A. Bertaccini's research while affiliated with University Hospital of Parma and other places
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Publications (289)
This is a case of [68 Ga]Ga-Prostate-specific membrane antigen (PSMA)-11 PET/CT in a 73-years old patient presenting high Prostate Specific Antigen (PSA) levels despite both multi-parametric magnetic resonance imaging (mpMRI) and 12-core saturation biopsy negative for prostate cancer (Pca). This is a highly interesting case because, despite the adv...
Background and Objectives: The aim of this article is to present a single-surgeon, open retroperitoneal lymph node dissection (RPLND) series for testicular cancer in a high-volume center. Materials and Methods: We reviewed data from patients who underwent RPLND performed by an experienced surgeon at our institution between 2000 and 2019. We evaluat...
Objective
to evaluate the impact of 3D model for a comprehensive assessment of surgical planning and quality of partial nephrectomy (PN).
Materials and methods
195 patients with cT1-T2 renal mass scheduled for PN were enrolled in two groups: Study Group (n= 100), including patients referred to PN with revision of both 2D computed tomography (CT) i...
Symptoms of bud proliferation and aborted seed pods were observed in Iranian soybean fields in Golestan and Mazandaran provinces. Symptomatic soybean plants, possible insect vectors, and alternative plant host species were collected to verify the presence of possible pathogens. Symptomatic and asymptomatic soybean and herbaceous plant samples were...
Objective
Local tumor ablation to treat small renal mass is increasing. The aim of the present study was to compare oncologic outcomes among patients with T1 renal mass treated with partial nephrectomy and local tumor ablation.
Methods
To reduce the inherent differences between patients undergoing laparoscopic or robot-assisted partial nephrectomy...
Background: The aim, of this study was to investigate recurrence rates in patients with T1 renal cell carcinoma (RCC) undergone partial nephrectomy (PN), radiofrequency ablation (RFA) or cryoablation (Cryo). Methods: We retrospectively evaluated data from 665 (81.4%), 68 (8.3%) and 83 (10.3%) patients who underwent PN, RFA and Cryo, respectively. K...
Objective:
3D models are increasingly used as additional preoperative tools for renal surgery. We aim to evaluate the impact of 3D renal models in the assessment of PADUA, RENAL, Contact Surface Area (CSA) and Arterial Based Complexity (ABC) for the prediction of complications after Robot assisted Partial Nephrectomy (RAPN).
Methods and materials...
Background
Three-dimensional (3D) models improve the comprehension of renal anatomy.
Objective
To evaluate the impact of novel 3D-derived parameters, to predict surgical outcomes after robot-assisted partial nephrectomy (RAPN).
Design, setting, and participants
Sixty-nine patients with cT1-T2 renal mass scheduled for RAPN were included. Three-dim...
Background
Minimally-invasive approach is one of the mainstays of Enhanced Recovery After Surgery (ERAS) pathways. Robot-assisted radical cystectomy (RARC) introduction has reduced the surgical burden on patient's recovery. Accordingly, ERAS protocol benefits may be more striking in RARC patients. We evaluated the impact of surgical approach on per...
Background:
We aimed to assess the detection rate of overall PCa and csPCa, and the clinical impact of MRI/TRUS fusion targeted biopsy (FUSION-TB) compared to TRUS guided systematic biopsy (SB) in patients with different biopsy settings.
Methods:
305 patients were submitted to FUSION-TB, divided into three groups: biopsy naïve patients, previous...
Background
Incidental Prostate cancer (iPCa) is a relatively common finding during histopathological evaluation of radical cystectomy (RC) specimens. To reduce the high impact of RC on erectile function, several sexual-preserving techniques have been proposed. The aim of this study was to evaluate and compare the oncologic outcomes of patients with...
Background:
To investigate recurrence rates in patients with T1 renal cell carcinoma (RCC) undergone partial nephrectomy (PN), radiofrequency ablation (RFA) or cryoablation (Cryo).
Methods:
We retrospectively evaluated data from 665 (81.4%), 68 (8.3%) and 83 (10.3%) patients who underwent PN, RFA and Cryo, respectively. Kaplan-Meier curves depic...
Background:
We aimed at comparing perioperative outcomes in patients submitted to radical cystectomy followed by Fast Track (FT) protocol or standard management, and propose a definition of Trifecta, to improve standardized quality assessment for RC.
Methods:
We considered 191 patients submitted to RC between January 2017 and January 2019. Patie...
Between 2016 and 2017, symptoms of witches’ broom, proliferation, drying of twigs and ball-like structures formation were observed in Pinus brutia in urban green areas and parks in Isfahan (Iran). All samples from affected P. brutia trees resulted positive in nested-PCR using primer pairs targeting the phytoplasma 16S rRNA gene. Amplicons were obta...
Objective: To assess the economic impact of Holmium laser enucleation of prostate (HoLEP) in comparison with transurethral resection of prostate (TURP) and open prostatectomy (OP).
Methods: Between January 2017 and January 2018, we prospectively enrolled 151 men who underwent HoLEP, TURP or OP at tertiary Italian center, due to bladder outflow obs...
Grapevine yellows (GY) are an important threat to grapevine industry in Europe. In Italy, GY are mostly represented by Flavescence dorée (FD) and Bois noir (BN), respectively associated with infection by 16SrV and 16SrXII phytoplasma ribosomal groups. In the frame of a regional strategy aimed to monitor GY presence, an extensive survey was carried...
Sophora alopecuroides dwarfing and yellowing symptoms were observed at the edge of several Damaneh and Fereydun Shahr (Isfahan province, Iran) fields. Polymerase chain reaction using primers P1/P7 and nested PCR using the same primers followed by R16mF2/R16mR2 and R16F2n/R16R2 primer pairs amplified the 16S ribosomal RNA-encoding gene in all the sy...
Introduction: to evaluate the impact of 3D digital reconstructions of renal models on the arterial clamping approach during partial nephrectomy (PN).
Patients and Methods
57 patients with T1 renal mass referred PN, were prospectively enrolled in two groups: Group 1 (n= 32) with revision of both 2D computed tomography (CT) imaging and 3D virtual mo...
Background:
To develop a clinical nomogram aimed to predict the achievement of trifecta in patients treated with open, laparoscopic and robotic partial nephrectomy (PN) for localized renal masses ( < cT2).
Methods:
We retrospectively evaluated 482 consecutive patients who underwent PN with open (OPN: 243), laparoscopic (LPN: 156) and robotic (RA...
Objective
To evaluate the clinical impact of ⁶⁸Ga‐prostate‐specific membrane antigen positron emission tomography/computed tomography on the planned management of prostate cancer patients with biochemical recurrence after surgery.
Methods
We enrolled 276 prostate cancer patients referred to ⁶⁸Ga‐prostate‐specific membrane antigen positron emission...
Flavescence dorée (FD) is a grapevine quarantine disease associated with phytoplasmas and transmitted to healthy plants by insect vectors, mainly Scaphoideus titanus. Development of efficient methods for its control has been hampered by the lack of knowledge about phytoplasma biological properties, linked also to difficulties in its in vitro cultiv...
Leaf and branch samples of 10 Populus nigra L. cv. Italica trees were collected from the urban Zagreb area in late summer/autumn 2001. One of the trees exhibited leaf yellowing, overall sparse foliage, stunting and decline. Phytoplasma 16S rDNA was amplified in direct and nested PCR assays using universal and specific phytoplasma primer pairs, from...
Different ornamental plants showing symptoms indicating phytoplasma presence were collected between 1993 and 2016 in various floricultural areas in north and south of Italy, including Sicily. These samples were determined to be infected by ‘Candidatus Phytoplasma asteris’-related strains, and after PCR/RFLP identification based on the 16S rRNA gene...
Several molecular markers are currently available for phytoplasma strain discrimination. However, these markers often cannot be used for identification of phytoplasmas belonging to different ribosomal groups, or are not suitable for routine diagnostics. The DNA barcode amplicon based on the elongation factor Tu (tuf) gene for universal phytoplasma...
Background: Prostate biopsy is the gold standard for prostate cancer (PCa) diagnosis, but it's invasive and associated with adverse events. Novel reliable tumor biomarkers and accurate non-invasive tests are required to avoid biopsies. The immune complex PSA-IgM is a new marker for PCa, and it has been included in an algorithm to generate the diagn...
Aim:
To present a summary of the updated guidelines of the Italian Prostate Biopsies Group following the best recent evidence of the literature.
Materials and methods:
A systematic review of the new data emerging from 2012-2015 was performed by a panel of 14 selected Italian experts in urology, pathology and radiology. The experts collected arti...
Surveys for the Côte d'Ivoire lethal yellowing (CILY) phytoplasma were conducted in eight severely CILY-affected villages of Grand-Lahou in 2015. Leaves, inflorescences and trunk borings were collected from coconut palms showing CILY symptoms and from symptomless trees. Total DNA was extracted from these samples and tested by nested polymerase chai...
Onobrychis viciifolia (common sainfoin) is a forage legume which enhances biodiversity within agro-ecosystems and is an important forage crop in Iran. During 2014-16, O. viciifolia plants were observed with symptoms of yellowing (Fig. 1), little leaf and reddening of leaflet margins (Fig. 2), witches’ broom and dwarfing compared with healthy plants...
Calendula officinalis , commonly known as marigold, belongs to the family Asteraceae. In Iran, this species is cultivated for ornamental purposes and used for many medicinal, culinary and cosmetic uses. From 2006, Calendula officinalis …
Trillium (family Melanthiaceae) is a genus of perennial flowering plants native to temperate regions of North America and Asia, and traditionally used as medicinal plants (Case, 2000). There are 49 species within the genus Trillium amongst…
During 2010–14 surveys in the major sesame growing areas of Fars, Yazd and Isfahan provinces (Iran), genetic diversity and vector transmission of phytoplasmas associated with sesame phyllody were studied. Virtual RFLP, phylogenetic, and DNA homology analyses of partial 16S ribosomal sequences of phytoplasma strains associated with symptomatic plant...
The focus of this research was the development and evaluation of different complex liquid and solid media for the isolation and growth of phytoplasma strains infecting grapevine plants. Previously reported media supporting phytoplasma isolation are commercial and not easy to modify in order to improve performance and selectivity towards obtaining p...
In 2014-15 surveys, alfalfa witches' broom (AWB) disease was observed in a number of Iranian provinces. The main symptoms were crown proliferation, witches' broom, little leaf, flower virescence, phyllody, sterility and stunting. To investigate the phytoplasma presence, total DNAs were extracted from 132 symptomatic and 19 asymptomatic alfalfa plan...
Coconut farms located in the southern coast of Grand-Lahou in Côte d’Ivoire are severely affected by a lethal yellowing disease (CILY) associated with the group 16SrXXII-B, ‘Candidatus Phytoplasma palmicola’-related strains. Given the high prevalence of weed species on most of the farms, plants growing within and in the periphery of five selected c...
During 2011-14 surveys, a witches’ broom (CarWB)
disease was observed in carrot fields of Chahgeer and Salim
Abad in Abarkooh and Khatam regions (Yazd province,
Iran). The main symptoms of CarWB disease were
little leaf, yellowing, proliferation of shoots from taproot,
stunting of taproot, virescence, phyllody, leaf reddening
and witches’ broom. Th...
Grapevine crown gall caused by Allorhizobium vitis, or less frequently by Agrobacterium tumefaciens complex and Rhizobium rhizogenes, may seriously impact production in nurseries and vineyards worldwide. Although rapid and efficient detection and identification of tumorigenic bacteria is facilitated by PCR-based methods, high genetic diversity of t...
Medicinal and aromatic plants include a broad array of wild and cultivated plants which contain many biologically- active compounds, known as phytochemicals, that are of great interest for their ability to promote human and animal health. The present review provides a literature overview of phytoplasma diseases affecting medicinal and aromatic plan...
Alfalfa witches' broom (AWB) is a devastating disease distributed mainly in tropical provinces of Iran and widespread all over the Sistan-Baluchestan province. The highest incidence was found in Rask (Sarbaz) (up to 70%), Sefidaba (Zabol) (up to 46.26%), Sarboog (Nikshahr) (up to 45.4%), Mirjaveh (Zahedan) (up to 40%), Shams Abad (Saravan) (up to 3...
Alfalfa witches' broom (AWB) is a devastating disease distributed mainly in tropical provinces of Iran and widespread all over the Sistan-Baluchestan province. The highest incidence was found in Rask (Sarbaz) (70%), Sefidaba (Zabol) (46.26%), Sarboog (Nikshahr) (45.4%), Mirjaveh (Zahedan) (40%), Shams Abad (Saravan) (32.2%), Namardian (Iranshahr) (...
Aims:To analyze genetic diversity and epidemiological relationships among 54 strains of Allorhizobium vitis isolated in Europe during an eight-year period and to assess the relative contribution of mutation and recombination in shaping their diversity.
Methods and results: By using random amplified polymorphic DNA (RAPD) PCR, strains studied were d...
Erysimum linifolium, Aegean wallflower, is an evergreen perennial compact shrub mostly cultivated to be used in rock gardens or in mixed garden borders. A phytoplasma-like disease was observed in pot plants at an ornamental grower in northern Italy. Symptomatic E. linifolium showed reduced leaf size, rosetting and stunting; in some cases, shortenin...
Two genera of the Fabaceae family showed phytoplasma symptoms in Germany and in Italy. Spartium junceum showing typical SpaWB symptoms was observed in the city of Ercolano (Campania region, Italy) and a similar symptomatology was observed in a group of shrubs of Cytisus scoparius growing in Dahlem botanical garden in Berlin (Germany). Symptomatic a...
Epidemiological surveys were carried out in Liguria and Lazio regions to detect virus and phytoplasma infections in Hydrangea machrophylla. Plants showing stunting, flower virescence and phyllody, yellowing, necrosis and redness of the leaf edge, collected in "Piana di Albenga" (Liguria region), were infected by Hydrangea ringspot virus. One of the...
Purpose:
The management of mildly elevated (4.0-10.0 ng/ml) prostate specific antigen (PSA) is uncertain. Immediate prostate biopsy, antibiotic treatment, or monitoring PSA level for 1-3 months is still in controversy.
Materials and methods:
We retrospectively analysed the effect of empiric antibiotics on an increased PSA in a mono-institutional...
Tomato (Solanum lycopersicum) is an important vegetable in Cuba and worldwide. In 2009, a disease suspected to be associated with phytoplasmas was observed in tomato fields in Mayabeque, in the locality of San Jose in the western region of the country. Symptoms most frequently displayed were shortened internodes and stunting, chlorosis and reduced...
Background/Aim: The Urological Clinic of the S. Orsola-Malpighi University Hospital, Bologna has been carrying out laparoscopic radical prostatectomy since 2002. In this study, we report the results after 10 years of LRP, analyzing in particular the oncological and functional aspects.
Between March 2002 and August 2011, 400 patients underwent lapar...
An island wide survey carried over 18 months across 79 tomato (Lycopersicum esculentum L.) plantations in 30 localities on 12 varieties revealed that symptoms related to phytoplasma-associated diseases are widespread in this species in Mauritius. Typical witches, broom and stunting and/or yellowing were observed in 74.6 % of fields visited. Phytopl...
The rapid spreading of the disease during last few years highlighted the need of a quick, sensitive and reliable method for Pseudomonas syringae pv. actinidiae (Psa) detection, to find possible inoculum sources and limit the pathogen spreading. A PCR method, using new primers designed on the gene encoding a putative outer membrane protein P1, was d...
Five-year-old sweet cherry (Prunus avium L.) trees, exhibiting symptoms typical of phytoplasma diseases were observed in the Turkish province of Usak during 2011. The percentage of symptomatic plants, scattered in the orchards, was nearly 40%. Samples were collected during late spring and early summer from trees showing proliferation of branches, o...
Objectives:
Urine cell-free (UCF) DNA has recently been proposed as a potential marker for early bladder cancer diagnosis. It is known that normal apoptotic cells produce highly fragmented DNA while cancer cells release longer DNA. Therefore, we verified the potential role of UCF DNA integrity in early bladder cancer diagnosis.
Materials and meth...
Men affected with Benign Prostate Hyperplasia (BPH) and Lower Urinary Tract Symptoms (LUTS) are demonstrating to require an increasing amount of attention from Urologists and Primary-care Physicians. Over the years, common urological medications were based on either alpha-blockers and/or 5alpha-reductase inhibitors. During the last decade the phyto...
Hormonal therapy is one of the treatment options for prostate cancer patients. There are many
hormonal treatments modality to block the testosterone effect on prostate cancer cell proliferation. Degarelix is an innovative molecule able to antagonize the GnRH receptor with comparable oncological results to GnRH agonist, but with less side effects,...
Phytoplasmas are obligate intracellular bacterial parasites restricted to the phloem sieve
elements of the infected plants and are transmitted by phloem‐sucking insects belonging
to the families Cicadellidae, Cixidae, Psyllidae, Delphacidae and Derbidae. They are
associated with diseases of several hundred plant species, including some economica...
Prostate specific antigen (PSA) is still the best available tumour marker in prostate cancer (PCa), but presents some limits. Therefore, there is a need for novel markers in the detection and management of PCa. The 80-kDa soluble form of E-cadherin (sE-cad) and the cytokine IL-6 are being discussed as supplemental serum markers for PCa. In this stu...
The pigeon pea witches'-broom phytoplasma group (16SrIX) comprises diverse strains that cause numerous diseases in leguminous trees and herbaceous crops, vegetables, a fruit, a nut tree and a forest tree. At least 14 strains have been reported worldwide. Comparative phylogenetic analyses of the highly conserved 16S rRNA gene and the moderately cons...
Prunus and Pyrus species affected with phytoplasma diseases, as well as leafhopper species collected from Prunus and Pyrus fields in Ontario, Canada were tested for presence of phytoplasmas. Preliminary results showed that Graminella nigrifrons is a potential vector for phytoplasma groups 16SrI-W (‘Candidatus Phytoplasma asteris’), and 16SrVII-A (‘...
Ranunculus asiaticus L. is an ornamental species grown in many areas of the world for tuber, potted plant, and cut flower production. In Italy, the crops of Ranunculus hybrids are mainly located in the Liguria region, where virus diseases represent a serious problem in cut flower production. However, the impact of virus infections on the crop has n...
To evaluate the efficacy of contrast-enhanced ultrasound (CEUS) in patients with acute scrotal pain not defined at ultrasound (US) with colour Doppler .
CEUS was carried out in 50 patients with acute scrotal pain or scrotal trauma showing testicular lesion of undefined nature at US. The accuracy of US and CEUS findings versus definitive diagnosis (...
Population screening for prostatic carcinoma (CP) is a debated topic, and its real utility is still unknown. Nowadays only surveys on little groups or clinical randomized studies are recommended. In this study the male population of blood donors of our hospital aged between 45 and 65 years underwent a complete evaluation for CP. The objectives of t...
Phytoplasma classification established using 16S ribosomal groups and ‘Candidatus Phytoplasma’ taxon are mainly based on the 16S rDNA properties and do not always provide molecular distinction of the closely related strains such as those in the aster yellows group (16SrI or ‘Candidatus Phytoplasma asteris'-related strains). Moreover, because of the...