A.L. Farr’s scientific contributions

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Publications (22)


Estimation of protein by the Folin-Ciocalteu reaction
  • Article

January 1968

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19 Reads

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13 Citations

O. Lowry

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N. Rosebrough

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A. Farr

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R. Randall



THE QUANTITATIVE HISTOCHEMISTRY OF BRAIN
  • Article
  • Full-text available

March 1954

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17 Reads

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818 Citations

Journal of Biological Chemistry

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Protein Measurement With Folin Fenol Reagent

December 1951

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1,789 Reads

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161,445 Citations

Journal of Biological Chemistry

Since 1922 when Wu proposed the use of the Folin phenol reagent for the measurement of proteins (l), a number of modified analytical pro- cedures ut.ilizing this reagent have been reported for the determination of proteins in serum (2-G), in antigen-antibody precipitates (7-9), and in insulin (10). Although the reagent would seem to be recommended by its great sen- sitivity and the simplicity of procedure possible with its use, it has not found great favor for general biochemical purposes. In the belief that this reagent, nevertheless, has considerable merit for certain application, but that its peculiarities and limitations need to be understood for its fullest exploitation, it has been studied with regard t.o effects of variations in pH, time of reaction, and concentration of react- ants, permissible levels of reagents commonly used in handling proteins, and interfering subst.ances. Procedures are described for measuring pro- tein in solution or after precipitation wit,h acids or other agents, and for the determination of as little as 0.2 y of protein.




Citations (18)


... The total and inorganic phosphorus were determined using a colorimetric method (P-molybdate reaction) [43]. ...

Reference:

Galectin-2 Agglutinates Helicobacter pylori via Lipopolysaccharide Containing H Type I Under Weakly Acidic Conditions
THE QUANTITATIVE HISTOCHEMISTRY OF BRAIN

Journal of Biological Chemistry

... One unit of LOX activity was defined as the amount of enzyme catalyzing the formation of 1µmol of the hydroperoxide per min under standard assay conditions. Protein concentration was determined by the method of Lowry et al., (1951). Bovine serum albumin (BSA) was used as standard. ...

Protein determination with Folin reagent
  • Citing Article
  • January 1951

Journal of Biological Chemistry

... Protein content was measured using bovine serum albumin as the standard [20]: Solution A was prepared by dissolving 0.1 M NaOH and 2% Na2CO3, while Solution B consisted of 0.5% CuSO4·5H2O dissolved in a 1% sodium-potassium tartrate solution. Solution C, a mixture of Solution A and Solution B in a 50:1 ratio, was freshly prepared just before use. ...

Protein measurement with the Folin phenol method
  • Citing Article
  • January 1951

... The IAA (Indole acetic acid) production by isolates were determined via using colorimetric method in presence as well as in absence of tryptophan by using method of Brick et al. 29 Protein produced by bacterial isolates were estimated quantitively by using method of Lowry et al., 30 Values were determined with standard curve made by using concentrations of IAA ranging from 10-100 µg/ml separately for both IAA and Protein. Finally, the IAA production by isolates were estimated in microgram per gram (µg/g) of protein produced by bacterial isolates. ...

Protein measurement with the Fohn phenol reagent
  • Citing Article
  • January 1951

Journal of Biological Chemistry

... Total proteins and total carbohydrates for both T. aes0vum leaves and C. vulgaris microalga were es>mated as reported by Lowry et al. (1951) and Dubois et al. (1959), respec>vely. The lipid content of C. vulgaris has been determined by Bligh and Dyer method, (1959). ...

Protein measurements by Folin-phenol reagent
  • Citing Article
  • January 1951

Journal of Biological Chemistry

... Glutamic oxaloacetic transaminase (GOT) and glutamine pyruvic transaminase (GPT) were measured by the method of [21]. Proteins content was assayed using the Lowry et al. method [22] using bovine serum albumin (BSA) as standard protein, glycogen content and total lipids were measured by [23,24], respectively. ...

Folin-phenol reagent for measurement of leaf proteins
  • Citing Article

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