Koji Kobayashi's research while affiliated with Chiba University and other places
Publications (4)
Cultured epithelium has been used successfully in the treatment of extensive burns. Regenerated epidermis, however, lacks such as hair follicles and sweat glands that are common in mammalian skin. We attempted to determine whether cultured epithelium could be induced to form hair follicles by dermal papillae, which are most important for the morpho...
The upper region of the outer root sheath of vibrissal follicles of adult mice contains multipotent stem cells that respond to morphogenetic signals to generate multiple hair follicles, sebaceous glands, and epidermis, i.e., all the lineages of the hairy skin. At the time when hair production ceases and when the lower region of the follicle undergo...
Rat vibrissa follicle morphogenesis starts around 13 days of gestation. By day 14 mesenchymal cells have already aggregated as 'condensations' beneath the initial hair bud. Some of the mesenchymal cells will form a dermal papilla, having profound effects on hair follicle formation. The appearance of follicle-inducing mesenchymal cells in the proces...
We have examined the growth capacity of keratinocytes isolated from human scalp hair follicles. Like the keratinocytes of glabrous epidermis, most of the colony-forming cells are classified as holoclones or meroclones when analyzed in a clonal assay. Some of them have extensive growth potential, as they are able to undergo at least 130 doublings. T...
Citations
... Frozen keratinocytes were thawed and cultivated at clonal density on a feeder layer of irradiated or mitomycin C-treated mouse 3T3-J2 fibroblasts. The co-culture was incubated at 37 °C with 10% CO 2 in a 3:1 mixture of Dulbecco-Vogt modification of Eagle's medium (DMEM) and Ham's F12 medium, supplemented with 10% fetal bovine serum (FBS), 1.8 × 10 −4 M adenine hemisulfate salt, 5 μg/ml insulin, 0.4 μg/ml hydrocortisone, 10 −10 M cholera toxin, and 2 × 10 −9 M triiodothyronine (T3), as described previously 1,30 . Keratinocytes between passages 2 and 7 were used for experiments, and the medium was changed every 4 days. ...
... The bulge area located at the level of insertion of the arrector pili muscle is considered to be a site of pluripotent epithelial stem cells [66]. This hypothesis was supported by the results of transplantation experiments in which isolated keratin 15-positive bulge stem cells were grafted to analyse cell fate plasticity, and it was demonstrated that they were capable of forming all cell linages, including hair follicles and sebaceous glands [19,66,68,72]. However, bulge stem cells are not the only cell source for sebaceous gland renewal. ...
... The mesenchymal portions of the hair follicle are comprised of the DP, an almond-shaped structure surrounded by the follicle bulb epithelium containing mesoderm-derived fibroblasts, and the DS, which is contiguous with the DP. Both DP and DS have been shown to play pivotal roles in hair follicle formation, growth, and support [100][101][102][103]. Multipotent stem cells within the dermis have been reported to originate in the DP and DS [104] that can differentiate into smooth muscle cells, neurons, glial cells, and adipocytes [105]. ...
... Additionally, DPCs serve an important role in the formation of HFs (7,8). Previous studies have demonstrated that the introduction of exogenous DPCs into the follicles induced the formation of new HFs in mice (9)(10)(11)(12). Furthermore, DPCs have been investigated as potential cell therapy for hair loss (13,14). ...
