A. Sokolenko's research while affiliated with Ludwig-Maximilians-University of Munich and other places

Publications (13)

Article
Photosynthetic bacteria contain one or two homologues of the signal peptide peptidase A Escherichia coli, designated SppA1 and SppA2. SppA2 protein is unique for prokaryotic cells and in cyanobacterium Synechocystis sp. PCC6803 (Synechocystis) is encoded by the gene slr0021. We have established that in cyanobacteria the transcriptional and post-tra...
Article
The genome of cyanobacterium Synechocystis sp. PCC 6803 contains the sll0136 (pepP) gene encoding the putative homolog of proline aminopeptidase PII (AMPPII) of the heterotrophic bacterium Escherichia coli. AMPPII is known to cleave the N-terminal amino acid residue of peptides and proteins only in the case of a penultimate proline position. The Sy...
Article
Full-text available
We describe the identification of a novel chloroplast protein, designated TCP34 (tetratricopeptide-containing chloroplast protein of 34 kDa) due to the presence of three tandemly arranged tetratricopeptide repeat (TPR) arrays. The presence of the genes encoding this protein only in the genomes of higher plants but not in photosynthetic cyanobacteri...
Article
Overwintering needles of the evergreen conifer Douglas fir exhibited an association between arrest of the xanthophyll cycle in the dissipating state (as zeaxanthin + antheraxanthin; Z + A) with a strongly elevated predawn phosphorylation state of the D1 protein of the photosystem II (PSII) core. Furthermore, the high predawn phosphorylation state o...
Article
The sll1703 gene, encoding an Arabidopsis homologue of the thylakoid membrane-associated SppA peptidase, was inactivated by interposon mutagenesis in Synechocystis sp. strain PCC 6803. Upon acclimation from a light intensity of 50 to 150 μE m−2 s−1, the mutant preserved most of its phycobilisome content, whereas the wild-type strain developed a ble...
Article
A homozygous insertion mutant with the inactivated clpP2gene, which encodes the proteolytic subunit of ATP-dependent peptidase, was obtained in the unicellular cyanobacterium Synechocystissp. PCC 6803. The mutant cannot grow under photoautotrophic conditions, but cells grown under heterotrophic conditions in a glucose-containing medium have active...
Article
Full-text available
A homozygous insertion mutant with the inactivated clpP2 gene, which encodes the proteolytic subunit of ATP-dependent peptidase, was obtained in the unicellular cyanobacterium Synechocystis sp. PCC 6803. The mutant cannot grow under photoautotrophic conditions, but cells grown under heterotrophic conditions in a glucose-containing medium have activ...
Article
We describe two related manganese-binding polypeptides with L-arginine metabolizing enzyme activity that can be detected as distinct components (designated PsbY-A1 and PsbY-A2, previously called L-AME) in membranes containing Photosystem II (PS II) from spinach. The polypeptides are bitopic and appear to exist in a heterodimeric form, but only in t...
Chapter
As „open systems” organisms do not only add compounds (influx of matter), they also constantly exchange matter with their environment to modify structures and functions for optimization, maintenance, acclimatization, stress protection, and repair. Molecular mechanisms controlling this efflux of matter must contribute with comparable quality to the...
Article
Full-text available
Chloroplast subfractions were monitored for sodium dodecyl sulfate-stable proteases. Nine distinct activities in the molecular mass range from 14 to 66 kD have been detected. Five of the proteases associated with thylakoid membranes belong to the serine and cysteine types of proteases. These activities could be preserved and purified by a two-step...

Citations

... Notwithstanding, some slight differences do exist between the actions of SppA1 and SppA2. While SppA1 is involved in photo-acclimation upon high light exposure, the Spp2 was projected to be involved at the recovery stage instead, when already the proteins that were required for photo-protection under high light stress were gathered and are perhaps not required any further (Pojidaeva and Sokolenko 2017). ...
... Upon switch of cells from high Cu 2+ to Cu 2+ -depleted growth medium, amounts of proteins involved in the tRNAs synthesis were restored to the standard expression levels but the elongation factor P remained significantly downregulated. In parallel to components of protein synthesis, aminopeptidase P (Sll0136), which is involved in protein degradation, especially in high light and heat conditions (Pojidaeva et al. 2013), was downregulated during the high Cu 2+ phase and further decreased its amount after switching to Cu 2+ -depleted medium. In contrast, the periplasmic peptidase YmxG (Slr1331) responsible for the maturation of periplasmic proteins (Fulda et al. 2000) was upregulated at the end of the experiment. ...
... Not only PSII repair but also the rate of the damaging reaction of photoinhibition decreases with temperature (Lazarova et al., 2014;Mattila et al., 2020Mattila et al., , 2022Tyystjärvi et al., 1994;Ueno et al., 2016), but it is not clear if photoinhibition of PSII occurs at temperatures below zero, or in a frozen environment. A decrease in the amount of the D1 protein has been observed during winter in evergreen spruce needles (Ebbert et al., 2005;Ensminger et al., 2004;Míguez et al., 2017;Ottander et al., 1995;Verhoeven et al., 2009) but the decrease might represent downregulation of photosynthesis rather than PSII damage. However, the accumulation of PSII complexes with properties linking them to the repair cycle has been observed during harsh winter conditions, suggesting ongoing photoinhibition (Grebe et al., 2020). ...
... The expression of gene PsbY was downregulated under high Zn stress whereas, the transcripts level of PsbY was increased in the addition of Si. PsbY is a polyprotein photosystem II a subunit of the oxygen-evolving complex of PSII, a manganese-binding polypeptide contains L-arginine metabolizing enzyme activity (Gau et al., 1998;Suorsa and Aro, 2007). It plays a vital role in the function of Mn 4 Ca cluster in oxygen-evolving photosystem II. ...
... The three ClpPs and ClpX are all highly induced by high light in both Synechocystis 6803 and Synechococcus 7942 (Mary et al. 2004;Schelin et al. 2002). A mutation of ClpP2 has been described as light-sensitive and prevents photoautotrophic proliferation of Synechocystis 6803 (Panichkin et al. 2001). In particular, the protein content of ClpP1 increased under UVB light and cold stresses in the long-term period (over an 8-to 24-h) and was involved in the stress adaptation in Synechococcus 7942 Porankiewicz et al. 1998). ...
... HvPAP14 is involved in the degradation of the lightharvesting Chl a/b-binding proteins and the large subunit of Rubisco (Frank et al., 2019). There is only one other report which has provided evidence of cysteine protease activity in the thylakoid lumen (Sokolenko et al., 1997). ...
... Few studies on Synechoscystis species have, however, focused on light preferences, especially Synechocystis sp. strain PCC 6803 [25,26]-a strain mainly composed of APC and PC [27]. S. salina LEGE 01655 was found to have PE as additional pigment in its phycobilisome [22], but a gap remains regarding how light influences its metabolic responses. ...
... SWEET11 is a sucrose exporter for phloem loading (Chen et al. 2012); it is also expressed in cells associated with xylem vessels and it can transport also glucose and fructose (Le Hir et al. 2015). TCP34 (tetratricopeptide-containing chloroplast protein of 34 KDa) can bind plastidic DNA (Weber et al. 2006;Kabeya et al. 2010;Majeran et al. 2012;Pfalz and Pfanneschmidt 2013). ...