A. A. Batalova's research while affiliated with Sechenov Institute of Evolutionary Physiology and Biochemistry and other places

Publications (8)

Article
Full-text available
Serum albumin possesses esterase and pseudo-esterase activities towards a number of endogenous and exogenous substrates, but the mechanism of interaction of various esters and other compounds with albumin is still unclear. In the present study, proton nuclear magnetic resonance (1H NMR) has been applied to the study of true esterase activity of alb...
Article
Full-text available
Being one of the most abundant proteins in human and other mammals, albumin plays a crucial role in transporting various endogenous and exogenous molecules and maintaining of colloid osmotic pressure of the blood. It is not only the passive but also the active participant of the pharmacokinetic and toxicokinetic processes possessing a number of enz...
Article
Full-text available
As a carrier of many biologically active compounds, blood is exposed to oxidants to a greater extent than the intracellular environment. Serum albumin plays a key role in antioxidant defence under both normal and oxidative stress conditions. This review evaluates data published in the literature and from our own research on the mechanisms of the en...
Article
The effect of the Cys34 oxidation state in human serum albumin on its binding and catalytic activity towards paraoxon was investigated using molecular modeling methods. Three levels of the cysteine oxidation were considered: (1) Cys34 is reduced, (2) Cys34 is oxidized to sulfenic acid, and (3) Cys34 is oxidized to sulfinic acid. The conformational...
Article
One of the urgent tasks of clinical toxicology is the development of therapy aimed at stoichiometric and/or catalytic detoxification of organophosphorus compounds in the bloodstream, which will prevent the poison’s entering the neuromuscular and neuronal synapses and help to avoid irreversible consequences of poisoning. An auxiliary option for the...
Article
The directional effect on albumin of the molecules which modulate its binding and/or esterase activity could allow stoichiometric and/or catalytic detoxification of organophosphates in the bloodstream. In this study, the effect of polyphenols on binding and catalytic activity of human (HSA) and rat (RSA) serum albumins has been investigated by mole...

Citations

... Albumin is a high-molecular-weight serum protein that helps lipids, hormones, and inorganic ions flow about the body. Albumin helps the body filter fluids and maintain colloidal blood osmotic pressure [89]. Hyperalbuminemia was discovered in the current investigation as a result of exposure to Ag-NPs. ...
... We have previously shown that Sudlow I and Cys34 sites in HSA and BSA molecules mutually influence each other; a change in the conformation of one leads to conformational changes in the other [60,61]. At the same time, in the redox site, the main role in reciprocal regulation is played by amino acid residues Cys34, His39 and Tyr140 (139), and their mutual arrangement; the position of the thiol group of the cysteine and the hydroxyl group of the tyrosine relative to the imidazole ring of His39. ...
... Enzymes ampersand non-enzymatic substances in the body assume the role of scavenging excess reactive oxygen species in the antioxidant defense system. SOD, CAT, and GSH-Px in the enzymatic antioxidant system are essential to maintaining oxidative homeostasis in the body (26). Superoxide anion radicals are turned into hydrogen peroxide by SOD during disproportionation, and then GSH-Px and CAT combine to break down the hydrogen peroxide into non-toxic substances (27). ...
... In in silico experiments investigating the interaction of albumin with OP, the influence of HSA redox status on their interaction with paraoxon was studied [77]. According to the data obtained, the redox status of Cys34 has no significant effect on the possibility of esterase reactions at Sudlow site I. ...
... In our recent study [88], molecular dynamics simulations were used to investigate geometrical characteristics of paraoxon complexes with Sudlow sites I and II of HSA in the presence and absence of oleic acid in sites FA5 and FA2 (sites with the highest affinity to oleic acid). Stability of productive complexes (those where attack of the tyrosine hydroxyl on the ligand's phosphorus is possible) and free energies of complex formation were evaluated. ...
... In the absence of experimental data, the threedimensional structure of the protein can be constructed using homologous modeling, which is the procedure of building a 3D-model of a protein from its primary sequence and the known three-dimensional structures of homologous proteins [51]. For the first time, using this method on the basis of 14 structures of homologous proteins, we constructed a three-dimensional RSA model [52], which was then tested in in silico experiments [53]. ...