A. N. Kalinina's research while affiliated with Kurchatov Institute and other places

... Previously, we described the prototrophic strain K. phaffii VKPM Y-4287 with high expression potential [17] and phytase from Citrobacter gillenii, which has industrially valuable characteristics [18]. The aim of this work was to develop recipient strains based on the K. phaffii VKPM Y-4287 with unchanged growth characteristics and expression potential using highly specific CRISPR/Cas9 system, as well as to investigate the possibility of sequential introduction of genetic material into the recipient chromosome to obtain a strain-producer with multiple copies of the C. gillenii phytase gene. ...

... Currently, the production of phytase by engineering strains has been achieved via high-density fermentation of recombinant P. pastoris and the specific activity with sodium phytate as a substrate is 1452 U/mg [1]. However, during traditional batch fermentation, continuous production is difficult to achieve because of frequent feeding and drawing [2,3]. ...

... Pichia pastoris is a significant research target in the field of pharmaceutical proteins. Compared with other existing expression systems, Pichia pastoris has obvious advantages in the processing of expression products, external secretion, posttranslational modification, and glycosylation modification, and has been widely used in the expression of exogenous proteins [1][2][3][4]. In order to give full play to Pichia pastoris expression system as an excellent protein expression system, maximize the production efficiency and product quality of Pichia pastoris fermentation to express exogenous proteins, and reduce the production cost [5,6], it is necessary to conduct dynamic regulation and real-time optimization of the fermentation process of Pichia pastoris, so as to accurately control the production under the optimal technological conditions. ...

... Similar activation effects by Mn 2+ ,Co 2+ , Zn 2+ , Ca 2+ , Mg 2+ of 10 mM has been described for xylanase from A. fumigatus JCM 10,253 [61]. The activation effect of Co 2+ or Mn 2+ on GH 10 xylanase Xyn27, AfXylA10 and 11 xylanase Xyl1 with reported [62][63][64], while they inhibited Caldicoprobacter algeriensis XynBCA [4]. It is pointed out that the effect of metal ions on enzyme activity is closely related to xylanase structure. ...

... Recently, several studies on soil proteomes have discovered new microorganisms that produce new glucanases. However, most of these studies are still restricted to the chemical and molecular identification of enzymes (Zhou et al. 2016;Angelov et al. 2017;Borshchevskaya et al. 2019;Edison and Pradeep 2020). However, dos Gonçalves et al. (2020) identified a β-glucanase in mangrove soils, which is thermostable and active at basic pH. ...

... The phytase activity of the selected transformants was determined on a diagnostic medium with CaCl 2 , according to the method Gordeeva et al. 2018. The transformants were grown at 30 • C for 24 h. ...

... To this end, Bedford (2000) explored the use of genetic engineering to improve exogenous enzyme thermal stability and adaptation to the high temperatures of the process of feed preparation. Gordeeva et al. (2019) increased the thermal stability of enzymes used in feedstuffs by sitedirected saturation mutagenesis. Genetic engineering has been widely used to improve enzyme properties to promote their better application. ...

... It exhibits maximum activity at pH 4.9, thermal stability up to 60 • C (at pH 3.0) and a maximum activity of~340 FYT/mg. There are reports about production of this enzyme in Pichia pastoris including its artificially modified derivative with enhanced thermal stability [22][23][24]. ...

... The expression vector pPIC9 [12], which contains the inducible AOX1 promoter, the S. cervisiae factor signal sequence, and the selective marker HIS4 was used. The phyS3-mod gene with an optimized composition was synthesized at the Kurchatov Institute NRC of GOSNIIGENETIKA with the method in [13]. ...

... The presence and amount of lactic acid in the solution were determined, as it reacted with iron(II) chloride forming yellowish-green iron(IIII) lactate. The absorbance of the obtained solution was measured using a wavelength of 390 nm and is proportional to the concentration of lactic acid in the sample [2]. ...