A. A. Budarin’s scientific contributions

The aim of the research is to develop a methodology for a comprehensive assessment of the decorativeness of landscape gardening roses for Russian wet subtropics. This research is based on the study of the biological characteristics of culture in this region. The thorough study of the complex of quantitative and qualitative characteristics of the garden rose’s assimilation apparatus is needed to develop a better classification under conditions of Russian wet subtropics. Then the allocation of the most important ones is necessary. The roundness index is the more accurate characteristic of the variety among the morphological parameters of the leaf. It has a smaller variation in comparison with the initial parameters of the leaf. The contents of chlorophyll a and the sum of carotenoids are more accurate characteristics of the variety among the qualitative indicators of the leaf. The calculated sign is the ratio of the sum of green and yellow pigments.

The increased activity of enzymes that counteract the oxidative degradation of substances is one of the physiological and biochemical adaptations of plants to biotic and abiotic factors. The aim of the research was to study the peculiarities of the antioxidant system of garden roses to elaborate a methodology for a comprehensive evaluation of culture in the wet subtropics of the Russian Black Sea coast. The objects of study were garden rose varieties ( Rosa × hybrida hort.), belonging to functional groups – bush and large-flowered, and differing in the degree of resistance to the main fungal diseases of the culture in the region ( Diplocarpon rosae FA Wolf, Podosphaera pannosa (Wallr.) De Bary, Botrytis cinerea Pers., Phragmidium mucronatum (Pers.) Schltdl.) The activity of the antioxidant system was evaluated by the activity of the enzymes catalase and peroxidase (guaiacol). Catalase activity (CAT) in physiologically mature rose leaves was determined by the gasometric method according to I. I. Gunar, peroxidase (GPO) by the modified method according to I. P. Ermakov, according to the absorption spectrum (wavelength – 440 nm), recorded on the spectrophotometer PE-5400vi. The studies were carried out in dynamics during the growing season in 2016-2017. The article presents data on changes in the activity of enzymes of the primary antioxidant system – catalase and peroxidase, in the leaves of bush and large-flowered varieties of garden roses, which differ in the degree of resistance to the main phytopathogens of the culture in the region. Variety differences in the activity of enzymes, the dynamics of changes in the activity of antioxidant enzymes during the growing season, the dependence of the enzymatic activity on the hydrothermal conditions of the humid subtropical climate of the Sochi Black Sea Region have been determined. Diagnostic parameters have been established to develop the methodology for a comprehensive evaluation of landscape gardening roses in the region. When developing methods for a comprehensive evaluation of garden roses, the activity of guaiacol peroxidase can be used to analyze the adaptive potential of the culture in the region.