March 2013
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58 Reads
Objective: To assess biocompatibility of an hydrogel scaffold (BD-Matrigel) with MSCg in a tooth model in vitro. Method: Mesenchymal stem cells were isolated from gingival connective tissue, immunophenotype was characterized by flow citometry, using specific antibodies markers. MSCg were differentiated into adipocytes, osteoblasts and chondroblasts following the criteria established by the ISCT. MSCg were cultured with BD-Matrigel, the construct was implanted in a tooth that was instrumented with Mtwo (VDW) rotary instrumentation system, then the tooth was cultured for 10 days. Biocompatibility was assessed by viability test, proliferation curve and cell type identification with histological cuts. Result: It was observed that the whole BD-Matrigel cultivated with MSCg in the tooth model, permitted proliferation, cell viability and cell function. It also was demonstrated the presence of cellular elements within the construct, subsequent to cultivation. Conclusion: BD-Matrigel proved to be biocompatible with MSCg, when cultured and implanted into a tooth model in vitro.