H Gaissert's research while affiliated with German Cancer Research Center and other places

... Proteolytic enzymes applied by iontophoresis to skin of experimental animals have shown depilatory effects (Protopapa et al., 1999). As the depilatory action is long-lasting, the hypothesis had been put forward that hair follicle stem cells could be among the cells affected by the proteolytic enzyme. ...

... Thus, transgenic mice carrying only the E2 sequence of HPV11 were generated and the question was addressed whether expression of the viral protein will affect growth or differentiation of tissues, mainly skin, expressing the viral protein. This question is of high relevance in the light of experiments showing that the HPV11 promoter is able to drive specific expression of a reporter gene in the hair epithelium of transgenic mice ( Schenkel et al. 1999). In this report is demonstrated that the HPV11 E2 gene under the control of the human Ubiquitin C promoter is widely expressed in transgenic mice. ...

... The functions of the E6 and E7 oncogenes, which include their inactivation of the cellular tumor suppressors, p53 and pRB, respectively, have been well studied and their contribution to malignancy well-characterized. In contrast, the function of the E5 oncogene is less well understood; though multiple studies have implicated E5 in the viral life cycle ( Fehrmann et al., 2003;Genther et al., 2003) (Scott et al., 2019) as well as transformation/tumorigenesis (Bouvard et al., 1994;Crusius et al., 1998;Gu and Matlashewski, 1995;Leechanachai et al., 1992;Leptak et al., 1991;Pim et al., 1992;Straight et al., 1993;Tomakidi et al., 2000;Valle and Banks, 1995). HPV16 E5 is a hydrophobic, 83 amino acid membrane-associated protein with three transmembrane domains localized to multiple organelles in the cell, such as the Golgi apparatus, endoplasmic reticulum, and nuclear membrane ( Bubb et al., 1988;Conrad et al., 1993;Disbrow et al., 2003;Halbert and Galloway, 1988). ...

... The 5 0 segment contains the first E2bs, in addition to the transcription termination and polyadenylation sites for late transcripts. The central segment of the LCR is flanked by two E2bs and has been shown to function as an epithelial-specific transcriptional enhancer (22)(23)(24). This segment encloses several motifs that are important either to stimulate or to suppress viral transcriptional activity. ...