of Piriformospora indica and Azotobacter chroococcum enhance plant growth, antioxidant potential and steviol glycoside content in Stevia rebaudiana
  • Article
  • Publisher preview available

July 2015

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228 Reads

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46 Citations

The herb Stevia rebaudiana Bertoni is an important sugar substitute due to the presence of steviol glycosides-mainly stevioside and rebaudioside-A. These compounds possess antioxidant activity with industrial importance. The fungus Piriformospora indica has been used to improve medicinally important biomolecules, growth and secondary metabolite content in plants. The soil bacterium Azotobacter chroococcum has also been reported to possess plant growth promoting ability. The present study was undertaken to test the ability of P. indica (Pi) and A. chroococcum (Az), alone and in combination to improve the growth, antioxidant activity and steviol glycoside (SG) content of in vitro S. rebaudiana plantlets. Inoculation with Pi or Az alone showed enhancement in all these characters compared with non-inoculated plantlets. Combined inoculation with Pi and Az further enhanced these traits.

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Citations (5)


... Confocal images provided insights into its penetration into plant roots, resembling the behavior of other plant growth-promoting fungal root endophytes [13][14][15][16] . Similar observations were made for other Talaromyces species, highlighting their potential for promoting plant growth and tolerance to various stresses [40][41][42][43][44][45][46][47][48] . Furthermore, T. albobiverticillius HNB9 exhibited additional plant growth-promoting properties, including IAA production, zinc, phosphorus, and silica solubilization. ...

Reference:

Isolation and characterization of a newly discovered plant growth-promoting endophytic fungal strain from the genus Talaromyces
Microbial Symbiosis and Bioactive Ingredients of Medicinal Plants
  • Citing Chapter
  • May 2017

... It also enhances plant resistance to biotic stresses such as bacterial, fungal, viral and nematode diseases and abiotic stresses like heavy metals, salinity and drought (Deshmukh and Kogel, 2007;Sherameti et al., 2008;Daneshkhah et al., 2013;Lakshmipriya et al., 2016;Li et al., 2017;Varkey et al., 2018). Significant increase in growth and yield of many medicinal plant species was recorded on inoculation with P. indica (Rai et al., 2001;Kilam et al., 2016). Several reports have also shown the impact of P. indica inoculation on increasing the secondary metabolite content in plants (Dolatabadi et al., 2011a(Dolatabadi et al., , 2011bSatheesan et al., 2012;Das et al., 2012;Prasad et al., 2013;Sharma and Agrawal, 2013;Sharma et al., 2014;Kilam et al., 2017). ...

Endophytic fungus Piriformospora indica: secondary metabolites and yield enhancement in medicinal plants
  • Citing Article
  • September 2016

International Journal of ChemTech Research

... Stevia rebaudiana (Bertoni) is a perennial shrub native to South America, belongs to genus stevia, which comprises different phytochemicals with interesting biological activities, family Asteraceae (Reis Simas et al. 2019;Chac on-Morales et al. 2021). Stevia leaves are characterized by the presence of steviol glycosides which are 300 times sweeter than sucrose (Li et al. 2009;Kilam et al. 2017). Stevioside and rebaudiosides are major components of diterpene glycosides present in stevia leaves. ...

Development of an efficient high-performance thin layer chromatography method for determination of jasmonic acid in leaf tissue of Stevia rebaudiana (Bertoni) Bertoni
  • Citing Article
  • February 2017

Natural Product Research

... The results of some researches have proven that inoculation with P. indica by increasing the expression of some genes associated with MEP and MVA biosynthetic pathways such as 1-deoxy-D-xylulose 5-phosphate synthase (DXS), 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate reductase (IDS), 1-Deoxy-D-xylulose5-phosphate reductoisomerase (DXR), increases the medicinal compounds in plants (Schäfer et al., 2009;Nair et al., 2020). Investigation the effect of P. indica inoculation on growth parameters and glycosides of stevia showed that inoculation with the fungus increased growth parameters (plant height, number of leaves, shoot dry weight and root length), the expression of 1-deoxy--D-xylulose 5-phosphate synthase (DXS), Geranylgeranyl diphosphate synthase (GGDPS), Kaurene synthase (KS) and Kaurene oxidase (KO) genes, as well as the amount of glycosides Stevioside and Rebaudioside (Kilam et al., 2017). Plant growth-promoting bacteria, including Pseudomonas, are another group of microorganisms that have the ability to symbiosis with plants and have been used in recent years to moderate the adverse effects of stress (Meena et al., 2017;Rajkumar et al., 2017;Rasouli Sadaghiani et al., 2019). ...

Endophytic root fungus Piriformospora indica affects transcription of steviol biosynthesis genes and enhances production of steviol glycosides in Stevia rebaudiana
  • Citing Article
  • December 2016

Physiological and Molecular Plant Pathology

... All enzymatic and non-enzymatic antioxidants significantly increased in S. rebaudiana Shou-2 compared with control; a positive effect of PGPB on TPCs in rice has also been described by Chamam, et al. 75 . Furthermore, our finding agreed with the results of TPCs, TFCs, and DPPH in S. rebaudiana due to the combined effect of Piriformospora indica and Azotobacter chroococcum 76 . In comparison to untreated Additionally, the increase of antioxidants may be interpreted by two reasons stimulate the plant's inducible defense mechanisms in the same way pathogenic microorganisms do as a result of hydrolytic enzymes until the plant adapts to bacterial colonization 78 . ...

Combined effects of Piriformospora indica and Azotobacter chroococcum enhance plant growth, antioxidant potential and steviol glycoside content in Stevia rebaudiana