K Kobayashi's research while affiliated with Chiba University and other places
Publication (1)
The epidermis and its related appendages such as the hair follicle constitute the epithelial compartment of the skin. The exact location and distribution of the keratinocyte colony-forming cells within the epidermis or its appendages are unknown. We report that in the rat vibrissa, keratinocyte colony-forming cells are highly clustered in the bulge...
Citations
... When cultured on TCP in medium with or without serum, HDFs behaved individually and retained typical spindle-like morphologies, while HaCat cells tended to form colonies and their proliferation was dependent on both serum and cell seeding densities. As the keratinocyte colonies formed within epidermis, epidermal appendages, or recreated in 2D cell cultures, are subject to auto-regulation [28,29], the requirement of HaCat cells on high seeding density for colony formation and active proliferation can be explained as the genetically definable quantitative trait [30]. Within slow growing colonies, keratinocytes were observed to pack loosely, this could lead to the adjacent keratinocytes not forming the necessary coherence through the desmosomal junctions [31]; while the successful culture using high initial densities could be due to the short distances and extensive intercellular junctions between the cultivated keratinocytes [32]. ...
