A. Troncoso's research while affiliated with Universidad de Sevilla and other places
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Publications (53)
Holm oak (Quercus ilex L.), a typical evergreen tree of the Mediterranean area, is very important due to its ecological and economical values. Propagation
of this species is extremely difficult and traditionally carried out only by seed germination. In this work, mature acorns
were germinated in vitro and in peat substrate in aseptic and non-asepti...
Agronomic practices can modify olive fruit and oil quality. However, there is little information on the influence of fertigation, a common practice in most intensive orchards. We studied nutrient distribution in the soil profile following fertigation with different doses of N-P-K fertilizer, and its effect on nutrient concentrations, yield and both...
A comparative embryological study on two Spanish cultivars and one growing in Bulgaria of Olea europaea was carried out. The embryo sac develops according to Allium (bisporic)-type. The critical phases during the development of male and female gametophyte that lead to sterility and abortiveness of the embryo sacs and ovules are reported. The embryo...
Main processes governing the plant-soil interactions in adult olive (Olea europaea L.) trees under fertigation were studied to better understand the response of the trees to this agricultural practice widely
used in new olive orchards. Our final objective was to obtain soundly based scientific evidences for a rational choice of
the fertilizer dose....
Soils from four estuaries of SW Iberian Peninsula, affected by anthropogenic influence (urban, industrial and agricultural activities), were analyzed for the occurrence of a variety of metals and trace elements including Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, P, Pb, S and Zn. The soils presented very high levels of salinity (high...
This work aims to know the effect of the Glomus intraradices fungus substratum inoculation on the survival, development and nutritive status of cassava, grape and olive plants during hardening. Micropropagated plants of cassava, grape and olive were transplanted individually to 2 L pots filled half of them with steamy sterilized soil and the other...
This study reports the effectiveness of an arbuscular mycorrhizal (AM) fungus Glomus intraradices on three clones (SOM-1, 05 and 50) of cassava (Manihot esculenta). Arbuscular mycorrhizal inoculation increased plant resistance to transplant stress from “in vitro” to “ex vitro” conditions and plant biomass (shoot and root) production was greatly enh...
Fertigation is widely used in the new intensive olive orchards, although there is not enough information for a rational management of this practice. Preliminary results of a fertigation experiment initiated in 1999 in a mature olive orchard of the cultivar 'Manzanilla de Sevilla' are presented. Four treatments were established: T1(no fertilizer) an...
Zygotic mature olive embryos from olive plants growing in the World Olive Plant Collection of Cordoba (Spain) and in high salinity marshes, were cultured in vitro on 1/3 MS medium with different NaCl concentrations: 0, 4, 6 and 8 g L-1 (0, 68.4, 102.6 and 136.9 mM respectively). Close to 100% of the embryos cultured in control medium formed normal...
In this work, differential protein expression in olive tissues after salt treatments has been investigated. Olive clones selected in vitro according to their salt tolerance were submitted, also in vitro, to high salt concentrations. After each treatment, the leaves and stems were collected and protein extracts of them subjected to polyacrilamide ge...
The objetive of this work was to study if the addition of nutrients to the irrigation water modified ‘Manzanilla de Sevilla’ olive pulp and pit growth. The experiment was carried out during the 2003 fruit-growth period in an irrigated orchard near Seville, southern Spain. Fruit samples were taken in July and September, at 12 and 21 weeks after full...
For olive genetic improvement work it is very important to obtain a high rate of seed germination and a shortening of the juvenile period of the plants raised. By in vitro embryo culture, a 100% of germination is attained in a few days and the supply of adequate nutritive solutions and photoperiod speeds up seedling development during hardening, th...
This study determined how arbuscular-mycorrhizal (AM) colonization by Glomus intraradices affected plant biomass and salt tolerance (in terms of growth) of three cassava clones (SOM-1, 05, and 50). Survival, root, stem and leaf production, and nutrient accumulation were determined in AM-inoculated and non-inoculated cassava clones under a range of...
The response of mature olive (Olea europaea L.) trees to both regulated deficit irrigation and fertigation was studied for some consecutive years in sub-humid (Italy) and semi-arid conditions (Spain). All cultivars tested in these experiments were preferentially for table olive consumption (pickling and double-aptitude). Data from these long-term s...
Table olive quality was analyzed in adult 'Manzanilla de Sevilla' olive trees subjected to different N-P-K fertigation treatments through five years (1999-2003). A randomized block design with four fertigation treatments was established: irrigated without fertilizer (control), and T200, T400, and T600 treatments, in which each tree was respectively...
Three cassava clones (SOM-1, “05”, and “50”) were cultured in vitro on MS medium plus sucrose (30g L−1) and myo-inositol (100mg L−1) without plant growth regulators and with additions of 0 (control), 0.5, 1, 1.5, 2, 2.5, and 3g L−1 NaCl to test their salt tolerance. The same cassava clones were cultivated in greenhouse conditions on a sandy soil su...
We report the results of a study carried out in a 'Manzanilla de Sevilla' olive orchard near Seville, Spain, where the influence of different fertigation treatments on oil chemical composition was considered. Four treatments were established: control (no fertilizer) and T200, T400, and T600 in which each tree, respectively, received 200, 400, or 60...
Rhododendron ponticum subsp. baeticum is endemic in the southern region of the Iberian Peninsula. The relict populations of this species are vulnerable, due mainly
to difficult conditions for the establishment of seedlings, resulting in a virtual lack of sexual recruitment. In order to
preserve the surviving populations, in vitro culture methods ha...
In vitro chemotherapy using ribavirin or acyclovir was applied to eliminate Grapevine fanleaf virus (GFLV) from Vitis vinifera cv 'Zalema' plant material. Ribavirin at 20 mgl-1 of concentration eliminated GFLV from the 94% of the grapevine in vitro shoots, enabling GFLV virus free plants to be obtained. The ribavirin treatment did not affect plant...
Populations of wild grapevine, Vitis vinifera L. subsp. sylvestris (Gmelin) Hegi, were discovered in S.W. of the Iberian Peninsula over the last years. Location, ecological aspects, sanitary characteristics, including the ELISA test to detect specific virus attack, are described. In vitro propagation and conservation are also considered. The paper...
The percentage of GFLV-affected plants and its geographical distribution, in "Palomino Garrido Fino", "Zalema" and "Pedro Ximénez" grapevine cultivars from respectively "Aljarafe" of Sevilla, "Condado de Huelva" of Huelva and "Montilla- Moriles" of Córdoba were determined. All the plots in "Aljarafe" zone presented some plants with virus (average 1...
In vitro culture methods were used to germinate olive embryos prior to maturation. Fruit, seed and embryo development were established with consecutive sampling from 20 to 100 days after bloom. For that same period, embryo development and germination success were determined by in vitro culture trials using one-third strength MS medium with or witho...
The protein profiles of juvenile and young olive plant tissues have been compared by SDS-PAGE analysis. Differences were found between the juvenile and young leaves extracts corresponding to the expression of 3 bands of 29, 35 and 63 kD which were partial or totally absent in the sucker leaves extract. This suggests that suckers are not a juvenile...
The influence of sucrose or mannitol on in vitro zygotic embryo germination, seedling development and explant propagation of olive tree (Olea europaea L.) was compared. Embryos germinated without sucrose in the medium but for adequate development of the seedlings to yield viable plants, a carbohydrate supply was necessary; both sucrose and mannitol...
Angelica pancicii (Vands.) is an endemic, endangered, protected species in Bulgaria. This situation is mainly due to the excess of harvesting and the difficulties of seed germination and plant propagation. Seeds from mature fruits harvested in different areas of Bulgaria were used as plant material. Later in Seville (Spain) the seeds were subjected...
Comparisons of protein composition between juvenile and adult organs of olive trees were made by SDS-polyacrylamide gel electrophoresis analysis. Plant material included leaves, bark and bud tissues obtained from juvenile and adult organs within the same plant as well as from separate plants of both the same and different genetic origin. The amount...
The effect of inflorescence number and distribution along the shoot on the level of fruit-set was studied using `ON' year olive trees with a high level of floral differentiation. Reduced levels and different inflorescence distribution patterns were created artificially by hand inflorescence thinning. In most cases, removal of up to 50% of the inflo...
The effect of inflorescence number and distribution along the shoot on the level of fruit-set was studied using `ON' year olive trees with a high level of floral differentiation. Reduced levels and different inflorescence distribution patterns were created artificially by hand inflorescence thinning. In most cases, removal of up to 50% of the inflo...
The feasibility and anatomical development of an in vitro olive cleft-graft method were studied. Grafting survival after 60 d in vitro was 85% which then dropped slightly to 67% after hardening. Three days after grafting, callus formation was observed along the cut surfaces of the rootstock and scion, after 6 d the first healing cellular unions wer...
The response of 11 grapevine rootstock varieties to increasing salt concentrations (0, 50, 85, 120, 155 mM NaCl) was studied under in vitro and growth chamber conditions. The effect of salinity on the mortality of explants was compared with that of plantlets grown under growth chamber conditions and with data in literature on rootstock resistance u...
The effect of different N forms on the growth of olive seedlings in the greenhouse was studied. KNO3, NH4NO3 and (NH4)2SO 4 at different concentrations, as well as two types of substrates, sterile sand and a sand-peat mixture (1: 1), were tested. In sterile sand, with N concentrations higher than 5 mM, serious damage was observed in the plants, so...
Germination of intact seeds, seeds without testa and isolated embryos of two subspecies of Juniperus oxycedrus (oxycedrus and macrocarpa) was compared both in vitro and under greenhouse conditions (for intact and seeds without testa). Intact seeds did not germinate in the greenhouse or in vitro. Seeds without testa did not germinate under greenhous...
Survival and development of cassava (Manihot esculenta Crantz) plantlets has been increased by modifying tissue culture protocols and by mycorrhizal inoculation. About 90% of plantlets were successfully rooted in vitro and 75% survived after the acclimatization phase. Inoculation with Glomus deserticola early in the post vitro weaning stage enhance...
Fifteen different tropane alkaloids and derivatives were identified by GC-MS in various plant organs of Atropa baetica. The main root and leaves displayed the largest number of tropane alkaloids, most of which were also present in lateral roots. In contrast, only five of these alkaloids appeared in stem tissue. Quantitative analysis by HPLC showed...
The germination potential of olive seeds without the sclerified endocarp (stoneless seeds) and growth of the seedlings were compared with germination and growth of excised and in vitro cultured embryos of ten olive cultivars. The number of empty stones and the number of stones with two seeds were also determined for each of the cultivars. Isolated...
In vitro propagation of Atropa baetica was established employing axillary buds. Single buds were cultured through a multiple
shoot induction phase, rooting phase, and then followed by acclimatization in soil. For multiple shoot induction, Murashige
and Skoog (MS) medium with 3% sucrose, supplemented with either 0.75 or 1.25 mg l-1 of BAP provided t...
The effect of flower number and distribution on the fruiting behavior of various olive cultivars was studied over a period of 10 years. The number of staminate flowers within each cultivar had no significant effect on fruitset. Pre-bloom removal of up to 50% of the flowers did not affect fruitset. Variation in prebloom flower-removal position resul...
The influence of the source of inorganic nitrogen (KNO3, (NH4)2SO4 and NH4NO3) and its concentration (5, 10, 20 and 30 mM N) on total N incorporation, as well as on N distribution into different fractions (amminiacal, amino, amide and protein) and on free amino acid levels has been determined in grape vine explants cultured in vitro.Increasing conc...
4 paginas, 1 tabla, 1 referencia.-- Proceedings of the I International Symposium on Olive Growing/International Society for Horticultural Science, Fruit Section-Working Group on Olive.-- Sociedad Espanola de Ciencias Horticolas.-- International Society for Horticultural Science.-- Simposio celebrado del 26-29 de septiembre 1989, en Cordoba, Espana.
Growth and mineral composition of grape-vine explants (13.3 EVEX rootstock) cultured on a basal medium with 10.4 mM of NO3−, and respective additions of 5, 10, 15, 20 and 25 mM of NH4NO3 were analysed.
Low N-availability (10.4 mM) induced low shoot formation. Addition of NH4NO3 up to 15 mM increased number, quality and N levels of shoots. Further i...
Influence of soil nitrogen availability on quality components (sucrose, alpha-amino nitrogen, K+ and Na+) of sugarbeet was studied. This study was carried out on a homogeneous soil of southern Spain. We have tested 0, 100, 150, 200 and 250 kg N/ha. The use of increasing quantities of nitrogen fertilizer leads to a large decrease in sucrose concentr...
Citations
... Thus, refs. [41,42] reported a clear improvement of callogenesis in grapevine, strongly related to manganese sulphate reduction. ...
... Chloride concentration in leaves of plant of the three treatments ranged between 0.067 and 0.83%. These concentrations are within the normal range of concentration of Cl in olive leaves (Bartolini et al., 1991). However, the rainfed treatment had lowers concentrations indicating a limitation of Cl absorption due to lack of water. ...
... Lead pencils are produced from Juniper wood. Similarly, Juniper trees provide timber wood for buildings and outdoor structures etc. (Cantos et al., 1998;Mamo et al., 2011). ...
... Six different culture media were used to establish in vitro cultures of shoot tips: MS-0, Murashige and Skoog (1962) basal medium containing no growth regulators; MS-1, the same basal medium supplemented with 2 mg/l 6-benzyladenine (BA); C 2 D as described by Chée et al. (1984), also enriched with 2 mg/l BA; MS*/2, as used by Spiegel-Roy et al. (1985) containing 1.76 mg/l of 3-indolacetic acid (IAA) and 0.346 mg/l of gibberelic acid (GA 3 ), AND*, as proposed by Troncoso et al. (1988) with 0.1 mg/l naphtalenacetic acid (NAA) and 1 mg/l BA added; and, fi nally, C1*, also used by Troncoso et al. (1990), supplemented with 0.5 mg/l BA. To establish in vitro cultures of nodal segments MS-1 medium was used only. ...
... Aşılamadan bir hafta sonra kallus oluşumunun meydana geldiği ve kayıp yaşanmadığı bildirilmiştir. Aşıdan 8-12 saat sonra anaç ve kalem vasküler dokuları arasında ilk kaynaşmanın sağlandığı, 20-30 gün sonra ise aşılarda tutmanın meydana geldiği bildirilmektedir (Cantos et al., 1995). ...
... Due to differences between growth and development phases within cultivars, the germination responses of embryos could be different (Linan et al., 1999). However, in this experiment cultivars showed similar response of seed germination percent and seedling formation percent in each harvesting time, therefore it can be concluded that embryo growth phases of these three cultivars were approximately similar. ...
... Previous works testing single cultivars grafted on a range of potential interesting rootstocks indicate that the intrinsic vigor of some rootstock is transferred to the grafted cultivar. However, opposite results were also found in some cases, although it should be noted that explicit evaluation of vigor of the rootstock used was not carried out but based on previous literature (Troncoso et al., 1990;Romero et al., 2014). Similarly, different vigor control ability has been obtained for some rootstocks depending on the cultivar grafted, while a general dwarfing effect was observed for some others (Pannelli et al., 2002). ...
... Anzani et al. 1990;Failla et al. 1992;Campostrini et al. 1993;Olmo 1995;Arnold et al. 1998;Ocete et al. 1999;Arnold 2002;Ocete et al., 2002Ocete et al., , 2011Ocete et al., , 2014Grassi et al. 2002;Grassi et al. 2003a, b;De Mattia et al. 2008;Di Vecchi et al. 2008;Arroyo-Garcia et al. 2006Zecca et al. 2010;Ergül et al. 2011;Myles et al. 2011;Biagini et al. 2012Biagini et al. , 2014Biagini et al. , 2016Bacilieri el al. 2013 Сосновский 1925, 1946; Вавилов 1931; Негруль 1946; მ. რამიშვილი 1948, 1958, 1961; Жуковский 1973; ფრუიძე 1974, 2016; ჩოლოყაშვილი 1983; Колаковский 1961; Чамагуа 1968; Р. Рамишвили, 1970, 1973,1988; რ. რამიშვილი 1978, 2001 ...
... Hence, In vitro propagation of endangered plants can offer considerable benefits for the rapid cultivation of species that are at risk, that have limited reproductive capacity and exist in threatened habitats (Fay, 1992). In vitro propagation methods are essential components of plant genetic resource management and they are becoming increasingly important for conservation of rare and endangered plant species (Sudha et al.,1998;Benson et al., 2000;Iankova et al., 2001;Bhatia et al., 2002).The Dendrobium fimbriatum is the second largest genus of Orchidaceae, which is composed of approximately 1500 species scattered in the world, and there is the most popular and highly valued orchids in the market (Chen and Ji, 1998). In vitro propagation of Dendrobium fimbriatum. ...
... Foliar applications of different fertilizers with or without N produce decreased total phenol and tocopherol contents in addition to changes in the profiles of minerals, sugars, phenols and tocopherols (Tekaya et al., 2014). High doses of nitrogen applied by fertigation in combination with phosphorus and potassium increase the fruit weight, fleshto-stone ratio, potassium and water contents but also degrade the pulp texture and decrease the total sugar content (Morales-Sillero et al., 2008). However, the extent to which all of these changes observed in table olives influence sensory properties or antioxidant activity is unknown. ...