Stephen Wisniewski's scientific contributions
Publications (2)
Acne vulgaris is the result of multifactorial disorders of the pilosebaceous duct. The initial lesion is believed to be hyper-keratinization of the infundibulum. The Rhino mouse has been used as an experimental acne model system for screening anti-keratinizing and comedolytic agents. Using this system we show that trypsin could induce desquamation...
Programmed cell death is a controlled process that leads to the elimination of single cells via apoptosis. Programmed cell death is fundamental to development, morphogenesis, and homeostasis. Proteases play a major role in the death process. We have previously shown that a serine protease, secreted by a keratinocyte cell line, can induce apoptosis...
Citations
... Trypsin is a serine peptidase that hydrolyses proteins and has physiological importance in food protein digestion in several invertebrate species (Muhlia-Almaz an et al. 2008). Other physiological functions have been described for trypsin, such as an important role in apoptosis in several organisms (Seiberg et al. 1997;Toubarro et al. 2009;Tian & Ouyang 2010). It has been suggested that the polymorphism of trypsin genes indicates diverse possibilities for regulating the expression of trypsin (Klein et al. 1998). ...
... As a consequence, there has been great interest in using the selective properties of enzymes and, thus, proteases have been examined for effectiveness in a number of such topical applications, including animal models of acne vulgaris, wound healing, epidermal ablation, and debridement of necrotic ulcers. Trypsin demonstrated antiaging properties and a comedolytic effect (i.e., opening up of clogged pores and lysis of comedones [hard plugs of keratin and sebum within hair follicles]) in a murine model of acne [11]. The principle physiological change that leads to acne vulgaris is the process of a sebaceous follicle transforming to a comedone via hypercornification and hyper-keratinization of the infundibulum (i.e., the funnel in which the hair follicle grows). ...
