A Corradi’s research while affiliated with University of Parma and other places

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Publications (59)


Investigation on epithelial to mesenchymal transition (EMT) of EcPV-2 positive equine vulvar squamous cell carcinoma: a spontaneous model for human genital carcinomas
  • Article

May 2023

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13 Reads

Journal of Comparative Pathology

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L. De Paolis

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Oxidative stress in canine histiocytic sarcoma cells (DH82 cells) induced by a persistent canine distemper virus infection leads to impairment of the HIF-1α downstream pathway in vitro
  • Poster
  • File available

March 2020

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90 Reads

Introduction: Histiocytic sarcomas represent malignant tumors, which require new treatment strategies (Abadie, 2009). One option might be viral oncolysis with canine distemper virus (CDV) as a promising candidate. This virus exhibits oncolytic features as reported for a canine histiocytic sarcoma cell line (DH82 cells) (Beineke, 2009; Lapp, 2014). Interestingly, according to the literature the underlying mechanism might include also a dysregulation of angiogenesis (Pfankuche, 2017). Based on these findings, the aim of the present study was to investigate the impact of a persistent CDV‐infection on oxidative stress mediated changes in the expression of hypoxia‐inducible factor (HIF)‐1α and its angiogenic downstream pathway in DH82 cells in vitro. Materials and Methods: Non-infected (DH82 cells) and persistently CDV (strain Onderstepoort) infected canine histiocytic sarcoma cells (DH82Ond pi cells) were investigated with Flow cytometry to determine the oxidative burst within cells. Furthermore, microarray analysis was performed for comparative investigation of genes involved in ROS production/scavenging and the HIF-1α pathway. After this, five formalin-fixed, paraffin-embedded (FFPE) cell pellets of non-infected DH82 and DH82Ond pi cells were subjected to immunofluorescence (IF) for 8‐hydroxyguanosine (8OHdG), superoxide dismutase 2 (SOD2), catalase, HIF-1α, vascular endothelial growth factor-B (VEGF-B), canine distemper virus-nucleoprotein (CDV-NP; Tab.1). In order to quantify HIF-1α production, immunoblotting was used. The aberrant localization of HIF-1α was investigated with immunoelectron microscopy and laser scanning confocal microscopy combined with 3D modeling of living cells analyzing z-stacks with LAS X 3D Version 3.1.0 software from Leica. Statistical analysis was carried out using GraphPad Prism (version 8.0.1). Results: Persistent CDV infection of DH82 cells led to an increased level of intracellular ROS as assessed by flow cytometry (Fig.1) and microarray analysis (Fig.2) associated with increased catalase and SOD2 protein expression (Fig.3). DH82Ond pi are characterized by an increased HIF-1α protein expression (IF, Fig.4; immunoblotting, Fig.5) associated with an altered intracellular distribution. Immunoelectron microscopy (Fig.6) and the 3D digital reconstructions of living cells (Fig.7) revealed an unusual, mainly sub-membranous, distribution of HIF-1α. The unexpected intracellular localization is associated with a dysregulated expression of angiogenetic downstream targets as assessed by microarray analysis (Fig.2) and IF (Fig.8). Conclusion: Summarized, the results of the current in vitro study are indicative of a reduced activation of the HIF‐1α angiogenetic downstream pathway in DH82 cells persistently infected with CDV‐Ond compared to non‐infected controls. This is most likely due to an excessive, unusually localized, and non‐functional expression of HIF‐1α, which might be the consequence of a decreased cytosolic degradation of this transcriptional factor following a virus‐induced increased oxidative stress. Future studies are warranted to better characterize the presence of an increased oxidative stress and an aberrant HIF‐1α localization in DH82Ond pi also in vivo. The latter approach might further substantiate the assumed correlation between reduced angiogenesis, hypoxia and tumor regression in DH82Ond pi xenotransplants.

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Citations (27)


... Investigations of MET in sarcomas in canine tumors remains an open field in veterinary medicine. To date, only data from preliminary studies on canine perivascular wall tumors are available [354]. ...

Reference:

An Overview of Epithelial-to-Mesenchymal Transition and Mesenchymal-to-Epithelial Transition in Canine Tumors: How Far Have We Come?
Mesenchymal to Epithelial Transition in Canine Perivascular Wall Tumours (PWTS): Preliminary Assessment
  • Citing Article
  • February 2022

Journal of Comparative Pathology

... The necropsy of all animals involved did not show any specific macroscopic lesions, whereas histophatological findings showed interstitial pneumonia foci and hyperplasic fea- (Ferrari et al., 2003). In conclusion our results show that under experimental conditions different strains of PRRSV, with genetic differences in ORF 5, have mild virulence and are able to stimulate a similar immune response among the different strains, while the histophatology highlights large differences in their aggressiveness. ...

Experimental infection with a Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) strain with relevant genomic mutations in the ORF5 region

... A cistomatose é descrita em literatura como rara em felinos, no entanto, alguns autores sugerem que algumas doenças de ouvido médio têm sido subdiagnosticadas e recomendam a realização de avaliação histopatológica de lesões sempre que possível (Cantoni et al., 2018). ...

Gross Evaluation, Sampling Procedures and Histological Characterization of Feline Middle Ear Diseases
  • Citing Article
  • January 2018

Journal of Comparative Pathology

... 3 Studies showed an almost complete release of 3-MCPD from its esterified form 4,5 in the organism and thus esters of 3-MCPD and free 3-MCPD produced similar effects on the target organs at equimolar doses. 3,6 Although the toxicity of MCPD is not yet fully understood, the effect of 3-MCPD in the testes and kidneys is associated with its oxidative metabolism to β-chlorolactaldehyde and βchlorolactic acid. 7 The International Agency for Research on Cancer (IARC) classified 3-MCPD as possibly carcinogenic to humans (group 2B). ...

Comparison between 3‐MCPD and its palmitic esters in a 90‐day toxicological study
  • Citing Article
  • September 2011

EFSA Supporting Publications

... male antifertility effects), as well as non-genotoxic carcinogenic effects (e.g. renal tubular tumors) in rodents (Barocelli, Corradi, Mutti, & Petronini, 2011;Cho, Han, Lee, et al., 2008;Sunahara, Perrin, & Marchessini, 1993). ...

90-day toxicological study of 3-MCPD and its dipalmitate SCIENTIFIC REPORT submitted to EFSA "Comparison between 3-MCPD and its palmitic esters in a 90-day toxicological study" 1 Prepared by

... As articulações do ovino são modelos promissores para a investigação dos estados normais e patológicos, pela semelhança com a mecanobiologia de determinadas articulações humanas e pela susceptibilidade a doenças articulares (DAWSON, 1987;APPLEYARD et al., 1999;HOLY et al., 2000;CALLADO et al., 2001;TAPPER et al., 2006;SPADARI et al., 2013). ...

Effects of intraarticular treatment with stanozolol on synovial membrane and cartilage in an ovine model of osteoarthritis

Research in Veterinary Science

... In this study, an overall positive correlation was found between cortisol and GH but not between GH and IGF-1 plasma levels; the mean GH plasma level was lower in T1 at day 35. These observations furtherly sustain an uncoupling between the plasmatic levels of GH and IGF-1 and that the level of plasmatic GH could be mainly involved to counteract the levels of cortisol and their negative effect on metabolism and on immune efficiency in weaned piglets (Borghetti et al., 2006aBorghetti et al., , 2006bBorghetti et al., and 2009). In this view, the different trend in mean cortisol and GH plasma levels between groups could testify a reduction of the negative effect of stress on growth in T1. ...

Effect of Weaning and Vaccinations on Immune and Hormonal Parameters in Neonatal Piglets

Veterinary Research Communications

... NPTr cells (38) were cultured in Minimum Essential Medium (MEM, Eurobio scientific) supplemented with 10% FCS and 1% PSA, and incubated at 37°C in 5% CO 2 . ...

Establishment and characterization of two new pig cell lines for use in virological diagnostic laboratories
  • Citing Article
  • March 2003

Journal of Virological Methods

... One study evaluated the efficacy of four DNA vaccines against BoHV-1 in calves. The findings revealed that after 90 days of the initial vaccination, all calves were challenged with BoHV-1, resulting in severe IBR infection in every calve (125). Another study highlighted that the BoHV-1 DNA vaccine, encoding the gC and VP8 antigens, did not provide virologic protection compared to control groups, indicating the limitations of DNA vaccines in large animals and their reduced potency (123). ...

Evaluation of safety and efficacy of DNA vaccines against bovine herpesvirus-1 (BoHV-1) in calves
  • Citing Article
  • November 2009

Comparative Immunology Microbiology and Infectious Diseases

... Immune cells are sensitive to T-2 toxin and DON treatment (Ferrari et al. 2009;Chen et al. 2019). Exposure to these toxins increases pro-inflammatory cytokine expression Liu et al. 2019b), and T-2 toxin exposure decreases CD4 + T cell abundance and causes immunotoxicity (Taylor et al. 1985). ...

Cellular immune response and immunotoxicity induced by DON (deoxynivalenol) in piglets

Veterinary Research Communications